scholarly journals Spatial function of the oxidative DNA damage response in radiation induced bystander effects in intra- and inter-system of Caenorhabditis elegans

Oncotarget ◽  
2017 ◽  
Vol 8 (31) ◽  
pp. 51253-51263 ◽  
Author(s):  
Qingqing Li ◽  
Jue Shi ◽  
Lianyun Chen ◽  
Furu Zhan ◽  
Hang Yuan ◽  
...  
Keyword(s):  
Dna Damage ◽  
Caenorhabditis Elegans ◽  
Dna Damage Response ◽  
Oxidative Dna Damage ◽  
Bystander Effects ◽  
Spatial Function ◽  
Damage Response ◽  
Radiation Induced

scholarly journals In Barrett’s epithelial cells, weakly acidic bile salt solutions cause oxidative DNA damage with response and repair mediated by p38

2020 ◽  
Vol 318 (3) ◽  
pp. G464-G478
Author(s):  
Xiaofang Huo ◽  
Kerry B. Dunbar ◽  
Xi Zhang ◽  
Qiuyang Zhang ◽  
Stuart Jon Spechler ◽  
...  
Keyword(s):  
Dna Damage ◽  
Bile Salt ◽  
Barrett’S Esophagus ◽  
Esophageal Adenocarcinoma ◽  
Dna Damage Response ◽  
Barrett's Esophagus ◽  
Oxidative Dna Damage ◽  
Damage Response ◽  
Barrett's Metaplasia ◽  
Barrett’S Metaplasia

The frequency of esophageal adenocarcinoma is rising despite widespread use of proton pump inhibitors (PPIs), which heal reflux esophagitis but do not prevent reflux of weakly acidic gastric juice and bile in Barrett’s esophagus patients. We aimed to determine if weakly acidic (pH 5.5) bile salt medium (WABM) causes DNA damage in Barrett’s cells. Because p53 is inactivated frequently in Barrett’s esophagus and p38 can assume p53 functions, we explored p38’s role in DNA damage response and repair. We exposed Barrett’s cells with or without p53 knockdown to WABM, and evaluated DNA damage, its response and repair, and whether these effects are p38 dependent. We also measured phospho-p38 in biopsies of Barrett’s metaplasia exposed to deoxycholic acid (DCA). WABM caused phospho-H2AX increases that were blocked by a reactive oxygen species (ROS) scavenger. WABM increased phospho-p38 and reduced bromodeoxyuridine incorporation (an index of S phase entry). Repair of WABM-induced DNA damage proceeded through p38-mediated base excision repair (BER) associated with reduction-oxidation factor 1-apurinic/apyrimidinic endonuclease I (Ref-1/APE1). Cells treated with WABM supplemented with ursodeoxycholic acid (UDCA) exhibited enhanced p38-mediated responses to DNA damage. All of these effects were observed in p53-intact and p53-deficient Barrett’s cells. In patients, esophageal DCA perfusion significantly increased phospho-p38 in Barrett’s metaplasia. WABM exposure generates ROS, causing oxidative DNA damage in Barrett’s cells, a mechanism possibly underlying the rising frequency of esophageal adenocarcinoma despite PPI usage. p38 plays a central role in oxidative DNA damage response and Ref-1/APE1-associated BER, suggesting potential chemopreventive roles for agents like UDCA that increase p38 activity in Barrett’s esophagus. NEW & NOTEWORTHY We found that weakly acidic bile salt solutions, with compositions similar to the refluxed gastric juice of gastroesophageal reflux disease patients on proton pump inhibitors, cause oxidative DNA damage in Barrett’s metaplasia that could contribute to the development of esophageal adenocarcinoma. We also have elucidated a critical role for p38 in Barrett’s metaplasia in its response to and repair of oxidative DNA damage, suggesting a potential chemopreventive role for agents like ursodeoxycholic acid that increase p38 activity in Barrett’s esophagus.

Radiation-induced DNA damage response and resistance in colorectal cancer stem-like cells

2019 ◽  
Vol 95 (6) ◽  
pp. 667-679 ◽  
Author(s):  
Kumari Anuja ◽  
Amit Roy Chowdhury ◽  
Arka Saha ◽  
Souvick Roy ◽  
Arabinda Kumar Rath ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Dna Damage ◽  
Dna Damage Response ◽  
Damage Response ◽  
Radiation Induced

scholarly journals A simple answer to complex questions: Caenorhabditis elegans as an experimental model for examining the DNA damage response and disease genes

2017 ◽  
Vol 233 (4) ◽  
pp. 2781-2790 ◽  
Author(s):  
Matthias Rieckher ◽  
Arturo Bujarrabal ◽  
Markus A. Doll ◽  
Najmeh Soltanmohammadi ◽  
Björn Schumacher
Keyword(s):  
Dna Damage ◽  
Caenorhabditis Elegans ◽  
Experimental Model ◽  
Dna Damage Response ◽  
Disease Genes ◽  
Damage Response

Ionizing radiation-induced DNA damage response identified in marine mussels, Mytilus sp.

2012 ◽  
Vol 168 ◽  
pp. 107-112 ◽  
Author(s):  
Ohoud D. AlAmri ◽  
Andrew B. Cundy ◽  
Yanan Di ◽  
Awadhesh N. Jha ◽  
Jeanette M. Rotchell
Keyword(s):  
Dna Damage ◽  
Ionizing Radiation ◽  
Dna Damage Response ◽  
Damage Response ◽  
Marine Mussels ◽  
Radiation Induced

Altered DNA damage response in Caenorhabditis elegans with impaired poly(ADP-ribose) glycohydrolases genes expression

DNA Repair ◽  
2007 ◽  
Vol 6 (3) ◽  
pp. 329-343 ◽  
Author(s):  
Jean-François St-Laurent ◽  
Steve N. Gagnon ◽  
Florence Dequen ◽  
Isabelle Hardy ◽  
Serge Desnoyers
Keyword(s):  
Dna Damage ◽  
Caenorhabditis Elegans ◽  
Dna Damage Response ◽  
Genes Expression ◽  
Damage Response
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