Epidemiological survey of classical swine fever in Tibetan pigs in Nyingchi, Tibet China

2016 ◽  
Author(s):  
Jia-Kui Li ◽  
Hui Zhang ◽  
Peng Shang ◽  
Yangzom Chamba
Keyword(s):  
Classical Swine Fever ◽  
Epidemiological Survey ◽  
Economic Losses ◽  
Serum Samples ◽  
Immune Effect ◽  
Tibetan Pigs ◽  
Significant Difference ◽  
Long Time ◽  
The Government

Classical swine fever (CSF) is a major hazardous disease to the pigs and as a dangerous epidemic; it causes a serious economic losses to the pig industry. Though, a national compulsory immunization of CSF vaccines had been carried out for a long time, scarce information can be got about the immune effect of CSF in Tibetan pigs. The present study was to investigate the seroprevalence of CSF in Tibetan pigs in Nyingchi area of Tibet, China. A total 454 samples were collected from November 2014 to January 2015 and were investigated through enzyme-linked immuno sorbent assay (ELISA). The results showed that 241 (53.1%, 95% CI 48.4-57.8) pigs were found to be positive for CSF with the further distribution of 53.3% (95% CI 46.8–59.6), 49.5% (95% CI 42.2-56.8) and 93.8% (95% CI 69.8-99.8) in Tibetan counties of Nyingchi, Mainling and Gongbo'gyamda, respectively. There was no significant difference in male (52.8%, 95% CI 46.4-59.1) and female pigs (50.0%, 95% CI 42.6-57.4). Though, 53.1% of the serum samples were tested out positive to CSF, only the seroprevalence of CSF in Tibetan pigs in Gongbo'gyamda were higher than 70% which was ruled by the government. The low seroprevalence of CSF in Tibetan pigs should arise a serious concern and effective methods should be taken, in order to prevent CSF infection effectively.

scholarly journals Serodynamic Analysis of the Piglets Born from Sows Vaccinated with Modified Live Vaccine or E2 Subunit Vaccine for Classical Swine Fever

Pathogens ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 427 ◽  
Author(s):  
Yi-Chia Li ◽  
Ming-Tang Chiou ◽  
Chao-Nan Lin
Keyword(s):  
Detection Rate ◽  
Time Window ◽  
Classical Swine Fever ◽  
Significant Negative Correlation ◽  
Live Vaccine ◽  
Economic Losses ◽  
Respiratory Syndrome Virus ◽  
Serum Samples ◽  
Significant Difference ◽  
Modified Live Vaccine

Classical swine fever (CSF) caused by the CSF virus (CSFV) is one of the most important swine diseases, resulting in huge economic losses to the pig industry worldwide. Systematic vaccination is one of the most effective strategies for the prevention and control of this disease. Two main CSFV vaccines, the modified live vaccine (MLV) and the subunit E2 vaccine, are recommended. In Taiwan, CSF cases have not been reported since 2006, although systemic vaccination has been practiced for 70 years. Here, we examined the sero-dynamics of the piglets born from sows that received either the CSFV MLV or the E2 vaccine and investigated in the field the correlation between the porcine reproductive and respiratory syndrome virus (PRRSV) loads and levels of CSFV antibody. A total of 1398 serum samples from 42 PRRSV-positive farms were evaluated to determine the PRRSV loads by real-time PCR and to detect CSFV antibody levels by commercial ELISA. Upon comparing the two sow vaccination protocols (CSFV MLV vaccination at 4 weeks post-farrowing versus E2 vaccination at 4–5 weeks pre-farrowing), the lowest levels of CSFV antibody were found in piglets at 5–8 and 9–12 weeks of age for the MLV and E2 groups, respectively. Meanwhile, the appropriate time window for CSFV vaccination of offspring was at 5–8 and 9–12 weeks of age in the MLV and E2 groups, respectively. There was a very highly significant negative correlation between the PRRSV load and the level of CSFV antibody in the CSFV MLV vaccination group (P < 0.0001). The PRRSV detection rate in the pigs from the MLV group (27.78%) was significantly higher than that in pigs from the E2 group (21.32%) (P = 0.011). In addition, there was a significant difference (P = 0.019) in the PRRSV detection rate at 5–8 weeks of age between the MLV (42.15%) and E2 groups (29.79%). Our findings indicate that the vaccination of CSFV MLV in piglets during the PRRSV susceptibility period at 5–8 weeks of age may be overloading the piglet’s immune system and should be a critical concern for industrial pork production in the field.

