In vitro Allelopathic Effects of Plant Extracts on Germination of Vicia fabia (partim) Seeds

Author(s):  
S. Parafiniuk ◽  
E. Czerwiñska

The experiment evaluated the effect of plant extracts on germination and contamination of Vicia fabia seed “Bobas” variety of horse beans. The materials used for the studies were aqueous extracts made of 40 plant species. The studies aimed to indicate those plant species, which extracts limited their contamination, simultaneously having a stimulating impact on seeds germination. The germination capability of seeds stimulated extracts from green parts of Satureja hortensis, Zea mays stigmata, Verbascum thapsiforme flowers. The number of seeds colonised by microorganisms was limited by extracts from Zea mays stigmata, Taraxacum officinale roots, Urtica dioica leaves. The analysis of the germination capability of seeds and their contamination by germs showed that extracts prepared in the form of brew had the most favourable effect. All forms of extracts from green parts of Artemisia vulgaris,Taraxacum officinale roots,Urtica dioica leaves had the most favourable effect on germination of horse bean seeds and at the same time on limitation of their contamination.

2017 ◽  
Vol 13 (1) ◽  
pp. 56-59
Author(s):  
Aura M. Blandón ◽  
Oscar M. Mosquera ◽  
Antônio E. G. Sant’ana ◽  
Aldenir F. Dos Santos ◽  
Luana L. S. Pires

The present study describes the in vitro antioxidant activity of methanol extracts of 34 plant species collected in the Colombian coffee-growing eco-region belonging to Euphorbiaceae, Piperaceae and Solanaceae families. The antioxidant properties of extracts were evaluated by determining radical scavenging power measured with a DPPH assay. The methanolic extracts of  Hyeronimia antioquiensis, Mabea montana, and Alchornea grandis species (Euphorbiaceae), presents EC50 values equal to 0.686, 12.35, and 13.01 µg/mL, respectively, showing high antioxidant potential.


2021 ◽  
Vol 71 (3) ◽  
pp. 429-443 ◽  
Author(s):  
Alona Savych ◽  
Svitlana Marchyshyn ◽  
Ivanna Milian

AbstractDue to the wide range of biologically active substances, the herbal mixtures can influence the development of diabetes mellitus and its complications. Carbohydrates attract particular attention due to their hypoglycemic, hypolipidemic, anticholesterolemic, antioxidant, antiinflammatory and detoxifying activities. The aim of this study was to investigate the content of carbohydrates through their monomeric composition in the herbal mixture samples: a) Urtica dioica leaf, Cichorium intybus roots, Rosa majalis fruits, Elymys repens rhizome, Taraxacum officinale roots, b) Arctium lappa roots, Elymys repens rhizome, Zea mays columns with stigmas, Helichrysum arenarium flowers, Rosa majalis fruits, c) Inula helenium rhizome with roots, Helichrysi arenarium flowers, Zea mays columns with stigmas, Origanum vulgare herb, Rosa majalis fruits, Taraxacum officinale roots, d) Cichorium intybus roots, Elymys repens rhizome, Helichrysum arenarium flowers, Rosa majalis fruits, Zea mays columns with stigmas and e) Urtica dioica leaf, Taraxacum officinale roots, Vaccinium myrtillus leaf, Rosa majalis fruits, Mentha piperita herb, which were used in Ukrainian folk medicine for the prevention and treatment of diabetes mellitus type 2.The carbohydrates were separated by gas chromatography-mass spectrometry after conversion into volatile aldononitrile acetate derivatives. The monomeric composition of polysaccharides was studied after their hydrolysis to form monosaccharides and poly-alcohols.Quantitative analyses of free carbohydrates showed that the predominant sugars were fructose, glucose and disaccharide – sucrose, in all samples. Concerning the determination of polysaccharide monomers after hydrolysis, glucose was the most abundant in all samples. The chromatographic study revealed a number of polyalcohols that are important for the treatment and prevention of progression of diabetes mellitus and its complications, namely, mannitol, pinitol and myo-inositol.


