DNA MICROARRAY APPLICATION IN ECOTOXICOLOGY: EXPERIMENTAL DESIGN, MICROARRAY SCANNING, AND FACTORS AFFECTING TRANSCRIPTIONAL PROFILES IN A SMALL FISH SPECIES

2008 ◽  
Vol 27 (3) ◽  
pp. 652 ◽  
Author(s):  
Rong-Lin Wang ◽  
Adam Biales ◽  
David Bencic ◽  
David Lattier ◽  
Mitch Kostich ◽  
...  
Keyword(s):  
Experimental Design ◽  
Dna Microarray ◽  
Fish Species ◽  
Small Fish ◽  
Factors Affecting ◽  
Transcriptional Profiles

DNA MICROARRAY APPLICATION IN ECOTOXICOLOGY: EXPERIMENTAL DESIGN, MICROARRAY SCANNING, AND FACTORS IMPACTING TRANSCRIPTIONAL PROFILES IN A SMALL FISH SPECIES

2007 ◽  
Vol preprint (2007) ◽  
pp. 1
Author(s):  
Rong-Lin Wang ◽  
Adam Biales ◽  
David Bencic ◽  
David Lattier ◽  
Mitch Kostich ◽  
...  
Keyword(s):  
Experimental Design ◽  
Dna Microarray ◽  
Fish Species ◽  
Small Fish ◽  
Transcriptional Profiles

Use of an experimental design to optimise the saponification reaction and the quantification of vitamins A1 and A2 in whole fish

2021 ◽  
Author(s):  
Sengly Sroy ◽  
Adrien Servent ◽  
Wichien Sriwichai ◽  
Sokneang In ◽  
Sylvie Avallone
Keyword(s):  
Experimental Design ◽  
Vitamin A ◽  
Fish Species ◽  
Retinyl Palmitate ◽  
Reliable Estimate ◽  
Small Fish ◽  
The Matrix ◽  
Nutritional Needs ◽  
Effects Of Temperature ◽  
Asean Countries

Abstract. In ASEAN countries, small freshwater fish species contribute to the nutritional needs of people with few livelihoods by providing them with significant amounts of protein, fat, vitamins and minerals. Some species are eaten whole (with their organs, skin, bones, head and eyes). To estimate the vitamin A content of these foods, conventional saponification has been applied but has not been able to fully release the retinol. Our objective was to optimise the conditions of vitamin A saponification in whole fish to have a reliable estimate of their contribution to intakes. The effects of temperature and saponification time on the retinol quantification of whole fish were evaluated using a two-factor experimental design. Reaction time had a significant effect on the saponification of standard retinyl palmitate and whole fish (p≤0.05). For whole fish, the best conditions for the saponification were to heat the samples to 80 °C for 43 minutes. Under these conditions, the retinol is well liberated from the matrix and protected from degradation and isomerisation reactions. The time-temperature couple used is more intense than that recommended for quantifying vitamin A in milk or enriched margarines. The protective effect of the food matrix against the release of retinol is evident. Vitamin A2 alcohol (3,4-didehydroretinol) was detected in five species and the overall vitamin A contents ranged from 9.6 to 737.5 μg RE/100 g in species frequently consumed in Cambodia. The two species of small fish consumed whole were the ones that contained significantly more vitamin A among the ten tested (p≤0.05). Highlights: Vitamin A2 alcohol was quantified in five fish species. The official saponification partially released retinol in whole fish. The optimised reaction required heating the sample to 80 °C for 43 min.

Size Changes, Mortality, and Equilibrium Yields in an Exploited Stock of American Plaice (Hippoglossoides platessoides)

1969 ◽  
Vol 26 (5) ◽  
pp. 1205-1235 ◽  
Author(s):  
P. M. Powles
Keyword(s):  
Mesh Size ◽  
Food Competition ◽  
Mortality Factor ◽  
Otter Trawl ◽  
Small Fish ◽  
Large Fish ◽  
Age Composition ◽  
Instantaneous Rate ◽  
Factors Affecting ◽  
Ecological Dominance

Records from research surveys and commercial landings for American plaice from the Magdalen Shallows, or the southwestern Gulf of St. Lawrence, showed decreases in percentage of old, large fish and in catch per unit of effort from 1955 to 1962. The changes in age composition were clearly relatable to the fishery, which was mainly by otter trawl. Ricker models indicated that wastage of deck-exposed subcommercial plaice is currently a greater mortality factor than predation by cod. Increased landings in particular years were associated with successful year-classes. Other factors affecting landings were annual differences in fleet dispersal, which were related to movements of cod, a cohabiting species. No clear decrease or increase in absolute recruitment of plaice was demonstratable because quantitative comparisons of research surveys by different vessels using different gears and with different skippers, would be misleading.Mortality estimates of adult plaice by three methods showed agreement, indicating that instantaneous rate of natural mortality was between 0.09 and 0.13. The instantaneous rate of fishing for 1957–62 was 0.46. Increasing mesh-size would reduce cod catches and have little effect in conserving plaice, but marketing small fish would increase plaice landings. Voluntary release of small plaice promptly on capture would help maintain the stock, as would reduction in numbers of large cod, the main predator of small plaice. Increased numbers of small cod since 1959, as reported by other workers, could result in greater food competition with small plaice, effectively reinforcing the ecological dominance of cod over plaice.

