Isolation of Gamma Ray Induced Urd Bean [Vigna mungo (L.) hepper] Mutants with Improved Batter Quality

Author(s):  
C. Vanniarajan ◽  
J. Souframanien ◽  
S. Anandhi Lavanya

Background: The urd bean variety MDU 1 has a duration of 70-75 days, bushy in nature and is susceptible to yellow mosaic virus. In order to develop a determinate type and improved batter quality, MDU 1 and VBN (Bg) 4 blackgram seeds were treated with different doses (100 Gy, 200 Gy, 300 Gy, 400 Gy and 500 Gy) of gamma rays. Methods: Uniform sized seeds treated with different mutagenic doses were raised in randomised block design which constitute M1 generation. Each plant was harvested individually and forwarded to M2 generation following plant to progeny row method. The determinate types were selected from M2 generation and forwarded to further generations. After attaining homozygosity in the advanced generation, the mutants were checked for its biochemical characters (Total solubule protein content analysed by kjeldahl method, albumin and globulin analysed by Lowery’s method, arabinose content analysed by Bial method. Result: The mutants ACM - 16 -011, ACM - 16 -015, ACM - 16 -018 were found to have an arabinose content of 8.28%, 8.98% and 8.14% respectively. All these mutants recorded more batter volume over the variety MDU 1. The albumin (%) and globulin (%) contents were also found at remarkably increased levels in the mutants. These mutants have the potential to develop a high quality variety of urd bean and therefore are very useful in breeding programme.

2021 ◽  
Vol 6 (1) ◽  
pp. 7
Author(s):  
Yukarie Ayu Wulandari ◽  
Sobir Sobir ◽  
Syarifah Iis Aisyah

Cowpea (V. unguiculata L) can be developed as a source of vegetable protein because it contains relatively high protein and high lysine. The protein content in seeds is an accumulation of character components that play a role in protein formation, whose relationship can be predicted using cross correlation and analysis coefficients. The study was carried out in the experimental garden of Pasir Kuda PKHT of IPB in February - May 2018. Protein analysis is carriedout in the Testing Laboratory of the Department of Agronomy and Horticulture, Faculty of Agriculture, IPB in June - July 2018. The study was conducted using 30 putative mutant test genotypes of M2 generation result from the mutation of gamma ray irradiation and KM4 genotype as a comparison which was repeated three times. The protein content of the seeds was analyzed using the Kjeldahl method and then analyzed the variance, correlation coefficient and path. The results showed that M2 generation putative mutants showed diversity for the character of protein content in cowpea seeds. The protein content in cowpea seeds can be predicted through the character of plant height and number of branches.


2020 ◽  
Vol 36 (1) ◽  
pp. 97
Author(s):  
Nandariyah Nandariyah ◽  
Endang Yuniastuti ◽  
Sukaya Sukaya ◽  
Sonia Ika Yudhita

<p><a name="_Hlk39513249"></a><span lang="EN-US">Raja Bulu is one of the banana varieties favored by the community because of its thick fruit flesh and sweet taste. However, its parthenocarpic characteristic and vegetative propagation make this banana variety has limited genetic variation. Attempt to improve the genetic variation was conducted through induced mutation breeding using gamma-ray mutagens. This research aimed to select M1V1 generation of Raja Bulu banana (<em>Musa paradisiaca</em> Linn.) obtained by gamma rays’ irradiation for their growth traits which are expected to produce banana varieties that have an early maturity and high yield. This study used a randomized complete block design without replication by observing the generative growth of each individual of Raja Bulu banana irradiated by gamma rays and without radiation as a control. The results showed that gamma-ray irradiation treatment caused Raja Bulu banana to be harvested earlier and produced higher fruit weight than controls. The gamma-ray irradiation had a random influence on Raja Bulu bananas. The 10 Gy gamma-ray irradiation dosage influenced the morphological diversity in the generative phase of Raja Bulu banana. The treatment of gamma irradiation resulted in 5 individual plants that flowered and matured earlier as compared to controls</span><span lang="IN">.</span></p>


2017 ◽  
Vol 2 (2) ◽  
pp. 070
Author(s):  
Amri Amanah ◽  
Sri Nuryani Hidayah Utami ◽  
Makruf Nuruddin

The objective of this study was to examine the effect of planting distance of paddy var. Rojolele planted in conventional legowo system and dosage of gamma rays irradiated on nitrogen uptake and productivity of paddy. The experiment was arranged in a randomized complete block design (RCBD) with twelve treatments and three replications. The first factor was gamma ray radiation, consisted of three levels, namely control seed (B0), seed irradiated with gamma rays of 200 gray (B2), and seed irradiated with gamma ray of 300 gray (B3). The second factor was planting distance, consisted of four levels, namely distance of 25 x 25 (J1), distance of 30 x 30 (J2), legowo system of 2 : 1 (J3), and legowo system of 4 : 1 (J4 ). The results showed that jajar legowo system with planting distance of 2 : 1 significantly affected the nutrient content of nitrogen in shoot, nitrogen uptake of plant, and yield  per hectare. Paddy var. Rojolele irradiated by 300 gray produced higher nitrogen content in shoot, nitrogen uptake of plant, and yield per hectare than those in control seeds and seeds irradiated with 200 gray. There was a tendency that planting distance with jajar legowo system resulted in higher grain (yield), on the contrary, the narrow row spacing impacted on lower grain (yield).