2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Hirut Basha ◽  
Hassen Mamo

Background. Schistosoma mansoni remains a significant health problem in low-income countries. Praziquantel (PZQ) is the only drug available to treat schistosomiasis, and PZQ resistance is a potential threat towards control of the disease although PZQ is currently effective against all species of schistosomes. Moreover, PZQ is less efficacious against larval stages. In response to these challenges, multiple in vivo/in vitro studies evaluated the anti-S. mansoni activity of crude plant extracts in a bid for novel drug(s). However, these studies appear fragmented and patchy. This systematic review explored the extent of such studies in the past 11 years (2010-2020). Methods. A systematic web search analysis and review of the literature on crude plant extracts tested against S. mansoni was done. Data from 17 articles meeting eligibility criteria were extracted and analyzed. Forty-three plant species have been tested by the 17 studies. The leaves, barks, stems, flowers, rhizomes, and roots of the plants as well as the whole plant part were used for the experiments. Conclusion. Nearly all of the plants significantly reduced schistosome egg output, killed adult worms, and improved liver histology and function. Further studies are required to assess the therapeutic potential of more promising plant species.


Nematology ◽  
2010 ◽  
Vol 12 (1) ◽  
pp. 1-24 ◽  
Author(s):  
Michael Boppré ◽  
Tim Thoden

Abstract1,2-dehydropyrrolizidine ester alkaloids (pyrrolizidine alkaloids; PAs) are a class of secondary plant metabolites found in hundreds of plant species. In vitro, PAs are known to affect plant-parasitic nematodes. Thus, PA-producing plants might be used in nematode management. So far, however, Crotalaria is the only PA-producing plant that has been used for nematode control. Consequently, we reviewed the literature on other PA plants and their effects on nematodes. This included the analysis of in vitro experiments with PA plant extracts, host tests with PA plants, as well as experiments in which PA-producing plants were used as different types of soil amendments (mulch, in situ mulching, extracts). Our results clearly show that, in addition to Crotalaria, certain other PA-producing plant species have the potential to be used in sustainable nematode management. This is especially true for management of sedentary endoparasitic nematode species. Promising applications include the use of PA plant extracts and mulches, as well as the use of PA plants for in situ mulching.


Author(s):  
María R. Flores-Ocelotl ◽  
Nora H. Rosas-Murrieta ◽  
Diego A. Moreno ◽  
Verónica Vallejo-Ruiz ◽  
Julio Reyes-Leyva ◽  
...  

Molecules ◽  
2020 ◽  
Vol 25 (10) ◽  
pp. 2434
Author(s):  
Eun Kyeong Lee ◽  
Jeongah Song ◽  
Youjin Seo ◽  
Eun Mi Koh ◽  
Seon-Hee Kim ◽  
...  

The purpose of this study was to determine the antiallergic effects of AF-343, a mixture of natural plant extracts from Cassia tora L., Ulmus pumila L., and Taraxacum officinale, on rat basophilic leukemia (RBL-2H3) cells. The inhibitory effects on cell degranulation, proinflammatory cytokine secretion, and reactive oxygen species (ROS) production were studied in compound 48/80-treated RBL-2H3 cells. The bioactive compounds in AF-343 were also identified by HPLC–UV. AF-343 was found to effectively suppress compound 48/80-induced β-hexosaminidase release, and interleukin (IL)-4 and tumor necrosis factor-α (TNF-α) production in RBL-2H3 cells. In addition, AF-343 exhibited DPPH free radical scavenging effects in vitro (half-maximal inhibitory concentration (IC50) = 105 μg/mL) and potently inhibited compound 48/80-induced cellular ROS generation in a 2′,7′-dichlorofluorescein diacetate (DCFH-DA) assay. Specifically, treatment with AF-343 exerted stronger antioxidant effects in vitro and antiallergic effects in cells than treatment with three single natural plant extracts. Furthermore, AF-343 was observed to contain bioactive compounds, including catechin, aurantio-obtusin, and chicoric acid, which have been reported to elicit antiallergic responses. This study reveals that AF-343 attenuates allergic responses via suppression of β-hexosaminidase release, IL-4 and TNF-α secretion, and ROS generation, perhaps through mechanisms related to catechin, aurantio-obtusin, and chicoric acid. The results indicate that AF-343 can be considered a treatment for various allergic diseases.


2020 ◽  
pp. 3-9
Author(s):  
Бекетова О.А. ◽  
Комаров И.В.

