QUANTITATIVE BIO-IMAGING ANALYSIS FOR EVALUATION OF SEXUAL DIFFERENTIATION IN GERM CELLS OF olvas-GFP/ST-II YI MEDAKA (ORYZIAS LATIPES) NANOINJECTED IN OVO WITH ETHINYLESTRADIOL

2005 ◽  
Vol 24 (1) ◽  
pp. 70 ◽  
Author(s):  
Takeshi Hano ◽  
Yuji Oshima ◽  
Toshiaki Oe ◽  
Masato Kinoshita ◽  
Minoru Tanaka ◽  
...  
Keyword(s):  
Germ Cells ◽  
Sexual Differentiation ◽  
Oryzias Latipes ◽  
Imaging Analysis ◽  
In Ovo ◽  
Bio Imaging

scholarly journals In ovo exposure to o,p -DDE affects sexual development but not sexual differentiation in Japanese medaka (Oryzias latipes).

2003 ◽  
Vol 111 (1) ◽  
pp. 29-32 ◽  
Author(s):  
Diana M Papoulias ◽  
Sergio A Villalobos ◽  
John Meadows ◽  
Douglas B Noltie ◽  
John P Giesy ◽  
...  
Keyword(s):  
Sexual Development ◽  
Sexual Differentiation ◽  
Oryzias Latipes ◽  
Japanese Medaka ◽  
In Ovo

Specific expression of Olpiwi1 and Olpiwi2 in medaka (Oryzias latipes) germ cells

2012 ◽  
Vol 418 (4) ◽  
pp. 592-597 ◽  
Author(s):  
Haobin Zhao ◽  
Jundan Duan ◽  
Nana Cheng ◽  
Yoshitaka Nagahama
Keyword(s):  
Germ Cells ◽  
Oryzias Latipes ◽  
Specific Expression

scholarly journals Müllerian Inhibiting Substance Is Required for Germ Cell Proliferation during Early Gonadal Differentiation in Medaka (Oryzias latipes)

Endocrinology ◽  
2007 ◽  
Vol 149 (4) ◽  
pp. 1813-1819 ◽  
Author(s):  
Eri Shiraishi ◽  
Norifumi Yoshinaga ◽  
Takeshi Miura ◽  
Hayato Yokoi ◽  
Yuko Wakamatsu ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Germ Cell ◽  
Germ Cells ◽  
Sex Differentiation ◽  
Somatic Cells ◽  
Oryzias Latipes ◽  
Cell Number ◽  
Gonadal Differentiation ◽  
Loss Of Function ◽  
Germ Cell Proliferation

Müllerian inhibiting substance (MIS) is a glycoprotein belonging to the TGF-β superfamily. In mammals, MIS is responsible for the regression of Müllerian ducts in the male fetus. However, the role of MIS in gonadal sex differentiation of teleost fish, which have no Müllerian ducts, has yet to be clarified. In the present study, we examined the expression pattern of mis and mis type 2 receptor (misr2) mRNAs and the function of MIS signaling in early gonadal differentiation in medaka (teleost, Oryzias latipes). In situ hybridization showed that both mis and misr2 mRNAs were expressed in the somatic cells surrounding the germ cells of both sexes during early sex differentiation. Loss-of-function of either MIS or MIS type II receptor (MISRII) in medaka resulted in suppression of germ cell proliferation during sex differentiation. These results were supported by cell proliferation assay using 5-bromo-2′-deoxyuridine labeling analysis. Treatment of tissue fragments containing germ cells with recombinant eel MIS significantly induced germ cell proliferation in both sexes compared with the untreated control. On the other hand, culture of tissue fragments from the MIS- or MISRII-defective embryos inhibited proliferation of germ cells in both sexes. Moreover, treatment with recombinant eel MIS in the MIS-defective embryos dose-dependently increased germ cell number in both sexes, whereas in the MISRII-defective embryos, it did not permit proliferation of germ cells. These results suggest that in medaka, MIS indirectly stimulates germ cell proliferation through MISRII, expressed in the somatic cells immediately after they reach the gonadal primordium.

Sexual differentiation of germ cell deficient gonads in the medaka,Oryzias latipes

2001 ◽  
Vol 290 (4) ◽  
pp. 402-410 ◽  
Author(s):  
Ai Shinomiya ◽  
Satoshi Hamaguchi ◽  
Naoki Shibata
Keyword(s):  
Germ Cell ◽  
Sexual Differentiation ◽  
Oryzias Latipes

Sex differentiation of germ cells in the teleost, Oryzias latipes, during normal embryonic development

Development ◽  
1972 ◽  
Vol 28 (2) ◽  
pp. 385-395
Author(s):  
Noriyuki Satoh ◽  
Nobuo Egami
Keyword(s):  
Germ Cells ◽  
Meiotic Prophase ◽  
Sex Differentiation ◽  
Primordial Germ Cells ◽  
Oryzias Latipes ◽  
The Other ◽  
Histological Structure ◽  
The Mean ◽  
Further Development ◽  
Normal Embryonic Development

Mitotic and meiotic activities of germ cells during early development in the medaka, Oryzias latipes, are dealt with in this report. Primordial germ cells were obviously distinguishable from somatic cells 3 days after fertilization and began to proliferate about 7 days after fertilization. The mean number of primordial germ cells increased during a period of 7–10 days after fertilization, reaching about 90 immediately before hatching. Newly hatched fry could be classified into two types according to the number and the nucleic activity of germ cells in the gonadal rudiment. One type consisted of fry containing about 100 germ cells and no cells in the meiotic prophase. In the other type of fry the number of germ cells increased by mitotic divisions and some of the cells began to enter into the meiotic prophase. During the course of further development the fry of the former type differentiated into males and the latter into females. Therefore it can be concluded that the morphological sex differentiation of germ cells occurs at the time of hatching. However, no sexual differences in the histological structure of somatic elements in the gonad are observable at that time.

scholarly journals Automated 3D bio-imaging analysis of nuclear organization by NucleusJ 2.0

Nucleus ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 315-329
Author(s):  
Tristan Dubos ◽  
Axel Poulet ◽  
Céline Gonthier-Gueret ◽  
Guillaume Mougeot ◽  
Emmanuel Vanrobays ◽  
...  
Keyword(s):  
Nuclear Organization ◽  
Imaging Analysis ◽  
Bio Imaging
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