scholarly journals Cell activity during peripheral nerve defect repair process using a nerve scaffold

Oncotarget ◽  
2017 ◽  
Vol 8 (69) ◽  
pp. 113828-113836
Author(s):  
Chan Zhou ◽  
Jin Li ◽  
Huajian You ◽  
Jinfeng Lv ◽  
Jinlong Yang ◽  
...  
Keyword(s):  
Peripheral Nerve ◽  
Cell Activity ◽  
Repair Process ◽  
Defect Repair

scholarly journals Histopathological analysis of gangliosides use in peripheral nerve regeneration after axonotmesis in rats

2008 ◽  
Vol 23 (4) ◽  
pp. 364-371 ◽  
Author(s):  
Camila Maria Beder Ribeiro ◽  
Belmiro Cavalcanti do Egito Vasconcelos ◽  
Joaquim Celestino da Silva Neto ◽  
Valdemiro Amaro da Silva Júnior ◽  
Nancy Gurgel Figueiredo
Keyword(s):  
Sciatic Nerve ◽  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Schwann Cells ◽  
Peripheral Nerve Regeneration ◽  
Cell Activity ◽  
Control Group ◽  
Histopathological Analysis ◽  
Male Wistar Rats ◽  
The Right

PURPOSE: To analyze the action of gangliosides in peripheral nerve regeneration in the sciatic nerve of the rat. METHODS: The sample was composed of 96 male Wistar rats. The animals were anaesthetized and, after identification of the anaesthesic plane, an incision was made in the posterior region of the thigh, followed by skin and muscle divulsion. The right sciatic nerve was isolated and compressed for 2 minutes. Continuous suture of the skin was performed. The animals were randomly divided into two groups: the experimental group (EG), which received subcutaneous injection of gangliosides, and the control group (CG), which received saline solution (0.9%) to mimic the effects of drug administration. RESULTS: No differences were observed between the experimental and control groups evaluated on the eighth day of observation. At 15 and 30 days the EG showed an decrease in Schwann cell activity and an apparent improvement in fibre organization; at 60 days, there was a slight presence of Schwann cells in the endoneural space and the fibres were organized, indicating nerve regeneration. At 15 and 30 days, the level of cell reaction in the CG had diminished, but there were many cells with cytoplasm in activity and in mitosis; at 60 days, hyperplastic Schwann cells and mitotic activity were again observed, as well as nerve regeneration, but to a lesser extent than in the EG. CONCLUSION: The administration of exogenous gangliosides seems to improve nerve regeneration.

Characterization of Biomaterial and Tissue Response Analysis after Implantation

2018 ◽  
Vol 930 ◽  
pp. 48-52
Author(s):  
Eliana dos Santos Câmara-Pereira ◽  
Ana Emília Holanda Rolim ◽  
Isabela Cerqueira Barreto ◽  
Laise Monteiro Campos Moraes ◽  
Lilian Campos ◽  
...  
Keyword(s):  
Repair Process ◽  
Tissue Response ◽  
Precipitation Method ◽  
Molar Ratio ◽  
Osteogenic Potential ◽  
Response Analysis ◽  
Defect Site ◽  
Critical Defect ◽  
Defect Repair

Some biomaterials can be used to promote tissue repair process. The biological substitutes (biomaterials such as hydroxyapatite beads) can be used with some advantages and purpose of mimicking responses to on-site repair of the injured bone. The objective of this study was to evaluate the osteogenic potential of the biomaterial composed of hydroxyapatite and alginate in place of the critical defect. bioceramic samples stoichiometric hydroxyapatite was produced by the precipitation method, wet method with ion molar ratio of Ca 10 (PO 4) 6 (OH) 2, in which the Ca / P ratio was equal to 1.67. The reaction conditions were favorable to the composition of a biomaterial with crystalline phase. The synthesis of the biomaterial composed of hydroxyapatite and alginate microspheres (HAAlg5%; 200 ø 425mm) was obtained from two primary solutions with the aim of, in optimal reactive conditions, to form the precipitate. After synthesis the microspheres were implanted into the defect site. The potential effects of using HAAlg5% and the application of vibratory waves in the critical defect repair were unknown and the results described in this study are promising, considering the systemic therapy and at the site of injury. The biomaterial used promoted repair the injured tissue.

Use of low level laser in nociception control and improvement of the peripheral nerve repair process

2015 ◽  
Vol 12 (3) ◽  
pp. 363
Author(s):  
Daniel Oliveira Martins ◽  
Fabio Martinez dos Santos ◽  
Mara Evany Oliveira ◽  
Luiz R.G. Britto ◽  
José Benedito Dias Lemos ◽  
...  
Keyword(s):  
Peripheral Nerve ◽  
Nerve Repair ◽  
Repair Process ◽  
Peripheral Nerve Repair ◽  
Low Level ◽  
Low Level Laser ◽  
Level Laser

scholarly journals Low-Level Laser Therapy (LLLT) Improves the Repair Process of Peripheral Nerve Injuries: A Mini Review

2017 ◽  
Vol 04 (02) ◽  
Author(s):  
Marcelie Priscila de Oliveira Rosso ◽  
Daniela Vieira Buchaim ◽  
Geraldo Marco Rosa Junior ◽  
Jesus Carlos Andreo ◽  
Karina Torres Pomini ◽  
...  
Keyword(s):  
Peripheral Nerve ◽  
Laser Therapy ◽  
Repair Process ◽  
Low Level Laser Therapy ◽  
Peripheral Nerve Injuries ◽  
Nerve Injuries ◽  
Low Level ◽  
Low Level Laser ◽  
Level Laser

scholarly journals Physical stimulations and their osteogenesis-inducing mechanisms

