scholarly journals Human adipose-derived stem cells obtained from lipoaspirates are highly susceptible to hydrogen peroxide mediated cytogenotoxicity

2019 ◽  
Vol 1 (1) ◽  
pp. 11-28
Author(s):  
Alencar Kolinski Machado ◽  
Sabrina Guastavino Homrich ◽  
Cíntia Corte Real Rodrigues ◽  
Verônica Farina Azzolin ◽  
Marta Maria Medeiros Frescura Duarte ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Stem Cells ◽  
Safety Profile ◽  
Mortality Rates ◽  
Adipose Derived Stem Cells ◽  
Apoptosis Induction ◽  
Genotoxic Effects ◽  
Dna Comet Assay ◽  
Stress Markers ◽  
Highly Sensitive

There is evidence that H2O2 can induce the proliferation, migration, and regeneration of stem cells, as well as that of adipose-derived stem cells (ASCs). This could be useful to expand the possible uses of ASCs in therapeutic applications.  However, the safety profile of H2O2 use in stem cells is not clear yet. Therefore, the present study evaluated the acute cytotoxic, oxidative and genotoxic effects of different concentrations of H2O2 on ASCs obtained from human lipoaspirates. The ASCs were treated with 1–1000 μM H2O2 for two hours. Cell viability was evaluated by double-strand DNA determination. Apoptosis induction was analyzed measuring active levels of caspases 1, 3 and 8. Biochemical oxidative stress markers were analyzed and genotoxic effects were assessed by DNA comet assay. All H2O2 concentrations increased ASC mortality rates with approximately 100% mortality achieved at ≥ 200 μM. Active caspases 1, 3 and 8, oxidative stress, as well as oxidative damage as assessed by lipid peroxidation increased dose‐dependently. There was also an approximate 50% increase in catalase levels in cells exposed to all H2O2 tested concentrations. H2O2 concentrations of ≥ 10 μM were genotoxic. These results suggest that ASCs are highly sensitive to H2O2 exposition. In addition, DNA damage in the surviving cells may affect their proliferative and differentiation capacity, as well as their safety profile for therapeutic use.

scholarly journals Hydrogen Peroxide-Preconditioned Human Adipose-Derived Stem Cells Enhance the Recovery of Oligodendrocyte-Like Cells after Oxidative Stress-Induced Damage

2020 ◽  
Vol 21 (24) ◽  
pp. 9513
Author(s):  
Patricia Garrido-Pascual ◽  
Ana Alonso-Varona ◽  
Begoña Castro ◽  
María Burón ◽  
Teodoro Palomares
Keyword(s):  
Oxidative Stress ◽  
Stem Cells ◽  
Therapeutic Potential ◽  
Mild Stress ◽  
Adipose Derived Stem Cells ◽  
Vitro Model ◽  
Novel Strategy ◽  
The One ◽  
Cell Conditioned Medium

Oxidative stress associated with neuroinflammation is a key process involved in the pathophysiology of neurodegenerative diseases, and therefore, has been proposed as a crucial target for new therapies. Recently, the therapeutic potential of human adipose-derived stem cells (hASCs) has been investigated as a novel strategy for neuroprotection. These cells can be preconditioned by exposing them to mild stress in order to improve their response to oxidative stress. In this study, we evaluate the therapeutic potential of hASCs preconditioned with low doses of H2O2 (called HC016 cells) to overcome the deleterious effect of oxidative stress in an in vitro model of oligodendrocyte-like cells (HOGd), through two strategies: i, the culture of oxidized HOGd with HC016 cell-conditioned medium (CM), and ii, the indirect co-culture of oxidized HOGd with HC016 cells, which had or had not been exposed to oxidative stress. The results demonstrated that both strategies had reparative effects, oxidized HC016 cell co-culture being the one associated with the greatest recovery of the damaged HOGd, increasing their viability, reducing their intracellular reactive oxygen species levels and promoting their antioxidant capacity. Taken together, these findings support the view that HC016 cells, given their reparative capacity, might be considered an important breakthrough in cell-based therapies.

