scholarly journals An integrated method for taxonomic identif ication of microorganisms

2020 ◽  
Vol 24 (4) ◽  
pp. 376-382
Author(s):  
Yu. E. Uvarova ◽  
A. V. Bryanskaya ◽  
A. S. Rozanov ◽  
V. N. Shlyakhtun ◽  
E. A. Demidov ◽  
...  
Keyword(s):  
Molecular Genetic ◽  
Integrated Approach ◽  
Visual Observation ◽  
Phenotypic Characteristics ◽  
Rna Molecules ◽  
Integrated Method ◽  
Observation Methods ◽  
Genetic Techniques ◽  
Artificial Classification ◽  
Genotypic Characteristics

For accurate species-level identification of microorganisms, researchers today increasingly use a combination of standard microbiological cultivation and visual observation methods with molecular biological and genetic techniques that help distinguish between species and strains of microorganisms at the level of DNA or RNA molecules. The aim of this work was to identify microorganisms from the ICG SB RAS Collection using an integrated approach that involves a combination of various phenotypic and genotypic characteristics. Key molecular-genetic and phenotypic characteristics were determined for 93 microbial strains from the ICG SB RAS Collection. The strains were characterized by means of morphological, physiological, moleculargenetic, and mass-spectrometric parameters. Specific features of the growth of the strains on different media were determined, and cell morphology was evaluated. The strains were tested for the ability to utilize various substrates. The strains studied were found to significantly differ in their biochemical characteristics. Physiological characteristics of the strains from the collection were identified too, e. g., the relationship with oxygen, type of nutrition, suitable temperature and pH ranges, and NaCl tolerance. In this work, the microorganisms analyzed were combined into separate groups based on the similarities of their phenotypic characteristics. This categorization, after further refinement and expansion of the spectrum of taxa and their metabolic maps, may serve as the basis for the creation of an “artificial” classification that can be used as a key for simplified and quicker identification and recognition of microorganisms within both the ICG SB RAS Collection and other collections.

scholarly journals Integrated method for the measurement of trace atmospheric bases

2011 ◽  
Vol 4 (5) ◽  
pp. 6007-6040 ◽  
Author(s):  
D. Key ◽  
J. Stihle ◽  
J.-E. Petit ◽  
C. Bonnet ◽  
L. Depernon ◽  
...  
Keyword(s):  
Nitrogen Cycle ◽  
Field Measurements ◽  
Ambient Air ◽  
Integrated Approach ◽  
Urban Park ◽  
Sampling Strategies ◽  
Road Vehicles ◽  
Integrated Method ◽  
The Many ◽  
Global Nitrogen Cycle

Abstract. Nitrogenous atmospheric bases are thought to play a key role in the global nitrogen cycle, but their sources, transport, and sinks remain poorly understood. Of the many methods available to measure such compounds in ambient air, few meet the current need of being applicable to the complete range of potential analytes and fewer still are convenient to implement using instrumentation that is standard to most laboratories. In this work, an integrated approach to measuring trace atmospheric nitrogenous bases has been developed and validated. The method uses a simple acid scrubbing step to capture and concentrate the bases as their phosphite salts, which then are derivatized and analyzed using GC/MS and/or LC/MS. The advantages of both techniques in the context of the present measurements are discussed. The approach is sensitive, selective, reproducible, as well as convenient to implement and has been validated for different sampling strategies. The limits of detection for the families of tested compounds are suitable for ambient measurement applications, as supported by field measurements in an urban park and in the exhaust of on-road vehicles.

scholarly journals Microsatellite Genetic Markers of Saccharrum spp., and Erianthus sp. on Their Hybrids

2019 ◽  
Vol 3 (1) ◽  
pp. 1
Author(s):  
Mala Murianingrum ◽  
Taryono Taryono ◽  
Rani Agustina Wulandari
Keyword(s):  
Molecular Marker ◽  
Genetic Marker ◽  
Genetic Markers ◽  
Chromosome Segregation ◽  
Molecular Genetic ◽  
Female Parent ◽  
Limiting Factors ◽  
Phenotypic Characteristics ◽  
Saccharum Spp ◽  
Sugarcane Varieties