scholarly journals Serological Investigation of Peste Des Petits Ruminants in East Shewa and Arsi Zones, Oromia Region, Ethiopia

2017 ◽  
Vol 2017 ◽  
pp. 1-5 ◽  
Author(s):  
Getachew Gari ◽  
Biressaw Serda ◽  
Dejene Negesa ◽  
Fethu Lemma ◽  
Hagos Asgedom
Keyword(s):  
Significant Variation ◽  
Small Ruminants ◽  
Control Measures ◽  
Economic Losses ◽  
Enzyme Linked Immunosorbent Assay ◽  
High Seroprevalence ◽  
Serum Samples ◽  
Male And Female ◽  
Significant Difference ◽  
Important Disease

Peste des petits ruminant (PPR) is an economically important disease of small ruminants with a rapidly expanding geographical distribution. There are fragmented reports to the occurrence and distribution of the disease in Ethiopia. A total of 700 serum samples were collected from goats and sheep to detect the presence of antibody against PPR virus using Competitive Enzyme-Linked Immunosorbent Assay (C-ELISA). An overall PPR seropositivity was reported to be 48.43% in the area. There is no statistically significant difference in the seroprevalence of the disease between sheep and goats (50.85% and 46.68%), respectively. However, there was statistically significant variation (P<0.05) in the seroprevalence of the disease in young (33.9%) and adult (55.8%) age categories. The seroprevalence in male and female was 42.07% and 50.09%, respectively, where the variation was statistically not significant (P>0.05). High seroprevalence of Peste des petites ruminants in the study area indicated the virus circulation and endemicity of the disease. The disease causes substantial economic losses by affecting the livelihood of the farmers. Therefore, control measures should be put in place to minimize the loss associated with the disease.

scholarly journals Longitudinal Changes in the Government-Certified Index Stage and Requisite Costs for Long-Term Care Insurance System among the Community-Dwelling Demented Elderly in Japan

2013 ◽  
Vol 2013 ◽  
pp. 1-6
Author(s):  
Shunichiro Shinagawa ◽  
Shiori Nakamura ◽  
Makoto Iwamoto ◽  
Norifumi Tsuno ◽  
Masahiro Shigeta ◽  
...  
Keyword(s):  
Long Term Care ◽  
Epidemiological Survey ◽  
Community Dwelling ◽  
Clinical Dementia Rating ◽  
Term Care ◽  
Dementia Patients ◽  
Significant Difference ◽  
The Government

Background. A new public long-term care (LTC) insurance was launched in 2000 in Japan. However, there have been few studies involving factors that increase LTC costs of demented subjects; no follow-up studies involving the Government-Certified Index (GCI) and requisite costs related to the causes of dementia.Method. An epidemiological survey was conducted in a rural area in Japan in 1999, and 271 subjects were diagnosed as dementia patients. Age, sex, mini-mental state examination, clinical dementia rating, activity of daily living, causes of dementia, and coexisting physical disease were confirmed. After the LTC insurance has been launched, we tracked the GCI stages and payment amounts every month for 8 years.Result. 209 subjects were certified to be eligible for LTC insurance; however, 13 did not receive any payment. Only 49 out of 209 were alive after the follow-up period. The most common cause of dementia was Alzheimer’s disease (AD), followed by vascular dementia (VaD). There was no significant difference between the mortality rates of the two groups. VaD subjects required higher costs than AD subjects in the total certified period and in GCI stage 5.Conclusion. Our results indicate that causes of dementia can have an impact on the requisite costs for the LTC insurance.

