​Screening of Chickpea (Cicer arietinum L.) Genotypes for Germination and Early Seedling Growth under PEG 6000 Induced Drought Stress

2021 ◽  
Author(s):  
R. Himaja ◽  
K. Radhika ◽  
K. Bayyapu Reddy ◽  
M. Raghavendra
Keyword(s):  
Drought Stress ◽  
Seedling Growth ◽  
Root Length ◽  
Shoot Length ◽  
Growth Stages ◽  
Seedling Vigour ◽  
Peg 6000 ◽  
Early Seedling Growth ◽  
Vigour Index ◽  
Early Seedling

Background: Drought stress at germination and early growth stages hinders the seedling establishment in chickpea which ultimately affects the economic yield. Such adverse affects of drought can be mitigated by screening and identifying the tolerant genotypes of chickpea which is commonly cultivated under rain-fed conditions during post-rainy season. Methods: Effect of drought stress on germination and early seedling growth of thirty three chickpea genotypes was studied under four different concentrations of PEG 6000 (-0.3, -0.6, -0.9 and -1.2 MPa) along with control and hydration under laboratory conditions during 2018-19. Result: Significant variation was observed among the genotypes for germination, root length, shoot length and seedling vigour index under different concentrations of PEG 6000. Complete inhibition of germination was observed in most of the genotypes at -1.2 MPa. Based on the results obtained, JG 11 and NBeG 3 were considered as tolerant since they showed comparatively higher germination, root length, shoot length and seedling vigour even at -1.2 MPa, while NBeG 723 and NBeG 833 were considered as susceptible genotypes because of their poor germination and seedling growth even at lower levels of drought stress.

Physiological response of chickpea (Cicer arietinum L.) at early seedling stage under salt stress conditions

2019 ◽  
Author(s):  
Anita Mann ◽  
Gurpreet Kaur ◽  
Ashwani Kumar ◽  
Satish Kumar Sanwal ◽  
Jogendra Singh ◽  
...  
Keyword(s):  
Salt Stress ◽  
Salt Tolerance ◽  
Seedling Growth ◽  
Root Length ◽  
Dry Weight ◽  
Shoot Length ◽  
Seedling Stage ◽  
Tolerance Index ◽  
Early Seedling Growth ◽  
Early Seedling

Screening of chickpea lines for salt tolerance through seed germination and early seedling growth is crucial for their evaluation. Seeds of 30 chickpea genotypes were germinated on a sand bed irrigated with saline (3, 6, 9, 12 dS/m) and control solutions upto 30 days. At the early seedling stage (25-30 days), germination percentage, chlorophyll content, proline, root length, shoot length and seedling dry weight were found to be affected due to salinity. Salt tolerance index (STI) for plant biomass maintained a significant correlation with chlorophyll, proline, shoot length, and root length, which indicated that these parameters could be used as selection criteria for screening chickpea genotypes against salt stress. Significant differences in shoot length, root length, and seedling dry weight in 30-day-old seedlings were observed among selected chickpea genotypes as well. From the overall observation of germination characterstics and early seedling growth, it is concluded that the chickpea genotypes, HC-1, HC-5, ICC 867, ICC 5003, H-10-41 showed better salt tolerance as compared to the available salt tolerant check variety.

Impact of Seed Maturation and Drought on Seed Germination and Early Seedling Growth in White Clover (Trifolium repens L.)

2021 ◽  
Author(s):  
Ruqiang Tong ◽  
Xinyuan Liu ◽  
Bifan Mu ◽  
Junfeng Wang ◽  
Mengxing Liu ◽  
...  
Keyword(s):  
Seedling Growth ◽  
White Clover ◽  
Germination Percentage ◽  
Seed Maturation ◽  
Percentage Germination ◽  
Seedling Vigour ◽  
Early Seedling Growth ◽  
Vigour Index ◽  
Early Seedling ◽  
The Mean

Background: Understanding the drought tolerance of white clover seeds during germination and early seedling growth and how that is linked to seed maturation in legume species, is crucial to developing new stand establishments.Methods: An experiment was conducted in Randomized Block design in two factorial concept to explore how seed maturation and drought tolerance influence seed germination and early seedling growth using the two factors: seed coat color (yellow and brown, two levels as one factor) and drought intensity (control: 0 MPa [distilled water], mild drought: -0.2 MPa, moderate drought: -0.4 MPa and severe drought: -0.6 MPa, four levels as another factor).Result: In contrast to the yellow-coated seeds, the mean germination percentage, germination index, seedling vigour index and radicle length of the brown-coated seeds were lowered by 7.9%, 23.1%, 20.5% and 12.9%, respectively. Compared to the control, introducing drought conditions reduced the mean of germination percentage, germination index, seedling vigour index and radicle length of yellow-coated seeds by 52.4%, 64.1%, 41.9% and 23.2%, respectively. Meanwhile, drought affected brown-coated seeds more than yellow-coated seeds. The results indicating that the seed quality of white clover seed lots with yellow-colored coats higher than the seed lots with brown-coloured coats. In agronomic practice, a larger proportion of yellow-coated seeds should be harvested to improve new stands successful establishment under environment easy to dry.

scholarly journals Response of Bioinoculants to Early Seedling Growth in Sunflower (Helianthus annuus, L.)

