Comparison of Different Dna Preservation Methods On Quinqueloculina Spp. (foraminifera, Miliolida)

2018 ◽  
Vol 48 (3) ◽  
pp. 186-192
Author(s):  
Man Lyu ◽  
Yanli Lei ◽  
Tiegang Li
Keyword(s):  
Rose Bengal ◽  
Ethylenediaminetetraacetic Acid ◽  
Experimental Setup ◽  
Air Drying ◽  
Dna Preservation ◽  
Preservation Methods ◽  
Series Of Experiments ◽  
Lysis Buffer ◽  
Edta Ethylenediaminetetraacetic Acid

Abstract We carried out a series of experiments to evaluate the efficiency of preserving DNA from porcelaneous foraminifera (Quinqueloculina spp.) and a second set to assess the effect of Rose Bengal staining on molecular processing. The first experimental setup assessed three methods of DNA preservation (air-drying, freezing with or without seawater, and Guanidine lysis buffer treatment with or without EDTA (Ethylenediaminetetraacetic acid)). Our study produced the following results: 1) there were no significant differences in DNA preservation when samples were air dried across a range of temperatures (20–120°C); samples frozen at −20°C appeared better preserved than at those frozen at –80°C, and freezing without seawater appeared to produce better preservation than with seawater, though differences in freezing treatments were not significant (p > 0.05); samples in Guanidine lysis buffer with EDTA and stored at –20°C were well preserved (p < 0.05); 2) sometimes, DNA was successfully extracted from samples stained with Rose Bengal. We recommend Guanidine lysis buffer with EDTA, stored at –20°C for up to six weeks, as the best protocol for preservation of DNA from porcelaneous foraminifera.

scholarly journals Compact Laser Collimation System for Simultaneous Measurement of Five-Degree-of-Freedom Motion Errors

2020 ◽  
Vol 10 (15) ◽  
pp. 5057
Author(s):  
Chuang Sun ◽  
Sheng Cai ◽  
Yusheng Liu ◽  
Yanfeng Qiao
Keyword(s):  
Simultaneous Measurement ◽  
Measurement Accuracy ◽  
Measurement Data ◽  
Optical Path ◽  
Degree Of Freedom ◽  
Experimental Setup ◽  
Collimation System ◽  
Optical Configuration ◽  
Series Of Experiments ◽  
The Common

A compact laser collimation system is presented for the simultaneous measurement of five-degree-of-freedom motion errors. The optical configuration of the proposed system is designed, and the principle of the measurement of five-degree-of-freedom errors is described in detail. The resolution of the roll and the horizontal straightness is doubled compared with other laser collimation methods. A common optical path compensation method is provided to detect light drift in real time and compensate for straightness and angle errors. An experimental setup is constructed, and a series of experiments are performed to verify the feasibility and stability of the system. Compared with commercial instruments, the pitch and yaw residuals are ± 2.5 ″ and ± 3.5 ″ without correction, and the residuals are ± 1.9 ″ and ± 2.8 ″ after correction, respectively. The comparison deviations of the horizontal straightness and vertical straightness changed from ± 4.8   μ m to ± 2.8 μm and ± 5.9 μm to ± 3.6 μm, respectively. The comparison deviation of the roll is ± 4.3 ″ . The experimental results show that the data of the five-degree-of-freedom measurement system obtained are largely the same as the measurement data of commercial instruments. The common optical path compensation can effectively improve the measurement accuracy of the system.

scholarly journals Neuronal Hyperexcitability and Reduction of GABAA-Receptor Expression in the Surround of Cerebral Photothrombosis

1996 ◽  
Vol 16 (5) ◽  
pp. 906-914 ◽  
Author(s):  
Klaus Schiene ◽  
Claus Bruehl ◽  
Karl Zilles ◽  
Meishu Qu ◽  
Georg Hagemann ◽  
...  
Keyword(s):  
Gabaa Receptor ◽  
Rose Bengal ◽  
Sensorimotor Cortex ◽  
Neuronal Excitability ◽  
Epileptic Seizures ◽  
Receptor Expression ◽  
Aminobutyric Acid ◽  
Brain Areas ◽  
Series Of Experiments ◽  
The Rose