Progeny identification is the important step that should be done after hybridization. However, polyploidy, aneuploidy and the high chromosome segregation in sugarcane which results various phenotypic characteristics variation and environmental effects become limiting factors to identify the progenies based on morphological characteristic. Microsatellite as one of molecular marker which has codominance inheritance, multiallelic, abundant in the genome and does not influenced by environmental factor is the best tool to asses the crossing fidelity accurately. This research aimed to identify the possibility of genetic marker of Saccharum spp. and Erianthus sp. on their hybrid using microsatellite molecular marker. This study was carried out in Molecular Genetic laboratory, Indonesian Sweetener and Fiber Crops Research Institute (ISFCRI) Malang, from August 2016 to July 2017. Eighty-six (86) F1 intraspecific and interspecific progeny, three commercial sugarcane varieties (PSJT941, PS881 and VMC7616) and two wild types (S. spontaneum dan Erianthus sp.) were assessed genetically by three microsatellite markers. Identification of microsatellite genetic markers was conducted by comparing the visualization band results from electrophoresis of each male and female parent through their progenies. All primers could identify Saccharum spp. and Erianthus sp. genetic markers. There were one to eleven Saccharum spp. and Erianthus sp. genetic markers could be identified such as 2-11 PS881-specific alleles; 2-3 VMC7616-specific alleles; 1-5 PSJT941-specific alleles; two S. spontaneum-specific alleles and 1-2 Erianthus-specific alleles. These findings could be used as the advance genetic marker of microsatellite in sugarcane breeding to asses the cross fidelity.

scholarly journals An Efficient PET-MRI Medical Image Fusion based on IHS-NSCT-PCA Integrated Method

2019 ◽  
Vol 9 (2) ◽  
pp. 2073-2079
Keyword(s):  
Structural Data ◽  
Fusion Rule ◽  
Integrated Approach ◽  
Disease Diagnosis ◽  
Weighted Averaging ◽  
Fusion Algorithm ◽  
Integrated Method ◽  
Histogram Matching ◽  
Multiple Imaging ◽  
Fused Image

Merging of multiple imaging modalities leads to a single image that acquire high information content. These find useful applications in disease diagnosis and treatment planning. IHS-PCA method is a spatial domain approach for fusion that offersfinestvisibility but demands vast memory and it lacks steering information. We propose an integrated approach that incorporates NSCT combined with PCA utilizing IHS space and histogram matching. The fusion algorithm is applied on MRI with PET image and improved functional property was obtained. The IHS transform is a sharpening technique that converts multispectral image from RGB channels to Intensity Hue and Saturation independent values. Histogram matching is performed with intensity values of the two input images. Pathological details in images can be emphasized in multi-scale and multi-directions by using PCA withNSCT. Fusion rule applied is weighted averaging andprincipal components are used for dimensionality reduction. Inverse NSCT and Inverse IHS are performed so as to obtain the fused image in new RGB space. Visual and subjective investigation is compared with existing methods which demonstrate that our proposed technique gives high structural data content with high spatial and spectral resolution compared withearlier methods.

scholarly journals Rare Renal Disease in Macedonia – An Update

PRILOZI ◽  
2017 ◽  
Vol 38 (3) ◽  
pp. 63-69
Author(s):  
Velibor Tasic ◽  
Zoran Gucev ◽  
Momir Polenakovic
Keyword(s):  
New England ◽  
Health Professionals ◽  
Molecular Genetic ◽  
Current Status ◽  
Renal Diseases ◽  
Treatment Interventions ◽  
New Genes ◽  
Appropriate Treatment ◽  
Genetic Techniques ◽  
Unites States

Abstract Rare renal diseases (RRD) are an important category of rare disease (RD) as they can do great damage to the patients, families and society. The patient may undergo years even decades of numerous investigations including invasive procedures and yet not have definitive and precise diagnose and therefore, no opportunity for appropriate treatment. The great progress in molecular genetic techniques characterized many Mendelian diseases on molecular level. This gave the possibility for appropriate prevention and treatment interventions, genetic counseling and prenatal diagnosis. Herein, we summarize the current status of RRD in Macedonia. The research interest of Macedonian clinicians and scientists is focused on the genetics of congenital anomalies of the kidney and urinary tract (CAKUT), steroid resistant nephrotic syndrome, nephrolithiasis and nephrocalcinosis, cystic diseases and cilliopathies with collaborations with eminent laboratories in Unites States and Europe. This collaboration resulted in detection of new genes and pathophysiological pathways published in The New England Journal of Medicine and in other high impact journals. Macedonian health professionals have knowledge and equipment for diagnosis of RRD. Unfortunately the lack of finances is great obstacle for early and appropriate diagnosis. Participation in the international registries, studies and trials should be encouraged. This would result in significant benefit for the patients, health professionals and science.

scholarly journals Molecular-genetic and phenotypic characteristics of desmoid-type fibromatosis

2019 ◽  
pp. 9-15
Author(s):  
T.A. Muzaffarova ◽  
O.V. Novikova ◽  
I.Yu. Sachkov ◽  
F.M. Kipkeeva ◽  
E.K. Ginter ◽  
...  
Keyword(s):  
Clinical Course ◽  
Age Of Onset ◽  
Molecular Genetic ◽  
Germline Mutations ◽  
Apc Gene ◽  
Phenotypic Characteristics ◽  
Potential Significance ◽  
Desmoid Fibromatosis ◽  
Apc Mutations ◽  
Sporadic Tumors

