scholarly journals Synthesis of carotenoid-cysteine conjugates.

2012 ◽  
Vol 59 (1) ◽  
Author(s):  
Afshin Zand ◽  
Attila Agócs ◽  
József Deli ◽  
Veronika Nagy
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Human Liver ◽  
Water Solubility ◽  
Amino Group ◽  
Acid Moiety ◽  
Human Liver Cells ◽  
Allylic Cation ◽  
Amino Acid Moiety ◽  
Acidic Conditions

Isozeaxanthin under acidic conditions forms an allylic cation which reacts readily with thiol nucleophiles. With N-acetylcysteine as a nucleophile the products obtained are carotenoid-cysteine conjugates in which the amino acid moiety is attached to the carotenoid via sulphur in position 4. The water solubility of the products can be increased by deprotection of the amino group. The antioxidant activity of the products were examined on human liver cells under conditions of hydrogen-peroxide induced oxidative stress.

scholarly journals Protective effect ofLepidium sativumseed extract against hydrogen peroxide-induced cytotoxicity and oxidative stress in human liver cells (HepG2)

2015 ◽  
Vol 54 (2) ◽  
pp. 314-321 ◽  
Author(s):  
Ebtesam S. Al-Sheddi ◽  
Nida N. Farshori ◽  
Mai M. Al-Oqail ◽  
Javed Musarrat ◽  
Abdulaziz A. Al-Khedhairy ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Protective Effect ◽  
Human Liver ◽  
Liver Cells ◽  
Human Liver Cells ◽  
And Oxidative Stress

HBx or HCV core gene expression in HepG2 human liver cells results in a survival benefit against oxidative stress with possible implications for HCC development

2007 ◽  
Vol 168 (2) ◽  
pp. 128-134 ◽  
Author(s):  
Tamara Severi ◽  
Sara Vander Borght ◽  
Louis Libbrecht ◽  
Lucas VanAelst ◽  
Frederik Nevens ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Gene Expression ◽  
Survival Benefit ◽  
Human Liver ◽  
Core Gene ◽  
Liver Cells ◽  
Human Liver Cells

scholarly journals In Vitro Assessment of Cadmium Bioavailability in Chinese Cabbage Grown on Different Soils and Its Toxic Effects on Human Health

2015 ◽  
Vol 2015 ◽  
pp. 1-12 ◽  
Author(s):  
Rukhsanda Aziz ◽  
Muhammad Tariq Rafiq ◽  
Zhenli He ◽  
Di Liu ◽  
Kewang Sun ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Human Health ◽  
Chinese Cabbage ◽  
Human Liver ◽  
Liver Cells ◽  
Leafy Vegetables ◽  
Minimum Concentration ◽  
Human Liver Cells ◽  
Cadmium Bioavailability

The minimum concentration of cadmium (Cd), by Chinese cabbage grown on Cd contaminated soils that can initiate toxicity in human liver cells using in vitro digestion coupled with Caco-2/HL-7702 cell models was studied. Cadmium bioaccessibility in the gastric phase for yellow soil (YS) cabbage (40.84%) and calcareous soil (CS) cabbage (21.54%) was significantly higher than small intestinal phase with the corresponding values of 21.2% and 11.11%, respectively. Cadmium bioavailability was higher in YS cabbage (5.27%–14.66%) than in CS cabbage (1.12%–9.64%). Cadmium concentrations (>0.74 μg) transported from YS and CS cabbage were able to induce oxidative (MDA, H2O2) stress by inhibiting antioxidant (SOD, GPx) enzyme activities in human liver cells (HL-7702). Additionally the study revealed that the ingestion of Cd contaminated Chinese cabbage grown in acidic soil (yellow soil) weakened the antioxidant defense system under all levels of contamination (2, 6, and 9 mg·kg−1) which ultimately escalated the oxidative stress in liver cells; however, in case of CS cabbage, a marked oxidative stress was observed only at 9 mg kg−1Cd level of soil. Therefore, it is necessary to monitor Cd concentrations in leafy vegetables grown on acidic soils to minimize human health risk.

Hydrolysis of some natural and synthetic bile acid conjugates by Cercospora melonis

1982 ◽  
Vol 28 (5) ◽  
pp. 457-461 ◽  
Author(s):  
M. R. Johns ◽  
R. Chong ◽  
I. S. Maddox
Keyword(s):  
Bile Acid ◽  
Bile Acids ◽  
Cell Cultures ◽  
Acid Group ◽  
Amino Group ◽  
Acid Moiety ◽  
Whole Cell ◽  
Amino Acid Moiety ◽  
Two Factors ◽  
Hydrolysis Of

Analogs of natural bile acid conjugates were incubated with both whole-cell cultures and cell-free extracts of the fungus Cercospora melonis CBS 162.60. Those conjugates containing both an α-amino group and a carboxylic acid group in the amino acid moiety were the most susceptible to hydrolysis. Substitution with a β-amino group and (or) a sulfonic acid group greatly reduced the extent of hydrolysis. With all conjugates tested, significant losses of total bile acid were observed on incubation with whole-cell cultures. Two factors have been implicated as contributing to these losses. First, bile acids may be degraded to nonsteroidal products by an inducible enzyme system. Second, bile acids may be bound to mycelia in a manner not reversed by the extraction technique.

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