Changes of neuronal excitability and γ-aminobutyric acid (GABAA)-receptor expression were studied in the surround of photothrombotic infarcts, which were produced in the sensorimotor cortex of the rat by using the rose bengal technique. In a first series of experiments, multiunit recordings were performed on anesthetized animals 2–3 mm lateral from the lesion. Mean discharge frequency was considerably higher in recordings from lesioned animals (>100 Hz in the first postlesional week) compared with control animals (mean, 15 Hz). These alterations were already present after 1 day but were most pronounced 3 to 7 days after lesion induction. Thereafter the hyperexcitability declined again, although it remained visible up to 4 months. In a second series of experiments, the GABAA-receptor expression was studied autoradiographically. This revealed a reduction of GABAA receptors in widespread brain areas ipsilateral to the lesion. The reduction was most pronounced in the first days after lesion induction and declined with longer intervals. It is concluded that cortical infarction due to photothrombosis leads to a long-lasting and widespread reduction of GABAA-receptor expression in the surround of the lesion, which is associated with an increased neuronal excitability. Such alterations may be responsible for epileptic seizures that can be observed in some patients after stroke and may contribute to neurologic deficits after stroke.

Development of an Experimental Setup for the Investigation of Grinding of Wood and a Proposal for a Pulsed Loading Technique

1978 ◽  
Vol 100 (2) ◽  
pp. 147-152
Author(s):  
D. Dornfeld ◽  
S. M. Wu
Keyword(s):  
Wood Properties ◽  
Control Device ◽  
Variable Frequency ◽  
Experimental Setup ◽  
Stone Surface ◽  
Laboratory Equipment ◽  
Surface Conditions ◽  
Pulsed Loading ◽  
Series Of Experiments ◽  
Grinding Operations

The study of the fiberizing mechanism in the grinding of wood requires specialized laboratory equipment to allow process alteration and data collection. The design and development of a unique grinding setup and the control device and instrumentation needed for wood grinding research are outlined. A pulsed loading technique for increasing the effectiveness of the fiberizing mechanism is proposed and this technique is tested in a series of experiments conducted with the grinding operations. Significant energy saving and pulp quality improvement for wood ground at 72 F (22.2 C) are achieved using a variable frequency and amplitude load pulsation superimposed over the constant grinding load. The pulsed loading technique can be used as a control device to minimize the effect of grinder input variation such as wood properties or stone surface conditions by altering the process during grinding to optimize the effect of the fiberizing mechanism.

Control of Zn, Cu and Mn activities in alkaline and calcareous soils equilibrated with EDTA

1982 ◽  
Vol 98 (1) ◽  
pp. 203-207 ◽  
Author(s):  
S. P. Singh
Keyword(s):  
Metal Ion ◽  
Ethylenediaminetetraacetic Acid ◽  
Ph Dependence ◽  
Calcareous Soils ◽  
Transition Metal Ions ◽  
Ion Activities ◽  
Solid Phases ◽  
Equilibrium Mole Fraction ◽  
Edta Ethylenediaminetetraacetic Acid

SUMMARYThe modified EDTA (ethylenediaminetetraacetic acid) method of determining the ionic activities in soils developed by Norvell was used to measure Zn2+, Cu2+ and Mn2+ activities including the competition of Ca and Mg for the chelating ligand EDTA. In the Zn-EDTA system, the equilibrium mole fraction of CaL2− + MgL2− increased with an increase in pH of the soil solution, thereby leading to a decrease in the concentration of transition metal ions. In view of the pH dependence of the metal ion activities, the values of the expression pM + 2 pOH (where M may be Zn, Cu or Mn) were used to discuss the possible role of various oxides, hydroxides, carbonates, sulphides and unknown solid phases in regulating the activities of Zn2+, Cu2+ and Mn2+ ions in alkaline and calcareous soils.

scholarly journals An Experimental and Numerical Analysis of Puff Hydrodynamics

2004 ◽  
Vol 21 (3) ◽  
pp. 157-166 ◽  
Author(s):  
MS Saidi ◽  
MR Hajaligol ◽  
F Rasouli
Keyword(s):  
Fluid Dynamics ◽  
Numerical Simulation ◽  
Computational Fluid Dynamics ◽  
Air Flow ◽  
Experimental Setup ◽  
Gas Viscosity ◽  
Flow Through ◽  
Series Of Experiments ◽  
Burn Rate ◽  
Centerline Temperature

