scholarly journals Molecular-genetic marking of Brassica L. species for resistance against various pathogens: achievements and prospects

2019 ◽  
Vol 23 (6) ◽  
pp. 656-666 ◽  
Author(s):  
F. A. Berensen ◽  
O. Yu. Antonova ◽  
А. M. Artemyeva
Keyword(s):  
Molecular Genetic ◽  
Genetic Marking

Применение метода молекулярно-генетического анализа для выявления растений моркови с цитоплазмой типа «петалоид»

2018 ◽  
Author(s):  
A.V. Chistova
Keyword(s):  
Material Selection ◽  
Molecular Genetic ◽  
Practical Importance ◽  
F1 Hybrids ◽  
Breeding Station ◽  
Pcr Products ◽  
Selection For ◽  
Genetic Marking ◽  
Sterility Genes

Метод молекулярно-генетического маркирования позволяет проводить скрининг коллекции исходного материала по типу цитоплазмы. Дифференциация растений моркови по типу цитоплазмы имеет большое практическое значение при выборе растительного материала для создания новых линий-закрепителей стерильности. Мужская стерильность моркови контролируется ядерно-цитоплазматически, и растения с «петалоидной» цитоплазмой могут иметь фертильный фенотип за счет генов ядра. Однако попытка получить закрепитель стерильности из такого растения обречена на неудачу, при том что будет потрачено много времени и труда. В связи с этим целесообразно на ранних этапах селекции проводить молекулярно-генетический анализ коллекции растений с помощью рекомендованных в научной литературе праймеров, и отобрать образцы с нормальной цитоплазмой. Для этого использовали праймеры, разработанные Inga C. Bach c соавторами (2002). Эффективным оказалось применение трех пар праймеров сmt-1 и cmt-2 (длины амплифицированных фрагментов 320 и 390 п. н.), atp1-d1 и cmt-3 (размер ПЦР продуктов 1630 п. н.), atp1-d1 и cmt-4 (1608 п. н.). С их помощью был проведен скрининг коллекции моркови ООО «Селекционная станция им. Н.Н.Тимофеева» и отобраны растения для дальнейшей селекционной работы. Среди изученных F1 гибридов были выявлены стерильные растения с цитоплазмой типа «петалоид», фертильные растения с петалоидной цитоплазмой и фертильные растения с нормальной цитоплазмой. Чрезвычайно полезной была бы возможность с помощью молекулярно-генетического маркирования определять наличие ядерных генов стерильности моркови и гомозиготность. Однако при проведении данной работы применение соответствующих праймеров было не результативно. Так что традиционные методы селекции для решения этой задачи остаются незаменимыми.The method of molecular genetic marking can be used to differentiate carrot plants by the type of cytoplasm. It has a great practical importance for the plant material selection for new sterility fixers creation. Carrots male sterility has a nuclear-cytoplasmic control, plants with a «petaloid» cytoplasm can be fertile due to nuclear genes. But the attempt of using such plants to obtain a fixer of sterility is doomed to failure, and a lot of time and labor will be lost. One can select plants with normal cytoplasm at the early stages of breeding by the molecular-genetic primers using. To achieve this aim, primers developed by Inga C. Bach et al. (2002) were applied. The application of three pairs of primers was effective. These primers are: 1) cmt-1 and cmt-2 (lengths of amplified fragments 320 and 390 bp), 2) atp1-d1 and cmt-3 (size of PCR products 1630 bp), 3) atp1-d1 and cmt-4 (1608 bp). Through the application of these primers, the collection of carrots of Breeding station after N.N. Timofeev was screened. The application of this method allowed to select plants for subsequent breeding process. Carrot plants of F1-hybrids were examined. Among them were found:1) sterile plants with a cytoplasm of the «petaloid» type, 2) fertile plants with a «petaloid» cytoplasm and 3) fertile plants with a normal cytoplasm. It would be incredibly useful to determine the presence of nuclear carrot sterility genes and homozygosity by the instrumentality of molecular-genetic marking. However, traditional methods of plant breeding remain indispensable. Howbeit. carrot plants with petaloid or normal cytoplasm can be detected by primers using.

