scholarly journals Effet immunostimulant de quatre additifs alimentaires contre les maladies de Gumboro et de Newcastle chez le poulet de chair (Gallus gallus)

2016 ◽  
Vol 68 (4) ◽  
pp. 185
Author(s):  
Embarek Azeroual ◽  
Abdelhalem Mesfioui ◽  
Khalid Bouzoubaa ◽  
Bouchra Benazzouz ◽  
Aboubaker El Hessni ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Newcastle Disease ◽  
Gallus Gallus ◽  
Test Elisa ◽  
Bursal Disease ◽  
Système Immunitaire ◽  
Type Chair

Le renforcement du système immunitaire chez les espèces aviaires vulnérables est devenu une approche fortement recherchée pour les protéger contre les infections, surtout d’origine virale. La présente étude a eu pour objectif d’évaluer l’impact de quatre additifs alimentaires sur la modulation du système immunitaire, suite à des prises vaccinales contre la maladie de Gumboro [infec­tious bursal disease (IBD)] et de Newcastle [Newcastle disease (ND)] chez le poulet de chair (Gallus gallus). Le suivi expérimental a été fait sur 500 poussins d’un jour de type chair et de souche Ross, également répartis en cinq groupes (quatre traitements et un témoin). Les produits de traitements ont été à base d’antibiotiques, de phytobiotiques (cannelle et gingembre) et de probiotique (levure Saccharomyces cerevisiae), administrés par voie buccale et par l’eau de boisson. Les traitements ont été effectués en trois périodes d’âges différents : au jour (J) J6–J9, J21–J23 et J33–J35. Les doses primo vaccinales ont été adminis­trées simultanément au J7, et les rappels aux J14 et J21 respectivement contre IBD et ND. Des échantillons sériques ont été prélevés chez cinq poulets de chaque parquet aux J18 et J31. Les titres d’anticorps ont été analysés par le test Elisa pour le virus de l’IBD et par le test d’inhibition de l’hémagglutination pour le virus de la ND. Les résultats obtenus ont révélé qu’aucun effet immunostimu­lant vis-à-vis des titres d’anticorps contre le virus d’IBD n’était induit par l’ajout d’un des quatre adjuvants testés (p > 0,05). En revanche pour la ND, un effet immunostimulant a été observé, plus particulièrement chez les groupes traités au gingembre ou à la levure. Cette différence n’a cependant pas été significative par rapport au témoin (p > 0,05).

APPLICATION OF SHIFTING ASSAY OF STANDARDIZED INDIRECT AGGLUTINATION (SSIA) FOR DETECTION OF ANTIGENS OF NEWCASTLE DISEASE AND INFECTIOUS BURSAL DISEASE VIRUSES IN CHICKEN FAECES

2013 ◽  
Vol 83 (5) ◽  
Author(s):  
Phạm Hồng Sơn ◽  
Phạm Hồng Kỳ ◽  
Nguyễn Thị Lan Hương ◽  
Phạm Thị Hồng Hà
Keyword(s):  
Newcastle Disease ◽  
Winter Season ◽  
Summer Season ◽  
Infectious Bursal Disease ◽  
Infection Rates ◽  
Faecal Samples ◽  
Infectious Bursal Disease Viruses ◽  
Bursal Disease ◽  
Long Time ◽  
Higher Sensitivity

. Using the method of shifting assay of standardized indirect agglutination (SSIA), the prevalence of Newcastle disease viruses (NDV) and infectious bursal disease viruses (IBDV) in chickens reared in several districts of Thua Thien Hue province in the Spring-Summer and Fall-Winter seasons was determined. In the Spring-Summer season of 2011, about 22.3% of the chickens were infected with NDV, in which A Luoi  accounted for the highest percentage of 25% of the infected chickens and Huong Thuy  the lowest  of 18.2%. Meanwhile, 36% of the same chickens were infected with IBDV, with the highest percentage (46.66%) also in A Luoi and the lowest (30.3%) also in Huong Thuy. The intensity of NDV infection in the Spring-Summer season in A Luoi and Phu Vang was highest (GMT = 1.45), and in Huong Thuy lowest (GMT = 1.31). In addition, in the Fall-Winter season, about 46% of the chickens were infected with NDV and 46.3% with IBDV in Huong Thuy and Phu Vang – two neighbouring districts of Hue City, in which NDV was detected in 54.4% of the chickens in Huong Thuy and 33.9% in Phu Vang. In contrast, IBDV was detected in 41.9% and 52.7% of the chickens respectively in the two districts. The infection was not inter-dependent. Methodically, although the differences in the infection rates were insignificant with the accuracy of 95%, faecal samples showed higher sensitivity in SSIA analyses for both cases of NDV and IBDV infection in comparision with mouth exudates. By SSIA method, results could be read clearly with unaided eyes for a long time after the performance, and it was also proven applicable for cases of haemagglutinating viruses if proper treatments for depletion of animal RBCs’ surface agglutinins could be applied.

