scholarly journals The Effect of Dipping Time in Natural Methyl Testosterone Hormone on Male Forming Succesiveness Process of Tilapia (Oreochromis niloticus) Larvae

2014 ◽  
Vol 10 (2) ◽  
Author(s):  
Rosmaidar Rosmaidar ◽  
Dwinna Aliza ◽  
Jesica Ramadhanita
Keyword(s):  
Statistical Analysis ◽  
Oreochromis Niloticus ◽  
Control Group ◽  
Forming Process ◽  
Average Percentage ◽  
Treatment Groups ◽  
Natural Hormone ◽  
Male Sex ◽  
Banda Aceh ◽  
Dipping Time

The purpose of this research was to know the influence of dipping time in natural methyl testosterone hormone (MT) on successful male forming process of tilapia (Oreochromis niloticus) larvae. This research used 360 7 days old tilapia larvae, purchased from cultivation of brackish water, Ujung Batee, Banda Aceh. The samples were divided into 4 treatment groups with three repetitions, they were P0 (control group without treatment), PII (Tilapia larvae were dipped in natural MT for 10 hours), PII (Tilapia larvae were dipped in natural MT for 20 hours), and PIII (Tilapia larvae were dipped in natural MT for 30 hours). All tilapia larvae were maintained until the age of 60 days, before the sex of the fish examined. The average percentage of male tilapia in group P0, PI, PII, and PIII were 50.28, 87.77, 90.06, and 95.05%, respectively. Statistical analysis showed that there were significant differences (P0.05) among P0, PI, PII, and PIII. Based on result of this research, it can be concluded that the longest dipping time the highest the percentage of male tilapia.Key words: larvae, tilapia, natural hormone methyl testosterone, the male sex percentage

scholarly journals 22. PENGARUH UMUR LARVA IKAN NILA (Oreochromis niloticus) TERHADAP PENINGKATAN PENJANTANAN MENGGUNAKAN HORMON METHYL TESTOSTERONE (MT) ALAMI Effect of Tilapia (Oreochromis niloticus) Age in Masculinization Process Using Methyl Testosterone (MT) Natural Hormones

2016 ◽  
Vol 8 (1) ◽  
Author(s):  
Rosmaidar R
Keyword(s):  
Key Words ◽  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Average Percentage ◽  
Natural Hormone ◽  
Nile Tilapia Oreochromis Niloticus ◽  
Male Sex ◽  
Natural Hormones

This research aims to know the influence of larvae of nile tilapia (Oreochromis niloticus) age on masculinization process by the administration of methyl testosterone (MT) natural hormones. This research used 270 nile tilapia larvae divided into 3 age of groups: 5 days (PI), 10 days (PII), and 20 days (PIII), with three repetitions. Larvae were dipped in MT natural hormone for 20 days then reared until age of 60 days, followed by observations of fish sex. The average percentage of male-sex of tilapia fish after dipping with methyl testosterone natural hormone on group PI, PII, and PIII were 68.07%, 66.47%, and 57.29%, respectively. It can be concluded that the age of larvae do not affect the percentage of masculinization process of nile tilapia fish. ____________________________________________________________________________________________________________________ Key words: larvae tilapia, masculinization, methyl testosterone natural hormone

scholarly journals The Effect of Various Dipping Times on Protein Level of Keumamah Fish

2014 ◽  
Vol 10 (2) ◽  
Author(s):  
Jumhuri Jumhuri ◽  
Ismail Ismail ◽  
Sulasmi Sulasmi
Keyword(s):  
Protein Level ◽  
Control Group ◽  
Fish Sample ◽  
Fish Protein ◽  
Treatment Groups ◽  
Banda Aceh ◽  
Dipping Time

The aim of this study was to find out protein level of Keumamah fish dipped in water for various times of dipping. This study used 1.5 kg of keumamah fish sample purchased from Peunayong Market Banda Aceh and divided into four treatment groups. P0 was control group without dipping, whereas P1, P2, and P3 were dipped in 100 C of water for 10, 20, and 30 minutes, respectively, with three repetitions each. The average of protein level on P0, P1, P2, and P3 was 13.66, 12.29, 11.8, and 10.56%, respectively. In conclusion, the dipping time affect to decrease keumamah fish protein level.Key words: keumamah, protein level, dipping time

scholarly journals Masculinization of Tilapia (Oreochromis niloticus) Using Methyl Testosterone Natural Hormone

