scholarly journals Antiproliferative and pro-apoptotic activity of melatonin analogues on melanoma and breast cancer cells

Oncotarget ◽  
2017 ◽  
Vol 8 (40) ◽  
pp. 68338-68353 ◽  
Author(s):  
Giuliana Gatti ◽  
Valeria Lucini ◽  
Silvana Dugnani ◽  
Angela Calastretti ◽  
Gilberto Spadoni ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Apoptotic Activity ◽  
Melatonin Analogues

scholarly journals The Enhanced Cytotoxic and Pro-Apoptotic Effects of Optimized Simvastatin-Loaded Emulsomes on MCF-7 Breast Cancer Cells

Pharmaceutics ◽  
2020 ◽  
Vol 12 (7) ◽  
pp. 597 ◽  
Author(s):  
Zuhier A. Awan ◽  
Usama A. Fahmy ◽  
Shaimaa M. Badr-Eldin ◽  
Tarek S. Ibrahim ◽  
Hani Z. Asfour ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cytotoxic Activity ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Spherical Shape ◽  
Cytotoxic Activities ◽  
Vesicle Size ◽  
Apoptotic Activity ◽  
The Impact ◽  
Mcf 7

Statins, including simvastatin (SMV), are commonly used for the control of hyperlipidaemia and have also proven therapeutic and preventative effects in cardiovascular diseases. Besides that, there is an emerging interest in their use as antineoplastic drugs as demonstrated by different studies showing their cytotoxic activity against different cancer cells. In this study, SMV-loaded emulsomes (SMV-EMLs) were formulated and evaluated for their cytotoxic activity in MCF-7 breast cancer cells. The emulsomes were prepared using a modified thin-film hydration technique. A Box–Behnken model was used to investigate the impact of formulation conditions on vesicle size and drug entrapment. The optimized formulation showed a spherical shape with a vesicle size of 112.42 ± 2.1 nm and an entrapment efficiency of 94.34 ± 1.11%. Assessment of cytotoxic activities indicated that the optimized SMV-EMLs formula exhibited significantly lower half maximal inhibitory concentration (IC50) against MCF-7 cells. Cell cycle analysis indicated the accumulation of cells in the G2-M phase as well as increased cell fraction in the pre-G1 phase, suggesting an enhancement of anti-apoptotic activity of SMV. The staining of cells with Annex V revealed an increase in early and late apoptosis, in line with the increased cellular content of caspase-3 and Bax. In addition, the mitochondrial membrane potential (MMP) was significantly decreased. In conclusion, SMV-EMLs demonstrated superior cell death-inducing activity against MCF-7 cells compared to pure SMV. This is mediated, at least in part, by enhanced pro-apoptotic activity and MMP modulation of SMV.

scholarly journals Knockdown of CD-74 in the Proliferative and Apoptotic Activity of Breast Cancer Cells

2019 ◽  
Vol 7 (19) ◽  
pp. 3169-3176
Author(s):  
Hussain Al Ssadh ◽  
Waleed Al Abdulmonem ◽  
Zafar Rasheed ◽  
Inamul Hasan Madar ◽  
Jamila Alhoderi ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Proliferation ◽  
Cancer Cells ◽  
Cell Lines ◽  
Breast Cancer Cells ◽  
Surrogate Marker ◽  
Tumour Marker ◽  
Breast Cancer Cell Lines ◽  
Proliferation And Apoptosis ◽  
Apoptotic Activity

BACKGROUND: The cluster of differentiation (CD) 74 is known for its immunological functions and its elevated level was reported in various cancer cells. AIM: The aim of the present study was to investigate the expression and potential roles of CD74 in the proliferative and apoptotic activity of breast cancer. METHODS: Expression of CD74, macrophage migration inhibitory factor (MIF) and CD44 was assayed in CAMA-1 and MDA-MB-231 cell lines using flow cytometry. CD74 was knocked down using CD74 siRNA-transfection in CAMA-1, and MDA-MB-231 cells and proliferation and apoptosis were determined in the transfected breast cancer cells. RESULTS: The data showed that CD74, MIF and CD44 were expressed in breast cancer cell lines and were associated with cell proliferation and apoptosis. Correlation analysis revealed that CD74 was positively correlated and colocalised with MIF on the cell-surface of CAMA-1 and MDA-MB-231. The knockdown of CD74 significantly reduced CAMA-1 and MDA-MB-231 cell proliferation and increased the level of apoptotic cells. CONCLUSION: We concluded that the interactions of CD74 with MIF and CD74 with CD44 could be a potential tumour marker for breast cancer cells. Moreover, the level of co-expression of MIF and CD74 or CD44 could be a surrogate marker for the efficacy of anti-angiogenic drugs, particularly in breast cancer tumours. In short, the study revealed the potential roles of CD74 in the proliferation and apoptosis of breast cancer which may serve as a potential therapeutic target for breast cancer.

scholarly journals THE EFFECT OF SOLID LIPID NANOPARTICLES ON TAMOXIFEN-RESISTANT BREAST CANCER CELLS

2016 ◽  
Vol 8 (2) ◽  
pp. 43 ◽  
Author(s):  
Gamze Guney Eskiler ◽  
Gulsah Cecener ◽  
Gokhan Dikmen ◽  
Lutfi Genc ◽  
Unal Egeli
Keyword(s):  
Breast Cancer ◽  
Cell Viability ◽  
Cancer Cells ◽  
Solid Lipid Nanoparticles ◽  
Therapeutic Efficacy ◽  
Breast Cancer Cells ◽  
Lipid Nanoparticles ◽  
Solid Lipid ◽  
Apoptotic Activity

