Peroxisome proliferator-activated receptor δ inhibitsPorphyromonas gingivalislipopolysaccharide-induced activation of matrix metalloproteinase-2 by downregulating NADPH oxidase 4 in human gingival fibroblasts

2015 ◽  
Vol 31 (5) ◽  
pp. 398-409 ◽  
Author(s):  
T. Yoo ◽  
S.A. Ham ◽  
J.S. Hwang ◽  
W.J. Lee ◽  
K.S. Paek ◽  
...  
Keyword(s):  
Matrix Metalloproteinase ◽  
Nadph Oxidase ◽  
Human Gingival Fibroblasts ◽  
Matrix Metalloproteinase 2 ◽  
Peroxisome Proliferator ◽  
Gingival Fibroblasts ◽  
Peroxisome Proliferator Activated Receptor ◽  
Nadph Oxidase 4

scholarly journals Quercetin Inhibits Inflammatory Response Induced by LPS from Porphyromonas gingivalis in Human Gingival Fibroblasts via Suppressing NF-κB Signaling Pathway

2019 ◽  
Vol 2019 ◽  
pp. 1-10 ◽  
Author(s):  
Gang Xiong ◽  
Wansheng Ji ◽  
Fei Wang ◽  
Fengxiang Zhang ◽  
Peng Xue ◽  
...  
Keyword(s):  
Porphyromonas Gingivalis ◽  
Interleukin 1 ◽  
Human Gingival Fibroblasts ◽  
Enzyme Linked Immunosorbent Assay ◽  
Peroxisome Proliferator ◽  
Mrna Levels ◽  
Dependent Manner ◽  
Gingival Fibroblasts ◽  
Peroxisome Proliferator Activated Receptor ◽  
Anti Inflammatory

Quercetin, a natural flavonol existing in many food resources, has been reported to be an effective antimicrobial and anti-inflammatory agent for restricting the inflammation in periodontitis. In this study, we aimed to investigate the anti-inflammatory effects of quercetin on Porphyromonas gingivalis (P. gingivalis) lipopolysaccharide- (LPS-) stimulated human gingival fibroblasts (HGFs). HGFs were pretreated with quercetin prior to LPS stimulation. Cell viability was evaluated by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay. The levels of inflammatory cytokines, including interleukin-1β (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α), along with chemokine interleukin-8 (IL-8), were determined by enzyme-linked immunosorbent assay (ELISA). The mRNA levels of IL-1β, IL-6, IL-8, TNF-α, IκBα, p65 subunit of nuclear factor-kappa B (NF-κB), peroxisome proliferator-activated receptor-γ (PPAR-γ), liver X receptor α (LXRα), and Toll-like receptor 4 (TLR4) were measured by real-time quantitative PCR (RT-qPCR). The protein levels of IκBα, p-IκBα, p65, p-p65, PPAR-γ, LXRα, and TLR4 were characterized by Western blotting. Our results demonstrated that quercetin inhibited the LPS-induced production of IL-1β, IL-6, IL-8, and TNF-α in a dose-dependent manner. It also suppressed LPS-induced NF-κB activation mediated by TLR4. Moreover, the anti-inflammatory effects of quercetin were reversed by the PPAR-γ antagonist of GW9662. In conclusion, these results suggested that quercetin attenuated the production of IL-1β, IL-6, IL-8, and TNF-α in P. gingivalis LPS-treated HGFs by activating PPAR-γ which subsequently suppressed the activation of NF-κB.

Crosstalk Between Human Monocytic U937 Cells and Gingival Fibroblasts in Coculturally Enhanced Matrix Metalloproteinase-2 Expression

2016 ◽  
Vol 87 (10) ◽  
pp. 1228-1237 ◽  
Author(s):  
Po-Jan Kuo ◽  
Hsiao-Lun Lin ◽  
Chi-Yu Lin ◽  
Yu-Tang Chin ◽  
Hsiao-Pei Tu ◽  
...  
Keyword(s):  
Matrix Metalloproteinase ◽  
Matrix Metalloproteinase 2 ◽  
U937 Cells ◽  
Gingival Fibroblasts

Upregulation of matrix metalloproteinase-1 production by prostaglandin F2alpha in human gingival fibroblasts

2001 ◽  
Vol 36 (5) ◽  
pp. 334-339 ◽  
Author(s):  
Kazuyuki Noguchi ◽  
Yumiko Tominaga ◽  
Kenji Matsushita ◽  
Yuichi Izumi ◽  
Hirahito Endo ◽  
...  
Keyword(s):  
Matrix Metalloproteinase ◽  
Human Gingival Fibroblasts ◽  
Gingival Fibroblasts ◽  
Prostaglandin F2alpha ◽  
Matrix Metalloproteinase 1
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