scholarly journals Stable Suppression of Gene Expression in Murine Embryonic Stem Cells by RNAi Directed from DNA Vector-Based Short Hairpin RNA

Stem Cells ◽  
2004 ◽  
Vol 22 (1) ◽  
pp. 93-99 ◽  
Author(s):  
Fu-Chou Tang ◽  
Guo-Liang Meng ◽  
Hong-Bo Yang ◽  
Cheng-Jian Li ◽  
Yan Shi ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Short Hairpin Rna ◽  
Hairpin Rna ◽  
Murine Embryonic Stem Cells ◽  
Short Hairpin ◽  
Dna Vector ◽  
Suppression Of Gene Expression

scholarly journals Specific Knockdown of OCT4 in Human Embryonic Stem Cells by Inducible Short Hairpin RNA Interference

Stem Cells ◽  
2009 ◽  
Vol 27 (4) ◽  
pp. 776-782 ◽  
Author(s):  
Gaetano Zafarana ◽  
Stuart R. Avery ◽  
Katie Avery ◽  
Harry D. Moore ◽  
Peter W. Andrews
Keyword(s):  
Stem Cells ◽  
Rna Interference ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Short Hairpin Rna ◽  
Hairpin Rna ◽  
Short Hairpin ◽  
Short Hairpin Rna Interference

scholarly journals Functional impact of cancer-associated cohesin variants on gene expression and cellular identity

Genetics ◽  
2021 ◽  
Author(s):  
Natalie L Rittenhouse ◽  
Zachary M Carico ◽  
Ying Frances Liu ◽  
Holden C Stefan ◽  
Nicole L Arruda ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Chromosome Structure ◽  
Embryonic Stem ◽  
Sequence Variants ◽  
Dna Loops ◽  
Murine Embryonic Stem Cells ◽  
Recurrent Mutations ◽  
Cellular Identity

Abstract Cohesin is a ring-shaped protein complex that controls dynamic chromosome structure. Cohesin activity is important for a variety of biological processes, including formation of DNA loops that regulate gene expression. The precise mechanisms by which cohesin shapes local chromosome structure and gene expression are not fully understood. Recurrent mutations in cohesin complex members have been reported in various cancers, though it is not clear whether many cohesin sequence variants have phenotypes and contribute to disease. Here, we utilized CRISPR/Cas9 genome editing to introduce a variety of cohesin sequence variants into murine embryonic stem cells and investigate their molecular and cellular consequences. Some of the cohesin variants tested caused changes to transcription, including altered expression of gene encoding lineage-specifying developmental regulators. Altered gene expression was also observed at insulated neighborhoods, where cohesin-mediated DNA loops constrain potential interactions between genes and enhancers. Furthermore, some cohesin variants altered the proliferation rate and differentiation potential of murine embryonic stem cells. This study provides a functional comparison of cohesin variants found in cancer within an isogenic system, revealing the relative roles of various cohesin perturbations on gene expression and maintenance of cellular identity.

scholarly journals Changes of HCN gene expression and If currents in Nkx2.5-positive cardiomyocytes derived from murine embryonic stem cells during differentiation

2008 ◽  
Vol 29 (4) ◽  
pp. 195-203 ◽  
Author(s):  
Shuichi Yano ◽  
Junichiro Miake ◽  
Einosuke Mizuta ◽  
Kasumi Manabe ◽  
Udin Bahrudin ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Murine Embryonic Stem Cells

BMP4 Favors Hematopoietic Differentiation from Murine Embryonic Stem Cells and Acts by Activation of the cdx-hox Pathway.

