scholarly journals The effect of Galleria mellonella hemolymph polypeptides on Legionella gormanii.

2014 ◽  
Vol 61 (1) ◽  
Author(s):  
Elżbieta Chmiel ◽  
Marta Palusinska-Szysz ◽  
Agnieszka Zdybicka-Barabas ◽  
Małgorzata Cytryńska ◽  
Paweł Mak

Among Legionella species, which are recognized to be pathogenic for humans, L. gormanii is the second prevalent causative agent of community-acquired pneumonia after L. pneumophila. Anti-L. gormanii activity of Galleria mellonella hemolymph extract and apolipophorin III (apoLp-III) was examined. The extract and apoLp-III at the concentration 0.025 mg/ml caused 75% and 10% decrease of the bacteria survival rate, respectively. The apoLp-III-induced changes of the bacteria cell surface were analyzed for the first time by atomic force microscopy. Our studies demonstrated the powerful anti-Legionella effects of the insect defence polypeptides, which could be exploited in drugs design against these pathogens.

Micron ◽  
2010 ◽  
Vol 41 (3) ◽  
pp. 220-226 ◽  
Author(s):  
Gi-Ja Lee ◽  
Eun-Jin Park ◽  
Samjin Choi ◽  
Jeong-Hoon Park ◽  
Kyung-Hwan Jeong ◽  
...  

Physiology ◽  
2002 ◽  
Vol 17 (6) ◽  
pp. 219-222 ◽  
Author(s):  
Bhanu P. Jena

Earlier electrophysiological measurements on live secretory cells suggested the presence of fusion pores at the plasma membrane, where secretory vesicles fuse to release vesicular contents. Recent studies using atomic force microscopy demonstrate for the first time the presence of the fusion pore and reveal its morphology and dynamics at near-nanometer resolution and in real time.


1999 ◽  
Vol 400 (1-3) ◽  
pp. 27-32 ◽  
Author(s):  
L.H Pope ◽  
M.C Davies ◽  
C.A Laughton ◽  
C.J Roberts ◽  
S.J.B Tendler ◽  
...  

Polymers ◽  
2021 ◽  
Vol 13 (22) ◽  
pp. 3922
Author(s):  
Xiaobin Liang ◽  
Makiko Ito ◽  
Ken Nakajima

In this study, atomic force microscopy (AFM) nanomechanics were used to visualize the nanoscale stress distribution in carbon black (CB)-reinforced isoprene rubber (IR) vulcanizates at different elongations and quantitatively evaluate their volume fractions for the first time. The stress concentrations in the protofibrous structure (stress chains) that formed around the CB filler in CB-reinforced IR vulcanizates were directly observed at the nanoscale. The relationship between the local nanoscale stress distribution and macroscopic tensile properties was revealed based on the microscopic stress distribution and microscopic spatial structure. This study can help us gain insight into the microscopic reinforcement mechanism of carbon black-containing rubber composites.


2020 ◽  
Vol 90 (11) ◽  
pp. 1843
Author(s):  
Р.В. Гайнутдинов ◽  
А.Л. Толстихина ◽  
Е.В. Селезнева ◽  
И.П. Макарова

Complex studies of new superprotonic crystals - acidic salts of potassium – ammonium sulfate (K1-x(NH4)x)3H(SO4)2, x ≥ 0.57 were carried out. Data on surface morphology, domain structure and conductivity of samples were obtained using atomic force microscopy. For the first time, the stability and degradation of the surface of superprotonic crystals were studied at the nanoscale. Based on piezoelectric response microscopy data, it was found that when the temperature decreased from 296 to 282 K, the (K0.43(NH4)0.57)3H(SO4)2 crystal transits from the paraelectric phase to the ferroelectric one.


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