scholarly journals Microsatellite Genetic Markers of Saccharrum spp., and Erianthus sp. on Their Hybrids

2019 ◽  
Vol 3 (1) ◽  
pp. 1
Author(s):  
Mala Murianingrum ◽  
Taryono Taryono ◽  
Rani Agustina Wulandari
Keyword(s):  
Molecular Marker ◽  
Genetic Marker ◽  
Genetic Markers ◽  
Chromosome Segregation ◽  
Molecular Genetic ◽  
Female Parent ◽  
Limiting Factors ◽  
Phenotypic Characteristics ◽  
Saccharum Spp ◽  
Sugarcane Varieties

Progeny identification is the important step that should be done after hybridization. However, polyploidy, aneuploidy and the high chromosome segregation in sugarcane which results various phenotypic characteristics variation and environmental effects become limiting factors to identify the progenies based on morphological characteristic. Microsatellite as one of molecular marker which has codominance inheritance, multiallelic, abundant in the genome and does not influenced by environmental factor is the best tool to asses the crossing fidelity accurately. This research aimed to identify the possibility of genetic marker of Saccharum spp. and Erianthus sp. on their hybrid using microsatellite molecular marker. This study was carried out in Molecular Genetic laboratory, Indonesian Sweetener and Fiber Crops Research Institute (ISFCRI) Malang, from August 2016 to July 2017. Eighty-six (86) F1 intraspecific and interspecific progeny, three commercial sugarcane varieties (PSJT941, PS881 and VMC7616) and two wild types (S. spontaneum dan Erianthus sp.) were assessed genetically by three microsatellite markers. Identification of microsatellite genetic markers was conducted by comparing the visualization band results from electrophoresis of each male and female parent through their progenies. All primers could identify Saccharum spp. and Erianthus sp. genetic markers. There were one to eleven Saccharum spp. and Erianthus sp. genetic markers could be identified such as 2-11 PS881-specific alleles; 2-3 VMC7616-specific alleles; 1-5 PSJT941-specific alleles; two S. spontaneum-specific alleles and 1-2 Erianthus-specific alleles. These findings could be used as the advance genetic marker of microsatellite in sugarcane breeding to asses the cross fidelity.

Molecular genetic markers for thyroid FNAB

2015 ◽  
Vol 54 (03) ◽  
pp. 94-100 ◽  
Author(s):  
P. B. Musholt ◽  
T. J. Musholt
Keyword(s):  
Genetic Markers ◽  
Thyroid Nodules ◽  
Molecular Genetic ◽  
Genetic Alterations ◽  
Diagnostic Tools ◽  
Ultrasound Screening ◽  
Thyroid Malignancy ◽  
Thyroid Carcinomas ◽  
Molecular Genetic Markers ◽  
The Impact

SummaryAim: Thyroid nodules > 1 cm are observed in about 12% of unselected adult employees aged 18–65 years screened by ultrasound scan (40). While intensive ultrasound screening leads to early detection of thyroid diseases, the determination of benign or malignant behaviour remains uncertain and may trigger anxieties in many patients and their physicians. A considerable number of thyroid resections are consecutively performed due to suspicion of malignancy in the detected nodes. Fine needle aspiration biopsy (FNAB) has been recommended for the assessment of thyroid nodules to facilitate detection of thyroid carcinomas but also to rule out malignancy and thereby avoid unnecessary thyroid resections. However, cytology results are dependent on experience of the respective cytologist and unfortunately inconclusive in many cases. Methods: Molecular genetic markers are already used nowadays to enhance sensitivity and specificity of FNAB cytology in some centers in Germany. The most clinically relevant molecular genetic markers as pre-operative diagnostic tools and the clinical implications for the intraoperative and postoperative management were reviewed. Results: Molecular genetic markers predominantly focus on the preoperative detection of thyroid malignancies rather than the exclusion of thyroid carcinomas. While some centers routinely assess FNABs, other centers concentrate on FNABs with cytology results of follicular neoplasia or suspicion of thyroid carcinoma. Predominantly mutations of BRAF, RET/PTC, RAS, and PAX8/PPARγ or expression of miRNAs are analyzed. However, only the detection of BRAF mutations predicts the presence of (papillary) thyroid malignancy with almost 98% probability, indicating necessity of oncologic thyroid resections irrespective of the cytology result. Other genetic alterations are associated with thyroid malignancy with varying frequency and achieve less impact on the clinical management. Conclusion: Molecular genetic analysis of FNABs is increasingly performed in Germany. Standardization, quality controls, and validation of various methods need to be implemented in the near future to be able to compare the results. With increasing knowledge about the impact of genetic alterations on the prognosis of thyroid carcinomas, recommendations have to be defined that may lead to individually optimized treatment strategies.

