scholarly journals Lycopene recovery from tomato peel under mild conditions assisted by enzymatic pre-treatment and non-ionic surfactants.

2012 ◽  
Vol 59 (1) ◽  
Author(s):  
Emmanouil H Papaioannou ◽  
Anastasios J Karabelas
Keyword(s):  
Extraction Yield ◽  
Molar Ratio ◽  
Attractive Alternative ◽  
Enzymatic Pretreatment ◽  
Enzyme Preparations ◽  
Assisted Extraction ◽  
Tomato Processing ◽  
Pretreatment Time ◽  
Pre Treatment ◽  
Surfactant Assisted

The tomato processing industry generates large quantities of tomato peel residues, usually creating environmental problems. These residues are a significant source of lycopene, thus providing an attractive alternative for profitable handling of these otherwise problematic by-products. The enzymatic pretreatment of these residues for lycopene recovery has already been employed, although the use of surfactants for enhancing the recovery has not been examined so far. The enzymatic pretreatment of tomato peels, using two commercially available pectinolytic enzyme preparations, was evaluated suggesting that there is an optimum pretreatment time of about 1 h, enzyme amount 250 Units/mL and no significant pH influence. Lycopene surfactant - assisted extraction was further investigated, showing that, among eight surfactants used, the most suitable was "Span 20", with an optimum ratio of 6-7 surfactant molecules per lycopene molecule. Sequential enzymatic pretreatment and surfactant-assisted extraction (30 min for each step) was evaluated leading to an improved lycopene extraction yield, with a somewhat smaller surfactant molar ratio (i.e. 4-5). In the latter case, the yield of lycopene recovery was almost four times greater compared to just 1 hr enzymatic pretreatment, and was approximately ten times greater compared to the recovery from untreated peels. Furthermore, such lipophilic compound recovery, avoiding the use of organic solvents, is environmentally attractive and ensures direct lycopene use in the food and cosmetics industries.

scholarly journals Deep Eutectic Solvent-Based Microwave-Assisted Extraction of Baicalin from Scutellaria baicalensis Georgi

2018 ◽  
Vol 2018 ◽  
pp. 1-10 ◽  
Author(s):  
Hui Wang ◽  
Xiaodi Ma ◽  
Qibin Cheng ◽  
Xiaoli Xi ◽  
Liwei Zhang
Keyword(s):  
Maximum Yield ◽  
Deep Eutectic Solvent ◽  
Extraction Yield ◽  
Scutellaria Baicalensis ◽  
Molar Ratio ◽  
Extraction Temperature ◽  
Microwave Assisted Extraction ◽  
Scutellaria Baicalensis Georgi ◽  
Microwave Assisted ◽  
Assisted Extraction

Deep eutectic solvents (DESs) have attracted significant attention as green media for the extraction and separation of natural compounds from Chinese medicine. In this study, a hydrophobic DESs-based microwave-assisted extraction (MAE) was successfully used to efficiently extract baicalin from Scutellaria baicalensis Georgi. Firstly, DecA: N4444-Cl (DES-1 , molar ratio 1 : 2) was screened and selected as the most appropriate DES by comparing the extraction yield in different hydrophobic DESs. Based on the extraction yield of baicalin, response surface methodology (RSM) was employed to model and optimize the parameters (extraction temperature, liquid-solid ratio, and extraction time). Furthermore, the maximum yield of 106.96 mg·g−1 was achieved under optimum conditions in DES-containing aqueous solutions (33 vol% water content), which reached a similar level that was conducted using the pharmacopoeia procedure (104.94 mg·g−1). These results indicated that the proposed method is an excellent alternative for the extraction of baicalin.

scholarly journals Advantageous Preparation of Digested Proteic Extracts from Spirulina platensis Biomass

