scholarly journals AB115. Effects of target gene expression on ex vivo differentiated mesenchymal stromal cells transfected by lentiviral vector with PDE5 of short hairpin RNA

2016 ◽  
Vol 5 (S1) ◽  
pp. AB115-AB115
Author(s):  
Heng-Jun Xiao ◽  
Weixin Yan ◽  
Jun Chen ◽  
Xin Gao ◽  
Li Zhuan ◽  
...  
Keyword(s):  
Gene Expression ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Target Gene ◽  
Lentiviral Vector ◽  
Ex Vivo ◽  
Short Hairpin Rna ◽  
Hairpin Rna ◽  
Target Gene Expression ◽  
Short Hairpin

scholarly journals Lentiviral Vector-Mediated Delivery of Short Hairpin RNA Results in Persistent Knockdown of Gene Expression in Mouse Brain

2003 ◽  
Vol 14 (18) ◽  
pp. 1799-1807 ◽  
Author(s):  
Chris Van den Haute ◽  
Kristel Eggermont ◽  
Bart Nuttin ◽  
Zeger Debyser ◽  
Veerle Baekelandt
Keyword(s):  
Gene Expression ◽  
Mouse Brain ◽  
Lentiviral Vector ◽  
Short Hairpin Rna ◽  
Hairpin Rna ◽  
Short Hairpin

scholarly journals Ex Vivo Rat Transected Spinal Cord Slices as a Model to Assess Lentiviral Vector Delivery of Neurotrophin-3 and Short Hairpin RNA against NG2

Biology ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 54 ◽  
Author(s):  
Azim Patar ◽  
Peter Dockery ◽  
Siobhan McMahon ◽  
Linda Howard
Keyword(s):  
Spinal Cord ◽  
Lentiviral Vector ◽  
Ex Vivo ◽  
Axonal Growth ◽  
Short Hairpin Rna ◽  
Slice Culture ◽  
Hairpin Rna ◽  
Neurotrophin 3 ◽  
Short Hairpin

The failure of the spinal cord to regenerate can be attributed both to a lack of trophic support for regenerating axons and to upregulation of inhibitory factors such as chondroitin sulphate proteoglycans including NG2 following injury. Lentiviral vector-mediated gene therapy is a possible strategy for treating spinal cord injury (SCI). This study investigated the effect of lentiviral vectors expressing Neurotrophin-3 (NT-3) and short-hairpin RNA against NG2 (NG2 sh) to enhance neurite outgrowth in in vitro and ex vivo transection injury models. Conditioned medium from cells transduced with NT-3 or shNG2 lentiviruses caused a significant increase in neurite length of primary dorsal root ganglia neurons compared to the control group in vitro. In an ex vivo organotypic slice culture (OSC) transduction with Lenti-NT-3 promoted axonal growth. Transducing OSCs with a combination of Lenti-NT-3/NG2 sh lead to a further increase in axonal growth but only in injured slices and only within the region adjacent to the site of injury. These findings suggest that the combination of lentiviral NT-3 and NG2 sh reduced NG2 levels and provided a more favourable microenvironment for neuronal regeneration after SCI. This study also shows that OSCs may be a useful platform for studying glial scarring and potential SCI treatments.

Lentiviral vector delivery of short hairpin RNA to NG2 and neurotrophin-3 promotes locomotor recovery in injured rat spinal cord

Cytotherapy ◽  
2012 ◽  
Vol 14 (10) ◽  
pp. 1235-1244 ◽  
Author(s):  
Eleanor M. Donnelly ◽  
Nicolas N. Madigan ◽  
Gemma E. Rooney ◽  
Andrew Knight ◽  
Bingkun Chen ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Lentiviral Vector ◽  
Short Hairpin Rna ◽  
Rat Spinal Cord ◽  
Hairpin Rna ◽  
Locomotor Recovery ◽  
Neurotrophin 3 ◽  
Short Hairpin ◽  
Vector Delivery

scholarly journals Clearance of Measles Virus from Persistently Infected Cells by Short Hairpin RNA

2009 ◽  
Vol 83 (18) ◽  
pp. 9423-9431 ◽  
Author(s):  
Michael Zinke ◽  
Sabine Kendl ◽  
Katrin Singethan ◽  
Markus Fehrholz ◽  
Dajana Reuter ◽  
...  
Keyword(s):  
Measles Virus ◽  
Fluorescent Protein ◽  
Lentiviral Vector ◽  
Test System ◽  
Short Hairpin Rna ◽  
Hairpin Rna ◽  
Persistently Infected ◽  
Infected Cells ◽  
Short Hairpin ◽  
Nt2 Cells

ABSTRACT Subacute sclerosing panencephalitis (SSPE) is a demyelinating central nervous system disease caused by a persistent measles virus (MV) infection of neurons and glial cells. There is still no specific therapy available, and in spite of an intact innate and adaptive immune response, SSPE leads inevitably to death. In order to select effective antiviral short interfering RNAs (siRNAs), we established a plasmid-based test system expressing the mRNA of DsRed2 fused with mRNA sequences of single viral genes, to which certain siRNAs were directed. siRNA sequences were expressed as short hairpin RNA (shRNA) from a lentiviral vector additionally expressing enhanced green fluorescent protein (EGFP) as an indicator. Evaluation by flow cytometry of the dual-color system (DsRed and EGFP) allowed us to find optimal shRNA sequences. Using the most active shRNA constructs, we transduced persistently infected human NT2 cells expressing virus-encoded HcRed (piNT2-HcRed) as an indicator of infection. shRNA against N, P, and L mRNAs of MV led to a reduction of the infection below detectable levels in a high percentage of transduced piNT2-HcRed cells within 1 week. The fraction of virus-negative cells in these cultures was constant over at least 3 weeks posttransduction in the presence of a fusion-inhibiting peptide (Z-Phe-Phe-Gly), preventing the cell fusion of potentially cured cells with persistently infected cells. Transduced piNT2 cells that lost HcRed did not fuse with underlying Vero/hSLAM cells, indicating that these cells do not express viral proteins any more and are “cured.” This demonstrates in tissue culture that NT2 cells persistently infected with MV can be cured by the transduction of lentiviral vectors mediating the long-lasting expression of anti-MV shRNA.

Comparison of approaches for efficient gene silencing induced by microRNA-based short hairpin RNA and indicator gene expression

2009 ◽  
Vol 37 (4) ◽  
pp. 1831-1839 ◽  
Author(s):  
Z. X. Shan ◽  
Q. X. Lin ◽  
C. Y. Deng ◽  
Z. L. Zhou ◽  
H. H. Tan ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Silencing ◽  
Short Hairpin Rna ◽  
Hairpin Rna ◽  
Efficient Gene ◽  
Short Hairpin
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