AbstractThe permeability of a tobacco rod in a cigarette increases as it converts into char and ash in the coal. The hot coal introduces a significant resistance to the air flow when air passes through. Through a series of experiments, the cigarette burn line and burn rate, the centerline temperature, and the pressure drop were measured for continuous puffing conditions. The gas viscosity was calculated from the temperature distribution inside the cigarette and applying Sutherland's law. Then, the experimental setup was mathematically modeled from a commercially available CFD (Computational Fluid Dynamics) code and, by matching the numerical and experimental results, the changes in coal and filter permeability during puffing were estimated. The numerical simulation successfully reproduced the results of experiments on the air flow through the coal, ventilation holes and paper wrapper.

scholarly journals PENGARUH WAKTU DAN PELARUT EDTA (Ethylenediaminetetraacetic Acid) PADA EKSTRAKSI FUKOIDAN DARI RUMPUT LAUT COKELAT Sargassum binderi Sonder

2020 ◽  
Vol 8 (03) ◽  
pp. 265
Author(s):  
Cindy Rizka Aulia
Keyword(s):  
Ethylenediaminetetraacetic Acid ◽  
Sargassum Binderi ◽  
Crude Fucoidan ◽  
Edta Ethylenediaminetetraacetic Acid

Rumput laut cokelat memiliki beragam polisakarida yang terkandung di dalamnya. Salah satu polisakarida dari rumput laut cokelat yang diketahui memiliki bioaktivitas yang tinggi adalah fukoidan. Banyak penelitian telah menunjukkan bahwa bioaktivitas dari fukoidan antara lain mampu menghambat pertumbuhan sel kanker, anti koagulan, anti virial, dan sebagai immunostimulan. Mengisolasi fukoidan dari rumput laut cokelat telah banyak dilakukan dengan beragam cara. Penelitian ini bertujuan untuk mengetahui bagaimana pengaruh waktu dan konsentrasi pelarut EDTA (ethylenediaminetetraacetic acid) pada proses ekstraksi dan karakteristik fukoidan dari rumput laut cokelat jenis Sargassum binderi Sonder. Konsentrasi pelarut yang digunakan pada penelitian ini sebesar 0,15% ; 0,3%: 0,5%; 1%; dan 1,3% serta waktu ekstraksi selama 1 jam, 2 jam, 3 jam, 4 jam, dan 5 jam. Perbandingan rumput laut dan air adalah 1:30 dengan suhu ekstraksi dilakukan pada 70˚C. Dalam penelitian ini didapatkan yield crude fucoidan terbesar pada waktu ekstraksi 4 jam dan konsentrasi pelarut 1,3% yaitu sebesar 3,67%. Hasil analisis menunjukkan kandungan total gula dari ekstrak fukoidan dengan yield terbesar didapatkan sebesar 36,48% dan kandungan sulfat sebesar 17,16%.

scholarly journals Molecular Evidence for Flea-Borne Rickettsiosis in Febrile Patients from Madagascar

Pathogens ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 1482
Author(s):  
Christian Keller ◽  
Raphael Rakotozandrindrainy ◽  
Vera von Kalckreuth ◽  
Jean Noël Heriniaina ◽  
Norbert Georg Schwarz ◽  
...  
Keyword(s):  
Ethylenediaminetetraacetic Acid ◽  
Molecular Data ◽  
Antimicrobial Treatment ◽  
Accurate Diagnosis ◽  
The Other ◽  
Molecular Evidence ◽  
Rickettsial Infection ◽  
Blood Samples ◽  
Edta Ethylenediaminetetraacetic Acid

Rickettsiae may cause febrile infections in humans in tropical and subtropical regions. From Madagascar, no molecular data on the role of rickettsioses in febrile patients are available. Blood samples from patients presenting with fever in the area of the capital Antananarivo were screened for the presence of rickettsial DNA. EDTA (ethylenediaminetetraacetic acid) blood from 1020 patients presenting with pyrexia > 38.5 °C was analyzed by gltA-specific qPCR. Positive samples were confirmed by ompB-specific qPCR. From confirmed samples, the gltA amplicons were sequenced and subjected to phylogenetic analysis. From five gltA-reactive samples, two were confirmed by ompB-specific qPCR. The gltA sequence in the sample taken from a 38-year-old female showed 100% homology with R. typhi. The other sample taken from a 1.5-year-old infant was 100% homologous to R. felis. Tick-borne rickettsiae were not identified. The overall rate of febrile patients with molecular evidence for a rickettsial infection from the Madagascan study site was 0.2% (2/1020 patients). Flea-borne rickettsiosis is a rare but neglected cause of infection in Madagascar. Accurate diagnosis may prompt adequate antimicrobial treatment.

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