scholarly journals Continuous monitoring of the liver graft temperature: relationship between bacterial contamination of the perfusion fluid and early outcome

2016 ◽  
Vol 4 (20) ◽  
pp. 397-397 ◽  
Author(s):  
Giovanni Battista Levi Sandri ◽  
Roberto Luca Meniconi ◽  
Marco Colasanti ◽  
Nicola Guglielmo ◽  
Edoardo de Werra ◽  
...  
Keyword(s):  
Continuous Monitoring ◽  
Bacterial Contamination ◽  
Liver Graft ◽  
Temperature Relationship ◽  
Perfusion Fluid ◽  
Early Outcome

scholarly journals AN IMPROVED MICROFLUORIMETER FOR MEASURING BRIGHTNESS OF FLUORESCENT ANTIBODY REACTIONS

1967 ◽  
Vol 15 (1) ◽  
pp. 38-45 ◽  
Author(s):  
MORRIS GOLDMAN
Keyword(s):  
Continuous Monitoring ◽  
Bacterial Contamination ◽  
Fluorescent Antibody ◽  
Standard Errors ◽  
Microscope Slide ◽  
High Reproducibility ◽  
Excitation Beam ◽  
Virus Particles ◽  
Beam Output ◽  
Intermittent Measurements

A microfluorimeter suitable for measuring fluorescence of objects as small as bacteria is described. It consists of a fluorescence microscope equipped with one photomultiplier for intermittent measurements of observed objects, and another for continuous monitoring of the excitation beam. Output of the two photomultipliers is presented on a recorder as a ratio of the two currents. The light source is a high pressure mercury arc operated on de, and the instrument is aligned and standardized with the help of a standard fluorescent crystal mounted on a microscope slide. Measurements of intestinal amebae stained with a refined fraction of fluoresceinlabeled globulin showed high reproducibility on duplicate smears in spite of the presence of gross bacterial contamination. Standard errors of the means obtained from samples of 30 amebae averaged 5.6% of the means. The technique is capable of revealing extremely fine immunological distinctions in antigenic materials like intact microorganisms, virus particles and the like, even in the presence of tissue cells and other "contaminant" debris.

scholarly journals Clinical impact of bacterial contamination of perfusion fluid in kidney transplantation

SpringerPlus ◽  
2016 ◽  
Vol 5 (1) ◽  
Author(s):  
A. Ranghino ◽  
D. Diena ◽  
F. Simonato ◽  
M. Messina ◽  
M. Burdese ◽  
...  
Keyword(s):  
Kidney Transplantation ◽  
Bacterial Contamination ◽  
Clinical Impact ◽  
Perfusion Fluid

Morphological Observations on the Granule Types in Rabbit Extra-Adrenal Chromaffin Cells.

1975 ◽  
Vol 33 ◽  
pp. 318-319
Author(s):  
Joe A. Mascorro ◽  
Robert D. Yates
Keyword(s):  
Chromaffin Cells ◽  
Sodium Phosphate ◽  
Ganglion Cells ◽  
Ultrastructural Level ◽  
Osmic Acid ◽  
Adrenal Chromaffin Cells ◽  
Potassium Iodate ◽  
Perfusion Fluid ◽  
New Zealand White Rabbits ◽  
Adrenal Chromaffin

Extra-adrenal chromaffin organs (abdominal paraganglia) constitute rich sources of catecholamines. It is believed that these bodies contain norepinephrine exclusively. However, the present workers recently observed epinephrine type granules in para- ganglion cells. This report investigates catecholamine containing granules in rabbit paraganglia at the ultrastructural level.New Zealand white rabbits (150-170 grams) were anesthetized with 50 mg/kg Nembutal (IP) and perfused with 3% glutaraldehyde buffered with 0.2M sodium phosphate, pH 7.3. The retroperitoneal tissue blocks were removed and placed in perfusion fluid for 4 hours. The abdominal paraganglia were dissected from the blocks, diced, washed in phosphate buffer and fixed in 1% osmic acid buffered with phosphate. In other animals, the glutaraldehyde perfused tissue blocks were immersed for 1 hour in 3% glutaraldehyde/2.5% potassium iodate buffered as before. The paraganglia were then diced, separated into two vials and washed in the buffer. A portion of this tissue received osmic acid fixation.

The multicompartment SlideReactor – a novel concept for continuous monitoring of co-cultured cells

2009 ◽  
Vol 47 (01) ◽  
Author(s):  
N Billecke ◽  
S Tröller ◽  
N Raschzok ◽  
MH Morgül ◽  
NN Kammer ◽  
...  
Keyword(s):  
Continuous Monitoring ◽  
Cultured Cells ◽  
Novel Concept
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