Sexual differentiation of germ cell deficient gonads in the medaka,Oryzias latipes

2001 ◽  
Vol 290 (4) ◽  
pp. 402-410 ◽  
Author(s):  
Ai Shinomiya ◽  
Satoshi Hamaguchi ◽  
Naoki Shibata
Keyword(s):  
Germ Cell ◽  
Sexual Differentiation ◽  
Oryzias Latipes

scholarly journals Exposure to Diethylstilbestrol During Embryonic and Larval Stages of Medaka Fish (Oryzias latipes) Leads to Sex Reversal in Genetic Males and Reduced Gonad Weight in Genetic Females

Endocrinology ◽  
2011 ◽  
Vol 152 (2) ◽  
pp. 707-717 ◽  
Author(s):  
Bindhu Paul-Prasanth ◽  
Yasushi Shibata ◽  
Ryo Horiguchi ◽  
Yoshitaka Nagahama
Keyword(s):  
Cell Proliferation ◽  
Germ Cell ◽  
Sexual Differentiation ◽  
Ovarian Development ◽  
Oryzias Latipes ◽  
Sex Reversal ◽  
Short Exposure ◽  
Specific Gene ◽  
Gonad Weight ◽  
Germ Cell Proliferation

Abstract Molecular and cellular mechanisms involved in artificially induced ovarian differentiation were analyzed by exposing embryos of medaka (Oryzias latipes) to a potent nonsteroidal estrogen, diethylstilbestrol (DES). Embryos were exposed for short-exposure (SE) [from 0 to 8 d postfertilization (dpf)] and long-exposure (LE) periods (from 0 to 18/28 dpf) to 1 ng/ml of DES, and status of sexual differentiation in somatic and germ cells of these gonads was analyzed at 8, 18, and 28 dpf by histology, cell proliferation assays, TUNEL assay, and in situ hybridization using sex-specific somatic and germ cell markers. Additionally, gonads of exposed fry were examined after withdrawal of DES to see whether effects of DES in exposed fish were reversible or not. DES induced germ cell proliferation and meiosis in XY fry of SE and LE groups. However, SE induced only a partial reduction in expression of gonadal soma-derived factor, the male-dominant somatic cell marker, and was not sufficient to induce ovarian development after withdrawal of DES. On the contrary, LE resulted in complete loss of such male-specific gene expression in somatic cells of XY gonads, and these gonads underwent sustained ovarian development even after withdrawal of DES. Importantly, LE to DES affected germ cell proliferation in XX gonads adversely during early stages of sexual differentiation, leading to reduced gonad weight in adulthood. Interestingly, apoptosis was not the cause for reduction in germ cell number. Taken together, these results indicated that DES exposure has long-lasting effects on the gonadal development in genetic males (sex reversal) and females (reduced gonad weight) of medaka.

scholarly journals Müllerian Inhibiting Substance Is Required for Germ Cell Proliferation during Early Gonadal Differentiation in Medaka (Oryzias latipes)

Endocrinology ◽  
2007 ◽  
Vol 149 (4) ◽  
pp. 1813-1819 ◽  
Author(s):  
Eri Shiraishi ◽  
Norifumi Yoshinaga ◽  
Takeshi Miura ◽  
Hayato Yokoi ◽  
Yuko Wakamatsu ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Germ Cell ◽  
Germ Cells ◽  
Sex Differentiation ◽  
Somatic Cells ◽  
Oryzias Latipes ◽  
Cell Number ◽  
Gonadal Differentiation ◽  
Loss Of Function ◽  
Germ Cell Proliferation

Müllerian inhibiting substance (MIS) is a glycoprotein belonging to the TGF-β superfamily. In mammals, MIS is responsible for the regression of Müllerian ducts in the male fetus. However, the role of MIS in gonadal sex differentiation of teleost fish, which have no Müllerian ducts, has yet to be clarified. In the present study, we examined the expression pattern of mis and mis type 2 receptor (misr2) mRNAs and the function of MIS signaling in early gonadal differentiation in medaka (teleost, Oryzias latipes). In situ hybridization showed that both mis and misr2 mRNAs were expressed in the somatic cells surrounding the germ cells of both sexes during early sex differentiation. Loss-of-function of either MIS or MIS type II receptor (MISRII) in medaka resulted in suppression of germ cell proliferation during sex differentiation. These results were supported by cell proliferation assay using 5-bromo-2′-deoxyuridine labeling analysis. Treatment of tissue fragments containing germ cells with recombinant eel MIS significantly induced germ cell proliferation in both sexes compared with the untreated control. On the other hand, culture of tissue fragments from the MIS- or MISRII-defective embryos inhibited proliferation of germ cells in both sexes. Moreover, treatment with recombinant eel MIS in the MIS-defective embryos dose-dependently increased germ cell number in both sexes, whereas in the MISRII-defective embryos, it did not permit proliferation of germ cells. These results suggest that in medaka, MIS indirectly stimulates germ cell proliferation through MISRII, expressed in the somatic cells immediately after they reach the gonadal primordium.

Germ cell-less expression in medaka (Oryzias latipes)

2003 ◽  
Vol 67 (1) ◽  
pp. 15-18 ◽  
Author(s):  
Stefan Scholz ◽  
H. Domaschke ◽  
A. Kanamori ◽  
K. Ostermann ◽  
G. Rödel ◽  
...  
Keyword(s):  
Germ Cell ◽  
Oryzias Latipes

β-estradiol 17-valerate affects embryonic development and sexual differentiation in Japanese medaka (Oryzias latipes)

2013 ◽  
Vol 134-135 ◽  
pp. 128-134 ◽  
Author(s):  
Bingli Lei ◽  
Jia Kang ◽  
Yingxin Yu ◽  
Jinmiao Zha ◽  
Wei Li ◽  
...  
Keyword(s):  
Embryonic Development ◽  
Sexual Differentiation ◽  
Oryzias Latipes ◽  
Japanese Medaka

scholarly journals GERM CELL NEOPLASIA IN PATIENTS WITH ABNORMAL SEXUAL DIFFERENTIATION

1986 ◽  
Vol 20 (11) ◽  
pp. 1183-1183
Author(s):  
M O Savage ◽  
D G Lowe ◽  
P G Ransley ◽  
I A Hughes ◽  
P G F Swift ◽  
...  
Keyword(s):  
Germ Cell ◽  
Sexual Differentiation
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