scholarly journals ISOLASI DAN UJI SITOTOKSIK SENYAWA LIMONEN DARI KULIT BATANG KASTURI (Mangifera casturi)

BIOSCIENTIAE ◽  
2018 ◽  
Vol 8 (2) ◽  
Author(s):  
Kholifatu Rosyidah
Keyword(s):  
Gas Chromatography ◽  
Liquid Chromatography ◽  
Cancer Cell ◽  
Mass Spectroscopy ◽  
Brine Shrimp ◽  
Methylene Chloride ◽  
Cell Leukemia ◽  
Pure Compounds ◽  
Vacuum Liquid Chromatography ◽  
Gas Chromatography Mass Spectroscopy

The air-stem barks of M.casturi were extracted with Methylene Chloride (MTC). The MTC extract’s had treatment with Brine Shrimp Lethality Test (BSLT) than analyzed with probit and resulted LC50= 47,31 ppm. MTC extract’s was fractionated by Vacuum Liquid Chromatography and recrystalization obtained pure compounds. The compounds was analyzed with GC-MS (Gas chromatography-Mass Spectroscopy) and cytotoxic test with cancer cell Leukemia P388. This compound is limonene and have IC50=70 ppm.

Flavor Composition of Maryland Tobacco

1984 ◽  
Vol 12 (5) ◽  
Author(s):  
Joseph N. Schumacher
Keyword(s):  
Gas Chromatography ◽  
Liquid Chromatography ◽  
Capillary Gas Chromatography ◽  
Methylene Chloride ◽  
Minor Component ◽  
The Other ◽  
Glass Capillary ◽  
Preparative Liquid Chromatography ◽  
Gas Chromatography Mass Spectroscopy ◽  
Trace Components

AbstractThis study was undertaken to determine if Maryland tobacco contains any unique flavour components and to compare its composition to that of flue-cured and Burley tobaccos. Methylene chloride extracts prepared from these tobaccos were fractionated by liquid-liquid partitions and by preparative liquid chromatography. The resulting fractions were analysed by glass capillary gas chromatography and by glass capillary gas chromatography / mass spectroscopy. The essential oils of these tobaccos were also examined. Only one minor component was found in Maryland tobacco that was not found in the other tobaccos. If the minor and trace components were further studied, other differences might be found. Based on this study, however, the uniqueness of Maryland tobacco is primarily, if not entirely, due to quantitative differences. Also, Maryland tobacco is no more similar to Burley (middle-stalk or low-stalk leaves) than it is to flue-cured tobacco.

ANTIOXIDANTS FOR EPDM SEALS EXPOSED TO CHLORINATED TAP WATER

2014 ◽  
Vol 87 (1) ◽  
pp. 1-9 ◽  
Author(s):  
Mio Gonokami ◽  
Yoshimasa Yamamoto ◽  
Oraphin Chaikumpollert ◽  
Yoshito Ohtake ◽  
Seiichi Kawahara
Keyword(s):  
Gas Chromatography ◽  
Weight Loss ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Mass Spectroscopy ◽  
High Performance ◽  
Tap Water ◽  
Accelerated Degradation ◽  
Poly Ethylene ◽  
Gas Chromatography Mass Spectroscopy

ABSTRACT A suitable antioxidant for a poly(ethylene-co-propylene-co-5-ethylidene-2-norbornene) (EPDM) seal in tap water applications was determined with respect to volatilization and decomposition of the antioxidants. Seals were prepared by mixing EPDM with 1 phr antioxidant and other ingredients followed by vulcanizing the mixture at 433 K for 20 min. The resulting EPDM seals were immersed into chlorinated water to investigate accelerated degradation. The change in antioxidant content was measured by gas chromatography/mass spectroscopy (GC/MS) and high-performance liquid chromatography (HPLC). The weight loss of amine antioxidants during vulcanization was quite low due to their low volatility and decomposition. Antioxidant weight loss during accelerated degradation depended on both the antioxidant's ability to trap radicals and solubility in chlorinated water.

scholarly journals IDENTIFIKASI SENYAWA METABOLIT SEKUNDER PADA HASIL FRAKSINASI EKSTRAK Phaseolus vulgaris L. DENGAN METODE GAS CHROMATOGRAPHY-MASS SPECTROSCOPY (GC-MS)

2017 ◽  
Vol 3 (1) ◽  
Author(s):  
Khurriyatul Khair ◽  
Yayuk Andayani ◽  
Aliefman Hakim
Keyword(s):  
Gas Chromatography ◽  
Ethyl Acetate ◽  
Mass Spectroscopy ◽  
Polar Fraction ◽  
Green Bean ◽  
Solvent Fractionation ◽  
Vacuum Liquid Chromatography ◽  
Gas Chromatography Mass Spectroscopy ◽  
Tlc Analysis ◽  
Percent Area

This research was aimed to identify some classes of secondary metabolite compound on the fractionation results of green bean extract (P. vulgaris L.) using Gas Chromatography-Mass Spectroscopy (GC-MS) method. Green bean was extracted by methanol solvent. Fractionation of methanol extract used Vacuum Liquid Chromatography (VLC) with some variation of eluents such as n-hexane 100%; n-hexane: ethyl acetate (9 : 1 to 1 : 9); and ethyl acetate 100%, produced 11 major fractions.  Based on Thin Layer Chromatography (TLC) analysis using DCM: MeOH (9.5: 0.5) as mobile phases, these 11 major fractions were classified based on their polarity such as non-polar, semi-polar and polar fraction. The results of identification by GC-MS spectrometer showed the presence of secondary metabolites such as monoterpenes in non-polar fraction; in semi-polar fraction was found terpenoids and steroids; in polar fraction was found monoterpenes, phenolic and steroid as the lowest percent area, that is less than 2%. Keyword: P.vulgaris L extract, fractionation, VLC, GC-MS

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