scholarly journals Suppression of PC-1/PrLZ sensitizes prostate cancer cells to ionizing radiation by attenuating DNA damage repair and inducing autophagic cell death

Oncotarget ◽  
2016 ◽  
Vol 7 (38) ◽  
pp. 62340-62351 ◽  
Author(s):  
Zeng-Fu Shang ◽  
Qiang Wei ◽  
Lan Yu ◽  
Fang Huang ◽  
Bei-Bei Xiao ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Dna Damage ◽  
Cell Death ◽  
Ionizing Radiation ◽  
Cancer Cells ◽  
Dna Damage Repair ◽  
Autophagic Cell Death ◽  
Prostate Cancer Cells ◽  
Damage Repair

scholarly journals Zerumbone Regulates DNA Repair Responding to Ionizing Radiation and Enhances Radiosensitivity of Human Prostatic Cancer Cells

2017 ◽  
Vol 17 (2) ◽  
pp. 292-298 ◽  
Author(s):  
Pai-Kai Chiang ◽  
Wei-Kung Tsai ◽  
Marcelo Chen ◽  
Wun-Rong Lin ◽  
Yung-Chiong Chow ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Dna Damage ◽  
Ionizing Radiation ◽  
Cancer Cells ◽  
Prostatic Cancer ◽  
Radiation Sensitivity ◽  
Human Prostate Cancer ◽  
Prostate Cancer Cells ◽  
Damage Repair ◽  
Radiation Induced

Introduction. Radiation therapy using ionizing radiation is widely used for the treatment of prostate cancer. The intrinsic radiation sensitivity of cancer cells could be enhanced by modulating multiple factors including the capacity to repair DNA damage, especially double-strand breaks (DSBs). We aimed to examine the effect of zerumbone on radiation sensitivity and its protective effects against ionizing radiation–induced DSB in human prostate cancer cells. Materials and Methods. The human prostate cancer PC3 and DU145 cell lines were used. A colony formation assay was performed to analyze the radiation survival of cells. DNA histogram and generation of reactive oxygen species (ROS) were examined using flow cytometry. Western blotting was used to examine the expression of regulatory molecules related to DNA damage repair. Results. Pretreatment with zerumbone enhanced the radiation effect on prostate cancer cells. Zerumbone delayed the abrogation of radiation-induced expression of γ-H2AX, an indicator of DNA DSB. Zerumbone pretreatment markedly reduced ionizing radiation–induced upregulated expression of phosphorylated ATM (ataxia telangiectasia-mutated), which was partially reversed by the ATM agonist methyl methanesulfonate. Ionizing radiation augmented and zerumbone pretreatment reduced the expression of Jak2 and Stat3, which are involved in DNA damage repair signaling. No significant effect on the generation of ROS and expression of ATR was noted after zerumbone treatment. Conclusion: Zerumbone sensitized DU145 and PC3 prostatic cancer cells to ionizing radiation by modulating radiation-induced ATM activation during repair of DNA DSBs.

scholarly journals Cyclin D1 Promotes Androgen-Dependent DNA Damage Repair in Prostate Cancer Cells

2015 ◽  
Vol 76 (2) ◽  
pp. 329-338 ◽  
Author(s):  
Mathew C. Casimiro ◽  
Gabriele Di Sante ◽  
Xiaoming Ju ◽  
Zhiping Li ◽  
Ke Chen ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Dna Damage ◽  
Cyclin D1 ◽  
Cancer Cells ◽  
Dna Damage Repair ◽  
Prostate Cancer Cells ◽  
Damage Repair

Pseudolaric Acid B Induces Caspase-Dependent Apoptosis and Autophagic Cell Death in Prostate Cancer Cells

2012 ◽  
Vol 27 (6) ◽  
pp. 885-891 ◽  
Author(s):  
Jing Tong ◽  
Shutao Yin ◽  
Yinhui Dong ◽  
Xiao Guo ◽  
Lihong Fan ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Cell Death ◽  
Cancer Cells ◽  
Autophagic Cell Death ◽  
Prostate Cancer Cells ◽  
Pseudolaric Acid B
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