LC-MS/MS based targeted metabolomics method for analysis of serum and cerebrospinal fluid

2019 ◽  
Vol 88 (1) ◽  
pp. 12-20
Author(s):  
Szymon Plewa ◽  
Paweł Dereziński ◽  
Jolanta Florczak-Wyspiańska ◽  
Karolina Popławska-Domaszewicz ◽  
Wojciech Kozubski ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
High Performance ◽  
Ion Source ◽  
Low Molecular Weight ◽  
Successful Implementation ◽  
Tandem Mass ◽  
Triple Quadrupole ◽  
Targeted Metabolomics ◽  
The Mean ◽  
Liquid Chromatography System

Introduction. Recent instrumentation and software advancement enabled to develop new, high‑throughput targeted metabolomics methods for in‑depth exploration of metabolome in a quantitative manner.Material and Methods. The presented targeted metabolomics approach allows to analyze both of serum and CSF in the same way, with identical sample preparation procedures. The analyses were carried out using high‑performance liquid chromatography system coupled to triple quadrupole tandem mass spectrometer with electrospray ion source (LC‑ESI‑QqQ‑MS/MS). Results. The applied targeted metabolomics approach enabled to determine a wide panel of metabolites from different chemical classes of compounds including: acylcarnitines, amino acids and biogenic amines, glycerophospholipids, sphingolipids and sum of hexoses. Finally, 148 metabolites in serum and 57 in cerebrospinal fluid were determined.Conclusions. Here we presented the results of successful implementation of the method of analysis of low‑molecular weight compounds in human serum and CSF using targeted metabolomics. The evaluation of selected groups of metabolites resulted in obtaining the mean concentrations of panel of metabolites in serum and CSF, which gives a valuable information about the metabolome of these matrices.

Evaluation of ozonation as a method for mycotoxins degradation in malting wheat grains

2016 ◽  
Vol 9 (3) ◽  
pp. 409-417 ◽  
Author(s):  
I. Reinholds ◽  
G. Juodeikiene ◽  
E. Bartkiene ◽  
D. Zadeike ◽  
V. Bartkevics ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Performance ◽  
Degradation Rate ◽  
High Resolution Mass Spectrometry ◽  
Tandem Mass ◽  
Triple Quadrupole ◽  
Wheat Grain ◽  
Fusarium Mycotoxins ◽  
Wheat Grains ◽  
The Eu

The influence of ozone (O3) gas on reducing the contamination with Fusarium mycotoxins in malting wheat grains was investigated. Ultra-high performance liquid chromatography coupled to triple quadrupole tandem mass spectrometry (UHPLC-QqQ-MS/MS) and Orbitrap high resolution mass spectrometry (UHPLC-Orbitrap-HRMS) were used to determine mycotoxins in wheat grains before and 40 to 130 min after the exposure to 20 mg/l O3. Pearson’s analysis (R2=0.96-0.98) showed a good correlation between the performance efficiency of both mass spectrometry quantification techniques. The concentrations of determined mycotoxins (zearalenone (ZEA): 19.5-459 µg/kg, deoxynivalenol (DON): 3,370-4,620 µg/kg, T-2 toxin: 19.5-35.4 µg/kg, and HT-2 toxin: 258-819 µg/kg) decreased notably, depending on the duration of contact with ozone. A notable elimination of ZEA, HT-2, and T-2 in wheat grain was observed: the content of these compounds was reduced on average by 58.6, 64.6, and 62%, respectively, already after 40 min of ozonation. The effect was less pronounced in the case of DON, for which the average degradation rate reached the maximum of only 25% after 130 min exposure. We conclude that ozonation for up to 130 min was effective for reducing the content of most mycotoxins determined in this study, except for DON, in contaminated grains to concentrations below the acceptable maximum levels in wheat in accordance to the EU regulations.

scholarly journals LC-MS/MS METHOD FOR THE SIMULTANEOUS DETERMINATION OF CEFEPIME AND TAZOBACTAM IN DOG PLASMA

2018 ◽  
Vol 2 (1) ◽  
pp. 36-45
Author(s):  
Kiran R. Patil ◽  
Sukanta K. Naik ◽  
Vineet S. Zope ◽  
Rajesh P. Chavan ◽  
Ravindra D. Yeole
Keyword(s):  
High Performance ◽  
Kinetic Studies ◽  
Hplc Method ◽  
Tandem Mass ◽  
Isocratic Elution ◽  
Simultaneous Quantification ◽  
Dog Plasma ◽  
Tandem Mass Spectrometric ◽  
The Mean

A high performance liquid chromatography (HPLC) method with tandem mass spectrometric detection (MS/MS) has been developed and validated for the simultaneous quantification of cefepime and tazobactam in dog plasma. The method was developed on amide column with isocratic elution. The developed method is simple and economic in terms of sample preparation. The method is specific, sensitive, accurate, precise and robust. The method was successfully applied for pre-clinical pharmaco-kinetic studies in dogs. The Tmax was found to be 0.5 hr, the mean Cmax and AUC(0-12) displayed dose proportionate response.

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