Opposite Effects of Mechanical Action of Fluid Flow on Proangiogenic Factor Secretion From Human Adipose-Derived Stem Cells With and Without Oxidative Stress

2017 ◽  
Vol 232 (8) ◽  
pp. 2158-2167 ◽  
Author(s):  
Beatriz Bravo ◽  
Cira García de Durango ◽  
Álvaro González ◽  
Arancha R. Gortázar ◽  
Xavier Santos ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Stem Cells ◽  
Fluid Flow ◽  
Mechanical Action ◽  
Adipose Derived Stem Cells ◽  
Factor Secretion

scholarly journals Hydrogen Peroxide-Preconditioned Human Adipose-Derived Stem Cells Enhance the Recovery of Oligodendrocyte-Like Cells after Oxidative Stress-Induced Damage

2020 ◽  
Vol 21 (24) ◽  
pp. 9513
Author(s):  
Patricia Garrido-Pascual ◽  
Ana Alonso-Varona ◽  
Begoña Castro ◽  
María Burón ◽  
Teodoro Palomares
Keyword(s):  
Oxidative Stress ◽  
Stem Cells ◽  
Therapeutic Potential ◽  
Mild Stress ◽  
Adipose Derived Stem Cells ◽  
Vitro Model ◽  
Novel Strategy ◽  
The One ◽  
Cell Conditioned Medium

Oxidative stress associated with neuroinflammation is a key process involved in the pathophysiology of neurodegenerative diseases, and therefore, has been proposed as a crucial target for new therapies. Recently, the therapeutic potential of human adipose-derived stem cells (hASCs) has been investigated as a novel strategy for neuroprotection. These cells can be preconditioned by exposing them to mild stress in order to improve their response to oxidative stress. In this study, we evaluate the therapeutic potential of hASCs preconditioned with low doses of H2O2 (called HC016 cells) to overcome the deleterious effect of oxidative stress in an in vitro model of oligodendrocyte-like cells (HOGd), through two strategies: i, the culture of oxidized HOGd with HC016 cell-conditioned medium (CM), and ii, the indirect co-culture of oxidized HOGd with HC016 cells, which had or had not been exposed to oxidative stress. The results demonstrated that both strategies had reparative effects, oxidized HC016 cell co-culture being the one associated with the greatest recovery of the damaged HOGd, increasing their viability, reducing their intracellular reactive oxygen species levels and promoting their antioxidant capacity. Taken together, these findings support the view that HC016 cells, given their reparative capacity, might be considered an important breakthrough in cell-based therapies.

scholarly journals Anti-senescence ion-delivering nanocarrier for recovering therapeutic properties of long-term-cultured human adipose-derived stem cells

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Yeong Hwan Kim ◽  
Gwang-Bum Im ◽  
Sung-Won Kim ◽  
Yu-Jin Kim ◽  
Taekyung Yu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Therapeutic Efficacy ◽  
Transition Metal Ions ◽  
Response To Treatment ◽  
Adipose Derived Stem Cells ◽  
Related Gene ◽  
Paracrine Factor ◽  
Therapeutic Properties ◽  
Factor Secretion

Abstract Background Human adipose-derived stem cells (hADSCs) have been used in various fields of tissue engineering because of their promising therapeutic efficacy. However, the stemness of hADSCs cannot be maintained for long durations, and their therapeutic cellular functions, such as paracrine factor secretion decrease during long-term cell culture. To facilitate the use of long-term-cultured hADSCs (L-ADSCs), we designed a novel therapeutic anti-senescence ion-delivering nanocarrier (AIN) that is capable of recovering the therapeutic properties of L-ADSCs. In the present study, we introduced a low-pH-responsive ion nanocarrier capable of delivering transition metal ions that can enhance angiogenic paracrine factor secretion from L-ADSCs. The AINs were delivered to L-ADSCs in an intracellular manner through endocytosis. Results Low pH conditions within the endosomes induced the release of transition metal ions (Fe) into the L-ADSCs that in turn caused a mild elevation in the levels of reactive oxygen species (ROS). This mild elevation in ROS levels induced a downregulation of senescence-related gene expression and an upregulation of stemness-related gene expression. The angiogenic paracrine factor secretion from L-ADSCs was significantly enhanced, and this was evidenced by the observed therapeutic efficacy in response to treatment of a wound-closing mouse model with conditioned medium obtained from AIN-treated L-ADSCs that was similar to that observed in response to treatment with short-term-cultured adipose-derived stem cells. Conclusions This study suggests a novel method and strategy for cell-based tissue regeneration that can overcome the limitations of the low stemness and therapeutic efficacy of stem cells that occurs during long-term cell culture. Graphical Abstract

scholarly journals Extracts from Myrtle Liqueur Processing Waste Modulate Stem Cells Pluripotency under Stressing Conditions

2019 ◽  
Vol 2019 ◽  
pp. 1-12 ◽  
Author(s):  
Sara Cruciani ◽  
Sara Santaniello ◽  
Angela Fadda ◽  
Luana Sale ◽  
Giorgia Sarais ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Stem Cells ◽  
Stem Cell ◽  
Pharmaceutical Preparations ◽  
Ros Production ◽  
Adipose Derived Stem Cells ◽  
Epigenetic Changes ◽  
Processing Waste ◽  
Stem Cell Pluripotency

Nutraceuticals present in food are molecules able to exert biological activity for the prevention and treatment of various diseases, in form of pharmaceutical preparations, such as capsules, cream, or pills. Myrtus communis L. is a spontaneous Mediterranean evergreen shrub, widely known for the liqueur obtained from its berries rich in phytochemicals such as tannins and flavonoids. In the present study, we aimed to evaluate the properties of myrtle byproducts, residual of the industrial liqueur processing, in Adipose-derived stem cells (ADSCs) induced at oxidative stress by in vitro H2O2 treatment. Cells were exposed for 12-24 and 48h at treatment with extracts and then senescence-induced. ROS production was then determined. The real-time PCR was performed to evaluate the expression of inflammatory cytokines and sirtuin-dependent epigenetic changes, as well the modifications in terms of stem cell pluripotency. The β-galactosidase assay was conducted to analyze stem cell senescence after treatment. Our results show that industrial myrtle byproducts retain a high antioxidant and antisenescence activity, protecting cells from oxidative stress damages. The results obtained suggest that residues from myrtle liqueur production could be used as resource in formulation of food supplements or pharmaceutical preparations with antioxidant, antiaging, and anti-inflammatory activity.

Evidence supporting neuroprotective effect of adipose derived stem cells on PC12 cells against oxidative stress induced by H2O2

2017 ◽  
Vol 63 (3) ◽  
pp. 1 ◽  
Author(s):  
M. T. Ghorabi ◽  
A. Aliaghaei ◽  
Y. Sadeghi ◽  
F. Shaerzadeh ◽  
A. A. Rad ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Stem Cells ◽  
Pc12 Cells ◽  
Neuroprotective Effect ◽  
Adipose Derived Stem Cells
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