2018 ◽  
Vol 1 (2) ◽  
pp. 48-54
Author(s):  
Shyam Sundar Lakshman ◽  
M.K. Ghodke
Keyword(s):  
Seed Germination ◽  
Seedling Growth ◽  
Growth Regulators ◽  
Shoot Length ◽  
Growth Stages ◽  
Early Seedling Growth ◽  
Root And Shoot Length ◽  
Early Seedling ◽  
Secondary Roots ◽  
Better Than

The present study was carried out to evaluate the response of different bioagents/biofungicides and growth regulators on seed germination and early seedling growth of sunflower. Eleven (11) treatments were established for the present investigation. Higher germination was observed in T5, T6/T7, T10 and T11 treatments in contrast to other treatments. Treatments T7, T3 and T11 were found significantly better than all other treatments. Generally, root and shoot length increased with the advancement of growth stages. T4 showed higher number of secondary roots in comparison to all other treatments. The shoot length and root length in all the cases (irrespective of the cultivars: DRSH-1, DRSF-108, LSFH-171) were highly influenced by the bioinoculants and chemicals; however, influence of bioagents was found better than the chemicals. Similarly, the seedling weight in 96 hours after sowing and 144 hours after sowing in all the cultivars was reported higher than when the seeds were treated with bioinoculants, which reflected the efficacy of the bioinoculants compared to others.

scholarly journals First Report of Sesame Mutants Tolerant to Severe Drought Stress during Germination and Early Seedling Growth Stages

Plants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1166
Author(s):  
Mohamed Kouighat ◽  
Hafida Hanine ◽  
Mohamed El Fechtali ◽  
Abdelghani Nabloussi
Keyword(s):  
Drought Stress ◽  
Seedling Growth ◽  
Germination Rate ◽  
Germination Percentage ◽  
Growth Stages ◽  
Severe Drought ◽  
First Report ◽  
Early Seedling Growth ◽  
Early Seedling ◽  
Sesame Genotypes

In the context of climate change and water scarcity, there is a need to develop and use drought-tolerant sesame cultivars. This study was conducted to evaluate the response of 13 sesame genotypes, including 11 mutants and their wild-types, to drought during germination and early seedling growth. Moderate and severe drought stress was simulated by applying polyethylene glycol (PEG) at two osmotic potentials, −0.6 MPa and −1.2 MPa, respectively, on seeds of two successive mutant generations, M2 and M3. The parameters measured or calculated were germination percentage (GP), germination rate (GR), mean germination time (MGT), root length (RL), shoot length (SL), root to shoot ratio (RSR), and the seedling vigor index (SVI). Results showed the significant effect of genotype, drought, and drought × genotype interaction on all parameters investigated. Under severe drought, seeds of seven genotypes, including wild types, were not able to germinate. There was a drastic decline of all parameters for the rest, except MGT and RSR, which markedly increased. Interestingly, two mutants, “ML2-5” and “ML2-10”, were identified as the most tolerant to severe drought and the most stable over both generations. The present work is the first report of sesame germplasm with such a high level of tolerance to drought during germination and early seedling growth stages.

scholarly journals Development of priming technology for enhanced planting value of seeds in kabuli chickpea (Cicer arietinum L.)

2021 ◽  
Vol 13 (2) ◽  
pp. 735-743
Author(s):  
Umesh Hiremath ◽  
Basave Gowda ◽  
Lokesh G.Y . ◽  
B. S. Ganiger
Keyword(s):  
Root Length ◽  
Seed Quality ◽  
Dry Weight ◽  
Germination Percentage ◽  
Quality Parameters ◽  
Shoot Length ◽  
Growth And Yield ◽  
Seedling Vigour ◽  
Seedling Length ◽  
Vigour Index

