scholarly journals Tumor homing peptide modified liposomes of capecitabine for improved apoptotic activity and HER2 targeted therapy in breast cancer: in vitro studies

RSC Advances ◽  
2019 ◽  
Vol 9 (43) ◽  
pp. 24987-24994 ◽  
Author(s):  
Mantosh Kumar Singh ◽  
Sai Kiran S. S. Pindiprolu ◽  
Bharat Kumar Reddy Sanapalli ◽  
Vidyasrilekha Yele ◽  
G. N. K. Ganesh
Keyword(s):  
Breast Cancer ◽  
Targeted Therapy ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
In Vitro Studies ◽  
Tumor Homing ◽  
Homing Peptide ◽  
Apoptotic Activity

Tumor homing peptide modified liposomes loaded with capecitabine (CAP) were prepared in the present study. The in vitro efficacy was tested in breast cancer cells.

scholarly journals THE EFFECT OF SOLID LIPID NANOPARTICLES ON TAMOXIFEN-RESISTANT BREAST CANCER CELLS

2016 ◽  
Vol 8 (2) ◽  
pp. 43 ◽  
Author(s):  
Gamze Guney Eskiler ◽  
Gulsah Cecener ◽  
Gokhan Dikmen ◽  
Lutfi Genc ◽  
Unal Egeli
Keyword(s):  
Breast Cancer ◽  
Cell Viability ◽  
Cancer Cells ◽  
Solid Lipid Nanoparticles ◽  
Therapeutic Efficacy ◽  
Breast Cancer Cells ◽  
Lipid Nanoparticles ◽  
Solid Lipid ◽  
Apoptotic Activity

<p class="lead">To overcome the acquired Tamoxifen (Tam) resistance in Tam-resistant breast cancer cells without damaging normal cells, we have examined the therapeutic efficacy of Tam-loaded solid lipid nanoparticles (SLNs). Tam-loaded SLNs were produced by hot homogenization method. After characterization, <em>in vitro</em> cytotoxic and apoptotic activity of Tam-SLNs were evaluated in MCF7, MCF7-TamR (Tam-resistant breast cancer cells) and MCF10A cells. Tam-SLNs had an average size nearly 300 nm and a zeta potential of approximately-40 mV. <em>In vitro</em> cytotoxicity results showed that Tam-SLNs indicated the cytotoxic and apoptotic activity on MCF7 and MCF7-TamR cells. We found that MCF7-TamR cell viability was also suppressed significantly by Tam-SLNs and thus, Tam-SLNs could delay and overcome Tam-resistance (p&lt;0.05). Furthermore, the Tam-SLNs did not induce apoptosis on MCF10A control cells. The lowest MCF10A cell viability was 83.0% whereas MCF7 and MCF7-TamR (R↔ and R↑) cells viability are reduced to 21.98%, 27.5% and 29.4% at 10 µM of Tam-SLNs, respectively (p&lt;0.05). The obtained results were supported by apoptosis assays. SLNs-delivery system provided therapeutic efficacy to overcome Tam-resistance thanks to unique features of SLNs including small size, drug accumulation in the tumor site and controlled drug release. Therefore, Tam-SLNs may have therapeutic potential for the treatment of TAM-resistant breast cancer.</p>

Design and characterization of a recombinant immunotoxin for targeted therapy of breast cancer cells: In vitro and in silico analyses

Life Sciences ◽  
2021 ◽  
Vol 265 ◽  
pp. 118866
Author(s):  
Mahdi Barazesh ◽  
Shiva Mohammadi ◽  
Sajad Jalili ◽  
Soudabeh Kavousipour ◽  
Seyed Nooreddin Faraji ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Targeted Therapy ◽  
Cancer Cells ◽  
In Silico ◽  
Breast Cancer Cells ◽  
Recombinant Immunotoxin ◽  
In Silico Analyses

Gold nanostar–polymer hybrids for siRNA delivery: Polymer design towards colloidal stability and in vitro studies on breast cancer cells

2017 ◽  
Vol 519 (1-2) ◽  
pp. 113-124 ◽  
Author(s):  
Carla Sardo ◽  
Barbara Bassi ◽  
Emanuela F. Craparo ◽  
Cinzia Scialabba ◽  
Elisa Cabrini ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Colloidal Stability ◽  
Sirna Delivery ◽  
In Vitro Studies ◽  
Polymer Hybrids ◽  
Gold Nanostar

N-hexane Extract and Fraction of Green Tea as Antiproliferation of MCM-B2 Breast Cancer Cells In Vitro

2020 ◽  
Vol 6 (2) ◽  
Author(s):  
Lisni Noraida Waruwu ◽  
Maria Bintang ◽  
Bambang Pontjo Priosoeryanto
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Green Tea ◽  
Cancer Cell ◽  
Breast Cancer Cells ◽  
Green Tea Extract ◽  
Hexane Fraction ◽  
Phytochemical Screening ◽  
Tea Extract

Green tea (Camellia sinensis) is one of traditional plants that have the potential as an anticancer. The sample used in this research commercial green tea extract. The purpose of this study was to test the antiproliferation activity of green tea extract on breast cancer cell MCM-B2 in vitro. Green tea extract fractionated using three solvents, ie water, ethanol 70%, and n-hexane. Extract and fraction of green tea water have value Lethality Concentration 50 (LC50) more than 1000 ppm. The fraction of ethanol 70% and n-hexane had an LC50 value of 883.48 ppm and 600.56 ppm, respectively. The results of the phytochemical screening of green tea extract are flavonoids, tannins, and saponins, while the phytochemical screening results of n-hexane fraction are flavonoids and tannins. Antiproliferation activity was tested on breast cancer cells MCM-B2 and normal cells Vero by trypan blue staining method. The highest MCM-B2 cell inhibitory activity was achieved at a concentration of 13000 ppm green tea extract and 1000 ppm of n-hexane fraction, 59% and 59%, respectively. The extract and n-hexane fraction of green tea are not toxic to normal Vero cells characterized by not inhibiting normal cell proliferation. Keywords: antiproliferative, cancer cell MCM-B2, commercial green tea, cytotoxicity

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