Comparison of LFCI, LAT and ELISA for the diagnosis of toxoplasmosis in goats

2015 ◽  
Author(s):  
Sadaf Niaz ◽  
Rafi Ullah ◽  
Bahrawar Said ◽  
Sumaira Shams ◽  
Naser M. AbdEl-Salam ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Immunosorbent Assay ◽  
Latex Agglutination ◽  
Agglutination Test ◽  
Lateral Flow ◽  
Latex Agglutination Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Blood Samples ◽  
Male And Female ◽  
Toxoplasma Gondii Infection

This study was carried out in order to investigate the seroprevalence of Toxoplasma gondii infection in goats of seven different regions of District Dir (Lower), Pakistan. Lateral Flow Chromatographic Immunoassay (LFCI), Latex Agglutination Test (LAT) and Enzyme-Linked Immunosorbent Assay (ELISA) were used for the detection of T. gondii infection in goats. A total of 175 blood samples were collected from goats. Out of which 70 (40%) were found positive for T. gondii. The prevalence of toxoplasmosis among goats in seven areas of District Dir (Lower) was found high at Samarbagh (48%), Balambat (44%) followed by khall (40%), Munda (40%), LalQilla (36%) and lowest in Adenzai and Timergara (36%). This study demonstrated that the prevalence in older goats was significantly (P< 0.05) higher (48.91%) than younger ones (30.12%). The seroprevalence in male and female goats were 16 (29%) and 54 (45%) respectively.

Seroepidemiology of Toxoplasma gondii in pet dogs and cats in Beijing, China

2008 ◽  
Vol 53 (3) ◽  
Author(s):  
Jinhai Yu ◽  
Jun Ding ◽  
Zhaofei Xia ◽  
Degui Lin ◽  
Yili Li ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Immunosorbent Assay ◽  
Age Groups ◽  
Latex Agglutination ◽  
Agglutination Test ◽  
Latex Agglutination Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Pet Dogs ◽  
Significant Difference ◽  
Beijing China

AbstractSera from 534 pet dogs and 335 pet cats from Beijing (China) were tested for anti-Toxoplasma gondii antibodies using an enzyme-linked immunosorbent assay or the latex agglutination test. The seropositivity by year, season, sex and age was analysed. Overall, 128 dogs (24.0%) and 50 cats (14.9%) had antibodies to T. gondii. When analysed by season, the highest seroprevalence was found in spring for dogs (31.3%) and cats (25.1%), and the differences in seroprevalence by season was statistically significant in cats (P<0.01) but not in dogs. The seroprevalence in male dogs (23.7%) and cats (15.1%) were slightly higher than their female counterparts (18.0% in dogs and 12.3% in cats). There was no obvious pattern of seropositivity or significant difference in different age groups in dogs or cats; nonetheless, a high proportion of dogs at 4 years of age were positive to T. gondii (31.8%) while cats with relatively high seropositivity rates were at 1 or 3.4 years of age (13.14%).

scholarly journals Serological diagnosis of Toxoplasmosis by specific antibody in camels of Wasit province, Iraq

2018 ◽  
Vol 1 (29) ◽  
pp. 412-423
Author(s):  
Basim Mohammed Hanon
Keyword(s):  
Public Health ◽  
Toxoplasma Gondii ◽  
Immunosorbent Assay ◽  
Specific Antibody ◽  
Latex Agglutination ◽  
Agglutination Test ◽  
Serological Diagnosis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Blood Samples ◽  
Zoonotic Parasite

Background: toxoplasma gondii is a zoonotic parasite, more added a major public health is worldwide because have high distribution in livestock. Objectives: the main aim of this study determine the occurrence of the seroepidemiological toxoplasmosis in camels in waist province of Iraq from November 2016 to April 2017. Materials and Methods: blood samples collected of animals randomly were included six different groups of animals were diagnosed by A Latex agglutination test (LAT) and indirect enzyme linked immunosorbent assay (ELISA) kits.

Evaluation of sero-prevalence of toxoplasma gondii infection insheep using different immunodiagnostic methods

2016 ◽  
Author(s):  
Sadaf Niaz ◽  
Rafi Ullah ◽  
Bahrawar Said ◽  
Sumaira Shams ◽  
Saba Shahzadi ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Prevalence Rate ◽  
Latex Agglutination ◽  
Agglutination Test ◽  
Infected Sheep ◽  
Lateral Flow ◽  
Latex Agglutination Test ◽  
Toxoplasma Gondii Infection ◽  
Female Sheep ◽  
Male Sheep

The main aim of the present study was to investigate the seroprevalence of Toxoplasma gondii infection in sheep of seven different regions of District Dir (Lower), Pakistan. Lateral Flow Chromatographic Immunoassay (LFCI), Latex Agglutination Test (LAT) and Enzyme-Linked immunosorbentAssay (ELISA) were used for the detection of T.gondii infection in sheep.Out of 175 sheepsera samples analyzed, 72 (41.14%) were found positive for T. gondii antibodies. Overall prevalence in sheep indicated highest infection at Khall (48%), followed by Samarbagh (44%), LalQilla (44%), Adenzai (44%),Balambat (40%), Timergara (36%), and lowest in Munda (32%). The occurrence of T. gondii infection in older sheep was higher (48.42%) than younger ones (32.5%), indicating older sheep to be more susceptible to toxoplasmosis as compared to younger ones. The prevalence rate in female sheep was higher (42.4%) as compared to male sheep (38%). This study demonstrates that older female sheep have low immunity to toxoplasmosis. The present study showed that infected sheep might be a probable risk for human toxoplasmosis. Therefore, proper measures should be taken to control and avoid toxoplasmosis in sheep in this region.