scholarly journals Prevalence of poultry diseases in Gazipur district of Bangladesh

2016 ◽  
Vol 2 (1) ◽  
pp. 107-112 ◽  
Author(s):  
Md Kamrul Hassan ◽  
Md Humayun Kabir ◽  
Md Abdullah Al Hasan ◽  
Shobnom Sultana ◽  
Md Shohidul Islam Khokon ◽  
...  
Keyword(s):  
Newcastle Disease ◽  
Clinical Signs ◽  
Infectious Bursal Disease ◽  
Viral Diseases ◽  
Necrotic Enteritis ◽  
Age Group ◽  
Bacterial Diseases ◽  
Infectious Bronchitis ◽  
Prevalent Disease ◽  
Bursal Disease

This study was designed with a view to investigate the prevalence of poultry diseases in Gazipur district of Bangladesh. A total of 679 poultry birds (313 layers, 338 broilers and 28 cockrels) either dead or live were brought for diagnosis of diseases at Gazipur Sadar Upazilla Veterinary Hospital. The diseases were diagnosed on the basis of history, clinical signs and post-mortem findings. The diseases encountered in layers were bacterial diseases 52.29% (salmonellosis 38.56%, colibacillosis 6.7%, fowl cholera 4.79% and necrotic enteritis 1.60%), viral diseases 23.95% (avian influenza 2.56%, Newcastle disease 16.61%, infectious bronchitis 3.19% and avian leucosis 0.64%), mycoplasmal disease (mycoplasmosis 14.70%) and protozoal disease (coccidiosis 5.75%). Salmonellosis was most prevalent disease in age group of >20 weeks, while Newcastle disease most common in 8 to 20 weeks of age group. In case of broiler, bacterial diseases 28.99% (salmonellosis 21.30% and colibacillosis 7.69%), viral diseases 53.24% (infectious bursal disease 28.99%, Newcastle disease 8.87% and infectious bronchitis 15.38%), mycoplasmal disease (mycoplasmosis 7.1%) and protozoal disease (coccidiosis 6.5%). In cockrels, the most prevalent disease was colibacillosis 35.71% followed by salmonellosis 28.57%, Newcastle disease 14.28% and mycoplasmosis14.28%. So among the diseases, salmonellosis is most prevalent disease followed by infectious bursal disease and mycoplasmosis in different kinds of poultry of Gazipur district of Bangladesh.Asian J. Med. Biol. Res. March 2016, 2(1): 107-112

scholarly journals STUDY THE EFFECT OF MYCOPLASMA CONTAMINATION OF EGGS USED IN VIRUS TITRATION AND EFFICACY OF SOME LIVE ATTENUATED POULTRY VIRAL VACCINES

2016 ◽  
Vol 10 (1) ◽  
pp. 216 ◽  
Author(s):  
Marwa Fathy ◽  
Mounir M. El-safty ◽  
Jakeen K. El-jakee ◽  
Howaida I. Abd-alla ◽  
Hala Mahmoud
Keyword(s):  
Disease Virus ◽  
Newcastle Disease ◽  
Disease Process ◽  
Mycoplasma Gallisepticum ◽  
Infectious Bursal Disease ◽  
Enzyme Linked Immunosorbent Assay ◽  
Infectious Bronchitis ◽  
Bursal Disease ◽  
Specific Pathogen ◽  
Viral Vaccines

