scholarly journals Poly(3-hydroxybutyrate-co-4-hydroxybutyrate) Produced by Delftia acidovorans in Different Cell-Concentration and Fed-Batch Cultures

2004 ◽  
Vol 60 (3) ◽  
pp. 95-98 ◽  
Author(s):  
Wen-chuan Hsieh ◽  
Yuki Wada ◽  
Hiroshi Mitomo
Keyword(s):  
Cell Concentration ◽  
Fed Batch ◽  
Batch Cultures ◽  
Delftia Acidovorans

scholarly journals Development of Processes for Recombinant L-Asparaginase II Production by Escherichia coli Bl21 (De3): From Shaker to Bioreactors

Pharmaceutics ◽  
2020 ◽  
Vol 13 (1) ◽  
pp. 14
Author(s):  
Thaís Barros ◽  
Larissa Brumano ◽  
Marcela Freitas ◽  
Adalberto Pessoa ◽  
Nádia Parachin ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Cell Concentration ◽  
Culture Media ◽  
Defined Medium ◽  
Batch Cultivation ◽  
Lymphocytic Leukemia ◽  
Fed Batch ◽  
Batch Cultures ◽  
Lactose Induction ◽  
Asparaginase Activity

Since 1961, L-asparaginase has been used to treat patients with acute lymphocytic leukemia. It rapidly depletes the plasma asparagine and deprives the blood cells of this circulating amino acid, essential for the metabolic cycles of cells. In the search for viable alternatives to produce L-asparaginase, this work aimed to produce this enzyme from Escherichia coli in a shaker and in a 3 L bioreactor. Three culture media were tested: defined, semi-defined and complex medium. L-asparaginase activity was quantified using the β-hydroxamate aspartic acid method. The defined medium provided the highest L-asparaginase activity. In induction studies, two inducers, lactose and its analog IPTG, were compared. Lactose was chosen as an inducer for the experiments conducted in the bioreactor due to its natural source, lower cost and lower toxicity. Batch and fed-batch cultures were carried out to reach high cell density and then start the induction. Batch cultivation provided a final cell concentration of 11 g L−1 and fed-batch cultivation produced 69.90 g L−1 of cells, which produced a volumetric activity of 43,954.79 U L−1 after lactose induction. L-asparaginase was produced in a shaker and scaled up to a bioreactor, increasing 23-fold the cell concentration and thus, the enzyme productivity.

Using Alternating Tangential Flow with Fed-Batch Cultures

2019 ◽  
Vol 39 (10) ◽  
pp. 54-56
Author(s):  
Shashi Kudugunti ◽  
Daniel Diggins ◽  
Jyoti Amatya ◽  
Jamie Peyser
Keyword(s):  
Fed Batch ◽  
Batch Cultures ◽  
Tangential Flow

scholarly journals A novel process-based model of microbial growth: self-inhibition in Saccharomyces cerevisiae aerobic fed-batch cultures

2015 ◽  
Vol 14 (1) ◽  
Author(s):  
Stefano Mazzoleni ◽  
Carmine Landi ◽  
Fabrizio Cartenì ◽  
Elisabetta de Alteriis ◽  
Francesco Giannino ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Microbial Growth ◽  
Fed Batch ◽  
Batch Cultures

Ethanol production by anaerobic thermophilic bacteria: Kinetics in fed-batch cultures ofClostridium thermohydrosulfuricum

1989 ◽  
Vol 11 (6) ◽  
pp. 443-448 ◽  
Author(s):  
Fatiou Toukourou ◽  
Luiz Donaduzzi ◽  
Andr� Miclo ◽  
Pierre Germain
Keyword(s):  
Ethanol Production ◽  
Thermophilic Bacteria ◽  
Fed Batch ◽  
Batch Cultures
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