Screening for Phosphorus (P) Acquisition Efficient Genotypes and Identification of Sequence Variations among P-Efficient and Inefficient Genotypes in Chickpea (Cicer arietinum L.)

2020 ◽  
Author(s):  
Arpita Das ◽  
Loyavar Ramchander ◽  
Raghunath Sadhukhan ◽  
Joydeep Banerjee ◽  
Krishnendu Pramanik
Keyword(s):  
Cicer Arietinum ◽  
Yield Potential ◽  
P Availability ◽  
Nucleotide Polymorphisms ◽  
Marker Assisted Breeding ◽  
Single Nucleotide ◽  
Cicer Arietinum L ◽  
P Acquisition ◽  
Sequence Variations ◽  
Low Phosphorus

Low phosphorus (P) availability is the major abiotic constraint in chickpea (Cicer arietinum L.) cultivation across the globe as well as in India. Present study contemplated to identify high P-acquisition efficient chickpea genotypes suitable for P-deficient regions and sequence variation was detected among the tested P-acquisition efficient and inefficient genotypes. Initial screening was conducted with 104 diverse chickpea genotypes at two locations characterized by P-sufficient and deficient conditions. A panel of 14 chickpea genotypes having contrasting P-acquisition efficiency was extracted for further assessment under P-sufficient and deficient conditions. Along with the significant genetic variations, the P-efficient chickpea genotypes had high P-acquisition efficiency than P-inefficient genotypes in both the conditions. P-acquisition efficient genotypes recorded high biomass and good yield potential in P-deficient condition in comparison to P-inefficient genotypes. Sequence analysis of the CaSPX3 domain containing protein gene from P-acquisition efficient and inefficient genotypes identified two single nucleotide polymorphisms in the 5¢ - untranslated region. Present findings might be of great importance in future marker-assisted breeding in chickpea especially for P-deficient soil.

scholarly journals Inheritance Pattern and Molecular Markers for Resistance to Blackleg Disease in Cabbage

Plants ◽  
2019 ◽  
Vol 8 (12) ◽  
pp. 583
Author(s):  
Mostari Jahan Ferdous ◽  
Mohammad Rashed Hossain ◽  
Jong-In Park ◽  
Arif Hasan Khan Robin ◽  
Denison Michael Immanuel Jesse ◽  
...  
Keyword(s):  
Molecular Markers ◽  
High Resolution Melting ◽  
Leptosphaeria Maculans ◽  
Inbred Lines ◽  
Indel Marker ◽  
Detection Accuracy ◽  
Nucleotide Polymorphisms ◽  
Blackleg Disease ◽  
Marker Assisted Breeding ◽  
Single Nucleotide

The inheritance and causal loci for resistance to blackleg, a devastating disease of Brassicaceous crops, are yet to be known in cabbage (Brassica oleracea L.). Here, we report the pattern of inheritance and linked molecular marker for this trait. A segregating BC1 population consisting of 253 plants was raised from resistant and susceptible parents, L29 (♀) and L16 (♂), respectively. Cotyledon resistance bioassay of BC1 population, measured based on a scale of 0–9 at 12 days after inoculation with Leptosphaeria maculans isolate 03–02 s, revealed the segregation of resistance and ratio, indicative of dominant monogenic control of the trait. Investigation of potential polymorphism in the previously identified differentially expressed genes within the collinear region of ‘B. napus blackleg resistant loci Rlm1′ in B. oleracea identified two insertion/deletion (InDel) mutations in the intron and numerous single nucleotide polymorphisms (SNPs) throughout the LRR-RLK gene Bol040029, of which six SNPs in the first exon caused the loss of two LRR domains in the susceptible line. An InDel marker, BLR-C-InDel based on the InDel mutations, and a high resolution melting (HRM) marker, BLR-C-2808 based on the SNP C2808T in the second exon were developed, which predicated the resistance status of the BC1 population with 80.24%, and of 24 commercial inbred lines with 100% detection accuracy. This is the first report of inheritance and molecular markers linked with blackleg resistance in cabbage. This study will enhance our understanding of the trait, and will be helpful in marker assisted breeding aiming at developing resistant cabbage varieties.

scholarly journals Identification of Allelic Variation in Drought Responsive Dehydrin Gene Based on Sequence Similarity in Chickpea (Cicer arietinum L.)

2020 ◽  
Vol 11 ◽  
Author(s):  
Tapan Kumar ◽  
Neha Tiwari ◽  
Chellapilla Bharadwaj ◽  
Ashutosh Sarker ◽  
Sneha Priya Reddy Pappula ◽  
...  
Keyword(s):  
Single Nucleotide Polymorphisms ◽  
Drought Tolerance ◽  
Cicer Arietinum ◽  
Allelic Variation ◽  
Sequence Similarity ◽  
Nucleotide Polymorphisms ◽  
Residual Moisture ◽  
Single Nucleotide ◽  
The World ◽  
Dehydrin Gene

