scholarly journals Micro- and nanoscale characterization of different natural biomaterials for ocular surface regeneration

2020 ◽  
Author(s):  
Gamze DERELİ CAN ◽  
Atakan TEVLEK ◽  
Mehmet Erol CAN ◽  
Elif ÖNCÜ ◽  
Halil Murat AYDIN ◽  
...  
Keyword(s):  
Ocular Surface ◽  
Nanoscale Characterization ◽  
Surface Regeneration

scholarly journals Characterization of Ocular Surface Microbial Profiles Revealed Discrepancies between Conjunctival and Corneal Microbiota

Pathogens ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 405
Author(s):  
Anna Matysiak ◽  
Michal Kabza ◽  
Justyna A. Karolak ◽  
Marcelina M. Jaworska ◽  
Malgorzata Rydzanicz ◽  
...  
Keyword(s):  
Microbial Communities ◽  
Ocular Surface ◽  
Molecular Techniques ◽  
Corneal Tissue ◽  
Rna Seq ◽  
Microbiome Composition ◽  
Viral Sequences ◽  
Pcr Techniques ◽  
Unmapped Reads

The ocular microbiome composition has only been partially characterized. Here, we used RNA-sequencing (RNA-Seq) data to assess microbial diversity in human corneal tissue. Additionally, conjunctival swab samples were examined to characterize ocular surface microbiota. Short RNA-Seq reads, obtained from a previous transcriptome study of 50 corneal tissues, were mapped to the human reference genome GRCh38 to remove sequences of human origin. The unmapped reads were then used for taxonomic classification by comparing them with known bacterial, archaeal, and viral sequences from public databases. The components of microbial communities were identified and characterized using both conventional microbiology and polymerase chain reaction (PCR) techniques in 36 conjunctival swabs. The majority of ocular samples examined by conventional and molecular techniques showed very similar microbial taxonomic profiles, with most of the microorganisms being classified into Proteobacteria, Firmicutes, and Actinobacteria phyla. Only 50% of conjunctival samples exhibited bacterial growth. The PCR detection provided a broader overview of positive results for conjunctival materials. The RNA-Seq assessment revealed significant variability of the corneal microbial communities, including fastidious bacteria and viruses. The use of the combined techniques allowed for a comprehensive characterization of the eye microbiome’s elements, especially in aspects of microbiota diversity.

Characterization of Ocular Surface Symptoms From Optometric Practices in North America

Cornea ◽  
2001 ◽  
Vol 20 (6) ◽  
pp. 610-618 ◽  
Author(s):  
Carolyn G. Begley ◽  
Robin L. Chalmers ◽  
G. Lynn Mitchell ◽  
Kelly K. Nichols ◽  
Barbara Caffery ◽  
...  
Keyword(s):  
North America ◽  
Ocular Surface

Nanoscale characterization of powder materials by atom probe tomography

2011 ◽  
Vol 208 (2) ◽  
pp. 260-265 ◽  
Author(s):  
R. Larde ◽  
J. Bran ◽  
M. Jean ◽  
J.M. Le Breton
Keyword(s):  
Atom Probe Tomography ◽  
Atom Probe ◽  
Powder Materials ◽  
Nanoscale Characterization

Nanoscale characterization of silica soots and aluminium solution doping in optical fibre fabrication

2006 ◽  
Vol 352 (36-37) ◽  
pp. 3799-3807 ◽  
Author(s):  
F.Z. Tang ◽  
P. McNamara ◽  
G.W. Barton ◽  
S.P. Ringer
Keyword(s):  
Optical Fibre ◽  
Nanoscale Characterization ◽  
Solution Doping

Nanoscale characterization of bone–implant interface and biomechanical modulation of bone ingrowth

2007 ◽  
Vol 27 (3) ◽  
pp. 382-393 ◽  
Author(s):  
Paul A. Clark ◽  
Andrew M. Clark ◽  
Anthony Rodriguez ◽  
Mohammad A. Hussain ◽  
Jeremy J. Mao
Keyword(s):  
Bone Ingrowth ◽  
Bone Implant ◽  
Nanoscale Characterization

Advances in nanoscale characterization of refined nanoporous gold

2018 ◽  
Vol 283 ◽  
pp. 611-618 ◽  
Author(s):  
Ayman A. El-Zoka ◽  
Brian Langelier ◽  
Andreas Korinek ◽  
Gianluigi A. Botton ◽  
Roger C. Newman
Keyword(s):  
Nanoporous Gold ◽  
Nanoscale Characterization
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