2014 ◽  
Vol 10 (2) ◽  
Author(s):  
Rosmaidar R ◽  
Winaruddin W ◽  
Mimi Herlina
Keyword(s):  
Oreochromis Niloticus ◽  
Fish Larvae ◽  
Hormone Treatment ◽  
Control Fish ◽  
Control Group ◽  
Group Iii ◽  
Randomized Design ◽  
Natural Hormone ◽  
Male Sex ◽  
Completely Randomized Design

This study aimed to determine the effect of methyl testosterone natural hormoneon the rate of masculinization of tilapia (Oreochromis niloticus). The study used 270 tilapia larvae with the age of 7 days as experimental animals. Tilapia larvae were divided into 3 treatments; eachtreatment consisted of 30 larvae fish. This study was an experimental study using completely randomized design with 3 treatments and 3replications. Treatment I as control group was fed with pellets ad libitum, treatment II was given feed containing methyl testosterone naturalhormone, and treatment group III was given hormone treatment by soaking the fish larvae with methyl testosterone natural hormone for 20hours. Larvae were raised in accordance with applicable procedures. Observation was conducted when the larvae fish were at 60 days old andthe sex of fish can be observed. Parameter examined was the percentage of the male sex. The average (± SD) percentage of male tilapia in groupPI, PII, and PIII were 36.39±3.97, 66.72±6.13, and 82.37±6.87%, respectively. The results showed that the percentage of male sex of tilapia fedwith natural hormone of methyl testosterone through the diet and dipping was significantly different (P0.05) from control fish. In conclusion,the administration of natural hormone of methyl testosterone increased the percentage of the male sex of tilapia).____________________________________________________________________________________________________________________Key words: tilapia larvae, natural hormone methyl testosterone, the male sex percentage

scholarly journals POTENTIAL APPLICATIONS OF DIETARY Moringa oleifera LEAVES AS GROWTH MODULATOR AND IMMUNOSTIMULANT AGAINST Aeromonas hydrophila FOR FARMED Oreochromis niloticus

2021 ◽  
Vol 16 (2) ◽  
pp. 109
Author(s):  
Ratchaneegorn Mapanao ◽  
Wirat Jiwyam ◽  
Wilailuk Khrueanet ◽  
Nudtha Nithikulworawong
Keyword(s):  
Disease Resistance ◽  
Growth Performance ◽  
Aeromonas Hydrophila ◽  
Oreochromis Niloticus ◽  
Blood Cells ◽  
Nile Tilapia ◽  
Moringa Oleifera ◽  
Control Group ◽  
Blood Cell Count ◽  
Treatment Groups

Herbal medicine, including moringa (Moringa oleifera), is widely used as dietary supplementation to enhance growth performance and increase disease resistance in aquaculture. This study aimed to investigate the effects of supplementing moringa leaves on growth performance, hematology and disease resistance of nile tilapia (Oreochromis niloticus) against Aeromonas hydrophila. Fish (38.05 ± 0.83 g) were fed with a commercial feed supplemented by moringa leaves at 0%, 15%, 20%, and 25%, ad libitum, twice a day for eight weeks. Results revealed that 15% moringa leaves supplemented diet gave the highest weight gain, while the specific growth rate was statistically significantly different (p<0.05) among the treatments. Fish fed with 25% supplementation had the highest total red blood cell count and total white blood cell count. All experimental groups had higher values of red blood cells and white blood cells than the control group. The challenge test with A. hydrophila showed that the fish fed with the 25% moringa leaves supplementation diet had the highest relative percentage survival rate. There were no significant differences among the treatment groups. However, the treatment groups had a statistically significant difference with the control group (p<0.05). Results of the present study indicated that supplementation of 15% moringa leaves in diet gave optimal growth performance, while supplementation of 25% moringa leaves in the diet showed the best results in terms of the health of nile tilapia. Moringa leaves supplemented in nile tilapia diet enhanced growth performance and increased disease resistance against A. hydrophila.

PENGARUH LINAMARIN TERHADAP PENAMPILAN REPRODUKSI INDUK MENCIT (Mus musculus L.)

