scholarly journals Problems of mini-pig breeding

2021 ◽  
Vol 25 (3) ◽  
pp. 284-291
Author(s):  
K. S. Shatokhin
Keyword(s):  
Genetic Diversity ◽  
Coat Color ◽  
Molecular Genetic ◽  
Harmful Effect ◽  
Live Weight ◽  
Laboratory Animals ◽  
Selection Strategy ◽  
Breeding Groups ◽  
Mini Pigs ◽  
Close Inbreeding

This article provides an overview of some problems of the breeding and reproduction of laboratory minipigs. The most obvious of these are the lack of centralized accounting of breeding groups, uniform selection standards for reproduction and evaluation of breeding animals, as well as minimizing the accumulation of fitness-reducing mutations and maintaining genetic diversity. According to the latest estimates, there are at least 30 breeding groups of mini-pigs systematically used as laboratory animals in the world. Among them, there are both breed formations represented by several colonies, and breeding groups consisting of a single herd. It was shown that the main selection strategy is selection for the live weight of adults of 50–80 kg and the adaptation of animals to a specific type of biomedical experiments. For its implementation in the breeding of foreign mini-pigs, selection by live weight is practiced at 140- and 154-day-old age. It was indicated that different herds of mini-pigs have their own breeding methods to counteract inbred depression and maintain genetic diversity. Examples are the maximization of coat color phenotypes, the cyclical system of matching parent pairs, and the structuring of herds into subpopulations. In addition, in the breeding of foreign mini-pigs, molecular genetic methods are used to monitor heterozygosity. Every effort is made to keep the number of inbred crosses in the breeding of laboratory mini-pigs to a minimum, which is not always possible due to their small number. It is estimated that to avoid close inbreeding, the number of breeding groups should be at least 28 individuals, including boars of at least 4 genealogical lines and at least 4 families of sows. The accumulation of genetic cargo in herds of mini-pigs takes place, but the harmful effect is rather the result of erroneous decisions of breeders. Despite the fact that when breeding a number of mini-pigs, the goal was to complete the herds with exclusively white animals, in most breeding groups there is a polymorphism in the phenotype of the coat color.

Prenatal growth of live weight and coat color of the mini pigs of ICG SB RAS

2019 ◽  
Vol 1 (3) ◽  
pp. 21-34
Author(s):  
Sergei V. Nikitin ◽  
Sergei P. Knyazev ◽  
Kirill S. Shatokhin ◽  
Vera I. Zaporozhets ◽  
Dar`ya S Bashur ◽  
...  
Keyword(s):  
Coat Color ◽  
Relative Weight ◽  
Live Weight ◽  
High Mass ◽  
Prenatal Period ◽  
Large White ◽  
Average Mass ◽  
Prenatal Growth ◽  
The Individual ◽  
Mini Pigs

The article considers the possibility of connection of KIT and MC1R loci with prenatal growth of mini pigs of the breeding group of Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences (ICG SB RAS). It is assumed that this relationship may be the result of increased variability of the sign – the mass of the newborn pig in heterozygous according to the loci of individuals. Since prenatal growth is affected by a number of factors that affect the weight of the newborn pig, instead of the absolute value of the sign – the mass of the individual in kilograms, a relative one was used, which is the ratio of the mass of a particular individual to the average mass of the individual in the nest in which it was born. The study showed that mini-pigs of ICG SB RAS have a relationship between heterozygosity at MC1R and KIT loci and increased concentration of individuals in the modal class of relative weight distribution at birth. The discovered relationship is the result of the interaction of increased variability of mass at birth in individuals’ heterozygous loci, controlling intracellular metabolism, with the vector of phenotypic realization of the genetic potential of growth of individuals in the prenatal period. The decomposition of the specified vector into components showed that optimizes forming the kurtosis variation of the number of components dominate in individuals with domestication coat color, while in individuals with pseudoagouti coat color – biasing, forming the asymmetry of the distribution. The results of the study also showed that the genotypes at the loci MC1R and KIT do not affect the competitive relationship between the fetuses during the prenatal period . In addition, the analysis of the consistency of the estimates of individual classes of variational series of individuals of different races to a normal distribution showed that the mini pigs of ICG SB RAS alleles present low weight at birth, inherited from the Vietnamese breeds , and alleles of the high mass at birth, inherited from the Large White and/or Danish Landrace breed.

