Evaluation of sero-prevalence of toxoplasma gondii infection insheep using different immunodiagnostic methods

2016 ◽  
Author(s):  
Sadaf Niaz ◽  
Rafi Ullah ◽  
Bahrawar Said ◽  
Sumaira Shams ◽  
Saba Shahzadi ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Prevalence Rate ◽  
Latex Agglutination ◽  
Agglutination Test ◽  
Infected Sheep ◽  
Lateral Flow ◽  
Latex Agglutination Test ◽  
Toxoplasma Gondii Infection ◽  
Female Sheep ◽  
Male Sheep

The main aim of the present study was to investigate the seroprevalence of Toxoplasma gondii infection in sheep of seven different regions of District Dir (Lower), Pakistan. Lateral Flow Chromatographic Immunoassay (LFCI), Latex Agglutination Test (LAT) and Enzyme-Linked immunosorbentAssay (ELISA) were used for the detection of T.gondii infection in sheep.Out of 175 sheepsera samples analyzed, 72 (41.14%) were found positive for T. gondii antibodies. Overall prevalence in sheep indicated highest infection at Khall (48%), followed by Samarbagh (44%), LalQilla (44%), Adenzai (44%),Balambat (40%), Timergara (36%), and lowest in Munda (32%). The occurrence of T. gondii infection in older sheep was higher (48.42%) than younger ones (32.5%), indicating older sheep to be more susceptible to toxoplasmosis as compared to younger ones. The prevalence rate in female sheep was higher (42.4%) as compared to male sheep (38%). This study demonstrates that older female sheep have low immunity to toxoplasmosis. The present study showed that infected sheep might be a probable risk for human toxoplasmosis. Therefore, proper measures should be taken to control and avoid toxoplasmosis in sheep in this region.

Comparison of LFCI, LAT and ELISA for the diagnosis of toxoplasmosis in goats

2015 ◽  
Author(s):  
Sadaf Niaz ◽  
Rafi Ullah ◽  
Bahrawar Said ◽  
Sumaira Shams ◽  
Naser M. AbdEl-Salam ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Immunosorbent Assay ◽  
Latex Agglutination ◽  
Agglutination Test ◽  
Lateral Flow ◽  
Latex Agglutination Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Blood Samples ◽  
Male And Female ◽  
Toxoplasma Gondii Infection

This study was carried out in order to investigate the seroprevalence of Toxoplasma gondii infection in goats of seven different regions of District Dir (Lower), Pakistan. Lateral Flow Chromatographic Immunoassay (LFCI), Latex Agglutination Test (LAT) and Enzyme-Linked Immunosorbent Assay (ELISA) were used for the detection of T. gondii infection in goats. A total of 175 blood samples were collected from goats. Out of which 70 (40%) were found positive for T. gondii. The prevalence of toxoplasmosis among goats in seven areas of District Dir (Lower) was found high at Samarbagh (48%), Balambat (44%) followed by khall (40%), Munda (40%), LalQilla (36%) and lowest in Adenzai and Timergara (36%). This study demonstrated that the prevalence in older goats was significantly (P< 0.05) higher (48.91%) than younger ones (30.12%). The seroprevalence in male and female goats were 16 (29%) and 54 (45%) respectively.

scholarly journals Seroepidemiologi toksoplasmosis pada masyarakat di Desa Kumu Kabupaten Minahasa tahun 2015

2016 ◽  
Vol 4 (1) ◽  
Author(s):  
Vanessa J. T. Seran ◽  
Billy J. Kepel ◽  
Fatimawali .
Keyword(s):  
Toxoplasma Gondii ◽  
Latex Agglutination ◽  
Agglutination Test ◽  
Latex Agglutination Test ◽  
Serological Tests ◽  
Cross Sectional ◽  
History Of ◽  
Blood Specimens ◽  
Toxoplasma Gondii Infection ◽  
Specific Symptoms

