Seed Osmo-priming in Chickpea Enhances Seed Quality and Crop Performance under Normal and Water Deficit Conditions

Author(s):  
T.N. Tiwari ◽  
P.K. Katiyar

Background: The average productivity of chickpea is quite low (939 kg/ha) because of several factors including its cultivation in rain fed/low moisture and marginal lands, low seed replacement rate (25.4%), use of old and low-quality seed by the majority of farmers. This in turn gives poor germination, delayed emergence and sick seedlings that lead to poor yield. Osmo priming of seed with different in-organic salts has been reported to improve the germination, speed of emergence, seedling vigour, growth and yield of different vegetable and field crops but information’s on response of seed priming with in-organic salts on enhancement of seed quality parameters and crop performance of naturally aged seeds of chickpea is lacking over a wide range of environmental conditions including normal and water deficit conditions.Methods: An experiment was conducted under laboratory as well as in plastic pots with 05 seed osmo- priming level, two varieties of chickpea (Ujjawal and JG-14) and two moisture level (Normal at field capacity and water deficit at half of the field capacity) during rabi season 2017-18 and 2018-19.at the Research farm of ICAR- IIPR Kanpur (U.P.) to study the influence of seed osmo-priming with in- organic salts on seed quality parameters and crop performance under normal and water deficit conditions. The observations were recorded on seed quality, growth and crop efficiency parameters at their appropriate time.Result: Osmo-priming of one-year-old chickpea seeds with KNO3, MgSO4, Ca(NO3)2 at 0.2% solution and tap water for 06 hours significantly enhanced the seed quality parameters in both the varieties evaluated under normal as well as under water deficit conditions over their respective control. Amongst, the priming agents used, KNO3 performed better than MgSO4, Ca(NO3)2 and tap water in respect of most of the seed quality parameters studied. Variety Ujjawal responded better the priming treatments than JG-14 under both normal and water deficit conditions. Osmo-priming treatments also showed the positive response in the enhancement of Nitrogen balance index (NBI), chlorophyll, Flavonols, Stomatal conductance, photosynthetic rate and transpiration rate in both the varieties evaluated under normal as well as water deficit condition.

2021 ◽  
Vol 13 (2) ◽  
pp. 735-743
Author(s):  
Umesh Hiremath ◽  
Basave Gowda ◽  
Lokesh G.Y . ◽  
B. S. Ganiger

The continuous rise in global population demands more food production under limited land resources accompanied by climate change. More oversupply of quality seeds to meet ever-growing food demand is a highly challenging task. Seeds with low vigour level limit the growth and yield of crops at varied field conditions. Hence, an experiment was conducted to develop a technique for the fast and homogeneous growth of kabuli chickpea seeds. The priming treatments evaluated in the present study enhanced the various seed quality parameters of kabuli chickpea significantly when compared to control. Hydro-priming for 4 hours (at 20°C) and air drying for 72 hours showed better results compared to rest of the treatments both in fresh seeds which recorded higher first count (66.38%), germination (92.50%) root length (11.85 cm), shoot length (6.16), mean seedling length (18.01), dry seedling weight (747 mg), seedling vigour index-I (1665), higher speed of emergence (0.3992) and old seeds with higher first count (61.88%), germination (74.13%) root length (8.15 cm), shoot length (5.85 cm), mean seedling length (14.00 cm), seedling dry weight (611 mg), seedling vigour index-I (1056), higher speed of emergence (0.3814). The aged seeds have recorded 29 % increase in germination percentage in contrast to 8 percent increases in fresh seeds. Kabuli chickpea seeds could be subjected to hydropriming, an affordable, alternative, and eco-friendly technique for improving seed and seedling vigour of kabuli chickpea.


