scholarly journals Luminol Encapsulated Liposome as a Signal Generator for the Detection of Specific Antigen-antibody Reactions and Nucleotide Hybridization

2010 ◽  
Vol 26 (7) ◽  
pp. 767-772 ◽  
Author(s):  
Pakavadee RAKTHONG ◽  
Akarin INTARAMAT ◽  
Kavi RATANABANANGKOON
Keyword(s):  
Specific Antigen ◽  
Signal Generator ◽  
Antigen Antibody

Development of immunomagnetic droplet-based digital immuno-PCR for the quantification of prostate specific antigen

2018 ◽  
Vol 10 (29) ◽  
pp. 3690-3695 ◽  
Author(s):  
Wenhan Zhou
Keyword(s):  
Prostate Specific Antigen ◽  
Specific Antigen ◽  
Signal Generator ◽  
Antigen Antibody ◽  
Antibody Interactions

Immuno-PCR (IPCR) is an immunoassay that employs DNA as the signal generator and utilizes both the versatility of antigen–antibody interactions and the exponential amplification power of PCR.

Immunological Studies of Catechol Methyltransferase from the Yeast Candida tropicalis

1980 ◽  
Vol 35 (9-10) ◽  
pp. 712-716 ◽  
Author(s):  
Joseph Veser ◽  
Helmut Thomas
Keyword(s):  
Candida Tropicalis ◽  
Specific Antigen ◽  
Double Diffusion ◽  
Bovine Liver ◽  
Inhibitory Effect ◽  
Cross Reactivity ◽  
Diffusion Analysis ◽  
Potent Inhibitory Effect ◽  
Catechol Methyltransferase ◽  
Antigen Antibody

Abstract Immunization of rabbits with purified catechol methyltransferase from Candida tropicalis yielded a potent antiserum. Ouchterlony double diffusion analysis showed a single precipitin line. There was no cross reactivity with the catechol methyltransferase from rat and bovine liver. Specific antigen-antibody interaction was demonstrated by a potent inhibitory effect of the antibody on the yeast enzyme. Immunological titration and quantitative precipitin reaction of the enzyme showed that the maximum amount of precipitable complex occurred at the equivalence point where enzyme activity was completely inhibited.

Fast and Sensitive Measurement of Specific Antigen-Antibody Binding Reactions With Magnetic Nanoparticles and HTS SQUID

2009 ◽  
Vol 19 (3) ◽  
pp. 848-852 ◽  
Author(s):  
F. Oisjoen ◽  
J.F. Schneiderman ◽  
M. Zaborowska ◽  
K. Shunmugavel ◽  
P. Magnelind ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Specific Antigen ◽  
Antibody Binding ◽  
Antigen Antibody

scholarly journals SEROLOGICAL STUDIES IN RHEUMATIC FEVER

1949 ◽  
Vol 89 (6) ◽  
pp. 669-680 ◽  
Author(s):  
Edward E. Fischel ◽  
Ruth H. Pauli
Keyword(s):  
Rheumatic Fever ◽  
Complement Fixation ◽  
Specific Antigen ◽  
Phase Reaction ◽  
Tissue Extracts ◽  
Serological Studies ◽  
Specific Antigens ◽  
Wassermann Reaction ◽  
Antigen Antibody ◽  
And Control

1. An attempt was made to repeat and extend various tests which have been presumed to demonstrate specific antigens and antibodies in rheumatic fever. 2. The "phase reaction" appears to be an inconstant phenomenon probably related to a colloidal abnormality of the serum, rather than to a specific antigen-antibody system. 3. No specific autoantibodies to human tissue extracts were demonstrable by complement fixation or by the collodion particle technique. Variable results were noted with the same test sera on different occasions, and positive reactions with control tissues and control sera were observed. 4. The possibility should be considered that autoantibodies are not necessarily specific for rheumatic fever but may be manifestations of the occurrence of a type of reaction similar to a biologically false positive Wassermann reaction.

scholarly journals Enabling Indium Channels for Mass Cytometry by Using Reinforced Cyclambased Chelating Polylysine

