Comparative Studies on Mutagenic Effectiveness and Efficiency in Horsegram [Macrotyloma uniflorum (Lam) Verdc.]

2020 ◽  
Author(s):  
S. Priyanka ◽  
R. Sudhagar ◽  
C. Vanniarajan ◽  
K. Ganesamurthy ◽  
J. Souframanien ◽  
...  
Keyword(s):  
Gamma Rays ◽  
Wide Spectrum ◽  
Block Design ◽  
Seed Fertility ◽  
Combination Treatments ◽  
Macrotyloma Uniflorum ◽  
Randomized Block Design ◽  
Methane Sulphonate ◽  
Chlorophyll Mutants ◽  
Effectiveness And Efficiency

Background: Two horsegram varieties viz., PAIYUR 2 and CRIDA1-18R were mutated with gamma rays (G), electron beam (EB), G+EB and G+EMS (ethyl methane sulphonate) to determine the mutagenic potency in breeding programme.Methods: Uniform seeds treated with different mutagenic doses were raised in randomized block design which constituted M1 generation. Each plant was harvested individually and forwarded to M2 generation following plant to progeny row method.Result: A dose dependant decline was observed for seed germination, plant survival, root length, shoot length, plant height, pollen fertility and seed fertility in M1 population. Wide spectrum of chlorophyll mutants was induced in M2 generation with maximum frequency at EB followed by combination treatments (G+EMS and G+EB) in both varieties. The mutagenic effectiveness ranged from 0.14 per cent to 1.45 per cent in PAIYUR 2 and 0.15 per cent to 1.71 per cent in CRIDA1-18R. The high mutation rate for effectiveness was exhibited by G and G+EB. With reference to sterility, EB was found to be efficient mutagen in both varieties whereas varied efficiency was noted for lethality (EB - PAIYUR 2; G+EMS - CRIDA1-18R) and injury (G - PAIYUR 2; G+EMS - CRIDA1-18R). 

scholarly journals Studies on Mutagenic Effectiveness and Efficiency of Gamma Rays and Ethyl Methane Sulphonate in Jasmine

2019 ◽  
pp. 1-8
Author(s):  
Sanchita Ghosh ◽  
M. Ganga ◽  
K. Soorianathasundaram
Keyword(s):  
Gamma Rays ◽  
High Frequency ◽  
Wide Spectrum ◽  
Ethyl Methane Sulphonate ◽  
Biological Damage ◽  
Flower Yield ◽  
Methane Sulphonate ◽  
Chlorophyll Mutants ◽  
Effectiveness And Efficiency ◽  
Morphological Mutation

Background/Aim: The present investigation was undertaken to study the mutagenic effectiveness and efficiency in M1V1 generation and to study effect of gamma rays on spectrum of morphological mutation in Jasminum grandiflorum Linn. cv. White Pitchi. Methods: Terminal cuttings were treated with four doses of gamma rays viz., 10, 15, 20 and 25 Gy and four doses of EMS viz., 25, 30, 35 and 40 mM separately. Both mutagens created a high frequency as well as a wide spectrum of mutation. Results: Totally five types of chlorophyll mutants viz., xantha, viridis, yellow viridis, variegata and tigrina were observed. The mutagenic effectiveness and efficiency were calculated based on biological damage as well as chlorophyll mutation frequency on M1 plants. The mutagenic treatments were effective in inducing various types of morphological macro mutants, with few of them showing significant changes in plant height, flowering parameters and flower yield. The lower mutagen doses were associated with higher mutagenic effectiveness and efficiency. Conclusion: The present study indicated that the physical mutagen gamma rays were more effective and efficient in causing mutations as compared to the chemical mutagen EMS.

scholarly journals Effect of mutagenic treatments on development of mutants in turmeric cv. Prathibha (Curcuma longa L.)

2021 ◽  
Vol 17 (AAEBSSD) ◽  
pp. 116-120
Author(s):  
M. Lakshmi Madhuri ◽  
M.L.N. Reddy ◽  
K. Giridhar ◽  
N. Ratna Kumari
Keyword(s):  
Open Field ◽  
Gamma Rays ◽  
Present Experiment ◽  
Mutation Frequency ◽  
Curcuma Longa ◽  
Block Design ◽  
Randomized Block Design ◽  
Effectiveness And Efficiency ◽  
The Impact ◽  
Mutagenic Efficiency

The present experiment was carried out during Kharif, 2016-2017 at College of Horticulture, Dr. Y.S.R. Horticultural University, Venkataramannagudem to assess the impact of mutagens on mutagenicity like Mutation frequency on M1 population basis, Mutagenic effectiveness, Mutagenic efficiency and Frequency of albino, xantha and chlorina mutants in turmeric. The experiment was laid out in factorial randomized block design and replicated thrice under open field conditions. Rhizomes are treated with gamma rays (25 Gy and 50 Gy) and EMS (250 ppm, 500 ppm and 1000 ppm) along with control. Maximum mutagenic frequency, effectiveness and efficiency was observed in25 Gy, 250 ppm EMS and combination of 25 Gy + 250 ppm EMS and minimum was recorded in 50 Gy gamma rays, 1000 ppm EMS and combination both 50 Gy + 500 ppm EMS and 50 Gy + 1000 ppm EMS .