Molecular genetic markers for thyroid FNAB

2015 ◽  
Vol 54 (03) ◽  
pp. 94-100 ◽  
Author(s):  
P. B. Musholt ◽  
T. J. Musholt
Keyword(s):  
Genetic Markers ◽  
Thyroid Nodules ◽  
Molecular Genetic ◽  
Genetic Alterations ◽  
Diagnostic Tools ◽  
Ultrasound Screening ◽  
Thyroid Malignancy ◽  
Thyroid Carcinomas ◽  
Molecular Genetic Markers ◽  
The Impact

SummaryAim: Thyroid nodules > 1 cm are observed in about 12% of unselected adult employees aged 18–65 years screened by ultrasound scan (40). While intensive ultrasound screening leads to early detection of thyroid diseases, the determination of benign or malignant behaviour remains uncertain and may trigger anxieties in many patients and their physicians. A considerable number of thyroid resections are consecutively performed due to suspicion of malignancy in the detected nodes. Fine needle aspiration biopsy (FNAB) has been recommended for the assessment of thyroid nodules to facilitate detection of thyroid carcinomas but also to rule out malignancy and thereby avoid unnecessary thyroid resections. However, cytology results are dependent on experience of the respective cytologist and unfortunately inconclusive in many cases. Methods: Molecular genetic markers are already used nowadays to enhance sensitivity and specificity of FNAB cytology in some centers in Germany. The most clinically relevant molecular genetic markers as pre-operative diagnostic tools and the clinical implications for the intraoperative and postoperative management were reviewed. Results: Molecular genetic markers predominantly focus on the preoperative detection of thyroid malignancies rather than the exclusion of thyroid carcinomas. While some centers routinely assess FNABs, other centers concentrate on FNABs with cytology results of follicular neoplasia or suspicion of thyroid carcinoma. Predominantly mutations of BRAF, RET/PTC, RAS, and PAX8/PPARγ or expression of miRNAs are analyzed. However, only the detection of BRAF mutations predicts the presence of (papillary) thyroid malignancy with almost 98% probability, indicating necessity of oncologic thyroid resections irrespective of the cytology result. Other genetic alterations are associated with thyroid malignancy with varying frequency and achieve less impact on the clinical management. Conclusion: Molecular genetic analysis of FNABs is increasingly performed in Germany. Standardization, quality controls, and validation of various methods need to be implemented in the near future to be able to compare the results. With increasing knowledge about the impact of genetic alterations on the prognosis of thyroid carcinomas, recommendations have to be defined that may lead to individually optimized treatment strategies.

Molecular genetic background of haemophilia A patients with discrepancy between one-stage and two-stage factor VIII assays

2010 ◽  
Vol 30 (S 01) ◽  
pp. S153-S155
Author(s):  
D. Delev ◽  
S. Pahl ◽  
J. Driesen ◽  
H. Brondke ◽  
J. Oldenburg ◽  
...  
Keyword(s):  
Factor Viii ◽  
Genetic Background ◽  
Molecular Genetic ◽  
Haemophilia A ◽  
Two Stage ◽  
One Stage

Abnormal Processing of the Glycoprotein IIb Transcript due to a Nonsense Mutation in Exon 17 Associated with Glanzmann’s Thrombasthenia

1995 ◽  
Vol 73 (05) ◽  
pp. 756-762 ◽  
Author(s):  
Yoshiaki Tomiyama ◽  
Hirokazu Kashiwagi ◽  
Satoru Kosugi ◽  
Masamichi Shiraga ◽  
Yoshio Kanayama ◽  
...  
Keyword(s):  
Dna Analysis ◽  
Molecular Genetic ◽  
Genetic Defect ◽  
Nucleotide Sequence Analysis ◽  
Type I ◽  
Normal Amount ◽  
Immunoblot Assay ◽  
Glanzmann’S Thrombasthenia ◽  
Glanzmann's Thrombasthenia ◽  
Pcr Products

SummaryWe analyzed the molecular genetic defect responsible for type I Glanzmann’s thrombasthenia in a Japanese patient. In an immunoblot assay using polyclonal anti-GPIIb-IIIa antibodies, some GPIIIa (15% of normal amount) could be detected in the patient’s platelets, whereas GPIIb could not (<2% of normal amount). Nucleotide sequence analysis of platelet GPIIb mRNA-derived polymerase chain reaction (PCR) products revealed that patient’s GPIIb cDNA had a 75-bp deletion in the 3’ boundary of exon 17 resulting in an in-frame deletion of 25 amino acids. DNA analysis and family study revealed that the patient was a compound heterozygote of two GPIIb gene defects. One allele derived from her father was not expressed in platelets, and the other allele derived from her mother had a 9644C → T mutation which was located at the position -3 of the splice donor junction of exon 17 and resulted in a termination codon (TGA). Moreover, quantitative analysis demonstrated that the amount of the abnormal GPIIb transcript in the patient’s platelets was markedly reduced. Thus, the C → T mutation resulting in the abnormal splicing of GPIIb transcript and the reduction in its amount is responsible for Glanzmann’s thrombasthenia.

