USE OF THE OLIGOCHAETE, LUMBRICULUS VARIEGATUS, AS A PREY ORGANISM FOR TOXICANT EXPOSURE OF FISH THROUGH THE DIET

2006 ◽  
Vol 25 (10) ◽  
pp. 2760 ◽  
Author(s):  
David R. Mount ◽  
Terry L. Highland ◽  
Vincent R. Mattson ◽  
Timothy D. Dawson ◽  
Kevin G. Lott ◽  
...  
2000 ◽  
Author(s):  
Roger M. Nisbet ◽  
Russell J. Schmitt ◽  
Erick B. Muller

2017 ◽  
Vol 27 (3) ◽  
pp. 319-324 ◽  
Author(s):  
Eleanor L Leavens ◽  
Leslie M Driskill ◽  
Neil Molina ◽  
Thomas Eissenberg ◽  
Alan Shihadeh ◽  
...  

IntroductionOne possible reason for the rapid proliferation of waterpipe (WP) smoking is the pervasive use of flavoured WP tobacco. To begin to understand the impact of WP tobacco flavours, the current study examined the impact of a preferred WP tobacco flavour compared with a non-preferred tobacco flavoured control on user’s smoking behaviour, toxicant exposure and subjective smoking experience.MethodThirty-six current WP smokers completed two, 45-minute ad libitum smoking sessions (preferred flavour vs non-preferred tobacco flavour control) in a randomised cross-over design. Participants completed survey questionnaires assessing subjective smoking experience, exhaled carbon monoxide (eCO) testing, and provided blood samples for monitoring plasma nicotine. WP smoking topography was measured continuously throughout the smoking session.ResultsWhile participants reported an enhanced subjective smoking experience including greater interest in continued use, greater pleasure derived from smoking, increased liking and enjoyment, and willingness to continue use after smoking their preferred WP tobacco flavour (p values <0.05), no significant differences were observed in nicotine and carbon monoxide boost between flavour preparations. Greater average puff volume (p=0.018) was observed during the non-preferred flavour session. While not significant, measures of flow rate, interpuff interval (IPI), and total number of puffs were trending towards significance (p values <0.10), with decreased IPI and greater total number of puffs during the preferred flavour session.DiscussionThe current study is the first to examine flavours in WP smoking by measuring preferred versus control preparations to understand the impact on subjective experience, smoking behaviour and toxicant exposure. The pattern of results suggests that even this relatively minor manipulation resulted in significant changes in subjective experience. These results indicate a possible need for regulations restricting flavours in WP tobacco as with combustible cigarettes.


Gene Reports ◽  
2021 ◽  
Vol 23 ◽  
pp. 101050
Author(s):  
Aldo Arturo Tellez-Garcia ◽  
Roberto Álvarez-Martínez ◽  
Juana María López-Martínez ◽  
Fausto Arellano-Carbajal

2021 ◽  
Vol 231 ◽  
pp. 105723
Author(s):  
Kristina Klein ◽  
Theresa Piana ◽  
Tim Lauschke ◽  
Peter Schweyen ◽  
Georg Dierkes ◽  
...  

2008 ◽  
Vol 71 (3) ◽  
pp. 860-868 ◽  
Author(s):  
A.P.K. Jantunen ◽  
A. Tuikka ◽  
J. Akkanen ◽  
J.V.K. Kukkonen

2021 ◽  
Vol 33 (1) ◽  
Author(s):  
Marion Woermann ◽  
Julios Armand Kontchou ◽  
Bernd Sures

Abstract Background In order to protect aquatic environments and to reduce the presence of micropollutants in the global water cycle, wastewater treatment plants (WWTPs) often implement an additional treatment step. One of the most effective measures is the use of powdered activated carbon (PAC) as an adsorbent for micropollutants. This method provides sufficient elimination rates for several micropollutants and has been successfully employed in many WWTPs. Despite this success, there might be a drawback as the retention of the PAC in the WWTP can be challenging and losses of micropollutant-loaded PAC into the aquatic environment may occur. Upon emission, micropollutant-loaded PAC is expected to settle to the benthic zone of receiving waters, where sediment-dwelling organisms may ingest these particles. Therefore, the present study investigated possible adverse effects of micropollutant-loaded PAC from a WWTP as compared to unloaded (native) and diclofenac-loaded PAC on the sediment-dwelling annelid Lumbriculus variegatus. Results Native PAC induced the strongest effects on growth (measured as biomass) and reproduction of the annelids. The corresponding medium effective concentrations (EC50) were 1.7 g/kg and 1.8 g/kg, respectively. Diclofenac-loaded PAC showed lower effects with an EC50 of 2.5 g/kg for growth and EC50 of 3.0 g/kg for reproduction. Although tested at the same concentrations, the micropollutant-loaded PAC from the WWTP did not lead to obvious negative effects on the endpoints investigated for L.variegatus and only a slight trend of a reduced growth was detected. Conclusion We did not detect harmful effects on L. variegatus caused by the presence of MP-loaded PAC from a WWTP which gives an auspicious perspective for PAC as an advanced treatment option.


2007 ◽  
Vol 8 (1) ◽  
pp. 59-66 ◽  
Author(s):  
Olli-Pekka Penttinen ◽  
Johanna Kilpi-Koski ◽  
Maritta Jokela ◽  
Katri Toivainen ◽  
Ari Väisänen

2005 ◽  
Vol 71 (1) ◽  
pp. 51-57 ◽  
Author(s):  
Catalina Arango Pinedo ◽  
Barth F. Smets

ABSTRACT The effects of restriction proficiency and premating exposure to toxicants on conjugal transfer of the TOL plasmid between Pseudomonas spp. was investigated by examinations of filter matings. A Pseudomonas putida KT2442-derived strain carrying a gfp-tagged variant of the TOL plasmid was used as a donor, and both restriction-deficient (PAO1162N) and -proficient (PAO2002N) Pseudomonas aeruginosa strains were used as recipients. The in situ enumeration of conjugation events allowed us to obtain frequency estimates that were unbiased by transconjugant growth or plasmid retransfer. We observed a strong dependence of the plasmid transfer frequency on the initial donor-to-recipient ratio of surface matings, which invalidated the use of mass action-based plasmid transfer kinetic estimators. Careful control of the initial parental cell densities permitted evaluations of the true effects of restriction proficiency and toxicant exposure on TOL transfer. At standard donor-to-recipient ratios (10−3 for PAO1162N and 2 � 101 for PAO2002N) and total cell densities (105 cells/mm2 for PAO1162N and 106 cells/mm2 for PAO2002N), plasmid transfer frequencies without toxicant exposure were approximately 10−7 (events/mm2)−1 for PAO1162N and 10−11 (events/mm2)−1 for PAO2002N based on in situ observations of conjugation events. The enumeration of transconjugants via selective plating yielded transfer frequencies that were up to 1 order of magnitude lower. Premating exposure to sodium dodecyl sulfate (1 to 10 mM) significantly increased the transfer frequency for the restriction-proficient strain PAO2002N (P < 0.05) but not for the restriction-deficient strain PAO1162N. On the other hand, premating exposure to ethanol, toluene, or phenol had no positive effect on the plasmid transfer frequency. Clearly, restriction proficiency provides a strong barrier to interspecific transfer of the TOL plasmid, and this barrier was only marginally attenuated by recipient exposure to toxicants within the ranges examined.


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