2017 ◽  
Vol 11 (4) ◽  
pp. 289
Author(s):  
Fitra Wahyuni ◽  
Sony Heru Sumarsono
Keyword(s):  
Body Weight ◽  
Statistical Analysis ◽  
Reproductive Performance ◽  
Fetal Death ◽  
Control Group ◽  
Average Weight ◽  
Treatment Groups ◽  
Pregnant Mice ◽  
And Control ◽  
Average Weight Gain

<p align="center"><strong><em>ABSTRACT</em></strong></p><p><em>This study was performed to observe the effect of linamarin on reproductive performance, that consist of the number of live fetuses, reabsorption embryo or fetal, fetal death and fetal body weight together with length. Pregnant mice were divided into one control group with administration of distilled water (0 mg/kgb.w/days) and six treatment groups linamarin dose is 0.36; 0.72; 1.26 mg; 2; 4; and 8 mg/kgb.w./days. Linamarin dosing and control begins at 5<sup>th</sup> to 16<sup>th</sup> gestation days, at 17-days of gestation caesarean section were performed to pregnant mice to remove foetuses. Statistical analysis used was a variation analysis by ANOVA followed by comparisons between treatments HSD test (Tukey). Giving linamarin observed in the parent mice led to a decrease in average weight gain of the mother during pregnancy compared with controls. In linamarin dose 8 mg/kgb.w/day an increase in embryo reabsorption and fetal death, as well as the observed decrease in fetal body weight and length, but after statistical analysis of the decrease was not significantly different. Linamarin with the doses given in this study is not expected to interfere with pregnancy based on reproductive performance were observed.</em></p><p><em> </em></p><p><strong><em>Keywords:</em></strong><em> Linamarin, reproductive performance, fetal weight, fetal body length</em></p><p><em> </em></p><p align="center"><strong><em>ABSTRAK</em></strong></p><p><em>Penelitian ini dilaksanakan bertujuan untuk mengamati pengaruh linamarin pada penampilan reproduksi yang meliputi jumlah fetus hidup, embrio resorpsi, fetus mati dan berat beserta panjang badan fetus. Induk mencit yang bunting dikelompokkan ke dalam satu kelompok kontrol dengan pemberian akuades (0 mg/kgb.b./hari) dan enam kelompok perlakuan dosis linamarin yaitu 0,36; 0,72; 1,26 mg; 2; 4; dan 8 mg/kgb.b./hari. Pemberian dosis linamarin dan kontrol dimulai pada umur kebuntingan 5 sampai 16 hari, setelah itu pada umur kebuntingan 17 hari mencit dikorbankan nyawanya untuk pengambilan fetus. Analisis statistik yang digunakan adalah analisis variasi dengan ANOVA dan dilanjutkan dengan uji perbandingan antar perlakuan HSD (Tukey). Pemberian linamarin pada induk mencit diamati menyebabkan penurunan rata-rata pertambahan berat badan induk selama kebuntingan dibandingkan dengan kontrol. Pada dosis linamarin 8 mg/kgb.b./hari terjadi peningkatan embrio resorpsi dan fetus yang mati, serta diamati penurunan berat dan panjang badan fetus, namun setelah dilakukan analisis statistik penurunan tersebut tidak berbeda nyata. Linamarin dengan dosis yang diberikan pada penelitian ini diduga tidak mengganggu kebuntingan berdasarkan penampilan reproduksi yang diamati.</em></p><p><em> </em></p><p><strong><em>Kata kunci:</em></strong><em> Linamarin, penampilan reproduksi, berat badan fetus, panjang badan fetus</em></p>

scholarly journals Effect of Temperature Increase on Hemoglobin Concentration and Hematocrit Value of Tilapia Fish (Oreochromis niloticus)

2008 ◽  
Vol 8 (1) ◽  
Author(s):  
Zuhrawati N. A.
Keyword(s):  
Oreochromis Niloticus ◽  
Temperature Increase ◽  
Hemoglobin Concentration ◽  
Effect Of Temperature ◽  
Control Group ◽  
Blood Samples ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Fish Hemoglobin ◽  
Banda Aceh

