scholarly journals Molecular Diagnosis of Human Metapneumovirus in Hospitalized Children with Acute Respiratory Tract Infections using RT-LAMP: A Population-Based Prospective Study

2021 ◽  
Vol 11 (2) ◽  
pp. 146-150
Author(s):  
Ahmed Osman Gasim Attar ◽  
Khalid Enan ◽  
Sara Abdelghani ◽  
Lienda Bashier Eltayeb
Keyword(s):  
Respiratory Tract ◽  
Respiratory Tract Infections ◽  
Clinical Symptoms ◽  
Diagnostic Technique ◽  
Lamp Assay ◽  
Human Metapneumovirus ◽  
Hospitalized Children ◽  
Age Group ◽  
Acute Respiratory Tract Infections ◽  
Tract Infections

Background: Human metapneumovirus (hMPV) is a major novel cause of acute respiratory infections ranging from wheezing to bronchiolitis and pneumonia in children worldwide. The aim of this study was to detect hMPV in hospitalized children with acute respiratory tract infections (ARTIs) by using reverse transcription-loop mediated isothermal amplification (RT-LAMP) assay. Methods and Results: A total of 68 children with ARTIs who were clinically suspected of acquiring hMPV were included in the study in the period between January 2019 and February 2020. Posterior-pharyngeal (throat) swabs were obtained from each patient. hMPV RNA was revealed in 18(26.5%) cases. The age range was from <1 year to 10 years (mean age of 5.25±2.62). Sixteen (23.5%) of the participants were in the age group of <1 year, where the majority of hMPV-positive subjects (n=11) were found (16.2% of the total number of infected children) (P=0.0025). The majority of hMPV-negative subjects (n=15) were found in the age group of 5-10 years (22% of the total number of infected children) (P=0.0025). Cough, fever, and shortness of breath were common symptoms in hMPV-positive children: 15(83.3%), 13(72.2%), and 12(66.7%), respectively. There was a statistically significant correlation between common clinical symptoms and the age group of hMPV-positive children: symptoms were common in the age group of <1 year. Conclusion: Our study represents the first report in Khartoum, Sudan, on the detection of hMPV using RT-LAMP. RT-LAMP is a valuable, quick diagnostic technique for hMPV detection.

scholarly journals Metapneumovirus infection in children

2020 ◽  
Vol 11 (5) ◽  
pp. 13-19
Author(s):  
Elena V. Sharipova ◽  
Irina V. Babachenko ◽  
Elizaveta D. Orlova
Keyword(s):  
Respiratory Tract ◽  
Lower Respiratory Tract ◽  
Respiratory Tract Infections ◽  
Viral Infections ◽  
Clinical Symptoms ◽  
Hospitalized Children ◽  
Lower Respiratory Tract Infections ◽  
Nucleic Acid Isolation ◽  
Hmpv Infection ◽  
Tract Infections

Objective: to study the clinical features of metapneumovirus infection in children of different ages in a hospital. Materials and methods. A retrospective analysis of medical records of 142 patients aged 1 month to 14 years inclusive who were hospitalized in the period from January 2012 to April 2019. Metapneumovirus infection was confirmed by hMPV nucleic acid isolation by PCR in nasopharyngeal smears. Results. Metapneumovirus infection is detected among hospitalized children with acute respiratory viral infections in 4,4% of cases. In the age structure, 72,2% are children under 4 years old, and the maximum incidence rate is among children aged 3 years of life. The leading clinical symptoms are cough in 93,0% of cases and rhinitis in 96,5% of cases.In 88,2% of children, the disease proceeds with an increase in temperature 38 C, including in 34,6% 39,5 C and above. Symptoms of gastrointestinal dysfunction in the form of vomiting and diarrhea develop in 26,1% and 22,5% of children, respectively. 78,2% of patients requiring hospitalization suffer hMPV infection with damage to the lower respiratory tract, including in the form of bronchitis in 85,6% of cases and pneumonia in 14,4% of cases. The disease is complicated by the development of bronchial obstructive syndrome in 38,7% and acute respiratory failure in 22,3% of cases. ARF and BOS are significantly more likely to develop in children of the first 3 years of life 71,0% versus 29,0% in children of the older age group (p = 0.038) and 69,8% against 30,2% (p = 0.007), respectively. In a clinical blood test for hMPV infection, leukopenia and leukocytosis are detected only in 3,5% and 12,7% of cases, respectively, as well as an increase in ESR in 23,9% of children. The level of CRP in the 93,0% of children was less than 20 mg/l. Conclusions. Virological confirmation of metapneumovirus infection in hospitalized children with lower respiratory tract infections contributes to the formation of an adequate therapeutic tactic.