Molecular genetic markers for assessing individual sensitivity to lead

2019 ◽  
Author(s):  
Л.П. Кузьмина ◽  
◽  
А.Г. Хотулева ◽  
М.М. Коляскина ◽  
Л.М. Безрукавникова ◽  
...  
Keyword(s):  
Genetic Markers ◽  
Molecular Genetic ◽  
Individual Sensitivity ◽  
Molecular Genetic Markers

Breeding study of sea buckthorn (Hippophae rhamnoides L.) in the Institute of Horticulture, NAAS оf Ukraine

2020 ◽  
pp. 37-49
Author(s):  
V.V. Moskalets ◽  
◽  
T.Z. Moskalets ◽  
I.V. Grynyk ◽  
O.A. Shevchuk ◽  
...  
Keyword(s):  
Genetic Markers ◽  
Cell Biology ◽  
Molecular Genetic ◽  
The State ◽  
Sea Buckthorn ◽  
National Academy Of Sciences ◽  
Scientific Cooperation ◽  
State Register ◽  
Academy Of Sciences

The authors present the results of the sea buckthorn breeding at the Institute of Horticulture (NAAS). The stages of the work have been analyzed – from studying and selecting the initial material in the conditions of the Polissya, Polissya-Lisosteppe and Lisosteppe ecotopes (2012-2016) to the successful targeted introduction to the Northern part of the Lisosteppe (2017-2019) and new forms have been characterized according to the traits valuable for economy and molec-ular genetic markers. The new forms of the researched crop taking into consideration the high indices of their productiv-ity,adaptivity to the unfavourable abiotic and biotic environmental factors and consumption quality of fruits for pro-cessing and making functionary products were entered officially into Genetic Fund of the Plants of Ukraine as con-firmed by the certificates of copyright and developed genetic passports. The list of these genotypes includes 1-15-1 (Nos-ivchanka, UA3700073), 1-15-8S (Mitsna, UA3700079), make form 1-15-6Ch (Aboryhen 6/11, UA3700080), 1-15-9 Ka-rotynna, UA3700082), 1-15-3 (Pamiatka, UA3700076), 1-15-8V (Soniachne siayvo, UA3700075), 1-15-11 (Lymonna, UA3700072), 2-15-73 (Morkviana, UA3700077), 1-15-5 (Adaptyvna, UA3700078), 1-15-8B (Osoblyva, UA3700083), 1-15-6 (Apelsynova, UA3700084) and forms 6A/11 (UA3700081), 1-15-5a (Sribnolysta 5a, UA3700074). The possibility of using 5 DNA markers to characterize genotypes of sea buckthorn bymeans of the molecular genetic markers was tested and evaluated in the framework of the scientific cooperation with the Institute of Cell Biology and Genetic Engineering of the National Academy of Sciences of Ukraine. It should be noted that the most polymorphic markers were HrMS025 and HrMS026. However, the marker HrMS014 was monomorphic, but appeared in all the samples, so it can be used as a reference. The best forms of sea buckthorn Adaptyvna (certificate №190899) and Osoblyva (certificate №190900) were included into the State Register of Plant Varieties Suitable for dissemination in Ukraine, and the cultivars of the univer-sal use Nadiina (applications №18299010), Oliana (applications №18299009) and Morkviana (applications № 20299001) and cv pollinator Obrii (applications №18299008) undergo the State strain test. The attention is concentrat-ed on the promising directions of the new sea buckthorn genotypes for the prior breeding and genetic investigations at the Institute of Horticulture (NAAS) and its network.

