scholarly journals Modeling paraquat-induced lung fibrosis in C. elegans reveals KRIT1 as a key regulator of collagen gene transcription

Aging ◽  
2021 ◽  
Author(s):  
Gongping Deng ◽  
Le Li ◽  
Yanhong Ouyang
Keyword(s):  
Gene Transcription ◽  
Lung Fibrosis ◽  
Collagen Gene ◽  
C Elegans

scholarly journals Number and organization of collagen genes in Caenorhabditis elegans.

1984 ◽  
Vol 4 (11) ◽  
pp. 2389-2395 ◽  
Author(s):  
G N Cox ◽  
J M Kramer ◽  
D Hirsh
Keyword(s):  
Caenorhabditis Elegans ◽  
Southern Blot ◽  
Blot Hybridization ◽  
Southern Blot Hybridization ◽  
Phage Library ◽  
Collagen Gene ◽  
Recombinant Phage ◽  
C Elegans ◽  
Dna Libraries ◽  
Collagen Genes

We analyzed the number and organization of collagen genes in the nematode Caenorhabditis elegans. Genomic Southern blot hybridization experiments and recombinant phage library screenings indicated that C. elegans has between 40 and 150 distinct collagen genes. A large number of recombinant phages containing collagen genes were isolated from C. elegans DNA libraries. Physical mapping studies indicated that most phage contained a single small collagen gene less than 3 kilobases in size. A few phage contained multiple collagen hybridizing regions and may contain a larger collagen gene or several tightly linked small collagen genes. No overlaps were observed between phages containing different collagen genes, implying that the genes are dispersed in the C. elegans genome. Consistent with the small size of most collagen genes, we found that the predominant class of collagen mRNA in C. elegans is 1.2 to 1.4 kilobases in length. Genomic Southern blot experiments under stringent hybridization conditions revealed considerable sequence diversity among collagen genes. Our data suggest that most collagen genes are unique or are present in only a few copies.

Changes in albumin, α-fetoprotein and collagen gene transcription in ccl4-induced hepatic fibrosis

Hepatology ◽  
1988 ◽  
Vol 8 (2) ◽  
pp. 259-266 ◽  
Author(s):  
Arturo Panduro ◽  
Fouad Shalaby ◽  
Luis Biempica ◽  
David A. Shafritz
Keyword(s):  
Hepatic Fibrosis ◽  
Gene Transcription ◽  
Collagen Gene

scholarly journals Gene transcription is coordinated with, but not dependent on, cell divisions during C. elegans embryonic fate specification

Development ◽  
2013 ◽  
Vol 140 (16) ◽  
pp. 3385-3394 ◽  
Author(s):  
G. Nair ◽  
T. Walton ◽  
J. I. Murray ◽  
A. Raj
Keyword(s):  
Gene Transcription ◽  
Fate Specification ◽  
C Elegans ◽  
Cell Divisions

scholarly journals GCN-2 dependent inhibition of protein synthesis activates osmosensitive gene transcription via WNK and Ste20 kinase signaling

2012 ◽  
Vol 303 (12) ◽  
pp. C1269-C1277 ◽  
Author(s):  
Elaine Choung-Hee Lee ◽  
Kevin Strange
Keyword(s):  
Protein Synthesis ◽  
Gene Transcription ◽  
Kinase Signaling ◽  
Eukaryotic Translation ◽  
Hypertonic Stress ◽  
C Elegans ◽  
Initiation Factors ◽  
Inhibition Of Protein Synthesis ◽  
Genes Encoding ◽  
Eukaryotic Translation Initiation Factors