scholarly journals Q fever in the Netherlands: a sero-epidemiological survey among human population groups from 1968 to 1983

1987 ◽  
Vol 98 (2) ◽  
pp. 211-219 ◽  
Author(s):  
J. H. Richardus ◽  
A. Donkers ◽  
A. M. Dumas ◽  
G. J. P. Schaap ◽  
J. P. W. M. Akkermans ◽  
...  
Keyword(s):  
The Netherlands ◽  
Indirect Immunofluorescence ◽  
Q Fever ◽  
Epidemiological Survey ◽  
Immunofluorescence Test ◽  
Age Classes ◽  
Serum Samples ◽  
Occupational Groups ◽  
Indirect Immunofluorescence Test ◽  
Long Time

SUMMARYA sero-epidemiological survey, using an indirect immunofluorescence test for IgG against Coxiella burnetii (phase II), was carried out in the Netherlands. Serum samples taken in 1968, 1975, 1979 and 1983 were tested. Occupational groups with a supposedly high risk of infection (veterinarians, residents of dairy farms and taxidermists) showed a significantly higher percentage of seropositives than defined controls. The percentage of seropositive amateur wool spinners was significantly higher than that of the controls from the same region. Since 1968 there has been no increase in the percentage of infected persons, indicating that, contrary to earlier assumptions, Q fever has been endemic in The Netherlands for a long time already. The increase in numbers of notified cases of overt Q fever is considered to be the result of the recent introduction of a sensitive indirect immunofluorescence test for IgM antibodies against C. burnetii. Antibody percentages in all age classes between 1 and 64 years were much alike, suggesting that most infections occur in early childhood. This is in accordance with the finding that 35% of our patients are younger than 3 years. The possibility of infection related to childbirth and lactation is discussed.

scholarly journals Prevalence of Porcine Pseudorabies Virus and Its Coinfection Rate in Heilongjiang Province in China from 2013 to 2018

2019 ◽  
Author(s):  
Han Zhou ◽  
Yan Pan ◽  
Mengda Liu ◽  
Zifeng Han
Keyword(s):  
Pseudorabies Virus ◽  
Classical Swine Fever ◽  
Porcine Circovirus ◽  
Economic Losses ◽  
Respiratory Syndrome Virus ◽  
Heilongjiang Province ◽  
Serum Samples ◽  
Tissue Samples ◽  
Wide Range ◽  
Pig Farms

Abstract Background Pseudorabies (PR) is a highly contagious disease affecting a wide range of animals worldwide, which causes great annual economic losses in China. In this study, a total number of 18815 serum samples and 1589 tissue samples were tested by ELISA and PCR, which were collected from 311 intensive pig farms (≥350 sows) located in eight cities in Heilongjiang province. Results Overall, 16.3% and 84.5% serum samples were positive for gE- and gB-specified ELISA, respectively. 17.8% of tissue samples were shown as PCR positive for PRV. The co-infection rates of PRV with porcine reproductive and respiratory syndrome virus (PRRSV), porcine circovirus type 2 (PCV2), and classical swine fever virus (CSFV) were measured. The rate of PRV co-infected with PRRSV was 36.0% followed by 12.9% with PCV2 and 1.8% with CSFV, respectively. Conclusions These results clearly demonstrate PRV prevalence and its coinfection rate in Heilongjiang province indicating high PR endemic in pig farms in this region. This study provides data for further epidemiological investigations and a reference for developing PRV control strategies in this region and in China.

scholarly journals Inactivation of Classical Swine Fever Virus in Porcine Serum Samples Intended for Antibody Detection

Pathogens ◽  
2019 ◽  
Vol 8 (4) ◽  
pp. 286 ◽  
Author(s):  
Denise Meyer ◽  
Anja Petrov ◽  
Paul Becher
Keyword(s):  
Neutralization Test ◽  
Classical Swine Fever ◽  
Antibody Detection ◽  
Economic Losses ◽  
Pragmatic Approach ◽  
Valuable Contribution ◽  
Serum Samples ◽  
Diagnostic Assays ◽  
Porcine Serum ◽  
Phosphate Buffered Saline