This research aims to determine the effect of temperature increase on hemoglobin consentration and hematocrit value of tilapia fish. This research was conducted at Faculty of Veterinary Medicine Syiah Kuala University Banda Aceh. Samples used were blood from tilapia fish thatderived from 12 tilapia fish with weight range of 40-50 g. The research design used was a completely randomized design (CRD) unidirectionalpattern with 3 treatments and 4 replications. P1 as a control group was the treatment with aquarium temperature of 29±1C, P2 was thetreatment with aquarium temperature of 32±1o C, and P3 was the treatment with aquarium temperature of 35±1o C. All aquarium size in thisstudy was 80 cm x 60 cm x 40 cm with the height of water 30 cm. The treatment was carried out for 15 days and on day 16 blood samples werecollected. Blood was taken through the caudalis vein. Data were analyzed by analysis of variance (ANOVA) one way, followed by Tukey test.Mean (± SD) of tilpia fish hemoglobin levels (g/dl) on P1, P2, and P3 were 4.50±0.49, 5.08±0.79, and 6.08±0.94, respectively. Mean (± SD) oftilapia fish hematocrit value (%) in P1, P2, and P3 consecutively were 26.50±8.10, 33.25±11.78, and 40.25±9.04. Statistical analysis showed thatan increase in temperature showed no effect (P 0.05) to the hemoglobin levels of tilapia fish and highly significant to the hematocrit value (P0.01). It can be concluded that the increase of water temperature on 32±1° C and 35±1° C in can increase the hemoglobin levels andhematocrit value of tilapia fish.____________________________________________________________________________________________________________________Key words: tilaphia fish, hemoglobin levels, hematocri value, temperature o

scholarly journals THE EFFECT OF TOMAN (Chana Micropetes) EXTRACT ON THE IMPROVEMENT OF ALBUMIN LEVELS IN MENCIT/MICE (Mus musculus)

2020 ◽  
Vol 3 (2) ◽  
pp. 66
Author(s):  
Pipit Festi W
Keyword(s):  
Body Weight ◽  
Mus Musculus ◽  
Control Group ◽  
Control Groups ◽  
Control Group Design ◽  
Treatment Groups ◽  
Group Design ◽  
Average Value ◽  
Male Sex ◽  
Alternative Diet

Hypoalbuminemia is a condition in which albumin levels are less than 3.8 g / dl. Management of hypoalbumin can be done on a  high high in protein such as toman fish extract (Chana Micropetes). Toman fish extract contains globular protein which can be used as an alternative diet in increasing albumin levels. The purpose of this study was to determine the effect of toman fish extract on increasing albumin levels in mencit (Mus musculus).               This research is an experimental research design with Pretest-Posttest Control Group Design. Samples using Mus musculus with the criteria of male sex, body weight 25-40 grm and 2 months old. The number of samples consisted of 32 animals consisting of 2 groups, 16 control groups and 16 treatment groups. Intervention by giving toman fish extract for 14 days. Research instruments, albumin levels and observation sheets. Data were tested statistically with Independent Sample t Test.               The results, this study showed the average value of albumin levels was 5.60 gr / dl in the treatment group so that an increase in albumin levels. There was a significant effect (p = 0,000 <α = 0.05) between the administration of toman fish extract to the increase in albumin levels in mencit (Mus Muculus).               Conclusion, giving toman fish extract can increase albumin levels in mencit. So that toman fish extract can be used as a high-protein alternative diet for hypoalbumin sufferers.Keywords: hypoalbumin, toman fish, (Chana Micropetes).

scholarly journals PENGARUH UMUR TERHADAP PENJANTANAN LARVA IKAN LELE DUMBO (Clarias gariepinus) YANG DIRENDAM MENGGUNAKAN HORMON METIL TESTOTERON ALAMI (The Influence of Age on Masculinization of Clarias gariepinus Larvae Immersed by Methyl Testosterone Natural Hormone)

2016 ◽  
Vol 10 (1) ◽  
pp. 41
Author(s):  
Hattanul Mulia ◽  
Rosmaidar Rosmaidar ◽  
Dasrul Dasrul ◽  
Dwinna Aliza ◽  
Dian Masyitha ◽  
...  
Keyword(s):  
Analysis Of Variance ◽  
Clarias Gariepinus ◽  
Age Group ◽  
Random Design ◽  
Average Percentage ◽  
Group I ◽  
Natural Hormone ◽  
Male Sex

This research aims to examines the influence of age on masculinization of Clarias gariepinus larvae immersed by methyl testosterone (MT) natural hormone. This research used 270 catfish larvae divided into 3 groups of age those are 5, 10, and 20 days. The research implemented complete random design (CRD). Immersion was performed using methyl testosterone hormone at the dose of 0.25 g/L for 20 hours. Observation of fish was done after 60 day-old fish. Data obtained was analyzed using one-way analysis of variance The average percentages of male-sex catfish after immersing in natural MT on group I, II, and III were 97.67, 91.07, and 86.67%, respectively. The results showed that the age of the larvae did not affect significantly (P>0.05) on the percentage of male sex of Clarias gariepinus. The average percentage of male sex in 5 days age group was higher than 10 and 20 days age group. In conclusion, the masculinization of Clarias gariepinus larva immersed by MT natural hormone is not influenced by age of fish before dipping.