Molecular Genetic Dissection of Mouse Unconventional Myosin-VA: Tail Region Mutations

Genetics ◽  
1998 ◽  
Vol 148 (4) ◽  
pp. 1963-1972 ◽  
Author(s):  
Jian-Dong Huang ◽  
Valerie Mermall ◽  
Marjorie C Strobel ◽  
Liane B Russell ◽  
Mark S Mooseker ◽  
...  
Keyword(s):  
Coat Color ◽  
Molecular Genetic ◽  
Genetic Dissection ◽  
Rt Pcr ◽  
Tail Region ◽  
Unconventional Myosin ◽  
Myosin Va ◽  
Cell Type Specific ◽  
Extensive Collection ◽  
Tail Function

AbstractWe used an RT-PCR-based sequencing approach to identify the mutations responsible for 17 viable dilute alleles, a mouse-coat-color locus encoding unconventional myosin-VA. Ten of the mutations mapped to the MyoVA tail and are reported here. These mutations represent the first extensive collection of tail mutations reported for any unconventional mammalian myosin. They identify sequences important for tail function and identify domains potentially involved in cargo binding and/or proper folding of the MyoVA tail. Our results also provide support for the notion that different myosin tail isoforms produced by alternative splicing encode important cell-type-specific functions.

Evaluation Yorkshire and Landrace breeds of pigs according to PRKAG3, MC4R and MYOD1 marker genes

2021 ◽  
pp. 28-35
Author(s):  
A. Balnikov ◽  
I. Gridyushko ◽  
Yu. Kazutova ◽  
M. Mikhailova ◽  
E. Romanishko
Keyword(s):  
Molecular Genetic ◽  
Live Weight ◽  
Integrated System ◽  
The Body ◽  
Breeding Value ◽  
Feed Consumption ◽  
State Enterprise ◽  
Marker Genes ◽  
Productive Traits ◽  
Meat Productivity

Purpose: evaluation of pigs Yorkshire rocks and Landraz on PRKAG3, MC4R and MyOD1 markers.Materials and methods. The paper presents the analysis of the results of genetic testing, and determines the frequency of alleles and genotypes of Yorkshire and Landrace breeds at the State Enterprise ZhodinoAgroPlemElita" of the Minsk region of the Republic of Belarus by the genes-markers of productive traits: PRKAG3, MC4R and MYOD1. Zootechnical and molecular genetic methods were used in the research (PCR/PDRF). Snapshot analysis was developed for the genotyping of pigs to detect polymorphic markers of meat-feeding productivity of pigs on the Genas PRKAG3, MyOD1, MC4R. At the first stage, there was an assessment of tribal young (173 heads) on its own productivity in terms of indicators: the age of achieving the living mass of 100 kg, the average daily increase (d) from birth to the live weight of 100 kg, the length of the body (cm), the lifetime meat quality of the thickness of the scrape (mm), height of the longest back muscles (mm), the content of meat in the body (%). At the second stage of the research, the controlling fastenings of 200 goals of pigs and evaluation of feedst and meat qualities in the following indicators were carried out: the age of achieving a living mass of 100 kg (days), the average daily increase (d), feed consumption per 1 kg of growth (to. Units). Also determined: the length of the carcass (cm), the slaughter output (%), the thickness of the spick over 6-7 breast vertebrae (mm), the area of "muscular eye" (cm2), the mass of the rear third of the half carcass (kg), the flow of meat in the carcus (%). Then, genetic studies were compared with productivity indicators.Results. As a result of the research, it was found that in the tested animals, the frequency of the preferred allele I-PRKAG3 made 0.171-0.288, A-MC4R – 0.243-0.315, and C-MYOD1 – 0.064-0.477, respectively. The developed comprehensive system for assessing the breeding value of Yorkshire and Landrace pigs based on the marker genes PRKAG3, MC4R, and MYOD1 allowed us to identify the preferred alleles and determine correlation with productivity traits that provided increase in the average population values: for self-productivity - by 2-4%, for fattening traits - by 2.7-12.5%, for meat traits - by 2.8-34.4 %.Conclusion. The use of integrated system in pig breeding will speed up the selection work to increase the indicators of fattening and meat productivity of the created breeding herds of Yorkshire and Landrace pigs.

