Crosstalk Between Human Monocytic U937 Cells and Gingival Fibroblasts in Coculturally Enhanced Matrix Metalloproteinase-2 Expression

2016 ◽  
Vol 87 (10) ◽  
pp. 1228-1237 ◽  
Author(s):  
Po-Jan Kuo ◽  
Hsiao-Lun Lin ◽  
Chi-Yu Lin ◽  
Yu-Tang Chin ◽  
Hsiao-Pei Tu ◽  
...  
Keyword(s):  
Matrix Metalloproteinase ◽  
Matrix Metalloproteinase 2 ◽  
U937 Cells ◽  
Gingival Fibroblasts

CD147 self‐regulates matrix metalloproteinase‐2 release in gingival fibroblasts after coculturing with U937 monocytic cells

2019 ◽  
Vol 91 (5) ◽  
pp. 651-660
Author(s):  
Tat‐Ming Lai ◽  
Po‐Jan Kuo ◽  
Chi‐Yu Lin ◽  
Yu‐Tang Chin ◽  
Hsiao‐Lun Lin ◽  
...  
Keyword(s):  
Matrix Metalloproteinase ◽  
Matrix Metalloproteinase 2 ◽  
Gingival Fibroblasts ◽  
Monocytic Cells

scholarly journals Effect of Azithromycin on Proinflammatory Cytokine Production in Gingival Fibroblasts and the Remodeling of Periodontal Tissue

2020 ◽  
Vol 10 (1) ◽  
pp. 99
Author(s):  
Takatoshi Nagano ◽  
Takao Yamaguchi ◽  
Sohtaro Kajiyama ◽  
Takuma Suzuki ◽  
Yuji Matsushima ◽  
...  
Keyword(s):  
Gene Expression ◽  
Matrix Metalloproteinase ◽  
Cytokine Production ◽  
Signal Transduction Pathway ◽  
Matrix Metalloproteinase 2 ◽  
Dependent Manner ◽  
Gingival Fibroblasts ◽  
Periodontal Ligament Fibroblasts ◽  
Concentration Dependent Manner ◽  
Matrix Metalloproteinase 1

Previous reports have shown that azithromycin (AZM), a macrolide antibiotic, affects collagen synthesis and cytokine production in human gingival fibroblasts (hGFs). However, there are few reports on the effect of AZM on human periodontal ligament fibroblasts (hPLFs). In the present study, we comparatively examined the effects of AZM on hGFs and hPLFs. We monitored the reaction of AZM under lipopolysaccharide (LPS) stimulation or no stimulation in hGFs and hPLFs. Gene expression analyses of interleukin-6 (IL-6), interleukin-8 (IL-8), matrix metalloproteinase-1 (MMP-1), matrix metalloproteinase-2 (MMP-2), and Type 1 collagen were performed using reverse transcription-polymerase chain reaction (RT-PCR). Subsequently, we performed Western blotting for the analysis of the intracellular signal transduction pathway. In response to LPS stimulation, the gene expression levels of IL-6 and IL-8 in hGFs increased due to AZM in a concentration-dependent manner, and phosphorylation of nuclear factor kappa B (NF-κB) was also promoted. Additionally, AZM caused an increase in MMP-1 expression in hGFs, whereas it did not affect the expression of any of the analyzed genes in hPLFs. Our findings indicate that AZM does not affect hPLFs and acts specifically on hGFs. Thus, AZM may increase the expression of IL-6 and IL-8 under LPS stimulation to modify the inflammatory response and increase the expression of MMP-1 to promote connective tissue remodeling.

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