ABSTRACTObjective: The study of Mycoplasma gallisepticum (MG) infection is needed, not only to understand the disease process but also to understand theinterference with the evaluation of some live viral poultry vaccines. This study aims to investigate the titration and potency of some live attenuatedpoultry viral vaccines; Newcastle disease, infectious bronchitis, infectious bursal disease, and Reo in both specific pathogen-free (SPF) embryonatedchicken eggs (ECEs) and chickens.Methods: Titration of live attenuated viral poultry vaccines in ECEs was carried out by dividing the inoculated eggs into four groups; the pre-,simultaneously-, post-, and non-MG contaminated. MG effect on the potency test was carried out using seventeen groups of SPF chickens (25 chicken/group) placed into separate isolators. Each live attenuated viral poultry vaccine was inoculated into 4 groups.Results: The highest titer of these vaccines that appeared in MG pre- contaminated ECEs were 1011, 107.5, 107.9, and 10, respectively. The lowest vaccinetiters that appeared in non-MG contaminated ECEs were 108, 106, 106.8, and 1067.5, respectively. Although the potency of these previous vaccines indicated thatthe highest antibodies titer that appeared in MG pre-infected vaccinated chickens were 7.5 log, 36 enzyme-linked immunosorbent assay unit (EU), and42 EU, respectively; the lowest antibodies titer that appeared in non-MG infected vaccinated chickens were 6.5 log22, 12 EU, 17 EU, and 10 EU, respectively.Conclusion: The present study findings underline the importance of using Mycoplasma -free eggs or chicken for the production of virus vaccines.Keywords: Mycoplasma gallisepticum, Newcastle disease virus, Infectious bronchitis virus, Infectious bursal disease virus, Reo virus, Chicken, Specificpathogen-free eggs.

scholarly journals The Biodegradation Role of Saccharomyces cerevisiae against Harmful Effects of Mycotoxin Contaminated Diets on Broiler Performance, Immunity Status, and Carcass characteristics

Animals ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 238 ◽  
Author(s):  
Muhammad Arif ◽  
Atia Iram ◽  
Muhammad A. K. Bhutta ◽  
Mohammed A. E. Naiel ◽  
Mohamed E. Abd El-Hack ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Wall ◽  
Aflatoxin B1 ◽  
Carcass Traits ◽  
Feeding Trial ◽  
Broiler Performance ◽  
Infectious Bronchitis ◽  
Highest Weight ◽  
Bursal Disease ◽  
Group D

A feeding trial (35 days) was carried out to investigate the effect of Saccharomyces cerevisiae cell wall as a mycotoxin biodegradation agent on the performance, feed efficiency, carcass traits, and immunity response against diseases in broilers fed aflatoxin B1 contaminated diets. For this purpose, 200 one day old broilers were randomly allotted into four groups, each with five replicates (10 birds per replicate). Four starter and finisher experimental rations were formulated by using (A) 0, (B) 1.25, (C) 2.5, and (D) 3.75 g kg−1 of Saccharomyces cerevisiae. Experimental diets were contaminated with aflatoxin B1 (100 ppb kg−1 diet). The experimental chicks were kept under standard managerial conditions, and the vaccination program was followed against infectious bursal disease (IBD), infectious bronchitis (IB), and Newcastle disease (ND) diseases. At the end of the feeding trial, carcass, organ weight, and blood samples were collected randomly to determine the carcass traits and antibody titer against ND and IBD viruses. Throughout the experiment, the addition of 3.75 g kg−1 of the Saccharomyces cerevisiae cell wall (Group-D) in feed resulted in the highest weight gain, final weight, feed intake, and the lowest FCR values followed by C group compared with the other groups. All carcass traits were significantly (p > 0.05) improved by increasing the inclusion levels of Saccharomyces cerevisiae in broiler diets. It could be concluded that the broiler diet supplemented with 2.5 or 3.75 g kg−1 of Saccharomyces cerevisiae as a biodegrading agent resulted in improved growth performance, immunity activity and carcass traits, and supplementation with Saccharomyces cerevisiae at these levels can be used effectively in broiler diets without negatively affecting bird health status.

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