Seed Priming Influences the Seed Quality and Activities of Nitrate Assimilation and Anti-Oxidant Enzymes in Pigeon Pea Seedlings

Author(s):  
T. N. Tiwari ◽  
D. K. Agarwal

Field experiments were conducted with four levels of seed priming including control and two varieties of pigeon pea at ICAR-Indian Institute of Seed Science, Mau during three consecutive years (2011-12 to 2013-14). One-year-old seeds of pigeon pea varieties (Bahar and Malviya-13) were primed with growth regulator (100 ppm GA3), in-organic salt (0.2% KNO3) and tap water (sanitized) separately for 06 hrs. and sown in field under RBD with 03 replications. Observations were recorded on seed quality parameters, biochemical attributes including nitrate assimilatory enzymes and activities of anti-oxidant enzymes during seedling stage. Seed quality parameters including germination, seedling growth and vigor indices were significantly enhanced through seed priming with GA3 followed by KNO3 and tap water over unprimed control. Biochemical attributes viz; chlorophyll a andb contents, were more influenced with GA3 priming followed by KNO3 and tap water whereas the proline accumulation was reduced with priming treatments and maximum reduction was noted with GA3 followed by KNO3 and tap water. Enhancement in nitrate assimilatory enzymes including nitrate and nitrite reductase activities was more with KNO3 priming followed by GA3 and tap water. Anti-oxidant enzymes activities including Catalase, Peroxidase and Super Oxide Dismutase were also increased significantly by KNO3 priming followed by GA3 and tap water over unprimed control.

Author(s):  
Bela Joshi ◽  
P. K. Rai ◽  
Vandana Pandey

Seed germination, following seedling growth are basic but crucial steps in a plant life cycle which includes proper seed germination which is a basic pre-requisite for a better crop growth and yield. The experiment was conducted in Vegetable Science Department under the Defence Institute of Bio-Energy and Research (DIBER), (DRDO), Pithoragarh, Uttarakhand in the year 2020-2021 to standardize the best treatment of seed priming specific to Spinach (Spinacia oleracea). An investigation was conducted both in a laboratory and on the field with the same treatment that was: (T1) Distilled water, (T2) 1%NaCl, (T3) 1%H2O2, (T4) 1%Na2CO3, (T5) Tap water, (T6) 1%DAP, (T7) Control. Seeds were soaked for 16 hrs and then dried to original moisture content for 2 days. It was found that all the priming treatments showed a significant difference with the control and the highest germination %(lab) with 71% was observed in seeds treated with DAP (T6) and the highest crop yield (field) were observed for 1% Na2CO3. Na2CO3 showed the best result in the field as well as in lab conditions based on different quality parameters followed by DAP. This study showed that seed priming treatments help in the enhancement of seed quality parameters, proved cost-effective and the most economical method. The experiment helps to improve the seed quality using different priming treatments which are cost-effective, economic, environment friendly and one of the quickest methods used for seed enhancement.


Author(s):  
S. Kavitha ◽  
P. Srimathi

Background: Black gram is the important pulses crop, grown throughout the country; gives low seed yield mainly due to poor management and low soil fertility. Development of suitable technology is essential to enhance productivity. Seed priming is one such effective technology which enhance rapid and uniform emergence to achieve high vigour, leading to better stand establishment and yield. The current research aimed to study micronutrient seed priming and rhizobium coating on seed germination, seedling vigour, crop growth and seed yield in black gram.Methods: Blackgram seeds were primed with 10 different micronutrient solutions with different concentrations and evaluated for its quality parameters to find out suitable seed priming technique. Seeds from two best priming treatments were coated with rhizobium @ 30g/kg of seed and evaluated for its production potential and resultant seed quality under field condition.Result: Seed priming with 0.5% ZnSO4 at 1/3rd seed to solution ratio for 3 hours recorded higher germination (96%) and vigour index (3811) than control which recorded 84% seed germination and 2528 of vigour index under laboratory experiment. Field experiments revealed that, seeds primed with 0.5% ZnSO4 and coated with rhizobium @ 30g per kg of seeds improved seed yield by 13.2% over control. 


Author(s):  
Prashant Kumar Rai Kotamarthi Bhavana ◽  
G. M. Lal

The current experiment was conducted to evaluate the Performance of Organic amendments and botanical treatments on seed quality parameters of French bean under stimulated environmental conditions. An experiment was conducted in 2021 at postgraduate seed testing laboratory, Department of Genetics and Plant Breeding Naini Agriculture Institute, Sam Higginbottom University of Agriculture Technology and Sciences, Prayagraj. The French bean Cultivar Arka Bold seeds were used for priming. Organic treatments are designated as Panchagavya 2%, 4%,6%,8%, Agniasthra 2%,4%,6%,8% and Botanicals treatments are Tulasi leaf extract 5%, Aloevera leaf extract 5%, Vinca rosea leaf extract 5% and also did hydroprming treatment using distilled water respectively. Seeds were soaked in organic, botanicals and in distilled water for duration of 6 hours followed by shade drying. The results were obtained and it was found that all the treatments showed significant effect on quality parameters. The highest was found in panchgavya of all doses followed by tulasi leaf extract found to be best in Germination percentage, Speed of Germination, Root length, Shoot length, Seedling length, Seed vigour index-I, Seed vigour Index-II compared to control or untreated seeds which indicates the effect of organics and botanicals on physiological processes of the seeds. This study revealed that priming with organic amendments and botanicals can be effective in enhancing the seed quality parameters of french bean and they are low cost, effective and nature friendly and toxic free.


