scholarly journals Analysis of ciprofloxacin and gentamicin diffusion in Proteus mirabilis O18 biofilm by laser interferometry method.

1970 ◽  
Vol 60 (4) ◽  
Author(s):  
Michał Arabski ◽  
Sławomir Wąsik ◽  
Magdalena Zych ◽  
Wioletta Lakomiec ◽  
Wiesław Kaca

Laser interferometry is a measurement technique used in physical sciences, with a potential for new applications in microbiology. Our previously studies, focused on the quantitative analysis of antibiotics diffusion through membranes or their releasing from gel structure, indicate that this method might be useful in analysis of substances diffusion across the bacterial biofilms. As antibiotic - biofilm interaction model, we tested above method for determination of ciprofloxacin or gentamicin diffusion through Proteus mirabilis O18 biofilm. Laser interferometry analysis of antibiotics diffusion showed that the amount of ciprofloxacin transported through mature biofilm is 1.9 times higher than gentamicin. It was correlated with lower level of gentamicin in compare to the level of ciprofloxacin in biofilm, which amounts were predicted in biofilm during diffusion process by laser interferometry method. We suggest that the analysis of antibiotic diffusion in biofilm might by helpful in evaluation of effectiveness of antibacterial agents.

1967 ◽  
Vol 7 (2) ◽  
pp. 143-144 ◽  
Author(s):  
A. A. Ovsyannikov ◽  
L. S. Polak ◽  
R. E. Rovinskii ◽  
A. G. Rozanov ◽  
N. N. Shvindt

2015 ◽  
Vol 37 (1) ◽  
pp. 43-47
Author(s):  
Sławomir Wąsik ◽  
Michał Arabski ◽  
Karolina Maciejec ◽  
Grażyna Suchanek ◽  
Anna Świercz

The objective of the present study has been to test the laser interferometry method in terms of its usability for investigating sorption properties of minerals. This method was used to test the absorption capacity of halloysite with reference to glucose, which is often found in industrial wastewater and whose excess can disturb the environmental eco-balance. The sorption capacity of halloysite was thus determined indirectly, basing on the comparison of concentration profiles as well as time characteristics of glucose quantities released from the control solution and from the solution incubated with a halloysite adsorbent. An analysis of glucose diffusion was conducted in a two-chamber membrane system. On the basis of the obtained concentration profiles, the evolution of the concentration field was determined; so were the removal efficiency (%) and the amount of glucose adsorbed at equilibrium (qe, mg/g). The obtained results confirm good sorption properties of halloysite with respect to the investigated substance as well as usability of the method for this kind of investigations. The presented tests suggest that the measurement set-up can be optimised in such as way that visual rendering and testing the kinetics of the adsorbed substance direct release from the studied material become possible.


Polymers ◽  
2020 ◽  
Vol 12 (11) ◽  
pp. 2564
Author(s):  
Grzegorz Kowalski ◽  
Paweł Ptaszek ◽  
Łukasz Kuterasiński

In this paper, the authors discuss the results of research on the preparation and properties of superabsorbent hydrogels based on carboxymethylated high-amylose corn starches. They were obtained by graft copolymerisation (in an aqueous environment) of acrylic acid and its sodium salt onto modified starches (with various substitution degrees DS = 0.2 and 0.8), using potassium persulfate as an initiator and N,N’-methylenebisacrylamide (MBA) as a cross-linker. Modified starches, with various DS, were used to synthesise two series of hydrogels with varying molar ratios of cross-linkers to monomers. The swelling behaviour of hydrogels was studied and their properties were estimated using the kinetic equation. The occurrence of starch–polyacrylic acid covalent interactions was demonstrated by FTIR analysis. Nonlinear rheological methods have proved to be very effective in assessing the mechanical properties of hydrogels. LAOS (large -amplitude oscillatory shear) analysis allowed the determination of the durability of the gel structure as a function of the amount of absorbed water.


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