Desmoid-type fibromatosis (DF) is a rare mesenchymal tumor occurring in only 2 to 4 people per 1,000,000 population a year. Desmoid tumors are either seen sporadically or in individuals with familial adenomatous polyposis (FAP). The etiology of sporadic DF is uncertain. The aim of this study was to estimate the potential significance of germline mutations in the APC gene in patients with sporadic DF. APC exons were amplified, studied using conformation sensitive gel electrophoresis and then Sanger-sequenced. The obtained data were processed in Statistica 10. Mutations were detected in 6 (12%) of 51 participants with sporadic DF. Those 6 patients shared a typical DF phenotype characterized by early age of onset (5.8 years on average, in contrast to the patients without APC mutations, who developed DF at 19 years of age; p = 0.02), severe clinical course, multifocal localization on the trunk, and poor prognosis. All of the detected APC mutations were localized to the 3'-end of the gene. For the purpose of comparison, we analyzed a sample of 12 patients with FAP-associated DF. Of those patients, 6 carried mutations in the APC gene. In the analyzed sample, the patients with FAP and the mutant APC gene developed DF at older age (35 years) than the patients with sporadic DF (p = 0.004) and their tumors were not multifocal. This means that sporadic and FAP-associated desmoids have different phenotypes in patients with APC mutations. Patients with sporadic tumors have mutations at the 3'-end of the APC gene more often than individuals with FAP-associated DF. To our knowledge, this is the first study to characterize the subtype of sporadic desmoid fibromatosis phenotypically determined by germline mutations in the APC gene.

scholarly journals Integrated method for the measurement of trace nitrogenous atmospheric bases

2011 ◽  
Vol 4 (12) ◽  
pp. 2795-2807 ◽  
Author(s):  
D. Key ◽  
J. Stihle ◽  
J.-E. Petit ◽  
C. Bonnet ◽  
L. Depernon ◽  
...  
Keyword(s):  
Nitrogen Cycle ◽  
Field Measurements ◽  
Ambient Air ◽  
Integrated Approach ◽  
Urban Park ◽  
Sampling Strategies ◽  
Road Vehicles ◽  
Integrated Method ◽  
The Many ◽  
Global Nitrogen Cycle

Abstract. Nitrogenous atmospheric bases are thought to play a key role in the global nitrogen cycle, but their sources, transport, and sinks remain poorly understood. Of the many methods available to measure such compounds in ambient air, few meet the current need of being applicable to the complete range of potential analytes and fewer still are convenient to implement using instrumentation that is standard to most laboratories. In this work, an integrated approach to measuring trace, atmospheric, gaseous nitrogenous bases has been developed and validated. The method uses a simple acid scrubbing step to capture and concentrate the bases as their phosphite salts, which then are derivatized and analyzed using GC/MS and/or LC/MS. The advantages of both techniques in the context of the present measurements are discussed. The approach is sensitive, selective, reproducible, as well as convenient to implement and has been validated for different sampling strategies. The limits of detection for the families of tested compounds are suitable for ambient measurement applications (e.g., methylamine, 1 pptv; ethylamine, 2 pptv; morpholine, 1 pptv; aniline, 1 pptv; hydrazine, 0.1 pptv; methylhydrazine, 2 pptv), as supported by field measurements in an urban park and in the exhaust of on-road vehicles.

Molecular Genetic Techniques for Algal Bioengineering

2015 ◽  
pp. 155-171 ◽  
Author(s):  
Kenan Jijakli ◽  
Rasha Abdrabu ◽  
Basel Khraiwesh ◽  
David R. Nelson ◽  
Joseph Koussa ◽  
...  
Keyword(s):  
Molecular Genetic ◽  
Genetic Techniques

Behavior and Molecular Genetics of the Five Factor Model

2015 ◽  
Author(s):  
Amber M. Jarnecke ◽  
Susan C. South
Keyword(s):  
Molecular Genetics ◽  
Behavior Genetics ◽  
Factor Model ◽  
Five Factor Model ◽  
Molecular Genetic ◽  
Future Directions ◽  
Genetics Research ◽  
Genetic Mechanisms ◽  
Genetic Techniques ◽  
Related Personality

Behavior and molecular genetics informs knowledge of the etiology, structure, and development of the Five Factor Model (FFM) of personality. Behavior genetics uses quantitative modeling to parse the relative influence of nature and nurture on phenotypes that vary within the population. Behavior genetics research on the FFM has demonstrated that each domain has a heritability (proportion of variation due to genetic influences) of 40–50%. Molecular genetic methods attempt to identify specific genetic mechanisms associated with personality variation. To date, findings from molecular genetics are tentative, with significant results failing to replicate and accounting for only a small percentage of the variance. However, newer techniques hold promise for finding the “missing heritability” of FFM and related personality domains. This chapter presents an overview of commonly used behavior and molecular genetic techniques, reviews the work that has been done on the FFM domains and facets, and offers a perspective for future directions.

Sign in / Sign up

Export Citation Format

Share Document