DNA MICROARRAY APPLICATION IN ECOTOXICOLOGY: EXPERIMENTAL DESIGN, MICROARRAY SCANNING, AND FACTORS IMPACTING TRANSCRIPTIONAL PROFILES IN A SMALL FISH SPECIES

2007 ◽  
Vol preprint (2007) ◽  
pp. 1
Author(s):  
Rong-Lin Wang ◽  
Adam Biales ◽  
David Bencic ◽  
David Lattier ◽  
Mitch Kostich ◽  
...  
Keyword(s):  
Experimental Design ◽  
Dna Microarray ◽  
Fish Species ◽  
Small Fish ◽  
Transcriptional Profiles

Use of an experimental design to optimise the saponification reaction and the quantification of vitamins A1 and A2 in whole fish

2021 ◽  
Author(s):  
Sengly Sroy ◽  
Adrien Servent ◽  
Wichien Sriwichai ◽  
Sokneang In ◽  
Sylvie Avallone
Keyword(s):  
Experimental Design ◽  
Vitamin A ◽  
Fish Species ◽  
Retinyl Palmitate ◽  
Reliable Estimate ◽  
Small Fish ◽  
The Matrix ◽  
Nutritional Needs ◽  
Effects Of Temperature ◽  
Asean Countries

Abstract. In ASEAN countries, small freshwater fish species contribute to the nutritional needs of people with few livelihoods by providing them with significant amounts of protein, fat, vitamins and minerals. Some species are eaten whole (with their organs, skin, bones, head and eyes). To estimate the vitamin A content of these foods, conventional saponification has been applied but has not been able to fully release the retinol. Our objective was to optimise the conditions of vitamin A saponification in whole fish to have a reliable estimate of their contribution to intakes. The effects of temperature and saponification time on the retinol quantification of whole fish were evaluated using a two-factor experimental design. Reaction time had a significant effect on the saponification of standard retinyl palmitate and whole fish (p≤0.05). For whole fish, the best conditions for the saponification were to heat the samples to 80 °C for 43 minutes. Under these conditions, the retinol is well liberated from the matrix and protected from degradation and isomerisation reactions. The time-temperature couple used is more intense than that recommended for quantifying vitamin A in milk or enriched margarines. The protective effect of the food matrix against the release of retinol is evident. Vitamin A2 alcohol (3,4-didehydroretinol) was detected in five species and the overall vitamin A contents ranged from 9.6 to 737.5 μg RE/100 g in species frequently consumed in Cambodia. The two species of small fish consumed whole were the ones that contained significantly more vitamin A among the ten tested (p≤0.05). Highlights: Vitamin A2 alcohol was quantified in five fish species. The official saponification partially released retinol in whole fish. The optimised reaction required heating the sample to 80 °C for 43 min.

scholarly journals Use of food resources by small fish species in Neotropical rivers: responses to spatial and temporal variations

2014 ◽  
Vol 31 (5) ◽  
pp. 435-444 ◽  
Author(s):  
Jislaine Cristina da Silva ◽  
Éder André Gubiani ◽  
Rosilene Luciana Delariva
Keyword(s):  
Fish Species ◽  
Temporal Variations ◽  
Food Resources ◽  
Spatial And Temporal Variations ◽  
Small Fish

scholarly journals Nest-site selection in a fish species with paternal care

Hydrobiologia ◽  
2020 ◽  
Author(s):  
Theo C. M. Bakker ◽  
Beat Mundwiler
Keyword(s):  
Dissolved Oxygen ◽  
Water Temperature ◽  
Oxygen Concentration ◽  
Fish Species ◽  
Nest Site ◽  
Paternal Care ◽  
Small Fish ◽  
Dissolved Oxygen Concentration ◽  
Nest Sites ◽  
Higher Temperature

AbstractFish that perform paternal care may increase their fitness by choosing nest sites that enhance survival and development of embryos. We studied nest-site choice with respect to dissolved oxygen concentration and water temperature in males of the three-spined stickleback (Gasterosteus aculeatus), a small fish species with exclusive male parental care that usually breeds in the littoral zone of freshwaters of the Northern hemisphere. Fathers oxygenate the embryos by fanning movements of their pectoral fins. We expected choice for conditions at potential nest sites that would benefit offspring development, i.e., higher temperature and higher dissolved oxygen concentration. In the laboratory, we offered males a choice between two potential nest sites that differed in dissolved oxygen concentration or water temperature. Males preferred to build a nest at sites with a higher dissolved oxygen level or higher temperature and thus chose sites that would promote embryo development.

scholarly journals Distinct Transcriptional Profiles of Adrenocortical Tumors Uncovered by DNA Microarray Analysis

2003 ◽  
Vol 162 (2) ◽  
pp. 521-531 ◽  
Author(s):  
Thomas J. Giordano ◽  
Dafydd G. Thomas ◽  
Rork Kuick ◽  
Michelle Lizyness ◽  
David E. Misek ◽  
...  
Keyword(s):  
Microarray Analysis ◽  
Dna Microarray ◽  
Transcriptional Profiles ◽  
Adrenocortical Tumors ◽  
Dna Microarray Analysis

DNA MICROARRAY-BASED ECOTOXICOLOGICAL BIOMARKER DISCOVERY IN A SMALL FISH MODEL SPECIES

2007 ◽  
Vol preprint (2007) ◽  
pp. 1
Author(s):  
Rong-Lin Wang ◽  
David Bencic ◽  
Adam Biales ◽  
David Lattier ◽  
Mitch Kostich ◽  
...  
Keyword(s):  
Dna Microarray ◽  
Biomarker Discovery ◽  
Small Fish ◽  
Model Species ◽  
Fish Model

Length-weight relationships of four small fish species caught in the Heishui River, China

2016 ◽  
Vol 32 (2) ◽  
pp. 397-398 ◽  
Author(s):  
C. Wang ◽  
Y. Q. Liang ◽  
D.-M. Huang ◽  
M. Li
Keyword(s):  
Fish Species ◽  
Small Fish
Sign in / Sign up

Export Citation Format

Share Document