Chickpea (Cicer arietinum L.) is an economically important food legume grown in arid and semi-arid regions of the world. Chickpea is cultivated mainly in the rainfed, residual moisture, and restricted irrigation condition. The crop is always prone to drought stress which is resulting in flower drop, unfilled pods, and is a major yield reducer in many parts of the world. The present study elucidates the association between candidate gene and morpho-physiological traits for the screening of drought tolerance in chickpea. Abiotic stress-responsive gene Dehydrin (DHN) was identified in some of the chickpea genotypes based on the sequence similarity approach to play a major role in drought tolerance. Analysis of variance revealed a significant effect of drought on relative water content, membrane stability index, plant height, and yield traits. The genotypes Pusa1103, Pusa362, and ICC4958 were found most promising genotypes for drought tolerance as they maintained the higher value of osmotic regulations and yield characters. The results were further supported by a sequence similarity approach for the dehydrin gene when analyzed for the presence of single nucleotide polymorphisms (SNPs) and indels. Homozygous indels and single nucleotide polymorphisms were found after the sequencing in some of the selected genotypes.

Root exudation index: screening organic acid exudation and phosphorus acquisition efficiency in soybean genotypes

2016 ◽  
Vol 67 (10) ◽  
pp. 1096 ◽  
Author(s):  
Vengavasi Krishnapriya ◽  
Renu Pandey
Keyword(s):  
Molecular Weight ◽  
Root Exudation ◽  
Growth And Yield ◽  
Yield Potential ◽  
Secretory Proteins ◽  
P Availability ◽  
Screening Process ◽  
Soil P ◽  
P Acquisition ◽  
Soybean Genotypes

High-molecular-weight secretory proteins and low-molecular-weight exudates (carboxylates, phenols, free amino acids and sugars) released from roots of soybean (Glycine max (L.) Merr.) differentially influence genotypic phosphorus (P) acquisition efficiency (PAE). We hypothesised that genotypes with higher root exudation potential would exhibit enhanced P acquisition, and screened 116 diverse soybean genotypes by labelling shoots with 14CO2. A root exudation index (REI) derived from total 14C in the root exudate at sufficient (250 μm) and low (4 μm) P levels was used to classify genotypes for PAE. Genotypes with REI >2.25 exhibited significantly higher exudation at low than at sufficient P, which in turn increased PAE. Under low P availability, efficient genotypes exude a greater quantity of organic compounds into the rhizosphere. This increases P availability to meet the crop requirement, enabling the crop to produce consistent biomass and seed yield with reduced fertiliser addition. Such maintenance of growth and yield potential by mining the inherent soil P is a favourable trait in genotypes, reducing dependence on P fertilisers. Measuring REI at seedling stage to select P-efficient plants accelerates the screening process by accommodating large numbers of genotypes.

CDC Corinne desi chickpea

2009 ◽  
Vol 89 (3) ◽  
pp. 515-516 ◽  
Author(s):  
B. Taran ◽  
T. Warkentin ◽  
S. Banniza ◽  
A. Vandenberg
Keyword(s):  
Cicer Arietinum ◽  
Ascochyta Blight ◽  
Yield Potential ◽  
Ascochyta Rabiei ◽  
Western Canada ◽  
Canadian Prairies ◽  
Cicer Arietinum L ◽  
Crop Development ◽  
Cultivar Description ◽  
Release Program

CDC Corinne, a desi chickpea (Cicer arietinum L.) cultivar, was released in 2008 by the Crop Development Centre, University of Saskatchewan, for distribution to Select seed growers in western Canada through the Variety Release Program of the Saskatchewan Pulse Growers. CDC Corinne has a pinnate leaf type, fair resistance to ascochyta blight [Ascochyta rabiei (Pass.) Lab.], medium maturity, medium seed size and higher yield potential than Myles in the Brown and Dark Brown soil zones of the Canadian prairies. Key words: Chickpea, Cicer arietinum L., cultivar description, ascochyta blight

scholarly journals Complete overview of protein-inactivating sequence variations in 36 sequenced mouse inbred strains

2017 ◽  
Vol 114 (34) ◽  
pp. 9158-9163 ◽  
Author(s):  
Steven Timmermans ◽  
Marc Van Montagu ◽  
Claude Libert
Keyword(s):  
Mouse Genome ◽  
Inbred Strains ◽  
Nucleotide Polymorphisms ◽  
Genome Sequences ◽  
Single Nucleotide ◽  
Protein Coding ◽  
Stop Codons ◽  
Protein Coding Genes ◽  
Sequence Variations ◽  
Genetic Background Effects

Mouse inbred strains remain essential in science. We have analyzed the publicly available genome sequences of 36 popular inbred strains and provide lists for each strain of protein-coding genes that acquired sequence variations that cause premature STOP codons, loss of STOP codons and single nucleotide polymorphisms, and short in-frame insertions and deletions. Our data give an overview of predicted defective proteins, including predicted impact scores, of all these strains compared with the reference mouse genome of C57BL/6J. These data can also be retrieved via a searchable website (mousepost.be) and allow a global, better interpretation of genetic background effects and a source of naturally defective alleles in these 36 sequenced classical and high-priority mouse inbred strains.

