3D 6DOF Manipulation of Microbead by Laser Tweezers

2006 ◽  
Vol 18 (2) ◽  
pp. 153-159 ◽  
Author(s):  
Fumihito Arai ◽  
◽  
Toshiaki Endo ◽  
Ryuji Yamauchi ◽  
Toshio Fukuda ◽  
...  

Laser tweezers are suitable for manipulation of a single microscopic biological object. It can manipulate micro bio-object by noncontact in closed space. Single cell manipulation is important for biological research works, and 3D 6DOF manipulation (Position control and Orientation control) is useful technique in many biological experiments. Here we proposed 3D synchronized laser manipulation system by which we can manipulate multiple micro-objects along each designed trajectory in 3D space. Position and orientation of microbeads can be controlled by the newly developed 3D synchronized laser micromanipulation system. We succeeded in the orientation control of the microbead by using the laser trapped microtools. We demonstrate 3D 6DOF manipulation of the microbead by the experiment.

2005 ◽  
Vol 17 (3) ◽  
pp. 335-341 ◽  
Author(s):  
Hisataka Maruyama ◽  
◽  
Fumihito Arai ◽  
Toshio Fukuda

Single cell experiments have become very important for investigating unknown cell properties. We developed a novel technique to study individual cell properties on a chip using newly developed cell manipulation by laser tweezers with the photo-crosslinkable resin, using this resin to developed functional colored, fluorescent, cell binding, rotation free, and rope shaped microtools on a chip. Colored and fluorescent microtools are for cell manipulation using inexpensive image processing. Cell binding microtools are for high-speed transport of target cells. Rotation free microtools are for attitude control and precise force measurement of cells and DNA. Rope shaped microtools are for versatile manipulation. Laser tweezers is used to position-control microtools. We used a mercury lamp for UV illumination at the local area for combining microtools and fabricated our functional microtools and manipulated cells on the microchip.


2021 ◽  
Author(s):  
Eko Henfri Binugroho ◽  
Andri Setiawan ◽  
Yudha Sadewa ◽  
Prishandy Hamami Amrulloh ◽  
Kafin Paramasastra ◽  
...  

2007 ◽  
Vol 19 (2) ◽  
pp. 198-204 ◽  
Author(s):  
Fumihito Arai ◽  
◽  
Toshiaki Endo ◽  
Ryuji Yamauchi ◽  
Toshio Fukuda ◽  
...  

We developed a novel technique for manipulating lipid nanotubes in 3D space by using laser tweezers in water solutions. Laser tweezers are well known for their use in noncontact manipulation in a closed space. We reported on 3D 6DOF manipulation of microbeads using 3D synchronized laser micromanipulation (SLM), but blur is generated in the microscope image when multiple points at different heights are trapped by scanning the focal point of the laser. It is difficult to manipulate a nanoscale rod-like object stably in 3D space, since the optical trap force is weak. To manipulate a lipid nanotube, for example, we developed an observation system to less image blur by synchronizing the shutter timing of the CCD camera and laser scanning. We thus obtained a clear image of targets at different heights while manipulating them. We then developed functional gel microtools that adhere to lipid nanotubes and succeeded in controlling the position and orientation of lipid nanotubes by using 3D SLM with shutter timing control and novel functional gel microtools.


Author(s):  
Deepak Parajuli ◽  
Mark D. Bedillion ◽  
Randy C. Hoover

An actuator array is a planar distributed manipulation system that uses multiple two degree-of-freedom actuators to manipulate objects with three degrees of freedom (x, y and θ). This paper presents an accurate method of estimating position and orientation of an object using local sensing and communication. In this method, each of the distributed modules contains a number of binary sensors, weight sensors, and two planar actuators. The binary sensors combined together give a binary image and analog sensors in each module combined together form a grayscale image representation of the weight distribution of the object under manipulation. Additive normalization has been used to combine binary and grayscale distributed sensing images together to come up with increased precision estimates of the position and orientation of an object. A distributed sensing simulation has been developed in Simulink and the effectiveness of this method has been verified for rectangular and circular objects using the Simulink model.


2001 ◽  
Vol 44 (1) ◽  
pp. 188-195 ◽  
Author(s):  
Hiroaki OZAKI ◽  
Takahiro OHGUSHI ◽  
Tetsuji SHIMOGAWA ◽  
Chang-jun LIN

2014 ◽  
Vol 625 ◽  
pp. 717-721 ◽  
Author(s):  
Qin Zhang ◽  
Jian Hua Wang ◽  
Wei Jun Huang ◽  
Hisayuki Aoyama

The adjustment of cell position and orientation is important in cell micromanipulation of bioengineering, which directly affects the efficiency and success ratio of the micromanipulation. Cell orientation can be adjusted by swirl, which comes from a pair of parallel opposite micro-fluids from two tiny tubes, and the cell in swirl center will rotate. Quantitative control of rotation angle and velocity of the cell can be achieved by adjusting the velocity and frequency of micro-fluid. This control method of cell orientation has prospective applications for its non-contact characteristics. However, cell will not be right in the swirl center in practical operations. And if the eccentric cell can rotate steadily, swirl method will be more reliable. So numerical simulation was conducted to study the movement trajectory of cells with eccentricity, and the influences of eccentric directions and eccentric distances were discussed. The simulation results indicate the feasibility of orientation control of cells with eccentricity by swirl.


2012 ◽  
Vol 12 (10) ◽  
pp. 3075-3081 ◽  
Author(s):  
Tobias Beutel ◽  
Nelson Ferreira ◽  
Anne Balck ◽  
Monika Leester-Schadel ◽  
Stephanus Buttgenbach

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