Catalysts ◽  
2019 ◽  
Vol 9 (2) ◽  
pp. 145 ◽  
Author(s):  
Carlos Verdasco-Martín ◽  
Lea Echevarrieta ◽  
Cristina Otero
Keyword(s):  
Extraction Methods ◽  
Extraction Yield ◽  
Efficient Production ◽  
Cell Integrity ◽  
Food Ingredients ◽  
Operation Conditions ◽  
Transmission Electron ◽  
Assisted Extraction ◽  
Pharmaceutical Industries ◽  
Pre Treatment

Spirulina biomass has great nutritional value, but its proteins are not as well adsorbed as animal ones are. New functional food ingredients and metabolites can be obtained from spirulina, using different selective biodegradations of its biomass. Four enzyme-assisted extraction methods were independently studied, and their best operation conditions were determined. Enzymes were employed to increase the yield of easily adsorbed proteic extracts. A biomass pre-treatment using Alcalase® (pH 6.5, 1% v/w, and 30 °C) is described, which increased the extraction yield of hydrophilic biocomponents by 90% w/w compared to the simple solvent extraction. Alcalase® gives rise to 2.5–6.1 times more amino acids than the others and eight differential short peptides (438–1493 Da). These processes were scaled up and the extracts were analyzed. Higher destruction of cell integrity in the case of Alcalase® was also visualized by transmission electron microscopy. The described extractive technology uses cheap, commercial, food grade enzymes and hexane, accepted for food and drug safety. It is a promising process for a competitive biofactory, thanks to an efficient production of extracts with high applied potential in the nutrition, cosmetic, and pharmaceutical industries.

A Technological Research on Synergetic Extraction of Momordica charantia L. Saponin Using Microwave-Surfactant

2014 ◽  
Vol 563 ◽  
pp. 384-390
Author(s):  
Tang Kai ◽  
Yan Jie ◽  
Huang Xin
Keyword(s):  
Surfactant Concentration ◽  
Extraction Yield ◽  
Influential Factor ◽  
Extraction Time ◽  
Technological Research ◽  
Orthogonal Experiments ◽  
Assisted Extraction ◽  
Main Factors ◽  
Optimum Extraction ◽  
Surfactant Assisted

In this paper, microwave-surfactant assisted extraction of saponin from Momordica Charantia.L was studied. The content of saponin was determined by the method of vanillin-acetic acid and perchloric which were used as developer. Four main factors including extraction powder, extraction time, ratio of solid to liquid and surfactant concentration were studied by orthogonal experiments. Results showed that extraction powder was the most significant influential factor, followed by extraction time and ratio of solid to liquid. Surfactant concentration showed the minimum influence. The optimum extraction conditions were: extraction powder 400W, extraction time 5min, ratio of solid to liquid 1:12g/mL and surfactant concentration 0.03mol/L, under which the extraction yield of saponin reached 4.69%.

scholarly journals Microwave assisted extraction of essential oils from enzymatically pretreated lavender (Lavandula angustifolia Miller)

2014 ◽  
Vol 12 (8) ◽  
pp. 829-836 ◽  
Author(s):  
Ioan Calinescu ◽  
Adina Gavrila ◽  
Maria Ivopol ◽  
Gabriel Ivopol ◽  
Mariana Popescu ◽  
...  
Keyword(s):  
Essential Oils ◽  
Enzyme Concentration ◽  
Extraction Yield ◽  
Microwave Assisted Extraction ◽  
Lavandula Angustifolia ◽  
Enzymatic Pretreatment ◽  
23 Factorial Design ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Factorial Design Of Experiments

AbstractIn this study, microwave assisted a hydrodistillation process (MWHD) of essential oils from lavender (Lavandula angustifolia Miller) was investigated. In order to examine any potential differences in essential oil extraction, the lavender flowers underwent enzymatic pretreatment.A 23 factorial design of experiments, combined with statistical methods of data analysis were used to optimize enzymatic pretreatment and to evaluate the influence of major variables (enzyme concentration, temperature and pH) on the performance of the microwave assisted extraction.Under optimal conditions, an extraction yield of 24 mg oil g−1 substrate was achieved (an increase by approximately 25% in comparison with the classic extraction conditions of conventional hydrodistillation).The main compounds of the essential oils obtained were analyzed and identified by gas chromatography coupled to mass spectrometry (GC-MS). Analyzing the data obtained indicated that the content of main compounds (linalool and linalyl acetate − 73%) was greater than that obtained by conventional extraction (67%).