В статье представлены результаты исследования сорной флоры Енисейского и Казачинского районов Красноярского края. Объектом исследования является сорный компонент флоры Красноярского края. Цель исследования: обследовать растительные сообщества рудеральных и нарушенных местообитаний, выявить наиболее распространенные виды в условиях тайги и подтайги земледельческой части Красноярского края. Маршрут прoхoдил вдoль oснoвнoй трассы Краснoярск – Енисейск (04К-044 «Енисейский тракт»), oхват примернo oт 170 до 284 км. При oбследoвании делали oстанoвки через каждые 8–10 км в зависимoсти oт наличия участкoв, занятых пoд зернoвые культуры. На местообитаниях разного типа наиболее представительными являются семейство Poaceae Bernhart. Мятликовые, семейство Asteraceae Dumort. Астровые, также больше видов семейства Fabaceae Lindl. Бобовые и семейства Brassicaceae Burnett (Cruciferae Juss.) Капустные. В посевах зерновых культур более обильно и чаще встречаются следующие виды: Apera spica-venti (L.) Beauv. – метлица обыкновенная, Equisetum arvense L. – хвощ полевой, Amaranthus retroflexus L. – щирица запрокинутая, Taraxacum officinale Wigg. – одуванчик лекарственный, Sonchus arvensis L. – осот полевой, Cichorium intybus L. – цикорий обыкновенный, Cannabis ruderalis Janish – конопля сорная. Для каждого растения на нарушенных местообитаниях рассчитали: встречаемость, средний балл обилия вида, интегральный индекс встречаемости и обилия. Наиболее высокий интегральный индекс выявлен для видов: Elytrigia repens (L.) Nevski – пырей ползучий, Cirsium setosum (Willd.) Bess. – бодяк щетинистый, осот розовый, Equisetum arvense L. – хвощ полевой, Artemisia vulgaris L. – полынь обыкновенная, чернобыльник, Matricaria perforate Меrat – ромашка непахучая, Anthriscus sylvestris – купырь лесной, морковник, дудка, Trifolium repens L. – клевер ползучий, Plantago major L. – подорожник большой, Festuca pratensis Huds. – овсяница луговая, Urtica dioica L. – крапива двудомная, который составляет 1,24 – 0,41. На рудеральных местообитаниях наиболее вредоносные виды: Elytrigia repens (L.) Nevski – пырей ползучий, Cirsium setosum (Willd.) Bess. – бодяк щетинистый, осот розовый, Equisetum arvense L. – хвощ полевой, Artemisia vulgaris L. – полынь обыкновенная, чернобыльник.


2020 ◽  
Vol 20 (5) ◽  
pp. 620-650
Author(s):  
Tooran Nayeri Chegeni ◽  
Mahdi Fakhar ◽  
Fatemeh Ghaffarifar ◽  
Reza Saberi

Background: Recently, herbal medicine has received much attention in the literature. Several essential oils or plant extracts have been found to have anti-Acanthamoeba properties against trophozoites and cysts of Acanthamoeba spp. Objective: The aim of this systematic review is to introduce anti-Acanthamoeba properties of some essential oils or plant extracts; perhaps the results of this research will be used to prevent and treat infectious diseases. Methods: All published papers in English and Persian databases were systematically searched for some specific keywords to find articles that have influenced plant compounds on Acanthamoeba up to April 2018. Articles related to the subject were selected and studied. Results: A total of 51 articles including 136 experiments (128 in vitro and 8 in vivo) between 1999 and 2018, met our eligibility criteria. Totally, 110 species of plants belonging to 34 families, mainly Lamiaceae (15 plant species) and Asteraceae (13 plant species) were studied against trophozoites and cysts of Acanthamoba in in vitro and in vivo. Most of the plant's species were Citrus (6 species), Allium (5 species), Peucedanum, Piper, Lippia, and Olive (4 species). The most frequently used parts were leaves, aerial parts, flowers, bark, rhizomes, and seeds. Conclusions: Recent studies have shown that many natural compounds have high anti-parasitic properties and low toxicity. Our research team hopes that the information provided in present systematic review can improve new experimental and clinical trials and herbal combination therapy. Further studies are needed to understand the molecular mechanisms in the anti-amoebic reactions of plant species and ocular toxicity of extracts in animal models.


Planta Medica ◽  
2009 ◽  
Vol 75 (09) ◽  
Author(s):  
WR Cunha ◽  
FM Dos Santos ◽  
JA Peixoto ◽  
RCS Veneziani ◽  
AEM Crotti ◽  
...  

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