Blood ◽  
2005 ◽  
Vol 106 (11) ◽  
pp. 3613-3613
Author(s):  
Claudia Lengerke ◽  
Yuan Wang ◽  
Frank Yates ◽  
Leila Maouche-Chretien ◽  
George Q. Daley
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Hematopoietic Stem Cells ◽  
Hox Genes ◽  
Embryonic Stem ◽  
Embryoid Bodies ◽  
Hematopoietic Stem ◽  
Hox Gene ◽  
Murine Embryonic Stem Cells

Abstract Cdx4 and cdx1, members of the caudal family of homeodomain-containing transcriptional regulators, are important for specifying the hematopoietic fate of mesoderm in the zebrafish. We have shown that the cdx4 gene plays a role in enhancing hematopoietic fate during in vitro differentiation of murine ESCs (Davidson et al., Nature 2003). Cdx4 induces hox genes, and genetic modification of mESCs with a combination of cdx4 and hoxb4 promotes long-term engraftment of ESC-derived HSCs in lethally irradiated primary and secondary mice (Wang et al, submitted). While cdx1 is known to be a direct target of signaling by the embryonic morphogens fgf, wnt3a, and retinoids, morphogens acting upstream of cdx4 have not yet been defined. Our goal is to determine optimal morphogen conditions for hematopoietic commitment from murine embryonic stem cells by evaluating activation of the cdx-hox pathway. We have developed quantitative RT-PCR assays for the cdx genes (cdx4, cdx1 and cdx2) and multiple hox genes as well as markers specific to hematopoietic stem cells and lineages. We have used these assays, together with a reporter line engineered to express GFP from the brachury locus (Fehling et al., Development 2003), to characterize the conditions for mesodermal induction and hematopoietic fate specification following addition of morphogens to differentiating cultures of ES cells under serum-free conditions. Among all morphogens tested (BMP4, activin, nodal, wnt3a, wnt5a, sonic hedgehog, indian hedgehog, retinoic acid), only BMP4 has been found to strongly induce CDX4 gene expression within the developing embryoid bodies, while addition of the BMP4 inhibitor noggin to serum suppressed CDX4 expression. Addition of BMP4 significantly increases the number of emerging CD41+ and CD45+ cells, the precursors of definitive hematopoietic stem cells. We are currently analyzing the functional changes following BMP4 exposure, and correlating hematopoietic maturation with changes in the Hox gene expression pattern. Analysis of the cdx-hox gene pathway provides a means of otpimizing induction of hematopoietic fate by application of embryonic morphogens.

scholarly journals Mapping Dynamic Histone Acetylation Patterns to Gene Expression in Nanog-Depleted Murine Embryonic Stem Cells

2010 ◽  
Vol 6 (12) ◽  
pp. e1001034 ◽  
Author(s):  
Florian Markowetz ◽  
Klaas W. Mulder ◽  
Edoardo M. Airoldi ◽  
Ihor R. Lemischka ◽  
Olga G. Troyanskaya
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Histone Acetylation ◽  
Embryonic Stem ◽  
Murine Embryonic Stem Cells

scholarly journals Cell signaling coordinates global Polycomb Repressive Complex 2 recruitment and gene expression in murine embryonic stem cells

2019 ◽  
Author(s):  
Mohammad B. Aljazi ◽  
Yuen Gao ◽  
Yan Wu ◽  
George I. Mias ◽  
Jin He
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Transcription Factors ◽  
Embryonic Stem Cells ◽  
Cell Signaling ◽  
Embryonic Stem ◽  
Erk Signaling ◽  
Gene Promoters ◽  
Polycomb Repressive Complex 2 ◽  
Murine Embryonic Stem Cells

SummaryThe recruitment of Polycomb repressive complex 2 (PRC2) to gene promoters is critical for its function in repressing gene expression in murine embryonic stem cells (mESCs). However, previous studies have demonstrated although the expression of early lineage-specific genes is largely repressed, the genome-wide PRC2 occupancy is unexpectedly reduced in naïve mESCs. In this study, we provide evidence to show the FGF/ERK signaling determines the global PRC2 occupancy through regulating the expression of PRC2-recruting factor JARID2 in naïve mESCs. At the transcriptional level, the de-repression of bivalent genes is predominantly determined by the presence of cell signaling-associated transcription factors but not the status of PRC2 occupancy at gene promoters. Hence, this study not only reveals a key molecular mechanism by which the FGF/ERK signaling in regulating the PRC2 occupancy in mESCs, but also elucidates a fundamental question regarding the functional roles of transcription factors and Polycomb-mediated epigenetic mechanisms in transcriptional regulation.

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