Higher is Faster

2015 ◽  
Vol 33 (2) ◽  
pp. 179-198 ◽  
Author(s):  
Hila Tamir-Ostrover ◽  
Zohar Eitan
Keyword(s):  
Experimental Design ◽  
Empirical Studies ◽  
Music Training ◽  
Factors Affecting ◽  
Pitch Change ◽  
Musical Pitch ◽  
Pitch Direction ◽  
Pitch Structure ◽  
Musical Tempo

While determining an appropriate tempo is crucial to music performers, composers and listeners, few empirical studies have investigated the musical factors affecting tempo choices. In two experiments we examined how aspects of musical pitch affect tempo choice, by asking participants (musically trained and untrained) to adjust the tempi of melodic sequences varying in pitch register and pitch direction, as well as sequences typically associated with specific registers in common period music. In Experiment 1, faster tempi were assigned to higher registers. Specific melodic direction (rise vs. fall) did not affect tempo preferences; nevertheless, pitch change in both directions elicited faster tempi than a repeating, unchanging pitch. The effect of register on tempo preference was stronger for participants with music training, and also (unexpectedly) for female participants. In Experiment 2, melodic figures typically related to lower and higher parts in common-period music were associated with slower and faster tempi, respectively. Results support a “holistic” notion of musical tempo, viewing the choice of proper tempo as determined by interactions among diverse musical dimensions, including aspects of pitch structure, rather than by rhythmic considerations alone. The experimental design presented here can be further applied to explore the effects of other musical parameters on tempo preferences.

Screening of factors affecting reactive blue 19 decolorization byGanodermasp.using fractional factorial experimental design

2010 ◽  
Vol 22 (1-3) ◽  
pp. 22-29 ◽  
Author(s):  
Mehran Mohammadian Fazli ◽  
Ali Reza Mesdaghinia ◽  
Kazem Naddafi ◽  
Simin Nasseri ◽  
Masoud Yunesian ◽  
...  
Keyword(s):  
Experimental Design ◽  
Fractional Factorial ◽  
Factorial Experimental Design ◽  
Factors Affecting ◽  
Reactive Blue 19 ◽  
Fractional Factorial Experimental Design

scholarly journals Use of food resources by small fish species in Neotropical rivers: responses to spatial and temporal variations

2014 ◽  
Vol 31 (5) ◽  
pp. 435-444 ◽  
Author(s):  
Jislaine Cristina da Silva ◽  
Éder André Gubiani ◽  
Rosilene Luciana Delariva
Keyword(s):  
Fish Species ◽  
Temporal Variations ◽  
Food Resources ◽  
Spatial And Temporal Variations ◽  
Small Fish

scholarly journals Nest-site selection in a fish species with paternal care

Hydrobiologia ◽  
2020 ◽  
Author(s):  
Theo C. M. Bakker ◽  
Beat Mundwiler
Keyword(s):  
Dissolved Oxygen ◽  
Water Temperature ◽  
Oxygen Concentration ◽  
Fish Species ◽  
Nest Site ◽  
Paternal Care ◽  
Small Fish ◽  
Dissolved Oxygen Concentration ◽  
Nest Sites ◽  
Higher Temperature

AbstractFish that perform paternal care may increase their fitness by choosing nest sites that enhance survival and development of embryos. We studied nest-site choice with respect to dissolved oxygen concentration and water temperature in males of the three-spined stickleback (Gasterosteus aculeatus), a small fish species with exclusive male parental care that usually breeds in the littoral zone of freshwaters of the Northern hemisphere. Fathers oxygenate the embryos by fanning movements of their pectoral fins. We expected choice for conditions at potential nest sites that would benefit offspring development, i.e., higher temperature and higher dissolved oxygen concentration. In the laboratory, we offered males a choice between two potential nest sites that differed in dissolved oxygen concentration or water temperature. Males preferred to build a nest at sites with a higher dissolved oxygen level or higher temperature and thus chose sites that would promote embryo development.

scholarly journals Distinct Transcriptional Profiles of Adrenocortical Tumors Uncovered by DNA Microarray Analysis

2003 ◽  
Vol 162 (2) ◽  
pp. 521-531 ◽  
Author(s):  
Thomas J. Giordano ◽  
Dafydd G. Thomas ◽  
Rork Kuick ◽  
Michelle Lizyness ◽  
David E. Misek ◽  
...  
Keyword(s):  
Microarray Analysis ◽  
Dna Microarray ◽  
Transcriptional Profiles ◽  
Adrenocortical Tumors ◽  
Dna Microarray Analysis
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