scholarly journals Molecular Characterization and Similarity Relationships among Flue-Cured Tobacco (Nicotiana tabacum L.) Genotypes Using Simple Sequence Repeat Markers

2012 ◽  
Vol 40 (2) ◽  
pp. 247
Author(s):  
Soheila GHOLIZADEH ◽  
Reza DARVISHZADEH ◽  
Babak ABDOLLAHI MANDOULAKANI ◽  
Iraj BERNOUSI ◽  
Seyed Reza ALAVI ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Molecular Genetic ◽  
Genetic Distances ◽  
Similarity Coefficients ◽  
Allele Number ◽  
Nicotiana Tabacum L ◽  
Ssr Loci ◽  
Actual Degree ◽  
Similarity Relationships

Characterization of genetic diversity has long been a major goal in tobacco breeding programs. Information on genetic diversity is essential for a rational use of genetic resources. In the present study, the genetic variation among 72 flue-cured tobacco genotypes was evaluated using microsatellite markers (SSRs). A set of 104 alleles was generated at 30 SSR loci. The mean number of alleles per locus (na) and the effective allele number (ne) were 3.467 and 2.358, respectively. The expected heterozygosity ranged from 0.29 to 0.75 with average of 0.54. Several methods were used to construct the similarity matrices and dendrograms. The co-phenetic correlation coefficient, which is a measure of the correlation between the similarities represented on the dendrograms and the actual degree of similarity, was calculated for each dendrogram. Among the different methods, the highest value (r=0.76368) was observed for the UPGMA created based on Jaccard’s similarity coefficients. The genetic similarity among the tobacco genotypes calculated by using Jaccard’s similarity coefficient ranged from 0.08 to 0.84, suggesting the presence of high molecular genetic variability among the studied tobacco genotypes. Based on UPGMA clustering method all studied flue-cured tobacco genotypes, except for ‘Glustinusa Rasht’, were placed in three distinct groups. We observed an obvious heterotic pattern in the studied flue-cured germplasm corresponding to genetic distances and classification dendrogram, which persuades exploitation of heterosis in flue-cured tobaccos.

Study on molecular genetic diversity of native duck breeds in China

2006 ◽  
Vol 62 (04) ◽  
pp. 603-611
Author(s):  
H. LI ◽  
N. YANG ◽  
K. CHEN ◽  
G. CHEN ◽  
Q. TANG ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Molecular Genetic ◽  
Molecular Genetic Diversity

scholarly journals Test Procedure for a New Type of a Ventricular Assist Device Based on a Viscous Friction Pump

2020 ◽  
Vol 13 (3) ◽  
pp. 256-261
Author(s):  
Maksim Zhulkov ◽  
Alexander Golovin ◽  
Alexander Grenaderov ◽  
Vitaliy Tsirikhov ◽  
Azat Sabetov ◽  
...  
Keyword(s):  
Ventricular Assist Device ◽  
Test Procedure ◽  
Viscous Friction ◽  
Left Ventricular ◽  
Ventricular Assist Devices ◽  
Laboratory Animals ◽  
Implantation Technique ◽  
Assist Device ◽  
Ventricular Assist ◽  
Mini Pigs

Introduction. Currently, the possibility of effective correction of multiple organ failure and increasing the life expectancy of patients with critical heart failure using the ventricular assist devices (VAD) is absolutely evidenced. The development of alternative analogues of such devices produced within the country has been an urgent problem for many decades. The design of this machine requires the development of a protocol for carrying out complex biomedical studies on the biocompatibility and safety of the new device.The aim of the study was to develop a protocol for testing and conducting comprehensive biomedical biocompatibility studies of a new ventricular assist device in an acute animal experiment.Materials and methods. The study to develop a technique for implanting a left-ventricular assist device (LVAD) in an acute experiment included female mini-pigs, weighed 40-60 kg. In a series of acute experiments, 5 implantations of a disk pump as a LVAD were performed with a maximum observation period equal 6 hours.Results. In the series of acute experiments, an implantation technique was developed and the fundamental possibility of a viscous friction pump application as a ventricular assist device was evidenced. In all experiments (n = 5), the average level of free hemoglobin did not exceed 2.6 mg%, which supports safety of performance of the viscous friction pump regarding blood erythrocytes. None of the experiments recorded episodes of pump shut-off or breakdown.Conclusions. The series of acute experiments on mini-pigs helped reveal a number of anatomical and physiological features of this animal species that significantly complicated implementation of persistent observation. However, the developed experimental LVAD test methodology can be recommended for use in further chronic experiments on large laboratory animals (calves).