Author(s):  
K. Yashwanth Chandra ◽  
Arun Kumar Chaurasia

The present research study was conducted at the Seed testing laboratory of Sam Higginbottom Institute of Agriculture Technology and Sciences, Prayagraj, Uttar Pradesh in 2020-2021. The research study revealedthat effect of seed priming treatments with Panchagavya, Jeevamrutha, Beejamrutha and leaf extracts, Sodium molybdate on seed quality parameters of field pea was analyzed through Completely Randomized Design (CRD). Organic treatments are designated as Panchagavya 3% and 5%, Jeevamrutha 3% and 5%, Beejamrutha 5% are used as treatment 8Hrs respectively, botanical treatments are Tulasi leaf extract 6%, Lantana camara leaf extract for 6% are used as treatment for duration of 4 hours respectivelyin which the treatment T8- Sodium molybdate (3%) for 3 Hrs exhibited higher mean value for seed germination (76.75%), root length (6.49 cm), shoot length (7.84 cm), seedling length (14.33 cm), seed vigour index -I (1099.68) and Followed by value was exhibited by T6-Tulasi Leaf Extract (6%) with respect of Root length (6.79 cm)Seedling dry weight (1.725 g), Seed Vigour Index II (129.78), T0 (control) with respect of Germination percentage (71.75%) root length (6.30 cm), shoot length (6.60 cm) and seedling length (12.90 cm), seed vigour index I &II (925.60 & 98.13).


Author(s):  
K. Malarkodi ◽  
T. Vedhapriya ◽  
R. Umarani ◽  
M. Bhaskaran

Background: Seed priming technique involves soaking of seeds in priming agent to a point where germination related metabolic activities occur in the seeds followed by drying of imbibed seeds to original moisture to prevent the radical protrusion and facilitate storage of primed seed. Draining of priming agent and drying of large quantity of primed seeds is a laborious and time consuming process. The current study was aimed to standardize automation of seed priming process by utilizing house-hold top loading washing machine. Methods: The top loading washing machine referred to as Seed Priming Cabinet, contained a priming drum with provision for draining water. On completion of the soaking period, the ‘SPIN’ button was pressed by setting the duration of spin drying (1, 2, 3, 4 and 5 min.). Then the seeds were removed from the drum of priming cabinet and subjected to shade drying so as to reach the original moisture content and evaluated for seed quality parameters. Result: The blackgram seed subjected to conventional hydropriming was compared with automatic seed priming for 1, 2, 3, 4 and 5 min. and observed that hydropriming and spin drying for 3 min. was found to be optimum and the per cent increase in germination recorded over control was 18.0 per cent thus suggesting that automation of seed priming technology can be recommended for enhanced seed vigour and crop productivity of blackgram.


1998 ◽  
Vol 33 (2) ◽  
pp. 196-211 ◽  
Author(s):  
Livy Williams ◽  
D. J. Schotzko ◽  
L. E. O'Keeffe

Field experiments were conducted to determine the effects of herbivory, seed priming, and tillage practices on the growth response of Pisum sativum L. A factorial treatment design incorporated two levels of tillage treatment (moldboard and chisel plow), three levels of seed priming [Captan, PEG (polyethylene glycol) 8000, and Captan+PEG 8000], and four levels of herbivory by Sitona lineatus (L.) (caged controls, uncaged controls, 1 weevil/plant, and 8 weevils/plant). Pisum sativum stands at approximately 75% emergence were infested for 1 wk. Sitona lineatus infestations resulted in defoliation of approximately 5, 25, and 55% for the control, low, and high weevil densities, respectively. Conservation tillage led to increased surface residue but did not affect soil moisture, temperature, or compensatory growth response of P. sativum. Seed priming treatments, incorporating PEG, led to an undercompensatory growth response of P. sativum, as did high levels of S. lineatus defoliation. Improved seed priming methods that promote vigorous seedling growth may help minimize the impacts of S. lineatus and suboptimal soil conditions on P. sativum.


2021 ◽  
Vol 911 (1) ◽  
pp. 012086
Author(s):  
Ramlah Arief ◽  
Fauziah Koes ◽  
Oom Komalasari ◽  
Fatmawati

Abstract Seed priming or invigoration is presowing treatments of improving germination aimed to reduce the time from sowing to emergence and improving emergence uniformity. Research on seed priming was conducted Indonesian Cereal Research Institute (ICERI) seed laboratory from January until March 2021 to evaluate the effect of seed priming on sorghum seed vigor. Seed quality parameters includes germination percentages, germination rate, shoot and primary root length, seedling dryweight, Sorghum seed variety of Suri 4 were used as seed material. Results showed that among the priming treatments that give positive effect on germination percentage, germination rate, seedling dry weight were priming treatment with KNO3 1.5%.