Author(s):  
Bela Joshi ◽  
P. K. Rai ◽  
Vandana Pandey

Seed germination, following seedling growth are basic but crucial steps in a plant life cycle which includes proper seed germination which is a basic pre-requisite for a better crop growth and yield. The experiment was conducted in Vegetable Science Department under the Defence Institute of Bio-Energy and Research (DIBER), (DRDO), Pithoragarh, Uttarakhand in the year 2020-2021 to standardize the best treatment of seed priming specific to Spinach (Spinacia oleracea). An investigation was conducted both in a laboratory and on the field with the same treatment that was: (T1) Distilled water, (T2) 1%NaCl, (T3) 1%H2O2, (T4) 1%Na2CO3, (T5) Tap water, (T6) 1%DAP, (T7) Control. Seeds were soaked for 16 hrs and then dried to original moisture content for 2 days. It was found that all the priming treatments showed a significant difference with the control and the highest germination %(lab) with 71% was observed in seeds treated with DAP (T6) and the highest crop yield (field) were observed for 1% Na2CO3. Na2CO3 showed the best result in the field as well as in lab conditions based on different quality parameters followed by DAP. This study showed that seed priming treatments help in the enhancement of seed quality parameters, proved cost-effective and the most economical method. The experiment helps to improve the seed quality using different priming treatments which are cost-effective, economic, environment friendly and one of the quickest methods used for seed enhancement.


Author(s):  
T. N. Tiwari ◽  
D. K. Agarwal

Field experiments were conducted with four levels of seed priming including control and two varieties of pigeon pea at ICAR-Indian Institute of Seed Science, Mau during three consecutive years (2011-12 to 2013-14). One-year-old seeds of pigeon pea varieties (Bahar and Malviya-13) were primed with growth regulator (100 ppm GA3), in-organic salt (0.2% KNO3) and tap water (sanitized) separately for 06 hrs. and sown in field under RBD with 03 replications. Observations were recorded on seed quality parameters, biochemical attributes including nitrate assimilatory enzymes and activities of anti-oxidant enzymes during seedling stage. Seed quality parameters including germination, seedling growth and vigor indices were significantly enhanced through seed priming with GA3 followed by KNO3 and tap water over unprimed control. Biochemical attributes viz; chlorophyll a andb contents, were more influenced with GA3 priming followed by KNO3 and tap water whereas the proline accumulation was reduced with priming treatments and maximum reduction was noted with GA3 followed by KNO3 and tap water. Enhancement in nitrate assimilatory enzymes including nitrate and nitrite reductase activities was more with KNO3 priming followed by GA3 and tap water. Anti-oxidant enzymes activities including Catalase, Peroxidase and Super Oxide Dismutase were also increased significantly by KNO3 priming followed by GA3 and tap water over unprimed control.


2010 ◽  
Vol 100 (11) ◽  
pp. 1213-1221 ◽  
Author(s):  
Fatemeh Mastouri ◽  
Thomas Björkman ◽  
Gary E. Harman

Trichoderma spp. are endophytic plant symbionts that are widely used as seed treatments to control diseases and to enhance plant growth and yield. Although some recent work has been published on their abilities to alleviate abiotic stresses, specific knowledge of mechanisms, abilities to control multiple plant stress factors, their effects on seed and seedlings is lacking. We examined the effects of seed treatment with T. harzianum strain T22 on germination of seed exposed to biotic stress (seed and seedling disease caused by Pythium ultimum) and abiotic stresses (osmotic, salinity, chilling, or heat stress). We also evaluated the ability of the beneficial fungus to overcome physiological stress (poor seed quality induced by seed aging). If seed were not under any of the stresses noted above, T22 generally had little effect upon seedling performance. However, under stress, treated seed germinated consistently faster and more uniformly than untreated seeds whether the stress was osmotic, salt, or suboptimal temperatures. The consistent response to varying stresses suggests a common mechanism through which the plant–fungus association enhances tolerance to a wide range of abiotic stresses as well as biotic stress. A common factor that negatively affects plants under these stress conditions is accumulation of toxic reactive oxygen species (ROS), and we tested the hypothesis that T22 reduced damages resulting from accumulation of ROS in stressed plants. Treatment of seeds reduced accumulation of lipid peroxides in seedlings under osmotic stress or in aged seeds. In addition, we showed that the effect of exogenous application of an antioxidant, glutathione, or application of T22, resulted in a similar positive effect on seed germination under osmotic stress or in aged seed. This evidence supports the model that T. harzianum strain T22 increases seedling vigor and ameliorates stress by inducing physiological protection in plants against oxidative damage.