Shipping of serum samples that were taken from pigs infected with classical swine fever (CSF) virus is frequently requested with the objective of serological analyses, not only for diagnostic purposes but also for exchange of reference materials that are used as control material of diagnostic assays. On the basis of the fact that an outbreak with CSF is associated with enormous economic losses, biological safety during the exchange of reference material is of great importance. The present study aimed to establish a pragmatic approach for reliable CSF virus (CSFV) inactivation in serum without impairing antibody detection. Considering the fact that complement inactivation through heating is routinely applied, the basic idea was to combine heat treatment with the dilution of serum in a detergent containing buffer in order to facilitate the inactivation process. The results show that treatment of serum samples with phosphate buffered saline-Tween20 (final concentration = 0.15%) along with incubation at 56 °C for 30 min inactivated CSFV and such treatment with ≤ 0.25% PBS-Tween20 does not impair subsequent antibody detection by ELISA or virus neutralization test. This minimizes the risk of virus contamination and represents a valuable contribution to a safer CSF diagnosis on a national and international level.

scholarly journals Seroprevalences of classical swine fever virus and porcine reproductive and respiratory syndrome virus in pigs in Hunan Province, China

2020 ◽  
Author(s):  
Haoyang Yu ◽  
Luhua Zhang ◽  
Yunfeng Cai ◽  
Tao Peng ◽  
Lei Liu ◽  
...  
Keyword(s):  
Average Rate ◽  
Classical Swine Fever ◽  
Control Measures ◽  
Hunan Province ◽  
Economic Losses ◽  
Respiratory Syndrome Virus ◽  
Growth Stages ◽  
Serum Samples ◽  
Swine Industry ◽  
Effective Prevention

Abstract Background: Classical swine fever (CSF) and porcine reproductive and respiratory syndrome (PRRS) are responsible for major economic losses and represent a threat to the swine industry worldwide. Routine surveillance serology for CSF and PRRS will be critical to maintain the health status of sow farms in Hunan Province, which is one of the top pig production provinces in China.The objective of our study was to investigate the seroprevalence of CSF virus (CSFV) and PRRS virus (PRRSV) in Hunan Province from 2017 to 2019 using ELISA. The cohort pig serum samples were collected from vaccinated and unvaccinated sow farms in eight cities in Hunan Province. Results: CSFV and PRRSV seropositivity showed significant differences among parts of Hunan Province in China. Interestingly, our findings show that the level of CSFV antibody in piglet sera decreases gradually with increasing piglet age, whereas PRRSV-specific antibodies may appear in various growth stages in unvaccinated animals. Meanwhile, the average rate of CSFV antibody seropositivity was 82.2%, while the rate of PRRSV antibody seropositivity was slightly higher (84.4%) in the immunized group.Conclusions: The results from this study suggest that a serological investigation of these two major infectious diseases (CSF and PRRS) among pigs at various growth stages will provide a basis for the further development of more effective prevention and control measures.

scholarly journals Efficiency Comparison of a Novel E2 Subunit Vaccine and a Classic C-Strain Vaccine against Classical Swine Fever

2021 ◽  
Vol 8 (8) ◽  
pp. 148
Author(s):  
Pei Zhou ◽  
Junming Huang ◽  
Yanchao Li ◽  
Hui Chen ◽  
Yidan Wu ◽  
...  
Keyword(s):  
Subunit Vaccine ◽  
Classical Swine Fever ◽  
Antibody Responses ◽  
Control Measures ◽  
Economic Losses ◽  
Swine Industry ◽  
Field Virus ◽  
Strain Vaccine ◽  
Efficiency Comparison ◽  
The Government

Classical swine fever (CSF) is one of the most important viral diseases in swine, causing severe economic losses in the swine industry. In China, CSF is one of the key diseases that needs to be controlled; the government has implemented control measures, and vaccination with C-strain vaccines (C-vacs) has been compulsory since the 1950s. C-vacs do not allow the differentiation of field virus-infected and vaccinated animals (DIVA). In 2012, China proposed a goal of eradicating CSF. Additionally, a baculovirus-expressed E2 subunit vaccine (E2-vac) was licensed in 2018. However, the C-vac and E2-vac characteristics have not been compared. Here, we demonstrate that both the C-vac and E2-vac provide complete protection against CSF in pigs. The E2-vac allows DIVA, and the E2 antibody responses of stimulated pigs are developed earlier and are stronger than the C-vac antibody responses. Therefore, the E2-vac is a new candidate licensed vaccine to completely eradicate CSF on pig farms.