2020 ◽  
Author(s):  
Laura Grenier ◽  
Maryline Beyler ◽  
Taunia L. L. Closson ◽  
Nick Zabinyakov ◽  
Alexandre Bouzekri ◽  
...  
Keyword(s):  
Cell Lines ◽  
Specific Antigen ◽  
The Other ◽  
Mass Cytometry ◽  
Stability Tests ◽  
Antibody Recognition ◽  
Cd20 Antibody ◽  
Anti Cd20 ◽  
Antigen Antibody

A metal containing polymer (MCP) based on a polylysine functionalized by In(III) chelates was synthesized. The chelator is based on a constrained dipicolinate cyclam that forms a highly inert In(III) complex. The MCP was conjugated to anti CD20 antibody using the very strong neutravidin/biotin interaction. Two cell lines, one expressing CD20 the other not, were stained with the modified antibody and analysed by mass cytometry using the In-115 channel. The results showed a specific antigen-antibody recognition and images by mass cytometry imaging could be obtained thanks to In-115 detection. Finally, overtime stability tests of the bioconjugate as well as multiplex experiments using the In-115 channel underline the high potentiel of this new In based MCP. <br><p></p>

scholarly journals Identification of Blood Group Antigens and Minor Cell Populations by the Fluorescent Antibody Method

Blood ◽  
1960 ◽  
Vol 15 (6) ◽  
pp. 884-900 ◽  
Author(s):  
FLOSSIE COHEN ◽  
WOLF W. ZUELZER ◽  
MARGARET M. EVANS
Keyword(s):  
Blood Group ◽  
Fluorescent Antibody ◽  
Specific Antigen ◽  
Cell Populations ◽  
Blood Group Antigens ◽  
Relative Paucity ◽  
The Difference ◽  
Antigen Antibody

Abstract It is possible to produce fluorescence of erythrocytes as the result of specific antigen-antibody reactions of various blood group antigens; thus far, the factors A and B, a variety of Rh antigens and Kidd, have been successfully demonstrated with this method. It is important to realize that in the presence of adequate negative controls, low intensity fluorescence like that obtained with Rh antigens is nevertheless specific in systems involving erythrocytes. The method discriminates between A1 and A2 cells. More antibody must be attached to the red cell for fluorescence than for agglutination. The relative paucity of antigenic sites of Rh substance as compared to A and B antigens is reflected by the difference in intensity of fluorescence. The fluorescent antibody technic has been used successfully for the demonstration, and, to some extent, quantitation of minor cell populations in in vitro mixtures and in vivo. Injected Rh-positive erythrocytes were demonstrated in the blood of an Rh-negative volunteer during a period of 20 days. Fetal Rh-positive erythrocytes were demonstrated in several Rh-negative women, both with and without antibody, in the last trimester of gestation.

scholarly journals Magnetic Carbon Nanotubes for Protein Separation

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Xiuhui Diao ◽  
Hongyu Chen ◽  
Guoliang Zhang ◽  
Fengbao Zhang ◽  
Xiaobin Fan
Keyword(s):  
Carbon Nanotubes ◽  
Protein Separation ◽  
Specific Antigen ◽  
Magnetic Systems ◽  
One Dimensional ◽  
Promising Strategy ◽  
Amidation Reaction ◽  
Aminophenylboronic Acid ◽  
Magnetic Carbon ◽  
Antigen Antibody

Magnetic separation is a promising strategy in protein separation. Owing to their unique one-dimensional structures and desired magnetic properties, stacked-cup carbon nanotubes (CSCNTs) with magnetic nanoparticles trapped in their tips can serve as train-like systems for protein separation. In this study, we functionalized the magnetic CSCNTs with high density of carboxyl groups by radical addition and then anchored 3-aminophenylboronic acid (APBA) through amidation reaction to achieve oriented conjunction of antibodies (IgG). It was also demonstrated that the obtained magnetic CSCNTs-APBA-IgG conjugates could readily react with target antigens through specific antigen-antibody reaction and be used as new magnetic systems for protein separation.

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