Field Efficiency of Certain Biopesticides and Neem Products Against Helicoverpa armigera (Hubner) on Chickpea (Cicer arietinumL.)

2019 ◽  
Vol 10 (01) ◽  
Author(s):  
Nihad H. Mutlag ◽  
Ameer S. A. Al-Haddad
Keyword(s):  
Helicoverpa Armigera ◽  
Block Design ◽  
Individual Treatment ◽  
Neem Oil ◽  
Larval Population ◽  
Neem Cake ◽  
Combination Treatments ◽  
Randomized Block Design ◽  
Helicoverpa Armígera ◽  
Nuclear Polyhedrosis

A field study was conducted to evaluate the efficiency of four microbial insecticides viz. Beauveria bassiana; HaNPV (Helicoverpa armigera Nuclear Polyhedrosis virus); (Bacillus thuringiensisvar.kurstaki 2 gm/L); HaNPV+Bt; neem oil; neem cake and D.D.V.P EC 76% @0.05% at Research Farm SHIATS,Allahabad during rabi season of 2011-2012. The experiment was laid out in randomized block design with seven treatment and replicated thrice. The observation larval populations of H. armigera were recorded one day before treatment was recorded at 3,7, and 10 days after treatments. The larva population of H. armigera appeared in the third week of February (8 the Standard week) and reached its peak of 14.65 larvae in first week of April and decline rapidly with maturation of crop. There was only one peak in the larval population observation in the 1st week. Bacillus thuringiensis was the most effective chemical by D.D.V.P.76%@0.05% . Among the microbial insecticides. HaNPV ,was the most effective followed by HaNPV+Bt and neem cake . The combination treatments were less effective than the individual treatment neem oil and B. bassiana were the least effective treatment in reducing the larval population of Heliverpa armigera.

PENGARUH POPULASI TANAMAN DAN PENGGUNAAN MULSA TERHADAP POPULASI GULMA DAN HASIL PUCIL JAGUNG MANIS (ZEA MAYS SACCHARATA STURT) DI LAHAN KERING

AGRICA ◽  
2020 ◽  
Vol 6 (2) ◽  
pp. 124-133
Author(s):  
I Ketut Arsa Wijaya
Keyword(s):  
Zea Mays ◽  
Block Design ◽  
Plant Population ◽  
Cyperus Rotundus ◽  
Corn Kernel ◽  
Plastic Mulch ◽  
Rice Plants ◽  
Straw Mulch ◽  
Combination Treatments ◽  
Randomized Block Design

This study was conducted in Balai Benih Induk (BBI) Luwus, Baturiti District, Tabanan Regency, with the high of place 506m above sea level occurring in March to May 2014. This research used randomized block design arranged per factor, namely population of plants (P) including three steps: P1=80.000 plants per hectare, P2= 100.00 plants per hectare, P3= 120.000 per hectare; and mulch (M) that include: M0= without mulch and without cleaning, M1= without mulch with clean weeding, M2= rice plants straw mulch (5 ton per hectare) and M3= black silver plastic mulch. Eventually, there were 12 combination treatments, each of which was repeated 3 times to obtain 36 land slots. The research indicates that there was no real interaction between the treatment of plant population and mulch usage in all variables observed. The 100.000 plant population per hectare can produce the weight of dry and fresh oven corn kernel without highest cornhusk that was to say 4.79 tons and 0.53 tons or 11.66 % and 20.45% higher compared to 120.000 plant population per hectare. The use of black silver plastic and rice plants straw mulch can produce the weight of fresh and dry oven corn kernel without cornhusk per hectare each of which is 4.69 tons, 4.57 tons, and 0.54 tons, 0.47 tons or 26.76%, 38.46 %, and 23.51 %, 20.51 % higher than without mulch and without clean weeding. Clean weeding treatment can produce the weight of fresh and dry oven corn kernel without cornhusk per hectare with its weight of 4.63 tons and 0.51 tons or 25.35 % and 35.90 % higher than without mulch and cleaning. Clean weeding treatment was not obviously different from mulch treatment. The identification of weed types were 19 kinds of weeds and Cyperus rotundus L. Weeds. They have the highest density and each absolute frequent of 1249.333 stems m-2 and 1.00.