Author(s):  
Kranti M. Pawar ◽  
Prashant Kumar Rai

This present experiment was conducted in a Seed Testing laboratory, Department of Genetics and Plant Breeding SHUATS, Allahabad, UP during 2021 with bottle gourd, in order to standardize the best method of invigoration of priming specific to bottle gourd. method of priming viz., halopriming were evaluated by screening a different range of durations and chemical concentrations viz., T0 – Unprimed seeds (control), T₁ - 50 ppm KNO3 (Potassium Nitrate) for 12 hrs, T₂ - 50 ppm KNO3 for 18 hrs, T₃-100 ppm KNO3 for 12 hrs, T₄- 100 ppm KNO3 for 18 hrs, T₅-125 ppm KNO3 for 12 hrs, T₆- 125 ppm KNO3 for 18 hrs. Treated seeds were placed in between germination paper and in sand at control condition where data was subjected to factorial experiment laid out in completely randomised design (CRD). In which it was found that 6th treatment 125 ppm KNO3 for 18 hrs., has the significant result as compared to all the treatments with the control, the highest germination %, seedling length, weight and vigor index I & II. This study showed that Seed priming with KNO3 found to increase the seed quality parameters. In the present study proved market price- effective and most economical method. This study helps to improve the quality and performance of seeds with the help of seed priming treatments which have effect on market price and economic, non-toxic, eco-friendly sources.


2021 ◽  
Vol 3 (1) ◽  
Author(s):  
Awale Degewione Shirdon ◽  
Khalid Ibrahim Omer ◽  
R.P.S Tommer ◽  
Ketema Belete

Sorghum is being produced primarily as a food grain throughout the world. It is one of the major traditional food crops in Ethiopia. However, the productivity of the crop is very low and poor seed quality is one of the major determinants. An experiment study was conducted in laboratory and glasshouse to evaluate the effect of seed priming on seed quality parameters of three sorghum varieties:(Muyra-I, Muyra-II and Fendisha) with different storage of one year and two years using different priming agents with different soaking time for each priming agent (1.0mM KNO3, 1mM NaCl and distilled water for 72, 48 and 12 hours respectively) in 2012 at Haramaya University Seed laboratory. Completely randomized design in 2 x 3 x 4 factorial arrangement with three replications was used to carry out the germination and vigor tests. The priming concentrations and durations were based on previous work, which was effective in hydro and osmo-conditioning of sorghum seed. In the experiment, seed priming increased germination percentage (GP), Mean Germination Time (MGT) and improved seedling shoot (SSL) and root length (SRL), seedling dry weight (SDW) and seedling vigor indices in all varieties of both storage durations compared to their respective control. Priming with NaCl and KNO3 showed positive response followed by hydropriming for all varieties of different storage duration. When treated with sodium chloride, almost all the varieties of each storage year demonstrated an increase in germination and the degree of increment was higher for Muyra-I(__) and Fendisha (___), the lowest being for Muyra-II which was significantly lower for both storage year. In case of mean germination time Fendisha exhibited better with hydropriming for older seeds (___) and with KNO3 for newer seeds(___) while Muyra-II and Muyra-I showed better with KNO3 (____) for older seeds and with hydropriming for newer seeds. Responses of varieties for the remaining parameters were intermediate. Therefore, sorghum seed priming with osmo-media and water can compensate storage deterioration of sorghum seeds in addition to significantly improving the seed germination and vigor quality parameters 


2020 ◽  
Vol 12 (2) ◽  
pp. 84-87
Author(s):  
M Poovizhi ◽  
K Sujatha

The seeds must be viable and non-dormant for the efficient cultivation of the species of medicinal plants. The seeds of Solanum nigrum possess primary dormancy, which restricts germination. Hence, a laboratory experiment was conducted during 2019 in the Department of Seed Science and Technology, Agricultural College and Research Institute, Madurai, Tamil Nadu. The S. nigrum seeds were primed by soaking in different chemicals viz.,GA3 100ppm, Thiourea1%, KNO3 0.5%, Succinic acid 100 ppm, Ascorbic acid 100 ppm, hydro and dry control with soaking durations of 12h and seeds were dried under shade to bring back to their original moisture content and used for assessing the seed quality studies. The results revealed that among the different priming treatments Thiourea 1% recorded higher seed quality parameters viz, speed of germination (5.6), germination (88 %), seedling length (5.92 cm) and vigour index (520) and the enzyme activity of dehydrogenase (0.072 OD value) and lower values of electrical conductivity( 0.027 dsm-1),  amino acids (0.119 ?gg-1 ) and sugars ( 0.175 ?gg-1 ). Hence it could be recommended as pre-sowingg seed priming treatment in S. nigrum.


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