Opposite Effects of Mechanical Action of Fluid Flow on Proangiogenic Factor Secretion From Human Adipose-Derived Stem Cells With and Without Oxidative Stress

2017 ◽  
Vol 232 (8) ◽  
pp. 2158-2167 ◽  
Author(s):  
Beatriz Bravo ◽  
Cira García de Durango ◽  
Álvaro González ◽  
Arancha R. Gortázar ◽  
Xavier Santos ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Stem Cells ◽  
Fluid Flow ◽  
Mechanical Action ◽  
Adipose Derived Stem Cells ◽  
Factor Secretion

scholarly journals Extracts from Myrtle Liqueur Processing Waste Modulate Stem Cells Pluripotency under Stressing Conditions

2019 ◽  
Vol 2019 ◽  
pp. 1-12 ◽  
Author(s):  
Sara Cruciani ◽  
Sara Santaniello ◽  
Angela Fadda ◽  
Luana Sale ◽  
Giorgia Sarais ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Stem Cells ◽  
Stem Cell ◽  
Pharmaceutical Preparations ◽  
Ros Production ◽  
Adipose Derived Stem Cells ◽  
Epigenetic Changes ◽  
Processing Waste ◽  
Stem Cell Pluripotency

Nutraceuticals present in food are molecules able to exert biological activity for the prevention and treatment of various diseases, in form of pharmaceutical preparations, such as capsules, cream, or pills. Myrtus communis L. is a spontaneous Mediterranean evergreen shrub, widely known for the liqueur obtained from its berries rich in phytochemicals such as tannins and flavonoids. In the present study, we aimed to evaluate the properties of myrtle byproducts, residual of the industrial liqueur processing, in Adipose-derived stem cells (ADSCs) induced at oxidative stress by in vitro H2O2 treatment. Cells were exposed for 12-24 and 48h at treatment with extracts and then senescence-induced. ROS production was then determined. The real-time PCR was performed to evaluate the expression of inflammatory cytokines and sirtuin-dependent epigenetic changes, as well the modifications in terms of stem cell pluripotency. The β-galactosidase assay was conducted to analyze stem cell senescence after treatment. Our results show that industrial myrtle byproducts retain a high antioxidant and antisenescence activity, protecting cells from oxidative stress damages. The results obtained suggest that residues from myrtle liqueur production could be used as resource in formulation of food supplements or pharmaceutical preparations with antioxidant, antiaging, and anti-inflammatory activity.

Evidence supporting neuroprotective effect of adipose derived stem cells on PC12 cells against oxidative stress induced by H2O2

2017 ◽  
Vol 63 (3) ◽  
pp. 1 ◽  
Author(s):  
M. T. Ghorabi ◽  
A. Aliaghaei ◽  
Y. Sadeghi ◽  
F. Shaerzadeh ◽  
A. A. Rad ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Stem Cells ◽  
Pc12 Cells ◽  
Neuroprotective Effect ◽  
Adipose Derived Stem Cells

scholarly journals Released from ZrO2/SiO2 coating resveratrol inhibits senescence and oxidative stress of human adipose-derived stem cells (ASC)

2018 ◽  
Vol 16 (1) ◽  
pp. 481-495 ◽  
Author(s):  
K. Kornicka ◽  
R. Walczak ◽  
A. Mucha ◽  
K. Marycz
Keyword(s):  
Oxidative Stress ◽  
Stem Cells ◽  
Elderly Patients ◽  
Bone Fractures ◽  
Pure Titanium ◽  
Titanium Substrate ◽  
Stress Factors ◽  
Material Surface ◽  
Adipose Derived Stem Cells

AbstractThe rapid aging of the population results in increased number of metabolic and degenerative disorders, especially in the elderly.Thus, a novel approach in the fields of orthopedic and reconstructive surgery for bone regeneration is strongly desirable. A new perspective in the therapy of bone fractures is tissue engineering which combines living cells with biomaterials to develop modern substitutes that can restore tissue functions. Metallic biomaterials, including stainless steel and pure titanium, have been extensively used for the fabrication of surgical implants over decades. Chemical modification of material surface for example incorporation of chemotactic factors may significantly improve the therapeutic effect. In this paper we describe titanium substrate modifications with ZrO2/SiO2 coating functionalized with resveratrol using a sol – gel, dip-coating technique. Moreover, we established the effects of fabricated scaffolds on adipose stem cells isolated from elderly patients. Using fluorescence imaging, polymerase chain reaction (PCR)and cytotoxicity tests, we established that 0.5 Res_ZrO2/SiO2 significantly reduced apoptosis and accumulation of oxidative stress factors in adipose derived stem cells (ASC). Thus exploitation of fabricated biomaterials in regenerative medicine as a strategy for rejuvenate ASC from elderly patients in vivo, seems fully justified.

Sign in / Sign up

Export Citation Format

Share Document