The continuous rise in global population demands more food production under limited land resources accompanied by climate change. More oversupply of quality seeds to meet ever-growing food demand is a highly challenging task. Seeds with low vigour level limit the growth and yield of crops at varied field conditions. Hence, an experiment was conducted to develop a technique for the fast and homogeneous growth of kabuli chickpea seeds. The priming treatments evaluated in the present study enhanced the various seed quality parameters of kabuli chickpea significantly when compared to control. Hydro-priming for 4 hours (at 20°C) and air drying for 72 hours showed better results compared to rest of the treatments both in fresh seeds which recorded higher first count (66.38%), germination (92.50%) root length (11.85 cm), shoot length (6.16), mean seedling length (18.01), dry seedling weight (747 mg), seedling vigour index-I (1665), higher speed of emergence (0.3992) and old seeds with higher first count (61.88%), germination (74.13%) root length (8.15 cm), shoot length (5.85 cm), mean seedling length (14.00 cm), seedling dry weight (611 mg), seedling vigour index-I (1056), higher speed of emergence (0.3814). The aged seeds have recorded 29 % increase in germination percentage in contrast to 8 percent increases in fresh seeds. Kabuli chickpea seeds could be subjected to hydropriming, an affordable, alternative, and eco-friendly technique for improving seed and seedling vigour of kabuli chickpea.

scholarly journals Fungicidal seed treatment on germination and seedling vigour of lentil var. BINA Masur-3

2017 ◽  
Vol 3 (1) ◽  
pp. 140-144
Author(s):  
A Rokib ◽  
MS Monjil
Keyword(s):  
Seed Germination ◽  
Root Length ◽  
Seed Treatment ◽  
Shoot Length ◽  
Seedling Vigour ◽  
Green House ◽  
Seed Pathology ◽  
Vigour Index ◽  
Positive Effect ◽  
Improve Seed Germination

Effectiveness of six fungicides viz., Provax-200 (Carboxin+Thiram), Bavistin DF (Carbendazim), Dithane M-45 (Mancozeb), Secure (Mancozeb+Fenamidone), Antracol (propineb) and Daconil (Chlorothalonil) were evaluated to improve seed germination and seedling vigour of lentil variety BINA Masur-3. The experiment was conducted in the Green House of Seed Pathology Centre, Bangladesh Agricultural University, Mymensingh. Positive effect of seed treatment with fungicides on seed germination and seedling vigour of lentil was recorded. At 20 DAS, increased seed germination over control was observed in treated seeds with Deconil (10.40%) and Antracol (10.00%) followed by Dithane M-45 (7.20%). Seeds treated with Deconil and Dithane M-45 produced seedlings with higher shoot length, root length and seedling vigour. At 20 DAS, higher percent increased vigour index over control was found in Dithane M-45 (24.64%) and Deconil (22.44%), respectively.Asian J. Med. Biol. Res. March 2017, 3(1): 140-144

Seed coating reduces respiration losses and affects sugar metabolism during germination and early seedling growth in cereals

2015 ◽  
Vol 42 (2) ◽  
pp. 209 ◽  
Author(s):  
Linda Gorim ◽  
Folkard Asch
Keyword(s):  
Seedling Growth ◽  
Sucrose Synthase ◽  
Sugar Metabolism ◽  
Seedling Vigour ◽  
Early Seedling Growth ◽  
Plant Life ◽  
Early Seedling ◽  
Successful Establishment ◽  
The Relationship ◽  
Seed Coats

Seed germination and the successful establishment of young seedlings is an important aspect of plant life. Seed coats are used to improve stand establishment and early seedling vigour. Seedlings growing from hydro-absorber coated barley, rye and wheat with coat-shares greater than 75% of the average seed have been shown to promote better seedling growth compared with those seedlings growing from uncoated seeds. We investigated how and why these seedlings performed better by analysing the proportion of grain reserves mobilised for growth and respiration as well as how both sucrose and glucose available in the embryo translated into seedling growth in the presence or absence of seed coats containing hydro-absorber gel. We found that mobilisation efficiency was higher, resulting in higher biomass in these cereals when they were coated. The relationship between sucrose and glucose available to the seedling as well as its correlation with early seedling growth indicate a switch in the enzymatic cleavage of embryonic sucrose from invertase to sucrose synthase. This in turn indicates that in coated seeds, embryonic tissue must be hypoxic leading to a more efficient use of glucose and thus reduced respiration losses during germination.

scholarly journals Seed quality enhancement through biopriming in common bean (Phaseolus vulgaris. L)

2017 ◽  
Vol 9 (3) ◽  
pp. 1740-1743
Author(s):  
S. P. Monalisa ◽  
J. K. Beura ◽  
R. K. Tarai ◽  
M. Naik
Keyword(s):  
Phaseolus Vulgaris ◽  
Trichoderma Harzianum ◽  
Root Length ◽  
Seed Quality ◽  
Dry Weight ◽  
Shoot Length ◽  
Quality Enhancement ◽  
Phaseolus Vulgaris L ◽  
Seedling Vigour ◽  
Vigour Index