scholarly journals ACUTE RESPIRATORY TRACT INFECTIONS (ARIS);

2017 ◽  
Vol 24 (02) ◽  
pp. 322-325
Author(s):  
Muhammad Usman Anjum ◽  
Hashim Riaz ◽  
Hafiz Muhammad Tayyab
Keyword(s):  
Respiratory Tract ◽  
Respiratory Tract Infections ◽  
World Health ◽  
Age Group ◽  
Self Medication ◽  
Respiratory Illnesses ◽  
Acute Respiratory Tract Infections ◽  
Predominant Symptom ◽  
Number Of Patients ◽  
Tract Infections

Objective: To determine the epidemiological and clinical profile of patientssuffering from acute respiratory tract infections in our area. Study design: Descriptive study,Setting: Frontier Medical & Dental College, Abbottabad, Pakistan. Period: July to December,2014. Materials and methods: All those children who were less than five years of age andclinically diagnosed with acute respiratory tract infections were included in the study. Whereaschildren who were more than five years of age, or suffering from chronic respiratory illnesses,or having congenital disease of respiratory tract were excluded from the study. Structuredproforma was used to record demographic and clinical data. World health organization’s criteriawere used to categorize the cases of ARIs. Results: A total of 229 patients were included inthe study. There were 109 male patients while 120 female patients with male to female ratioof 1:1.18. Maximum number of patients, 43%, was in the age group of 13-24 months followedby 31% in age group of 1-12 months and 15% in the age group of 25-36 months. The majorityof patients presented with shortness of breath as their main symptom followed by sore throatand cough. About 97% of our patients sought treatment from trained health care professionalwhile only 03% resort to self-medication. Conclusion: Acute respiratory tract infections arean important group of diseases in children of less than five years of age. These infectionsmostly affect children between 1-2 years of age. The predominant symptom is shortness ofbreath followed by sore throat and cough. Therefore, any child presenting with these symptomsshould be carefully evaluated for ARIs and their severity using World Health Organization’scriteria. Early diagnosis and treatment will, in turn, improve prognosis and reduce burden onhealth care facilities.

Epidemiological Distinctive Survey Of Children With Acute Respiratory Tract Infections In Some Selected Hospitals Of Katsina Metropolis, Nigeria

2020 ◽  
Vol 6 (2) ◽  
pp. 120-123
Author(s):  
Umar Shittu ◽  
Idris Zainab-L ◽  
Umma Sada ◽  
Bashir Aliyu
Keyword(s):  
Risk Factors ◽  
Air Pollution ◽  
Respiratory Tract ◽  
Indoor Air ◽  
Significant Influence ◽  
Respiratory Tract Infections ◽  
Indoor Air Pollution ◽  
Age Group ◽  
Acute Respiratory Tract Infections ◽  
Tract Infections

Acute respiratory tract infections (ARIs) in children under the age group of five (5) years are serious infections, which prevent the normal breathing function in the child's system. The infection usually begins as a viral infection in which it enters the child's system through the nose to the trachea (windpipe) and down to the lungs. This study aimed to identify the distinctive risk factors associated with the respiratory tract infection in which later it can lead the infection to become acute and find out the easy ways toward preventing the infections. The study was performed within the period of six (6) months during the rainy season between the periods of April to September using the human subjects under age group of five (5) years. The data analysis was done in the Bioconductor R package, statistics p-value with associated B-value were obtained from the distribution of the moderated t-statistic after the adjustment for multiple testing with a significance level of ((? 0.05) using LIMMA method. Pvclust method was also used to generate thousands of bootstrap samples by randomly sampling elements of the data and then compute graphic hierarchical clustering on each bootstrap copy. Distinctive risk factors of (ARTI) were identified, such as malnutrition (MNT), indoor air pollution (IAP), Crowdy and dirty environment (CDE), and parental education (PE) which shows significant influence on the infection. But indoor air pollution (IAP) with the highest level of significant influence to the infections. It is recommended that parents having children should be enlightened by the health personnel experts through different media communication channels and other channels in order to avoid leaving in dirty, crowdy and unventilated environment and to feed from recommended diets with their children.

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