Molecular genetic markers in diagnosis of lung cancer

2011 ◽  
Vol 45 (2) ◽  
pp. 175-189 ◽  
Author(s):  
A. A. Ponomaryova ◽  
E. Yu. Rykova ◽  
N. V. Cherdyntseva ◽  
E. L. Choinzonov ◽  
P. P. Laktionov ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Genetic Markers ◽  
Molecular Genetic ◽  
Molecular Genetic Markers ◽  
Diagnosis Of Lung Cancer

The Leucocyte Antigenic System HL-A as a Possible Genetic Marker of Schizophrenia

1974 ◽  
Vol 125 (584) ◽  
pp. 25-27 ◽  
Author(s):  
C. L. Cazzullo ◽  
E. Smeraldi ◽  
G. Penati
Keyword(s):  
Environmental Factors ◽  
Genetic Marker ◽  
Genetic Markers ◽  
Genetic Predisposition ◽  
Age At Onset ◽  
Familial Occurrence ◽  
The Real ◽  
Genetically Determined

Many inheritance models of schizophrenia have been proposed, in view of its variable age at onset, variable familial occurrence and the relevant influence of a great number of environmental factors (Gottsman and Shields, 1967; Heston, 1970; Ödegård, 1972). However, the real genetic predisposition to schizophrenia has not yet been experimentally verified, and genetic markers have to be looked for, i.e. we need some character, genetically determined, whose transmission is associated with schizophrenia transmission: the more polymorphous the character, the greater will be the probability of finding such associations.

Molecular genetic markers of intra- and interspecific divergence within starfish and sea urchins (Echinodermata)

2016 ◽  
Vol 81 (9) ◽  
pp. 972-980 ◽  
Author(s):  
N. B. Petrov ◽  
I. P. Vladychenskaya ◽  
A. L. Drozdov ◽  
O. S. Kedrova
Keyword(s):  
Genetic Markers ◽  
Sea Urchins ◽  
Molecular Genetic ◽  
Molecular Genetic Markers ◽  
Interspecific Divergence

DIFFERENTIAL EFFECT OF SALINITY ON GROWTH, NUTRIENT CONCENTRATION AND CHLOROPHYLL IN SUGARCANE VARIETIES MEX 69-290 AND CP 72-2086

Agrociencia ◽  
2021 ◽  
Vol 55 (7) ◽  
pp. 597-610
Author(s):  
Odón Castañeda Castro ◽  
Miriam Cristina Pastelín Solano ◽  
Libia Iris Trejo Téllez ◽  
Eduardo Ariel Solano Pastelín ◽  
Fernando Carlos Gómez Merino
Keyword(s):  
Plant Height ◽  
Plant Performance ◽  
Saccharum Spp ◽  
Sugarcane Varieties ◽  
Randomized Design ◽  
Affected Plant ◽  
Nacl Concentration ◽  
Irrigation Solution ◽  
Completely Randomized Design ◽  
Better Than

Sugarcane (Saccharum spp. hybrids) is moderately sensitive to salinity and the effects on plant performance vary according to stress level and genotype. This study aimed to evaluate the effect of salt stress induced by application of different NaCl levels in the irrigation solution on plant height, indirect index of chlorophylls (SPAD), and macronutrients concentration (N, P, K, Ca, Mg and S) and Na in leaves of two sugarcane varieties: CP 72-2086 and Mex 69-290. The experiment was set in a completely randomized design with a 2×5 factorial arrangement. The study factors were sugarcane variety (CP 72-2086 and Mex 69-290) and NaCl concentration (0.0, 71.8, 143.6, 215.4 and  282.7 mM NaCl). Salinity as a single factor negatively affected plant height, SPAD units and N and P concentration in leaves; Ca concentrations increased, while K, Mg and S remained unaffected by the tested NaCl levels. Mex 69-290 grew higher and concentrated greater levels of N and K. Interactions of factors showed that salinity reduced growth in both varieties, but this reduction was more pronounced in CP 72-2086. SPAD units were also significantly reduced by salinity in both varieties. Concentrations of N and P in leaves decreased in both varieties in response to NaCl, while those of K and Ca increased in Mex 69-290. Concentration of Na was higher in Mex 69-290 which exhibited better performance than CP 72-2086. Sodium concentrations in leaves increased in direct relation to the tested NaCl concentrations. Mex 69-290 reached higher concentrations of Na in leave tissues but displayed better health than CP 72-2086. Thus, the variety Mex 69-290 showed more efficient Na-tolerance mechanisms related to Ca and K concentrations, and an indirect chlorophyll index better than CP 72-2086.

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