Changes of IL-4 and IFN-g expression in monocytes and T-helper lymphocytes while modeling of systemic juvenile idiopathic arthritis in Wistar rats

2018 ◽  
pp. 61-69
Author(s):  
В.Н. Сахаров ◽  
П.Ф. Литвицкий ◽  
Е.И. Алексеева ◽  
Н.А. Маянский ◽  
Р.Ш. Закиров
Keyword(s):  
Juvenile Idiopathic Arthritis ◽  
Peripheral Blood Mononuclear Cells ◽  
Wistar Rats ◽  
Mononuclear Cells ◽  
Systemic Juvenile Idiopathic Arthritis ◽  
Control Group ◽  
Peripheral Blood Mononuclear ◽  
Treatment Groups ◽  
Blood Mononuclear Cells ◽  
T Helpers

Цель исследования - изучение перепрограммирования мононуклеарных лейкоцитов на модели системного ювенильного идиопатического артрита (сЮИА), воспроизводимой у крыс Wistar с использованием полного адъюванта Фрейнда и липополисахарида. Методика. сЮИА воспроизведен у 6-месячных крыс-самцов Wistar. На 40-е сут. эксперимента животные были разделены на 3 группы: 1-я группа - контроль; 2-я - группа доксициклина; 3-я - группа дексаметазона. Взятие проб крови у животных проводили на нулевые, 41-е и 55-е сут. Мононуклеарные клетки периферической крови выделяли гравиметрически, после чего окрашивали их на маркеры и внутриклеточные цитокины. Дифференцировали моноциты (CD3-CD4+) и Т-хелперы (CD3+CD4+). Анализировали динамику внутриклеточной экспрессии интерлейкина IL-4 (рассматривали как маркер про-М2 фенотипа, так как в случае выделения из клетки ИЛ-4 служит стимулятором М2 поляризации макрофагов) и IFN-g (как маркер про-М1 фенотипа) по данным проточной цитофлуориметрии. Применяли непараметрический статистический тест Mann-Whitney-Wilcoxon в программе R для статистической обработки данных. Результаты и заключение. При моделировании сЮИА выявлено закономерное изменение фенотипа моноцитов. Применение же доксициклина и дексаметазона приводило к более ранней поляризации их по про-М2-пути в отношении моноцитов (на 41-е сут.) в сравнении с контролем. Про-М1 эффект (на 55-е сут., в сравнении с контролем) выявлен также в группах доксициклина и дексаметазона. У животных разных групп обнаружены характерные динамические изменения внутриклеточной экспрессии цитокинов. Важно, что различная направленность поляризации фенотипа при сЮИА и применении препаратов наблюдается не только у моноцитов, но и у Т-хелперов. The study objective was to evaluate targeted reprogramming of peripheral blood mononuclear cells in systemic juvenile idiopathic arthritis (sJIA) modeled in 6-month-old male Wistar rats by co-administration of complete Freund’s adjuvant and lipopolysaccharide. Methods. On day 40 of the experiment, rats were divided into three groups: control, doxycycline, and dexamethasone groups. Blood samples were collected on days 0, 41, and 55. Peripheral blood mononuclear cells were isolated gravimetrically and stained for markers and cytokines. Monocytes (CD3-CD4+) and T-helpers (CD3+CD4+) were differentiated as target cells. IL-4 was considered a marker for the pro-M2 phenotype since IL-4 can activate M2 macrophage polarization; IFN-g was considered a marker for the pro-M1 phenotype. Time-related changes in the intracellular expression of IL-4 and IFN-g were studied using flow cytometry. Data were analyzed using nonparametric statistical tests (Mann-Whitney-Wilcoxon) in the R environment for statistical computing. Results and conclusions. Monocytes (like macrophages) underwent reprogramming during the development of modeled sJIA disease. In monocytes of doxycycline and dexamethasone treatment groups, pro-M2 effects were observed earlier (day 41) than in the control group. Pro-M1 effects were observed in monocytes of doxycycline and dexamethasone groups on day 55, as compared with the control group. Characteristic time-related changes of intracellular cytokine expression were described for different groups. Importantly, the differently directed phenotype polarization was observed in sJIA and treatment groups for both monocytes and T-helpers.

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