CDC Vanguard desi chickpea

2009 ◽  
Vol 89 (3) ◽  
pp. 519-520
Author(s):  
T. Warkentin ◽  
B. Taran ◽  
S. Banniza ◽  
A. Vandenberg
Keyword(s):  
Cicer Arietinum ◽  
Ascochyta Blight ◽  
Yield Potential ◽  
Ascochyta Rabiei ◽  
High Yield ◽  
Cicer Arietinum L ◽  
Crop Development ◽  
Cultivar Description ◽  
Release Program ◽  
High Yield Potential

CDC Vanguard, a desi chickpea (Cicer arietinum L.) cultivar, was released in 2006 by the Crop Development Centre, University of Saskatchewan for distribution to Select seed growers in western Canada through the Variety Release Program of the Saskatchewan Pulse Growers. CDC Vanguard has a pinnate leaf type, fair resistance to ascochyta blight [Ascochyta rabiei (Pass.) Lab.], medium maturity, medium seed size and high yield potential in the Brown and Dark Brown soil zones of the Canadian prairies.Key words: Chickpea, Cicer arietinum L., cultivar description, ascochyta blight

scholarly journals Molecular genetics of thyroid cancer

2016 ◽  
Vol 98 ◽  
Author(s):  
MAHA REBAЇ ◽  
AHMED REBAЇ
Keyword(s):  
Thyroid Cancer ◽  
Prognostic Value ◽  
Genetic Factors ◽  
Nucleotide Polymorphisms ◽  
Genetic Determinants ◽  
Single Nucleotide ◽  
Sequence Variations ◽  
Disease Aetiology ◽  
Diagnostic And Prognostic Value

SummaryThe pathogenesis of the development and progression of thyroid cancer (TC) is far from being clear at present. Accumulated evidence suggests that it is a complex polygenic disorder for which genetic factors play an important role in disease aetiology. Here we review the literature to report the genetic variations and alterations that have been described in the aetiology of TC. The functional effects of some mutations and single nucleotide polymorphisms on TC are validated, establishing the role of sequence variations in this cancer. However, large prospective studies are still required to evaluate the diagnostic and prognostic value of these genetic determinants in clinical practice.

CDC Luna kabuli chickpea

2009 ◽  
Vol 89 (3) ◽  
pp. 517-518 ◽  
Author(s):  
B. Taran ◽  
T. Warkentin ◽  
R. Malhotra ◽  
S. Banniza ◽  
A. Vandenberg
Keyword(s):  
Cicer Arietinum ◽  
Ascochyta Blight ◽  
Yield Potential ◽  
Ascochyta Rabiei ◽  
Cicer Arietinum L ◽  
Crop Development ◽  
Cultivar Description ◽  
Late Maturity ◽  
Release Program ◽  
Similar Yield

CDC Luna, a kabuli chickpea (Cicer arietinum L.) cultivar, was released in 2007 by the Crop Development Centre, University of Saskatchewan, for distribution to Select seed growers in western Canada through the Variety Release Program of the Saskatchewan Pulse Growers. CDC Luna has a pinnate leaf type, fair resistance to ascochyta blight [Ascochyta rabiei (Pass.) Lab.], medium-late maturity, medium-large seed size and similar yield potential with the check cultivar Amit in the Brown and Dark Brown soil zones of the Canadian prairies.Key words: Chickpea, Cicer arietinum L., cultivar description, ascochyta blight

Modulation of ADAMTS13 secretion and specific activity by a combination of common amino acid polymorphisms and a missense mutation

Blood ◽  
2006 ◽  
Vol 107 (1) ◽  
pp. 118-125 ◽  
Author(s):  
Barbara Plaimauer ◽  
Jakob Fuhrmann ◽  
Gabriele Mohr ◽  
Waltraud Wernhart ◽  
Katharina Bruno ◽  
...  
Keyword(s):  
Amino Acid ◽  
Missense Mutation ◽  
Specific Activity ◽  
Phenotypic Expression ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Common Amino Acid ◽  
Sequence Variations ◽  
Adamts13 Deficiency ◽  
The Individual

Abstract Sequence analysis of the ADAMTS13 locus of 2 patients with hereditary thrombotic thrombocytopenic purpura (TTP) revealed the homozygous presence of 4 single nucleotide polymorphisms (SNPs) (R7W, Q448E, P618A, A732V) and a rare missense mutation (R1336W). Analysis of the individual effect of any amino acid exchanges showed that several sequence variations can interact with each other, thereby altering the phenotype of ADAMTS13 deficiency. Introduction of polymorphisms R7W, Q448E, and A732V had no or only minor effects on ADAMTS13 secretion. In contrast, P618A, R1336W, and the A732V-P618A combination strongly reduced ADAMTS13-specific activity and antigen levels. Surprisingly, R7W and Q448E were positive modifiers of ADAMTS13 secretion in the context of P618A and A732V but neither could rescue the severely reduced specific activity conferred by P618A. However, in the context of R1336W, polymorphisms R7W and Q448E enhanced the detrimental effect of the missense mutation and led to undetectable enzyme activity. We show that dependent on the sequence context, the same polymorphisms might be either positive or negative modifiers of gene expression. Our results might therefore be widely relevant to understanding the influence of polymorphisms on the phenotypic expression of complex diseases.

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