2018 ◽  
Vol 4 (2) ◽  
Author(s):  
Cijun Shuai ◽  
Wenjing Yang ◽  
Shuping Peng ◽  
Chengde Gao ◽  
Wang Guo ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Mechanical Stimulation ◽  
Bone Repair ◽  
Cell Activity ◽  
Repair Process ◽  
Tissue Growth ◽  
Signal Pathways ◽  
Gene Expressions ◽  
Physical Stimulation ◽  
Pass Through

Physical stimulations such as magnetic, electric and mechanical stimulation could enhance cell activity and promote bone formation in bone repair process via activating signal pathways, modulating ion channels, regulating bone-related gene expressions, etc. In this paper, bioeffects of physical stimulations on cell activity, tissue growth and bone healing were systematically summarized, which especially focused on their osteogenesis-inducing mechanisms. Detailedly, magnetic stimulation could produce Hall effect which improved the permeability of cell membrane and promoted the migration of ions, especially accelerating the extracellular calcium ions to pass through cell membrane. Electric stimulation could induce inverse piezoelectric effect which generated electric signals, accordingly up-regulating intracellular calcium levels and growth factor synthesis. And mechanical stimulation could produce mechanical signals which were converted into corresponding biochemical signals, thus activating various signaling pathways on cell membrane and inducing a series of gene expressions. Besides, the equipments of physical stimulation system were discussed. The opportunities and challenges of physical stimulations were also presented from the perspective of bone repair.

B cell Activity and anti-MBP Autoantibody Response to Peripheral Nerve Injury is Sexually Dimorphic

2020 ◽  
Author(s):  
Hee Jong Lee ◽  
Albert Remacle ◽  
Swathi Hullugundi ◽  
Jennifer Dolkas ◽  
Jake Leung ◽  
...  
Keyword(s):  
Peripheral Nerve ◽  
B Cell ◽  
Nerve Injury ◽  
Peripheral Nerve Injury ◽  
Cell Activity ◽  
Ivig Therapy ◽  
Male Rats ◽  
Sexually Dimorphic ◽  
Autoantibody Production ◽  
Mechanical Hypersensitivity

Abstract Background: Autoantibodies, a hallmark of autoimmune disorders, are thought to contribute to the pathogenesis of chronic pain. Mechanical hypersensitivity is a severe complication of peripheral nerve injury mediated through sex-specific activity of immune and glial cells. Whether B cell contribution to nerve injury and pain is sexually dimorphic remains unknown. Methods: CD20 immunoblotting and immunostaining was conducted in female and male sciatic nerve after chronic constriction injury (CCI). B cell/CD20 targeting by bolus intravenous (IV) Rituximab or control immunoglobulin (IVIG) therapy or vehicle was administered in female and male rats post-CCI, followed by von Frey mechanical sensitivity testing. The cryptic myelin basic protein (MBP) epitopes were identified by immunostaining (EMD-AB5864 Millipore antibody) in female and male CCI nerves. ELISA was used to detect serum autoantibodies against the immunodominant MBP(84-104) epitope was conducted in female and male rats post-CCI. Results: Infiltration of CD20-positive cells, presumably B cell, was observed in female and male nerve at one week post-CCI. A single bolus IV anti-CD20/Rituximab therapy at one week post-CCI reduced the degree of mechanical hypersensitivity in female, but not in male, rats. IVIG therapy and vehicle treatment had no effect in either sex. Sustained release of the cryptic MBP epitopes from myelin was observed in nerves post-CCI in both sexes. Remarkably, serum anti-MBP(84-104) autoantibody was detected in female, but not in male, rats post-CCI. Conclusions: B cell activity and autoantibody production after peripheral nerve injury are sexually dimorphic. In females, mechanical hypersensitivity arising due to autoimmune remodeling of myelin on sensory neuraxis is amenable to immunotherapeutic intervention.

Effect of peripheral nerve stimulation on the dorsal horn cell activity in cats with cutaneous inflammation

1992 ◽  
Vol 33 (2) ◽  
pp. 109 ◽  
Author(s):  
Taick Sang Nam ◽  
Se Hoon Song ◽  
Yeon Hi Kim ◽  
Eun Joo Baik ◽  
Kwnag Se Paik
Keyword(s):  
Peripheral Nerve ◽  
Dorsal Horn ◽  
Nerve Stimulation ◽  
Cell Activity ◽  
Peripheral Nerve Stimulation ◽  
Cutaneous Inflammation

scholarly journals The progress of biomaterials in peripheral nerve repair and regeneration

2020 ◽  
Vol 8 (4) ◽  
pp. 252-269
Author(s):  
Yimeng Wang ◽  
Yuan Zhang ◽  
Xuemin Li ◽  
Qiqing Zhang
Keyword(s):  
Peripheral Nerve ◽  
Nerve Injury ◽  
Nerve Repair ◽  
Repair Process ◽  
Development Strategies ◽  
Post Injury ◽  
Peripheral Nerve Repair ◽  
Advantages And Disadvantages ◽  
Challenging Issue ◽  
Nerve Conduits

Repair and regeneration of the injured peripheral nerve (PN) is a challenging issue in clinics. Although the regeneration outcome of large PN defects is currently unsatisfactory, recently, the study of PN repair has considerably progressed. In particular, biomaterials for repairing PNs, such as nerve guidance conduits and nerve repair membranes, have been well developed. Herein, we summarize the anatomy of the PN, the pathophysiological features of the nerve injury, and the repair process post injury. Then, we highlight the progress in the development of natural and synthetic biomaterials and summarize the applications, advantages, and disadvantages of these materials. These materials can be used as nerve repair membranes and nerve conduits in the field of PN repair. Finally, we discuss the challenges encountered and development strategies for PN repair in the future.

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