HLA Genotype of Patients with Severe Haemophilia A due to Intron 22 Inversion with and without Inhibitors of Factor VIII

1997 ◽  
Vol 77 (02) ◽  
pp. 238-242 ◽  
Author(s):  
J Oldenburg ◽  
J K Picard ◽  
R Schwaab ◽  
H H Brackmann ◽  
E G D Tuddenham ◽  
...  
Keyword(s):  
Factor Viii ◽  
Major Gene ◽  
Statistical Significance ◽  
Strong Association ◽  
Molecular Genetic ◽  
Single Mutation ◽  
Severe Haemophilia ◽  
Extended Haplotype ◽  
Intron 22 Inversion ◽  
Hla Genotype

SummaryMolecular genetic studies have shown that development of antibodies to factor VIII (inhibitors) occurs most frequently in patients with severe haemophilia due to major gene lesions including inversions, stop codons and large deletions. Previous studies of HLA type were performed on inhibitor and non-inhibitor patients with diverse uncharacterised mutations which may have confounded detection of significant associations. We therefore selected a group of patients with a single mutation type, the prevalent intron 22 inversion, with or without inhibitors, to determine HLA genotype. Seventy-one such patients, 42 without and 29 with inhibitors (13 high, 9 low and 7 transient responders) were genotyped for MHC Class I HLA-A, -B, -C and Class II HLA-DQA, -DQB and -DRB loci. No strong correlation of any HLA-allele to inhibitor or non-inhibitor status was found. However, alleles of the haplotype HLA-A3, HLA-B7, HLA-C7, HLA-DQA0102, HLA-DQB0602, HLA-DR15 occurred more often in inhibitor patients. Since the alleles of this extended haplotype are common in the North European population only a very strong association would achieve statistical significance. Further studies of groups of patients similar to those studied here will be needed to confirm or exclude this association.

Molecular Basis and Clinical Aspects of Hereditary Megakaryocyte and Platelet Membrane Glycoprotein Disorders

1996 ◽  
Vol 16 (02) ◽  
pp. 114-138 ◽  
Author(s):  
R. E. Scharf
Keyword(s):  
Genetic Basis ◽  
Molecular Mechanisms ◽  
Molecular Genetic ◽  
Platelet Membrane ◽  
Clinical Aspects ◽  
Membrane Glycoprotein ◽  
Membrane Glycoproteins ◽  
Membrane Expression ◽  
Receptor Complexes ◽  
Platelet Membrane Glycoprotein

SummarySpecific membrane glycoproteins (GP) expressed by the megakaryocyte-platelet system, including GPIa-lla, GPIb-V-IX, GPIIb-llla, and GPIV are involved in mediat-ing platelet adhesion to the subendothelial matrix. Among these glycoproteins, GPIIb-llla plays a pivotal role since platelet aggregation is exclusively mediated by this receptor and its interaction with soluble macromolecular proteins. Inherited defects of the GPIIb-llla or GPIb-V-IX receptor complexes are associated with bleeding disorders, known as Glanzmann's thrombasthenia, Bernard-Soulier syndrome, or platelet-type von Willebrand's disease, respectively. Using immuno-chemical and molecular biology techniques, rapid advances in our understanding of the molecular genetic basis of these disorders have been made during the last few years. Moreover, analyses of patients with congenital platelet membrane glycoprotein abnormalities have provided valuable insights into molecular mechanisms that are required for structural and functional integrity, normal biosynthesis of the glycoprotein complexes and coordinated membrane expression of their constituents. The present article reviews the current state of knowledge of the major membrane glycoproteins in health and disease. The spectrum of clinical bleeding manifestations and established diagnostic criteria for each of these dis-orders are summarized. In particular, the variety of molecular defects that have been identified so far and their genetic basis will be discussed.

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