Increased gpdh-1 transcription is required for accumulation of the organic osmolyte glycerol and survival of Caenorhabditis elegans during hypertonic stress. Our previous work has shown that regulators of gpdh-1 ( rgpd) gene knockdown constitutively activates gpdh-1 expression. Fifty-five rgpd genes play essential roles in translation suggesting that inhibition of protein synthesis is an important signal for regulating osmoprotective gene transcription. We demonstrate here that translation is reduced dramatically by hypertonic stress or knockdown of rgpd genes encoding aminoacyl-tRNA synthetases and eukaryotic translation initiation factors (eIFs). Toxin-induced inhibition of translation also activates gpdh-1 expression. Hypertonicity-induced translation inhibition is mediated by general control nonderepressible (GCN)-2 kinase signaling and eIF-2α phosphoryation. Loss of gcn-1 or gcn-2 function prevents eIF-2α phosphorylation, completely blocks reductions in translation, and inhibits gpdh-1 transcription. gpdh-1 expression is regulated by the highly conserved with-no-lysine kinase (WNK) and Ste20 kinases WNK-1 and GCK-3, which function in the GCN-2 signaling pathway downstream from eIF-2α phosphorylation. Our previous work has shown that hypertonic stress causes rapid and dramatic protein damage in C. elegans and that inhibition of translation reduces this damage. The current studies demonstrate that reduced translation also serves as an essential signal for activation of WNK-1/GCK-3 kinase signaling and subsequent transcription of gpdh-1 and possibly other osmoprotective genes.

scholarly journals C. elegans ELT-3 regulates cuticle collagen expression in response to environmental stimuli

2020 ◽  
Author(s):  
Hiva Mesbahi ◽  
Kim B. Pho ◽  
Andrea J. Tench ◽  
Victoria L. Leon Guerrero ◽  
Lesley T. MacNeil
Keyword(s):  
Gene Expression ◽  
Collagen Gene ◽  
Missense Mutations ◽  
Environmental Stimuli ◽  
C Elegans ◽  
Collagen Expression ◽  
Collagen Gene Expression ◽  
Collagen Genes ◽  
Early Developmental

AbstractThe nematode Caenorhabditis elegans is protected from the environment by the cuticle, an extracellular collagen-based matrix that encloses the animal. Over 170 cuticular collagens are predicted in the C. elegans genome, but the role of each individual collagen is unclear. Stage-specific specialization of the cuticle explains the need for some collagens, however, the large number of collagens suggests that specialization of the cuticle may also occur in response to other environmental triggers. Missense mutations in many collagen genes can disrupt cuticle morphology, producing a helically twisted body causing the animal to move in a stereotypical pattern described as rolling. We find that environmental factors, including diet, early developmental arrest, and population density can differentially influence the penetrance of rolling in these mutants. These effects are in part due to changes in collagen gene expression that are mediated by the GATA family transcription factor ELT-3. We propose a model by which ELT-3 regulates collagen gene expression in response to environmental stimuli to promote the assembly of a cuticle specialized to a given environment.

scholarly journals Prdm5 Regulates Collagen Gene Transcription by Association with RNA Polymerase II in Developing Bone

PLoS Genetics ◽  
2012 ◽  
Vol 8 (5) ◽  
pp. e1002711 ◽  
Author(s):  
Giorgio Giacomo Galli ◽  
Kristian Honnens de Lichtenberg ◽  
Matteo Carrara ◽  
Wolfgang Hans ◽  
Manuela Wuelling ◽  
...  
Keyword(s):  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Gene Transcription ◽  
Collagen Gene

scholarly journals Interaction between GC Box Binding Factors and Smad Proteins Modulates Cell Lineage-specific α2(I) Collagen Gene Transcription

2001 ◽  
Vol 276 (19) ◽  
pp. 16573-16579 ◽  
Author(s):  
Yutaka Inagaki ◽  
Tomoyuki Nemoto ◽  
Atsuhito Nakao ◽  
Peter ten Dijke ◽  
Kenichi Kobayashi ◽  
...  
Keyword(s):  
Gene Transcription ◽  
Cell Lineage ◽  
Collagen Gene ◽  
Smad Proteins ◽  
Gc Box
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