scholarly journals MiR-520d-5p modulates chondrogenesis and chondrocyte metabolism through targeting HDAC1

Aging ◽  
2020 ◽  
Vol 12 (18) ◽  
pp. 18545-18560
Author(s):  
Jiajia Lu ◽  
Zhibin Zhou ◽  
Bin Sun ◽  
Bin Han ◽  
Qiang Fu ◽  
...  
Keyword(s):  
Chondrocyte Metabolism

scholarly journals Sirt5 deficiency causes post-translational protein malonylation and dysregulated cellular metabolism in chondrocytes under obesity conditions

2020 ◽  
Author(s):  
Shouan Zhu ◽  
Albert Batushansky ◽  
Anita Jopkiewicz ◽  
Dawid Makosa ◽  
Kenneth M. Humphries ◽  
...  
Keyword(s):  
Cellular Metabolism ◽  
Tca Cycle ◽  
Gas Chromatography Mass Spectrometry ◽  
Dependent Manner ◽  
Joint Injury ◽  
Primary Chondrocytes ◽  
Wide Range ◽  
Chondrocyte Metabolism ◽  
High Concentrations

ABSTRACTObjectiveObesity accelerates the development of osteoarthritis (OA) during aging and is associated with altered chondrocyte cellular metabolism. The objective of this study was to investigate the role of sirtuin 5 (SIRT5) in regulating chondrocyte protein lysine malonylation (MaK) and cellular metabolism under obesity-related conditions.MethodsMaK and SIRT5 were immunostained in knee articular cartilage of obese db/db mice and different aged C57BL6 mice with or without destabilization of the medial meniscus (DMM) surgery to induce OA. Primary chondrocytes were isolated from 7-day-old WT and Sirt5−/− mice and treated with varying concentrations of glucose and insulin to mimic obesity. Sirt5-dependent effects on MaK and metabolism were evaluated by Western blot, Seahorse Respirometry, and gas/chromatography-mass/spectrometry (GC-MS) metabolic profiling.ResultsMaK was significantly increased in cartilage of db/db mice and in chondrocytes treated with high concentrations of glucose and insulin (GluhiInshi). Sirt5 protein was increased in an age-dependent manner following joint injury, and Sirt5 deficient primary chondrocytes had increased MaK, decreased glycolysis rate, and reduced basal mitochondrial respiration. GC-MS identified 41 metabolites. Sirt5 deficiency altered 13 distinct metabolites under basal conditions and 18 metabolites under GluhiInshi treatment. Pathway analysis identified a wide range of Sirt5-dependent altered metabolic pathways that include amino acid metabolism, TCA cycle, and glycolysis.ConclusionThis study provides the first evidence that Sirt5 broadly regulates chondrocyte metabolism. We observed changes in Sirt5 and MaK levels in cartilage with obesity and joint injury, suggesting that the Sirt5-MaK pathway may contribute to altered chondrocyte metabolism that occurs during OA development.

scholarly journals Pathomechanisms of Posttraumatic Osteoarthritis: Chondrocyte Behavior and Fate in a Precarious Environment

2020 ◽  
Vol 21 (5) ◽  
pp. 1560 ◽  
Author(s):  
Jana Riegger ◽  
Rolf E. Brenner
Keyword(s):  
Oxidative Stress ◽  
Therapeutic Strategies ◽  
Traumatic Injuries ◽  
Posttraumatic Osteoarthritis ◽  
Catabolic Enzymes ◽  
Regulated Cell Death ◽  
Chondrocyte Metabolism ◽  
Molecular Patterns ◽  
Damage Associated Molecular Patterns ◽  
Novel Therapeutic

Traumatic injuries of the knee joint result in a wide variety of pathomechanisms, which contribute to the development of so-called posttraumatic osteoarthritis (PTOA). These pathogenetic processes include oxidative stress, excessive expression of catabolic enzymes, release of damage-associated molecular patterns (DAMPs), and synovial inflammation. The present review focuses on the underlying pathomechanisms of PTOA and in particular the behavior and fate of the surviving chondrocytes, comprising chondrocyte metabolism, regulated cell death, and phenotypical changes comprising hypertrophy and senescence. Moreover, possible therapeutic strategies, such as chondroanabolic stimulation, anti-oxidative and anti-inflammatory treatment, as well as novel therapeutic targets are discussed.

Effects of intermittent hydrostatic pressure and BMP-2 on osteoarthritic human chondrocyte metabolism in vitro

2010 ◽  
Vol 29 (3) ◽  
pp. 361-368 ◽  
Author(s):  
R.L. Smith ◽  
D.P. Lindsey ◽  
L. Dhulipala ◽  
A.H.S. Harris ◽  
S.B. Goodman ◽  
...  
Keyword(s):  
Hydrostatic Pressure ◽  
Human Chondrocyte ◽  
Chondrocyte Metabolism

Chondrocyte metabolism as affected by vitamin A

1975 ◽  
Vol 19 (1) ◽  
pp. 99-107 ◽  
Author(s):  
N. S. Vasan ◽  
J. W. Lash
Keyword(s):  
Vitamin A ◽  
Chondrocyte Metabolism
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