Abstract: Toxoplasmosis is a disease caused by Toxoplasma gondii in humans and also in animals. Toxoplasma gondii infection is widespread in the world, about 20-90% of the populations have been exposed to this parasite, and most take place without showing specific symptoms. The purpose of this study was to determine the seroepidemiology of toxoplasmosis among the villagers of kumu in district minahasa on 2015. This study was a cross sectional descriptive study. The study population was Kumu Village community. This study using blood specimens to be tested by latex agglutination test and interviews to determine the distribution of toxoplasmosis is based on risk factors. Seropositive obtained if agglutination occurs on the results of serological tests. Respondents consisted of 20 (90.90%) females and 2 (9.10%) men. The results showed 11 (50%) of respondents has toxoplasma seropositivity were distributed by age, sex, level of education, occupation, diet, hygiene, exposure to dogs, exposure to cats, and a history of miscarriage.Keywords: latex agglutination test, parasites, seropositivity, toxoplasma gondiiAbstrak: Toksoplasmosis merupakan penyakit yang disebabkan oleh Toxoplasma gondii pada manusia dan juga pada hewan. Infeksi Toxoplasma gondii tersebar luas di dunia, sekitar 20-90% populasi dunia pernah terpapar parasit ini, dan sebagian besar berlangsung tanpa menunjukkan gejala yang spesifik. Tujuan penelitian ini adalah untuk mengetahui seroepidemiologi toksoplasmosis pada masyarakat di Desa Kumu Kabupaten Minahasa tahun 2015. Penelitian ini merupakan penelitian deskriptif cross sectional. Populasi penelitian ini adalah masyarakat Desa Kumu. Penelitian ini menggunakan spesimen darah untuk diuji dengan uji aglutinasi lateks dan hasil wawancara untuk mengetahui distribusi toksoplasmosis berdasarkan faktor resiko. Seropositif didapat bila terjadi aglutinasi pada hasil tes serologis. Responden terdiri dari 20 (90,90%) wanita dan 2 (9,10%) pria. Hasil penelitian menunjukkan 11 (50%) responden seropositif toksoplasmosis yang terdistribusi berdasarkan umur, jenis kelamin, tingkat pedidikan, pekerjaan, pola makan, higienitas, paparan anjing, paparan kucing, dan riwayat keguguran.Kata kunci: uji aglutinasi lateks, parasit, seropositif, toxoplasma gondii

Seroepidemiology of Toxoplasma gondii in pet dogs and cats in Beijing, China

2008 ◽  
Vol 53 (3) ◽  
Author(s):  
Jinhai Yu ◽  
Jun Ding ◽  
Zhaofei Xia ◽  
Degui Lin ◽  
Yili Li ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Immunosorbent Assay ◽  
Age Groups ◽  
Latex Agglutination ◽  
Agglutination Test ◽  
Latex Agglutination Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Pet Dogs ◽  
Significant Difference ◽  
Beijing China

AbstractSera from 534 pet dogs and 335 pet cats from Beijing (China) were tested for anti-Toxoplasma gondii antibodies using an enzyme-linked immunosorbent assay or the latex agglutination test. The seropositivity by year, season, sex and age was analysed. Overall, 128 dogs (24.0%) and 50 cats (14.9%) had antibodies to T. gondii. When analysed by season, the highest seroprevalence was found in spring for dogs (31.3%) and cats (25.1%), and the differences in seroprevalence by season was statistically significant in cats (P<0.01) but not in dogs. The seroprevalence in male dogs (23.7%) and cats (15.1%) were slightly higher than their female counterparts (18.0% in dogs and 12.3% in cats). There was no obvious pattern of seropositivity or significant difference in different age groups in dogs or cats; nonetheless, a high proportion of dogs at 4 years of age were positive to T. gondii (31.8%) while cats with relatively high seropositivity rates were at 1 or 3.4 years of age (13.14%).

scholarly journals Latex agglutination test based prevalence of Toxoplasma gondii in native Korean cattle

2011 ◽  
Vol 51 (1) ◽  
pp. 59-61
Author(s):  
Eun-Sik Song ◽  
Sang-Il Jung ◽  
Bae-Keun Park ◽  
Myung-Jo You ◽  
Duck-Hwan Kim ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Latex Agglutination ◽  
Agglutination Test ◽  
Latex Agglutination Test ◽  
Korean Cattle
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