Determination of Blood Serum Protein Fractions of Calves with Clinically Diagnosed Pneumonia

2020 ◽  
Author(s):  
Pınar Tanritanir Ekici ◽  
Semiha Dede ◽  
Veysel Yüksek ◽  
Sedat Çetin ◽  
Ayşe Usta
Keyword(s):  
Serum Protein ◽  
Patient Group ◽  
Total Protein ◽  
Protein Fractions ◽  
Economic Losses ◽  
Blood Parameter ◽  
Serum Samples ◽  
Protein Levels ◽  
Significant Difference ◽  
G Ratio

Background: Respiratory diseases in calves are causing worldwide economic losses for the beef industry. This study was planned to determine the serum protein fractions and A/G ratio in calves with clinically diagnosed pneumonia, and evaluate the possibility uses of these parameters as clinical diagnostic parameters.Methods: The 34 calves with respiratory system problems and 10 healthy calves without clinical pneumonia symptoms were used as materials. The obtained serum samples were used for total protein and serum protein electrophoresis.Result: Albumin, α1-globulin percentages and A/G ratio decreased in the patient group (p≤0.01), other globulin fractions were higher in the patient group (p≤0.01), for % g values. According to concentration results, it was found that while albumin did not show a significant difference, α1-globulin (p≤0.05) and A / G ratio (p≤0.01) decreased in the patient group. In addition, other globulin fractions and total protein levels were higher in patient groups (p≤0.01). As a result, the serum protein fractions should be evaluated as a useful biochemical blood parameter for diagnosis and follow up of lung diseases especially in calves.

scholarly journals Socio-economic outcomes of the COVID-19 pandemic for Ukraine

2021 ◽  
pp. 24-42
Author(s):  
VITALINA BUTKALIUK
Keyword(s):  
Well Being ◽  
Economic Losses ◽  
National Academy Of Sciences ◽  
Systemic Analysis ◽  
Sociological Inquiry ◽  
Long Time ◽  
Research Findings ◽  
The Government ◽  
Academy Of Sciences ◽  
Public Views

The article presents a comprehensive analysis of the causes and consequences of both human and socio-economic losses incurred by Ukraine as a result of the COVID-19 pandemic and restrictions introduced by the government in order to prevent its spread. The author's attention is primarily focused on exploring the population's social well-being, as well as examining changes in the quality of Ukrainians' lives during the pandemic. The study of the above-mentioned issues is complemented by a sociological inquiry into public views on the coronavirus problem, efficacy of the authorities' actions aimed at combating the pandemic, as well as the essence and real effectiveness of socio-economic policy pursued by the Ukrainian government in present-day conditions. Drawing upon a systemic analysis, the author argues that the ongoing socio-economic crisis in Ukraine, along with vaccine crunch, stems mainly from neoliberal policies implemented by the national government over the past three decades. The article argues the thesis about the transformation of the crisis of confidence, which has been fixed for a long time in Ukraine, into a crisis of vaccination, which today threatens with large humanitarian, economic and geopolitical losses. The research findings allow concluding that the COVID-19 pandemic acted as another trigger for the crisis, thereby intensifying and exacerbating the problems that had already existed in the national economy. The author's arguments are bolstered by a vast array of domestic and foreign statistical data, along with the results of surveys conducted by the Institute of Sociology of the National Academy of Sciences of Ukraine (1994–2020), «Research & Branding Group» (2020–2021), as well as other Ukrainian sociological centers.

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