scholarly journals PDZ-binding kinase/T-LAK cell-originated protein kinase is a target of the fucoidan from brown alga Fucus evanescens in the prevention of EGF-induced neoplastic cell transformation and colon cancer growth

Oncotarget ◽  
2016 ◽  
Vol 7 (14) ◽  
pp. 18763-18773 ◽  
Author(s):  
Olesia S. Vishchuk ◽  
Huimin Sun ◽  
Zhe Wang ◽  
Svetlana P. Ermakova ◽  
JuanJuan Xiao ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Protein Kinase ◽  
Brown Alga ◽  
Cell Transformation ◽  
Neoplastic Cell ◽  
Cancer Growth ◽  
Fucus Evanescens ◽  
Lak Cell ◽  
Neoplastic Cell Transformation

scholarly journals Impaired Expression of Cytoplasmic Actins Leads to Chromosomal Instability of MDA-MB-231 Basal-Like Mammary Gland Cancer Cell Line

Molecules ◽  
2021 ◽  
Vol 26 (8) ◽  
pp. 2151
Author(s):  
Vera Dugina ◽  
Galina Shagieva ◽  
Mariya Novikova ◽  
Svetlana Lavrushkina ◽  
Olga Sokova ◽  
...  
Keyword(s):  
Cell Line ◽  
Chromosomal Instability ◽  
Cell Transformation ◽  
Cancer Cell Line ◽  
Neoplastic Cell ◽  
Chromosome Morphology ◽  
Human Carcinoma ◽  
Actin Isoforms ◽  
Cytoplasmic Actin ◽  
Neoplastic Cell Transformation

We have shown previously that two cytoplasmic actin isoforms play different roles in neoplastic cell transformation. Namely, β-cytoplasmic actin acts as a tumor suppressor, whereas γ-cytoplasmic actin enhances malignant features of tumor cells. The distinct participation of each cytoplasmic actin in the cell cycle driving was also observed. The goal of this study was to describe the diverse roles of cytoplasmic actins in the progression of chromosomal instability of MDA-MB-231 basal-like human carcinoma cell line. We performed traditional methods of chromosome visualization, as well as 3D-IF microscopy and western blotting for CENP-A detection/quantification, to investigate chromosome morphology. Downregulation of cytoplasmic actin isoforms alters the phenotype and karyotype of MDA-MB-231 breast cancer cells. Moreover, β-actin depletion leads to the progression of chromosomal instability with endoreduplication and aneuploidy increase. On the contrary, γ-actin downregulation results not only in reduced percentage of mitotic carcinoma cells, but leads to chromosome stability, reduced polyploidy, and aneuploidy.

scholarly journals Baicalin suppresses lung cancer growth by targeting PDZ-binding kinase/T-LAK cell-originated protein kinase

2019 ◽  
Vol 39 (4) ◽  
Author(s):  
Xin Diao ◽  
Danfen Yang ◽  
Yu Chen ◽  
Wentian Liu
Keyword(s):  
Lung Cancer ◽  
Protein Kinase ◽  
Cancer Cells ◽  
Ex Vivo ◽  
Lung Cancer Cells ◽  
Cancer Growth ◽  
Downstream Signaling ◽  
Lak Cell

AbstractBaicalin is the main bioactive component extracted from the traditional Chinese medicine Baical Skullcap Root, and its anti-tumor activity has been studied in previous studies. PDZ-binding kinase/T-LAK cell-originated protein kinase (PBK/TOPK), a serine/threonine protein kinase, is highly expressed in many cancer cells and stimulates the tumorigenic properties, and so, it is a pivotal target for agent to cure cancers. We reported for the first time that baicalin suppressed PBK/TOPK activities by directly binding with PBK/TOPK in vitro and in vivo. Ex vivo studies showed that baicalin suppressed PBK/TOPK activity in JB6 Cl41 cells and H441 lung cancer cells. Moreover, knockdown of PBK/TOPK in H441 cells decreased their sensitivity to baicalin. In vivo study indicated that injection of baicalin in H441 tumor-bearing mice effectively suppressed cancer growth. The PBK/TOPK downstream signaling molecules Histone H3 and ERK2 in tumor tissues were also decreased after baicalin treatment. Taken together, baicalin can inhibit proliferation of lung cancer cells as a PBK/TOPK inhibitor both in vitro and in vivo.

scholarly journals Myricetin is a novel natural inhibitor of neoplastic cell transformation and MEK1

2007 ◽  
Vol 28 (9) ◽  
pp. 1918-1927 ◽  
Author(s):  
K. W. Lee ◽  
N. J. Kang ◽  
E. A. Rogozin ◽  
H.-G. Kim ◽  
Y. Y. Cho ◽  
...  
Keyword(s):  
Cell Transformation ◽  
Neoplastic Cell ◽  
Neoplastic Cell Transformation ◽  
Natural Inhibitor

Neoplastic Cell Transformation by Heavy Ions

1989 ◽  
Vol 120 (3) ◽  
pp. 468 ◽  
Author(s):  
Masao Suzuki ◽  
Masami Watanabe ◽  
Keiji Suzuki ◽  
Kazushiro Nakano ◽  
Ichiro Kaneko
Keyword(s):  
Heavy Ions ◽  
Cell Transformation ◽  
Neoplastic Cell ◽  
Neoplastic Cell Transformation
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