2019 ◽  
Vol 17 (1-2) ◽  
pp. 89-101
Author(s):  
MS Rana ◽  
MA Hossain ◽  
TA Urmi ◽  
S Ahmed ◽  
MM Haque ◽  
...  

Waterlogging is one the major abiotic stresses that affects growth and yield of blackgram. A field experiment was carried out at the experimental field of Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur, 1706 during kharif II season to evaluate 26 blackgram genotypes for understanding the flood tolerance in respect of yield and yield contributing characters. The experiment was laid out with a randomized complete block design with three replications. Water logging depth was 3-5 cm, created by irrigation from tap water and imposed at 29 days after emergence of seedlings which was maintained for 8 days. Multivariate analysis was performed involving eight qualitative plant characters. A wide range of variation was found for all the qualitative plant characters like plant height, plant dry weight, branches per plant, pods per plant, pod length, seeds per pod, 1000-seed weight and yield per plant. Flooding reduced all the growth parameters of blackgram but degree of reduction varied greatly over the blackgram genotypes. Considering yield performance, the genotypes BU Acc 25 showed the highest yield per plant (9.03 g) followed by the BU Acc 17 (6.47 g) and BU Acc 24 (6.17) under flooding . The yield reduction percent of the same genotypes were minimum (43.86, 60.82 and 65.96, respectively) compared to control which is very important for selection of those genotypes as a flooding tolerant. The Agriculturists 2019; 17(1-2) 89-101


Author(s):  
G. Sathiya Narayanan ◽  
M. Prakash ◽  
V. Rajesh Kumar

Pulses are one of the most important legume crops in India contributing significantly high quality protein. Among the legumes, black gram (Vigna mungo L. Hepper), is an important legume crop in our country belonging to the family leguminoceae. The low productivity in pulses may be due to the fact that they are normally grown in marginal lands with inadequate soil moisture and poor fertility status. To increase yield under these circumstances, integrated seed treatment is given as a presowing treatment. Hence, a study was attempted to find out the influence of integrated seed treatments on growth and yield parameters in black gram. The seeds of black gram cv VBN 4 were subjected to various seed treatments and treated seeds along with the control were evaluated for their seed quality, growth, gas exchange and yield parameters both in laboratory and field trials. The study revealed that seeds fortified with MgSO4 + polykote + carbendazim + dimethoate + bioinoculant (Pseudomononas fluorescens) + Rhizobium + Azophos + pelleting with DAP recorded higher seed yield and other parameters when compared to control.


Author(s):  
G. Sathiya Narayanan ◽  
M. Prakash ◽  
M. Reka

The present investigations were carried out to study the influence of various seed hardening treatments on growth and yield parameters in black gram under drought condition. The black gram cv.VBN 3 was imposed with various seed hardening treatments (i.e.,) MnSO4 @ 100 ppm, ZnSO4 @ 100 ppm, Cobalt nitrate @ 1 %, Sodium molybdate @ 100 ppm, KCl @ 100 ppm, Pungam leaf extract @ 1 %, Prosopis leaf extract @ 1% and GA3 @ 40 ppm. The above treated seeds along with control were evaluated for their seed quality parameters, growth, gas exchange and yield parameters under laboratory and drought field condition. The study revealed that seeds hardened with prosopis leaf extract @1% recorded higher seed yield, yield attributing characters and many seed quality characters as compared to other treatments and control under drought condition.