scholarly journals SELECTION EFFICIENCY OF INBRED CATTLE OF UKRAINIAN WHITEHEAD BREED

2020 ◽  
pp. 232-245
Author(s):  
Svitlana Voitenko ◽  
Olena Sydorenko
Keyword(s):  
Milk Yield ◽  
Local Population ◽  
Live Weight ◽  
Moderate Degree ◽  
Absolute Increase ◽  
Reproductive Ability ◽  
Group I ◽  
Significant Difference ◽  
Close Inbreeding ◽  
Selection Of

The stated results of using outbreeding and inbreeding in a selection of cattle of the Ukrainian Whitehead breed, which belongs to the local population and is preserved only in one breeding herd. It was revealed that animals obtained from related breeding are characterized by a sufficiently high level of productivity and normal reproductive qualities. Although outbred and inbred heifers in the process of growth from birth to 18 months of age differed somewhat from each other in live weight, the difference was not statistically significant. In different periods of growth, both outbred and inbred animals had an advantage, indicating the possibility of improving the trait by purebred breeding methods. The absolute increase in live weight of experimental heifers on rearing was: Group I – 295 kg, II – 289 kg, III – 298 kg, IV – 308 kg and V – 293 kg without a statistically significant difference between the groups, which made it possible to conclude that there was no inbred depression when breeding cattle of a given herd through a related selection of parental pairs. It is recognized that the selection of calves to increase their live weight at birth will not have a positive effect on the trait during the growth of animals, as evidenced by the correlation between them. As a result of the study of reproductive ability, the expediency of obtaining and using inbred cattle in a herd, which was inseminated almost a month earlier than outbred, was proved. Between outbred and inbred cows, a significant differentiation of milk yield for the first – third lactation was found, without a significant advantage of animals obtained by one or another method of purebred breeding. According to the first lactation, the highest milk yield was observed in cows of the close inbreeding group (group V) – 4501 kg, which exceeded the outbred ones by 150 kg and the lower inbred ones (groups II-IV) by 191 – 633 kg. Inbred cattle with distant, moderate and close degrees of inbreeding at the second lactation had from 4629 kg to 4719 kg of milk, with the highest rate in cows of a moderate degree of inbreeding, while outbred cows produced only 4582 kg. Cows of a moderate degree of inbreeding had the highest milk yield in the third lactation – 5204 kg and the lowest – 3897 kg of the group of a close degree of inbreeding. Outbred cows for this trait were superior only to individuals of the group of close inbreeding. The results of a comparative analysis of economically useful traits of outbred and inbred cows and heifers of the Ukrainian Whitehead breed indicate the possibility of using a related selection of parental pairs in a herd to increase the efficiency of interbreeding selection, replicate the hereditary traits of the ancestor and preserve the disappearing domestic breed of cattle.

scholarly journals Use of water-alcohols treatment of medicinal plants under conditions modeling intoxication by lead and cadmium

2021 ◽  
pp. 80-88
Author(s):  
Yu. I. Koval ◽  
T. I. Bokova ◽  
A. F. Petrov
Keyword(s):  
Heavy Metals ◽  
Medicinal Plants ◽  
Blood Serum ◽  
Raw Materials ◽  
Antioxidant Properties ◽  
Live Weight ◽  
The Body ◽  
Laboratory Animals ◽  
Lead And Cadmium ◽  
Water Alcohol