scholarly journals PEMUPUKAN NITROGEN, FOSFOR, DAN KALIUM PADA TANAMAN AKAR WANGI

2020 ◽  
Vol 19 (1) ◽  
pp. 33
Author(s):  
ROSIHAN ROSMAN ◽  
OCTIVIA TRISILAWATI ◽  
SETIAWAN SETIAWAN
Keyword(s):  
Key Words ◽  
Block Design ◽  
Vetiveria Zizanioides ◽  
Optimum Dosage ◽  
Combination Treatments ◽  
Randomized Block Design ◽  
K Fertilizer ◽  
Increase Productivity ◽  
It Usage

<p>ABSTRAK<br />Dosis pupuk N, P, dan K optimal untuk akar wangi belum diketahui<br />dan penggunaannya  masih beragam. Penelitian bertujuan untuk<br />mendapatkan komposisi dosis pupuk N, P, dan K optimal yang dapat<br />meningkatkan produktivitas akar wangi. Penelitian dilakukan di Desa<br />Sukakarya, Garut dari bulan Januari 2009 sampai dengan Desember 2010<br />menggunakan rancangan Acak Kelompok, dengan 3 ulangan. Perlakuan<br />meliputi 9 kombinasi pupuk N, P, dan K: (1). Kontrol; (2) 100 kg SP-36 +<br />75 kg KCl; (3) 100 kg ZA + 75 kg KCl; (4) 100 kg ZA + 50 kg SP-36 + 75<br />kg KCl; (5) 100 kg ZA + 100 kg SP-36 + 75 kg KCl; (6) 100 kg ZA + 100<br />kg SP-36 + 150 kg KCl; (7) 100 kg ZA + 100 kg SP-36; (8) 200 kg ZA +<br />100 kg SP-36 + 75 kg KCl; (9) 200 kg ZA + 100 kg SP-36 + 150 kg KCl.<br />Panen dilakukan pada 12, 14, dan 16 bulan setelah tanam (BST). Hasil<br />menunjukkan bahwa pemupukan dosis 100 kg ZA + 75 kg KCl<br />menghasilkan minyak 52,59 dan 67,78 kg/ha (12 dan 14 BST) dan 200 kg<br />ZA + 100 kg SP-36 + 75 kg KCl menghasilkan 67,76 kg /ha (16 BST),<br />dengan kadar vetiverol lebih dari 50%.<br />Kata kunci: Vetiveria zizanioides, pemupukan, vetiverol, produksi, mutu<br />minyak</p><p>ABSTRACT<br />The optimum dosage of N, P, and K fertilizer has not been known<br />yet and it usage was still varied. The research aim is to obtain an optimal<br />composition of N, P, and K fertilizer that could increase productivity of<br />vetiver crop. The researsch has been conducted in Sukakarya Village,<br />Garut, from January 2009 to December 2010. The research was arranged<br />in randomized block design, with 3 replications and N, P, and K fertilizer<br />combination treatments i.e.: (1) Control; (2) 100 kg SP-36 + 75 kg KCl;<br />(3) 100 kg ZA + 75 kg KCl; (4) 100 kg ZA + 50 kg SP-36 + 75 kg KCl;<br />(5) 100 kg ZA + 100 kg SP-36 + 75 kg KCl; (6) 100 kg ZA + 100 kg SP-<br />36 + 150 kg KCl; (7) 100 kg ZA + 100 kg SP-36; (8) 200 kg ZA + 100 kg<br />SP-36 + 75 kg KCl; (9) 200 kg ZA + 100 kg SP-36 + 150 kg KCl.<br />Harvesting was done at 12, 14 and 16 months after planting (MAP). The<br />result showed that the dose of 100 kg ZA + 75 kg KCl produced vetiver oil<br />52,59 and 67,78 kg/ha (12 and 14 MAP). Meanwhile the dose of 200 kg<br />ZA + 100 kg SP-36 + 75 kg KCl produced 67,76 kg/ha (16 MAP),<br />respectively. vetiverol content were more than 50%.<br />Key words: Vetiveria zizanioides, fertilizing, vetiverol, production, oil<br />quality</p>

Induced mutagenic effectiveness and efficiency studies on Pigeon pea (Cajanus cajan (L.) Millsp).