scholarly journals Seroepidemiologi toksoplasmosis pada masyarakat di Desa Kumu Kabupaten Minahasa tahun 2015

2016 ◽  
Vol 4 (1) ◽  
Author(s):  
Vanessa J. T. Seran ◽  
Billy J. Kepel ◽  
Fatimawali .
Keyword(s):  
Toxoplasma Gondii ◽  
Latex Agglutination ◽  
Agglutination Test ◽  
Latex Agglutination Test ◽  
Serological Tests ◽  
Cross Sectional ◽  
History Of ◽  
Blood Specimens ◽  
Toxoplasma Gondii Infection ◽  
Specific Symptoms

Abstract: Toxoplasmosis is a disease caused by Toxoplasma gondii in humans and also in animals. Toxoplasma gondii infection is widespread in the world, about 20-90% of the populations have been exposed to this parasite, and most take place without showing specific symptoms. The purpose of this study was to determine the seroepidemiology of toxoplasmosis among the villagers of kumu in district minahasa on 2015. This study was a cross sectional descriptive study. The study population was Kumu Village community. This study using blood specimens to be tested by latex agglutination test and interviews to determine the distribution of toxoplasmosis is based on risk factors. Seropositive obtained if agglutination occurs on the results of serological tests. Respondents consisted of 20 (90.90%) females and 2 (9.10%) men. The results showed 11 (50%) of respondents has toxoplasma seropositivity were distributed by age, sex, level of education, occupation, diet, hygiene, exposure to dogs, exposure to cats, and a history of miscarriage.Keywords: latex agglutination test, parasites, seropositivity, toxoplasma gondiiAbstrak: Toksoplasmosis merupakan penyakit yang disebabkan oleh Toxoplasma gondii pada manusia dan juga pada hewan. Infeksi Toxoplasma gondii tersebar luas di dunia, sekitar 20-90% populasi dunia pernah terpapar parasit ini, dan sebagian besar berlangsung tanpa menunjukkan gejala yang spesifik. Tujuan penelitian ini adalah untuk mengetahui seroepidemiologi toksoplasmosis pada masyarakat di Desa Kumu Kabupaten Minahasa tahun 2015. Penelitian ini merupakan penelitian deskriptif cross sectional. Populasi penelitian ini adalah masyarakat Desa Kumu. Penelitian ini menggunakan spesimen darah untuk diuji dengan uji aglutinasi lateks dan hasil wawancara untuk mengetahui distribusi toksoplasmosis berdasarkan faktor resiko. Seropositif didapat bila terjadi aglutinasi pada hasil tes serologis. Responden terdiri dari 20 (90,90%) wanita dan 2 (9,10%) pria. Hasil penelitian menunjukkan 11 (50%) responden seropositif toksoplasmosis yang terdistribusi berdasarkan umur, jenis kelamin, tingkat pedidikan, pekerjaan, pola makan, higienitas, paparan anjing, paparan kucing, dan riwayat keguguran.Kata kunci: uji aglutinasi lateks, parasit, seropositif, toxoplasma gondii

scholarly journals An investigation of sheep toxoplasmosis in Basrah Province / Iraq

2010 ◽  
Vol 9 (3) ◽  
pp. 128
Author(s):  
K. M. Al-Saad ◽  
Saad Hashim Al-Husseiny
Keyword(s):  
Toxoplasma Gondii ◽  
Immunosorbent Assay ◽  
Indirect Elisa ◽  
Elisa Test ◽  
Latex Agglutination ◽  
Agglutination Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Study Results ◽  
Significant Difference ◽  
The Difference

The objective of this study was to investigate Toxoplasma gondii antibodies among sheep in different regions of Basrah province (including Al-Mdayna, Shatt Al-Arab, Al-Basrah, Al-Zubayr, and Abu Al-Khasib). The study was started in Oct. 2008 and was finished in May 2009, using latex agglutination test (LAT) and indirect enzyme linked immunosorbent assay (ELISA) IgG, 309 adult sheep were randomly selected from 15 herds among different ages and both sexes and used in this study, including 62 pregnant ewes, 185 non-pregnant ewes, 14 aborted ewes, and 48 rams. Results showed, that 60.84% were seropositive by LAT, whereas 51.11% were seropositive by ELISA IgG test, among animals used in this study, results detected that 79.03% pregnant ewes (highest value), 56.75% nonpregnant ewes,71.40% aborted ewes and 50% Rams (lowest value) were seropositive by LAT, whereas 56.52% pregnant ewes, 51.11% non-pregnant ewes, 83.33% aborted ewes (highest value), and 31.25% Rams (lowest value) were seropositive by indirect ELISA IgG. Moreover, among regions of Basrah province, the details of percentage of T.gondii antibodies were 54.54% in AL-Basrah , 71.43% in Abu Al-Khasib (highest value), 57.35% in Al- Mdayna, 47.83% in Shatt Al-Arab (lowest value), and 67.16% in Al-Zubayr by LAT, whereas 63.64% in AL-Basrah (highest value), 22.73% in Abu Al-Khasib (lowest value), 57.89% in Al-Mdayna, 50% in Shatt Al-Arab and 61.90% in Al-Zubayr by indirect ELISA test. Although the difference observed in the percentage of Toxoplasma gondii antibodies among different regions of Basrah, there was no significant difference P>0.05 detected LAT, whereas in the indirect ELISA IgG test there was significant difference P<0.05. Ewes showed high percentages 62.83%, 55.40% of toxoplasmosis than rams 50 %, 31.25% by LAT and ELISA test respectively. The highest titer was 1/4 28.57% were detected in pregnant ewes and lowest titers were 1/2, 1/8, and 1/256 0.0% were detected in aborted ewes and in ramsrespectively.

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