The results of studying the effects of water-alcohol infusions of medicinal plants - rhizomes of thick-leafed incense (Bergenia crasslifolia L. Fritsch), dioica nettle leaves (Urtica dioca L.), burdock roots (Arctium lappa L.), and mother-and-leaf leaves - are presented. ordinary machichi (Tussilago farfara L.) on the organism of laboratory rats under conditions of short-term intoxication with heavy metals in doses of 25 mg of lead and 2.5 mg of cadmium per 1 kg of live weight. In the course of studies it was found that the effect of heavy metals on the body of laboratory animals is selective - the main “depot” of lead was bone tissue, and cadmium - the kidneys and liver. The addition of 1 ml of water-alcohol infusions from medicinal raw materials to the diet of rats as detoxicants caused a decrease in the level of lead in the animal organism to 6.32 times, cadmium to 3.46 times. The greatest detoxification ability was shown by infusions of the roots of burdock and leaves of nettle dioecious. Under the influence of elevated doses of lead and cadmium in laboratory animals there was an increase in heart mass by 40.34 % and spleen by 89.91 %, a decrease in the content of alkaline phosphatase in blood serum by 25.81 %. Water-alcohol infusions normalized the biochemical parameters of rat blood serum. Studies have shown that the infusions of rhizomes of thick-leafed frangipani, burdock roots, dioecious nettles and coltsfoot leaves with antioxidant properties can be the basis for the development of an effective herbal preparation used for the prevention and treatment of animals from intoxication with salts of heavy metals.

scholarly journals The pattern of genetic diversity of different breeds of pigs based on microsatellite analysis

2020 ◽  
Vol 24 (7) ◽  
pp. 747-754
Author(s):  
V. R. Kharzinova ◽  
N. A. Zinovieva
Keyword(s):  
Genetic Diversity ◽  
Genetic Relationships ◽  
Molecular Genetic ◽  
Genetic Material ◽  
Animal Husbandry ◽  
Molecular Genetic Analysis ◽  
Fixation Index ◽  
Large White ◽  
Black And White ◽  
The Republic

One of the main tasks of genetics and animal breeding is the assessment of genetic diversity and the study of genetic relationships between different breeds and populations using molecular genetic analysis methods. We analysed the polymorphism of microsatellites and the information on the state of genetic diversity and the population structure of local breeds in Russia: the Kemerovo, the Berkshire, the Liven, the Mangalitsa, and the Civilian; in the Republic of Belarus: the Large White and the Black-and-White; and in Ukraine: the White Steppe, as well as commercial breeds of imported origin of domestic reproduction: the Large White, the Landrace, and the Duroc. The materials used for this study were the tissue and DNA samples extracted from 1,194 pigs and DNA of the UNU “Genetic material bank of domestic and wild animal species and birds” of the L.K. Ernst Federal Research Center for Animal Husbandry. Polymorphisms of 10 microsatellites (S0155, S0355, S0386, SW24, SO005, SW72, SW951, S0101, SW240, and SW857) were determined according to the previously developed technique using DNA analyser ABI3130xl. To estimate the allele pool of each population, the average number of alleles (NA), the effective number of alleles (NE ) based on the locus, the rarified allelic richness (AR), the observed (HO ) and expected (HE ) heterozygosity, and the fixation index (FIS) were calculated. The degree of genetic differentiation of the breeds was assessed based on the pairwise values of FST and D. The analysis of the allelic and genetic diversity parameters of the local breeds showed that the maximum and minimum levels of polymorphism were observed in pigs of the Ukrainian White Steppe breed (NA = 6.500, NE = 3.709, and AR = 6.020) and in pigs of the Duroc breed (NA = 4.875, NE = 2.119, and AR = 3.821), respectively. The highest level of genetic diversity was found in the Large White breed of the Republic of Belarus (HO = 0.707 and NE = 0.702). The minimum level of genetic diversity was found in pigs of the imported breeds – the Landrace (HO = 0.459, HE = 0.400) and the Duroc (HO = 0.480, HE = 0.469) – indicating a high selection pressure in these breeds. Based on the results of phylogenetic analysis, the genetic origin of Large White pigs, the breeds, from which the Berkshire pigs originated, and the genetic detachment of the Landrace from the Mangalitsa breeds were revealed. The cluster analysis showed a genetic consolidation of the Black-and-White, the Berkshire, and the Mangalitsa pigs. Additionally, the imported breeds with clustering depending on the origin were characterised by a genetic structure different from that of the other breeds. The information obtained from these studies can serve as a guide for the management and breeding strategies of the pig breeds studied, to allow their better use and conservation.

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