Food Biology ◽  
2016 ◽  
Vol 4 ◽  
Author(s):  
M. Ariraman ◽  
T. Bharathi ◽  
D. Dhanavel
Keyword(s):  
Gamma Rays ◽  
Cajanus Cajan ◽  
Relative Effectiveness ◽  
Pigeon Pea ◽  
High Yield ◽  
Early Maturity ◽  
Biological Damage ◽  
Chlorophyll Mutants ◽  
Late Maturity ◽  
Effectiveness And Efficiency

<p>The present investigation was carried out to study mutagenic effectiveness and efficiency of gamma rays and EMS treatments in Pigeon pea (Cajanus  cajan (L.) Millsp) Var CO-7. The relative effectiveness and efficiency of the both mutagen used was assessed from the data on biological damage in M1 generation and frequency of chlorophyll and viable mutants in M2 generation. The spectrum of chlorophyll mutants such as xantha, albino, chlorina and viridis, viable mutants like tall, dwarf, early flower, early maturity, late maturity, bushy, high yield and seed mutants were observed in both the mutagenic treatments.  Among the chlorophyll mutants xantha was found more in number. The mutagenic effectiveness and efficiency were found to be higher at 20KR of gamma irradiation and 25mM of EMS. The mutation rate of gamma rays was higher in terms of effectiveness than that of EMS. More number of chlorophyll and viable mutants was induced in gamma rays treatment when compared EMS treatment.</p>

scholarly journals Induced Mutagenesis in Cicer arietinum

2014 ◽  
Vol 12 ◽  
pp. 1-4 ◽  
Author(s):  
L. Mullainathan ◽  
S. Umavathi
Keyword(s):  
Cicer Arietinum ◽  
Gamma Rays ◽  
Crop Improvement ◽  
Ethyl Methane Sulphonate ◽  
Chick Pea ◽  
Biological Damage ◽  
Methane Sulphonate ◽  
Dose Concentration ◽  
Induced Mutagenesis ◽  
Effectiveness And Efficiency

The traditional varieties of chick pea have low potentiality and restricted variability with respect to economic characters. Broadening the genetic base for crop improvement can be quickly achieved through induced mutagenesis. The present study was undertaken in order to comparing the effectiveness and efficiency of mutagens on Cicer arietinum. In this regard, Co – 4 variety of chick pea was subjected to different dose/concentration of Gamma rays (20, 30, 40, 50 and 60 kR) and Ethyl Methane Sulphonate (10, 20, 30, 40 and 50 mM) for inducing mutation. Mutagenic effectiveness and efficiency was calculated based on biological damage in M1 and chlorophyll mutations in M2. The results indicated that, mutagenic effectiveness increased with the increase in dose/concentration of mutagen. Intermediate treatments in general were found more efficient in causing less biological damage and inducing maximum amount of mutations. It shows that the chemical mutagens are more effective and efficient than physical mutagen for inducing mutation in Chick pea

scholarly journals Efforts to produce Siamese citrus fruit out of season and fruit quality improvement through application of potassium nitrate and agrodyke fertilizer

2018 ◽  
Vol 2 (3) ◽  
pp. 48-58
Author(s):  
Ni Komang Alit Astiari ◽  
Luh Kartini ◽  
Ni Putu Anom Sulistiawati ◽  
I Nyoman Rai
Keyword(s):  
Block Design ◽  
Organic Fertilizer ◽  
Citrus Fruit ◽  
Potassium Nitrate ◽  
Fertilizer Treatment ◽  
Nitrate Treatment ◽  
Combination Treatments ◽  
Randomized Block Design ◽  
Orange Fruit

The research aimed to find out the efforts to produce off-season orange fruit and increase the quality of fruit through the application of potassium nitrate and agrodyke organic fertilizer. The study used a factorial randomized block design with 2 treatment factors. The first factor is the flower induction application using potassium nitrate (K) consisting of 3 levels, namely (K0 = 0 g/tree), (K1 = 20 g/tree), and (K2 = 40 g/tree). While the second factor is the application of agrodyke organic fertilizer (P) consisting of 4 levels, namely (P0 = 0 g / tree), (P1 = 15 g/tree), (P2 = 30 g / tree) and (P3 = 45 g/tree). There are 12 combination treatments and each of them is repeated 3 times so that 36 tree plants are needed. The results showed the interaction between potassium nitrate treatment with agrodyke organic fertilizer treatment had no significant effect on all observed variables. Potassium nitrate treatment and agrodyke organic fertilizer can significantly improve the quality of orange citrus fruit out of season physically. In the treatment of potassium nitrate 40 g/tree, the weight of the harvested fruit per tree was obtained, the weight per fruit and the highest fruit diameter were 13703.90 g; 99.92 g and 6.81 cm or an increase of 62.24%; 16.32% and 9.49% compared with no treatment of potassium nitrate which is 8446.60 g; 87, 42 g and 6.22 cm.

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