2020 ◽  
Vol 04 (05) ◽  
pp. 40-43
Author(s):  
Ilhama Ibrahim Rzayeva ◽  

The effect of different doses of gamma rays of the Co60 isotope on the seeds of cotton varieties AzNIXI-104 and AzNIXI-195 before sowing caused changes in the growing season, plant height, sympodial branches and the number of bolls on the bush. At low doses of gamma rays (500 r, 5000 r) the vegetation period was shortened, the number of sympodial branches and bolls in the branch increased, and at high doses of mutagen a decrease in biomorphological features was observed. Key words: cotton, sort, gamma ray, mutagen, sympodial branch, vegetation period.


2019 ◽  
pp. 19-22
Author(s):  
Pandiyan M ◽  
Senthil N ◽  
Sivakumar C ◽  
Krishnaveni A ◽  
Vaithiyalingan M

The green gram culture VGG04-001 is developed from a cross derivative of K 1 x Vellore local released as VBN (Gg)3 maturing in 65-70 days and suited for cultivation under both under rainfed and irrigated conditions. It has a yield potential of 826 Kg per hectare. It is multiblooming type with moderately resistance to Yellow Mosaic Virus and Powdery Mildew. It possesses desirable characters like high protein content (24.16%). It is recommended for cultivation in Tamil Nadu except Nilgiris and Kanyakumari districts of Tamil Nadu. Keywords: VGG04-001; Green gram; Yellow Mosaic Virus; Powdery Mildew; Rainfed; Irrigated


Author(s):  
B. Rajitha ◽  
V. Rajarajeswari ◽  
P. Sudhakar

The present field study was conducted to characterize the blackgram genotypes based on morpho and nutrient content under yellow mosaic virus disease infection. The present investigation was carried out with ten blackgram genotypes (5 known tolerant + 5 known susceptible) in Randomized Block Design (RBD) during summer 2018. Experimental crop was affected by yellow mosaic virus (YMV) naturally at 30 DAS. No management practices were taken for controlling of whitefly population during crop season. Correlation studies were done at 50 DAS which helps to find out the most resistant and most susceptible genotypes among ten backgram genotypes based on leaf pubescence and nutrient content in leaf. Leaf pubescence is one of the most important resistant factors in a number of crops and Minerals, apart from being a vital part of the plant nutrition, may manifest certain maladies in the plants either through disturbing normal metabolism and physiology of the plants or by favouring or by discouraging the plant pathogens, if in excess or otherwise deficient. Correlation analysis of present field investigation revealed that blackgram genotypes showed strong negative correlation for leaf pubescence, leaf Phosphorous (P) and leaf potassium (K) content with percentage disease index (PDI%) as well as strong positive correlation for leaf Nitrogen (N), leaf Iron (Fe) and leaf Zinc (Zn) content under YMV.


Author(s):  
İlknur Akgün ◽  
Tuğçe Ayşe Karakoca ◽  
Ruziye Karaman

In this study, the effect of different doses of gamma ray (200, 300, 400 and 500 Gy) applied on seeds of Tarm-92 two-row barley varieties on some agricultural properties were investigated. The research was conducted in 2016-2017 vegetation period under Isparta conditions. In M1 generation, normal and abnormal plants are harvested separately according to the spike and plant appearance at each dose and 9 different [200, 200 A, 300, 300 A, 400, 400 A, 400 A (plants with double spike formation and branching tendency in spike Ç.B.) 500 and 500 A gray] groups were formed. A total of 10 plots with control (no irradiation) were included in the M2 generation. Results showed that the effect of gamma ray application on the thousand grain weight, test weight, ratio of husk and protein content was significant statistically. While increasing the ratio of husk and protein content, gamma ray application significantly reduced the weight of a thousand grains and test weight. In this research, plant height, spike length, number of grain per spike and weight of grain per spike were investigated on a single plant. Discriminant analysis was done and distance between groups was determined. The rate of plants similar to control in examined characters in M2 generation is 400 Gy 7.83%, 200 Gy 10%, 300 Gy 19.60% and 500 Gy 22.22%. When the coefficients containing linear separation functions are examined, it is determined that the most effective feature is spike length.


1967 ◽  
Vol 31 ◽  
pp. 469-471
Author(s):  
J. G. Duthie ◽  
M. P. Savedoff ◽  
R. Cobb
Keyword(s):  

A source of gamma rays has been found at right ascension 20h15m, declination +35°, with an uncertainty of 6° in each coordinate. Its flux is (1·5 ± 0·8) x 10-4photons cm-2sec-1at 100 MeV. Possible identifications are reviewed, but no conclusion is reached. The mechanism producing the radiation is also uncertain.


1994 ◽  
Vol 144 ◽  
pp. 635-639
Author(s):  
J. Baláž ◽  
A. V. Dmitriev ◽  
M. A. Kovalevskaya ◽  
K. Kudela ◽  
S. N. Kuznetsov ◽  
...  

AbstractThe experiment SONG (SOlar Neutron and Gamma rays) for the low altitude satellite CORONAS-I is described. The instrument is capable to provide gamma-ray line and continuum detection in the energy range 0.1 – 100 MeV as well as detection of neutrons with energies above 30 MeV. As a by-product, the electrons in the range 11 – 108 MeV will be measured too. The pulse shape discrimination technique (PSD) is used.


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