An experiment on seed quality enhancement of common bean (Phaseolus vulgaris L.) var. S 9 (local) was conducted at the department of seed science and technology, OUAT, Bhubaneswar during 2013-14 by use of three biocontrol agents viz. Trichoderma viride, Trichoderma harzianum, Pseudomonas fluorescence. Seeds were bi-oprimed with the biocontrol agents at 40, 50 and 60 % concentration for 4,8,12 and 16 hours of soaking. Seeds were also hydro primed for 4,8,12 and 16 hours. Unprimed dry seed resulted in germination (69 %), shoot length (27.5 cm), root length (14 cm), seedling dry weight (1.71g), SVI-I (2859.2), SVI-II (118.0) and speed of germination (5.8) while hydro primed seeds resulted in germination (72%), shoot length (31.9 cm), root length (15 cm), seedling dry weight (1.80 g), SVI-1 (3375.9) SVI-II (129.8) and speed of germination (6.7). Trichoderma harzianum at 40% con-centration and for 4 hours of soaking resulted enhancement of above quality parameter like 13.0 % in germination, 21.1 % in shoot length, 20.7 % in root length, 31.6 % in seedling dry weight, 36 % in seedling vigour index-I, 48.1 % in seedling vigour index-II and 58.6 % in speed of germination over unprimed seeds. Bio priming with P. fluorescence ( at 40% concentration and for 4 hour) closely followed and at par with best treatment with 11.6 %, 18.2 %, 16.4 %, 30.4 %, 30.7 % and 56.9 % enhancement of above mentioned quality parameters, respectively.

Inhibitory Effect of Aqueous Extracts of Tree-legumes on Germination and Seedling Growth of Food Legume, Green Gram (Vigna radiata L.)

2021 ◽  
Author(s):  
Anbarasu Mariyappillai ◽  
Swaminathan Chitraputhira Pillai
Keyword(s):  
Root Length ◽  
Vigna Radiata ◽  
Shoot Length ◽  
Green Gram ◽  
Allelopathic Effect ◽  
Aqueous Extracts ◽  
Seedling Vigour ◽  
Delonix Regia ◽  
Plant Parts ◽  
Vigour Index

Background: Green gram (Vigna radiata), the third most important pulse crop, is grown in tank-fed, command areas after the harvest of paddy. In such areas, legume trees like Delonix regia and Peltophorum pterocarpum are commonly found and tree parts often fell in watercourses and were flown to crop fields along with irrigation water. Hence, it is necessary to study whether any allelopathic effect is present in these parts of trees which may affect the growth and development of green gram grown in farmland crops. Methods: Collected different tree parts viz., bark, leaf and fresh flower, a matured pod of Delonix regia and Peltophorum pterocarpum, cleaned and soaked distilled water at weight/volume ratio of 1:10 for 24 hrs. Aqueous extracts were filtered and tested for inhibition of green gram. Pot culture experiments were conducted with 10 treatments and four replications. Germination (%), shoot length (cm), root length (cm), fresh weight (mg), dry weight (mg), seedling vigour index-I and vigour index-II in green gram were recorded and the magnitude of inhibition versus simulation in bioassay was compared through response index.Result: Among the plant parts, Delonix regia flower and Peltophorum pterocarpum matured pods suppressed germination (45.00 and 60.00%), shoot length (13.87 and 13.39 cm), root length (4.27 and 10.49 cm), seedling vigour index-I (624.15 and 803.40) and seedling vigour index-II (960.75 and 948.00) respectively in green gram. This might due to presence of inhibitory chemicals in flowers of Delonix regia and matured pods of Peltophorum pterocarpum compared to other plant parts of trees.

Comparative toxic effects of some xenobiotics on the germination and early seedling growth of jack pine (Pinusbanksiana Lamb.) and white birch (Betulapapyrifera Marsh.)

1981 ◽  
Vol 11 (4) ◽  
pp. 796-804 ◽  
Author(s):  
Pearl Weinberger ◽  
Ritta Vladut
Keyword(s):  
Seedling Growth ◽  
Root Length ◽  
Jack Pine ◽  
Atp Content ◽  
White Birch ◽  
Early Seedling Growth ◽  
Sprout Length ◽  
Early Seedling ◽  
Sensitive Parameters ◽  
All Treatment

Seeds of jack pine (Pinusbanksiana Lamb.) and white birch (Betulapapyrifera March.) were germinated in homogeneous emulsions or aqueous tank-mix solutions of fenitrothion or Matacil and their respective adjuvants: Atlox and Aerotex, or diluent oil No. 585 and nonylphenol. Percentage and peak germination values, water uptake, sprout length, ATP content, and morphological modifications were recorded from 0 to 14 or 21 days. Apart from 100 ppm fenitrothion which stimulated germination values, germination in jack pine was only marginally affected by any of the treatments; in contrast, white birch was negatively affected by all treatments. The most sensitive parameters of toxicity were the sprout length and ATP content after 14 days growth. Aberrant hypocotyl/root length ratios were evidenced in pine seeds after exposure to xenobiotic treatments which did not affect the germinative capacity of seeds. ATP content in the 14-day-old pine and birch seedlings was consistently higher than controls in all treatment sets.

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