1995 ◽  
Vol 75 (3) ◽  
pp. 605-611 ◽  
Author(s):  
K. M. Volkmar ◽  
C. Chang

Hydrophilic super-absorbent polymers retain large amounts of plant-available moisture and have been promoted for use as soil amendments in drought-prone regions. This controlled-environment study evaluated the capacity of two commercial polymer gels, Grogel and Transorb, to mitigate the effects of recurring moderate water-deficit stress (dry-down to 50% field capacity before rewatering) on growth and yield of barley and canola. Rates of 0.03, 0.12, 0.47 and 1.87 g polymer kg−1 sandy loam soil (1, 4, 16 and 64 times the recommended commercial application rate) were tested. Plants were grown at a soil moisture content of approximately 50% of field capacity. Neither polymer was effective at the commercially recommended rate. Barley and canola grain yields were unaffected at any Grogel rate, and Transorb had no effect on barley grain yield. Grogel at the highest rate enhanced early shoot mass, mature biomass production and grain yield of barley and increased leaf RWC. Canola had greater early and late vegetative biomass, but pod yield was not increased by Grogel at any rate. Transorb was most effective at four times the recommended rate, significantly increasing tiller and fertile spike number and mature biomass production at that rate. Leaf RWC were unaffected by Transorb treatment. Grogel stimulated root growth of barley but had no effect on roots of canola. Both polymers tended to increase consumptive water use. Spatial restriction was found to drastically reduce the water retention of both polymers and limit the absorbency of both polymers in this study. The high rates of polymer required to elicit a crop yield response under relatively mild water-deficit conditions limit the value of these polymers for agricultural field use of the crop species tested. Key words: Barley, canola, drought, hydrophilic polymer, soil conditioner, water stress


1990 ◽  
Vol 115 (5) ◽  
pp. 722-727 ◽  
Author(s):  
J.J. Steiner ◽  
R.B. Hutmacher ◽  
A.B. Mantel ◽  
J.E. Ayars ◽  
S.S. Vail

Seed yield and quality of carrot (Daucus carota var. sativa DC.) were influenced by a wide range of water application regimes and levels. Irrigation treatments were imposed beginning at the time of extension of the primary umbel and extending throughout the reproductive development period. The three application regimes used were: 1) a high-frequency, low water deficit treatment [100% of daily accumulated crop evapotranspiration (ETc)]; 2) a series of five low-frequency (irrigated after 30 mm of accumulated ET,) application treatments with a range of water deficits from moderate to minimal (40% to 120% of ETc applied); and 3) a series of three treatments that had rapidly developing water deficits applied by terminating irrigation at 7, 5, and 2 weeks before harvest after being grown under low-stress conditions. Pure live seed (PLS) yield was optimized by different treatments within each of the three application regimes. Maximum yields were achieved with short-term (2-week) rapidly developing water deficits near harvest, moderate deficit irrigation with 60% to 80% of ETc applied after 30 mm of ETc, or with a low water deficit, high-frequency application. Seed germination percentage decreased as the amount of applied water increased. This effect was more pronounced in the later-developing umbel orders. However, seed quality measured as seedling root length was improved with increasing water application.


2016 ◽  
Vol 8 (1) ◽  
pp. 340-345
Author(s):  
N. Amruta ◽  
P. J. Devaraju ◽  
Mangalagowri Mangalagowri ◽  
S. P. Kiran ◽  
H. P. Ranjitha ◽  
...  

A field experiment was conducted to assess the response of nutrient levels and spacing on seed quality attributes of black gram cv. LBG-625 (Rashmi). Experimental results revealed that fertilizer application of 50:100:100 + Black gram rhizobia (250 g ha-1) + PSB- Bacillus megaterium (250 g ha-1) recorded highest test weight (39.27 g), germination (90.60%), root length (15.77 cm), shoot length (13.43 cm), mean seedling length (29.20 cm), mean seedling dry weight (57.99 mg), seedling vigour index-I (2656), seedling vigour index-II (525), total dehydrogenase activity (0.998), protein content (23.16%), field emergence (86.56 %) lowest electrical conductivity (0.813 dSm-1) were superior over other fertilizer treatments. Among the planting geometry 60 x 10 cm recorded more germination per cent (90.48 %) compared to 45 x 10 cm (87.64 %) and 30 x 10 cm (86.91 %). Interaction of nutrient levels and spacing through the application of 50:100:100 + black gram rhizobia (250 g ha-1) + PSB- B. megaterium (250 g ha-1) with planting geometry 60 x 10 cm recorded more mean seedling length (34.40 cm), mean seedling dry weight (58.30 mg),and field emergence (90.24 %) lowest electrical conductivity (0.776 dSm-1) compared to control. The application of 50:100:100 + Black gram rhizobia (250 g ha-1) + PSB- B. megaterium (250 g ha-1) with planting geometry 60 x 10 cm were considered as seed quality improvement approach in blackgram, therefore conjunctive use of inorganic fertilizers and biofertilizer may be suggested for higher seed quality parameters along with overall betterment of crop.


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