scholarly journals GAMMA RAYS MUTAGENESIS OF ASPERGILLUS NIGER FOR HYPERPRODUCTION OF MUTAROTASE

2012 ◽  
Vol 2 ◽  
pp. 173
Author(s):  
Modasarah Mazhar ◽  
Keyword(s):  
Aspergillus Niger ◽  
Mutant Strain ◽  
Gamma Rays ◽  
Screening Method ◽  
Production Potential ◽  
Maximum Production ◽  
Dose Rates ◽  
Enhanced Production ◽  
Mutant Strains ◽  
Random Screening

Aspergillus niger was subjected to gamma rays mutagenesis at different dose rates (40 to 120 k.Rads). The mutant strains of Aspergillus niger were isolated and selected by random screening method. The selected mutant derived strains were compared with parent type of Aspergillus niger for enhanced production potential of mutarotase. A mutant strain of Aspergillus niger with maximum production potential of mutarotase was finally selected.

scholarly journals EFFECT OF GROUNDNUT CAKE AND SOYA BEANS ON ENHANCED CITRIC ACID PRODUCTION FROM PAWPAW AND ORANGE PEEL BY MUTANTS OF ASPERGILLUS NIGER

2019 ◽  
Vol 17 (1) ◽  
pp. 147-155
Author(s):  
H. T. BALOGUN-ABIOLA ◽  
S. O. KAREEM ◽  
R. B. AFOLABI ◽  
O. A. AKINLOYE
Keyword(s):  
Citric Acid ◽  
Solid State ◽  
Aspergillus Niger ◽  
Mutant Strain ◽  
Fermentation Process ◽  
Carbon Sources ◽  
Orange Peel ◽  
Nitrogen Sources ◽  
Groundnut Cake ◽  
Mutant Strains

This present study was concerned with the biosynthesis of citric acid (CA) with mutant strain of Aspergillus niger using pawpaw and orange peel as substrates by solid state fermentation process. The A. niger strain isolated from spoilt orange was identified, screened for CA production on Czapek-Dox Agar and subjected to mutation by ethidium bromide. The effect of carbon sources, nitrogen sources and substrates were also determined.  Among the mutant strains, A. niger PJ-02 A120 was found to be the best mutant that produced citric acid (65.00±0.58f) after 48 hours in Vogel’s medium. The effects of carbon sources (sucrose and glucose) on CA production from each substrate (orange and pawpaw peel) using mutant A. niger PJ-02 was determined and sucrose, the best carbon source was combined with two the nitrogen sources (groundnut cake and soyabeans) to determine the most suitable supplement for CA production. Groundnut cake enhances the production of citric acid while soyabeans was inhibitory. Citric acid was further produced in pawpaw peel and orange peel medium containing sucrose (5 %) groundnut cake (2 %), methanol (1.5 %) and the mutant strain. The orange peel substrates yielded 112.07g/kg of CA while 107.17g/kg was recorded for pawpaw peel when fermented for 5 days at 30°C. The Production of citric acid with mutant Aspergillus niger proved better with orange peel than pawpaw peel when optimized with alcohol.      

scholarly journals Enhanced production and characterization of a novel -D-glucose:oxygen-1-oxidoreductase by using Aspergillus niger UV-180-C mutant strain

2011 ◽  
Vol 10 (65) ◽  
pp. 14522-14533 ◽  
Author(s):  
Rasul Samreen ◽  
Anjum Zia Muhammad ◽  
Ahmad Sheikh Munir ◽  
Iftikhar Tehreema
Keyword(s):  
Aspergillus Niger ◽  
Mutant Strain ◽  
Enhanced Production

Ovarian Dysfunction in Fetally Irradiated Beagles

1977 ◽  
Vol 35 ◽  
pp. 658-659
Author(s):  
T. M. Seed ◽  
M. H. Sanderson ◽  
D. L. Gutzeit ◽  
T. E. Fritz ◽  
D. V. Tolle ◽  
...  
Keyword(s):  
Gamma Rays ◽  
Low Dose ◽  
Long Term Effects ◽  
Ovarian Dysfunction ◽  
Dose Rates ◽  
Highly Sensitive ◽  
Microscopic Evaluation ◽  
Ovarian Pathology ◽  
Normal Offspring

The developing mammalian fetus is thought to be highly sensitive to ionizing radiation. However, dose, dose-rate relationships are not well established, especially the long term effects of protracted, low-dose exposure. A previous report (1) has indicated that bred beagle bitches exposed to daily doses of 5 to 35 R 60Co gamma rays throughout gestation can produce viable, seemingly normal offspring. Puppies irradiated in utero are distinguishable from controls only by their smaller size, dental abnormalities, and, in adulthood, by their inability to bear young.We report here our preliminary microscopic evaluation of ovarian pathology in young pups continuously irradiated throughout gestation at daily (22 h/day) dose rates of either 0.4, 1.0, 2.5, or 5.0 R/day of gamma rays from an attenuated 60Co source. Pups from non-irradiated bitches served as controls. Experimental animals were evaluated clinically and hematologically (control + 5.0 R/day pups) at regular intervals.

scholarly journals Evaluation Agronomy character of irradiated black rice Cempo Ireng mutant strains MS with 300 Gy of gamma rays

2020 ◽  
Author(s):  
Nandariyah ◽  
Martheffany Devitha P. P. ◽  
Parjanto ◽  
Suharyana ◽  
Riyatun ◽  
...  
Keyword(s):  
Gamma Rays ◽  
Black Rice ◽  
Mutant Strains

Dose Rates from Plane Sources of Gamma Rays

1975 ◽  
Vol 29 (1) ◽  
pp. 131-136 ◽  
Author(s):  
H. W. Dickson ◽  
G. D. Kerr
Keyword(s):  
Gamma Rays ◽  
Dose Rates

scholarly journals Effectiveness of the toxin-degrading strain screening strategy: Deficiency and improvement in screening the gossypol-degrading microorganism Aspergillus niger

2021 ◽  
Author(s):  
Chen Zhang ◽  
Gangqin Shu ◽  
Hui Wang ◽  
Daojie Li ◽  
Yuan Xü ◽  
...  
Keyword(s):  
Aspergillus Niger ◽  
Free Gossypol ◽  
Screening Method ◽  
Screening Strategy ◽  
Gossypol Content ◽  
Control Group ◽  
Total Gossypol ◽  
Screening Strategies ◽  
Free Gossypol Content ◽  
Degrading Microorganism

Abstract The effectiveness of the classic screening strategy was verified, by duplicating and verifying the degradation of gossypol by the Aspergillus niger. It can reduce the free gossypol content through biosorption but has no effect on the total gossypol content and cannot effectively degrade gossypol. And the most interesting thing we found the strain can secrete agarase, utilise agar as carbon source. In this case, that will mislead researchers and lead them to make wrong judgments. That turns out the usual methods of previous screening strategies are not rigorous enough, the classic screening method has defect in screening toxin-degrading strain, so agar control group should be added. In this study, some suggestions are put forward to optimise the same type of experiments and broaden the idea of detoxification by microorganisms and provide reference for screening effective toxin-degrading microorganisms.

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