<p class="lead">To overcome the acquired Tamoxifen (Tam) resistance in Tam-resistant breast cancer cells without damaging normal cells, we have examined the therapeutic efficacy of Tam-loaded solid lipid nanoparticles (SLNs). Tam-loaded SLNs were produced by hot homogenization method. After characterization, <em>in vitro</em> cytotoxic and apoptotic activity of Tam-SLNs were evaluated in MCF7, MCF7-TamR (Tam-resistant breast cancer cells) and MCF10A cells. Tam-SLNs had an average size nearly 300 nm and a zeta potential of approximately-40 mV. <em>In vitro</em> cytotoxicity results showed that Tam-SLNs indicated the cytotoxic and apoptotic activity on MCF7 and MCF7-TamR cells. We found that MCF7-TamR cell viability was also suppressed significantly by Tam-SLNs and thus, Tam-SLNs could delay and overcome Tam-resistance (p&lt;0.05). Furthermore, the Tam-SLNs did not induce apoptosis on MCF10A control cells. The lowest MCF10A cell viability was 83.0% whereas MCF7 and MCF7-TamR (R↔ and R↑) cells viability are reduced to 21.98%, 27.5% and 29.4% at 10 µM of Tam-SLNs, respectively (p&lt;0.05). The obtained results were supported by apoptosis assays. SLNs-delivery system provided therapeutic efficacy to overcome Tam-resistance thanks to unique features of SLNs including small size, drug accumulation in the tumor site and controlled drug release. Therefore, Tam-SLNs may have therapeutic potential for the treatment of TAM-resistant breast cancer.</p>

Phosphine/diimine ruthenium complexes with Cl−, CO, NO+, NO2−, NO3− and pyridine ligands: Pro-apoptotic activity on triple-negative breast cancer cells and DNA/HSA interactions

Polyhedron ◽  
2018 ◽  
Vol 144 ◽  
pp. 55-65 ◽  
Author(s):  
Henrique Vieira Reis Silva ◽  
Júlia Scaff Moreira Dias ◽  
Guilherme Álvaro Ferreira-Silva ◽  
Legna Colina Vegas ◽  
Marisa Ionta ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Triple Negative Breast Cancer ◽  
Breast Cancer Cells ◽  
Triple Negative ◽  
Ruthenium Complexes ◽  
Pyridine Ligands ◽  
Apoptotic Activity

Bis(ethyl)norspermine potentiates the apoptotic activity of the pure antiestrogen ICI 182780 in breast cancer cells

2005 ◽  
Author(s):  
Srivani Balabhadrapathruni ◽  
Latha Santhakumaran ◽  
T. Thomas ◽  
Akira Shirahata ◽  
Michael Gallo ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Pure Antiestrogen ◽  
Apoptotic Activity

scholarly journals Tumor homing peptide modified liposomes of capecitabine for improved apoptotic activity and HER2 targeted therapy in breast cancer: in vitro studies

RSC Advances ◽  
2019 ◽  
Vol 9 (43) ◽  
pp. 24987-24994 ◽  
Author(s):  
Mantosh Kumar Singh ◽  
Sai Kiran S. S. Pindiprolu ◽  
Bharat Kumar Reddy Sanapalli ◽  
Vidyasrilekha Yele ◽  
G. N. K. Ganesh
Keyword(s):  
Breast Cancer ◽  
Targeted Therapy ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
In Vitro Studies ◽  
Tumor Homing ◽  
Homing Peptide ◽  
Apoptotic Activity

Tumor homing peptide modified liposomes loaded with capecitabine (CAP) were prepared in the present study. The in vitro efficacy was tested in breast cancer cells.

Cytotoxic and apoptotic activity ofScrophularia oxysepalain MCF-7 human breast cancer cells

2013 ◽  
Vol 95 (7) ◽  
pp. 1208-1220 ◽  
Author(s):  
Samira Valiyari ◽  
Rana Jahanban-Esfahlan ◽  
Fateme Zare Shahneh ◽  
Saeid Yaripour ◽  
Behzad Baradaran ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Human Breast Cancer ◽  
Breast Cancer Cells ◽  
Human Breast Cancer Cells ◽  
Human Breast ◽  
Apoptotic Activity ◽  
Mcf 7

scholarly journals Pro-oxidant and pro-apoptotic activity of polyphenol extract from Annurca apple and its underlying mechanisms in human breast cancer cells

2017 ◽  
Vol 51 (3) ◽  
pp. 939-948 ◽  
Author(s):  
Stefania D'Angelo ◽  
Elisa Martino ◽  
Concetta Paola Ilisso ◽  
Maria Libera Bagarolo ◽  
Marina Porcelli ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Human Breast Cancer ◽  
Breast Cancer Cells ◽  
Human Breast Cancer Cells ◽  
Human Breast ◽  
Underlying Mechanisms ◽  
Apoptotic Activity
Sign in / Sign up

Export Citation Format

Share Document