Optimization of Enzyme-Assisted Lycopene Extraction from Tomato Processing Waste

2013 ◽  
Vol 800 ◽  
pp. 173-176 ◽  
Author(s):  
Antonio Zuorro ◽  
Roberto Lavecchia
Keyword(s):  
Fold Increase ◽  
Polynomial Equation ◽  
Cellulolytic Enzyme ◽  
Extraction Temperature ◽  
Processing Waste ◽  
Enzyme Preparations ◽  
Solid Ratio ◽  
Five Factors ◽  
Tomato Processing ◽  
Pretreatment Time

We have investigated the recovery of lycopene from tomato processing waste (TPW) by treating the peel fraction of the waste with pectinolytic and cellulolytic enzyme preparations. To optimize the enzyme-assisted extraction, we used a two-level factorial design with five factors: extraction temperature (T), pretreatment time (P), extraction time (E), enzyme solution-to-solid ratio (R), enzyme load (L). A 7-to 16-fold increase was observed in lycopene recovery, compared to the untreated TPW. From a response surface analysis of the data, a second-degree polynomial equation was developed which provided the following optimal extraction conditions: T = 30 °C, P = 3.5 h, E = 3.2 h, R = 30 dm3/kg, L = 0.17 kg/kg.

scholarly journals Enzymatic Pretreatment of Vegetable Materials to Increase the Extraction Yield of Bioactive Compounds

2018 ◽  
Vol 69 (11) ◽  
pp. 3271-3274
Author(s):  
Ioana Asofiei ◽  
Ioan Calinescu ◽  
Petre Chipurici ◽  
Adina Ionuta Gavrila
Keyword(s):  
Phenolic Content ◽  
Extraction Process ◽  
Extraction Yield ◽  
Extraction Time ◽  
Total Phenolic ◽  
Extraction Temperature ◽  
Enzymatic Pretreatment ◽  
Stirring Rate ◽  
Parameters Extraction ◽  
Assisted Extraction

In this paper, the influence of enzymatic pretreatment on the microwave assisted extraction (MAE) of polyphenols from artichoke leaves is described. Prior to enzymatic pretreatment, the influence of different parameters (extraction time, stirring rate, and extraction temperature) on the extraction process was studied. The total phenolic content (TPC) increases with the stirring rate. To avoid degradation of polyphenols, the extraction time and temperature should not be too high. The antioxidant capacity is in concordance with the TPC results. The enzymatic pretreatment, for the best extraction conditions, enhances the concentration of polyphenols compared with the extracts obtained without pretreatment.

Enzyme-assisted Extraction for Optimized Recovery of Phenolic Bioactives from Peganum hermala Leaves Using Response Surface Methodology

2018 ◽  
Vol 17 (4) ◽  
pp. 349-354
Author(s):  
Qadir Rahman ◽  
Anwar Farooq ◽  
Amjad Gilani Mazhar ◽  
Nadeem Yaqoob Muhammad ◽  
Ahmad Mukhtar
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Enzyme Concentration ◽  
Coumaric Acid ◽  
Assisted Extraction ◽  
Pre Treatment ◽  
Optimized Conditions ◽  
Enzyme Complexes ◽  
Hydrolysis Of ◽  
Central Composite

This study investigates the effect of enzyme formulations (Zympex-014, Kemzyme dry-plus and Natuzyme) on recovery of phenolics from Peganum hermala (harmal) leaves, under optimized conditions using response surface methodology. As compared to the other enzyme complexes, the yield (34 g/100g) obtained through Zympex-014-assisted extraction was higher under optimized conditions such as time (75 min), temperature (70°C), pH (6.5) and enzyme concentration (5 g/100 g) using central composite design (CCD). Effectiveness of Zympex-014 towards hydrolysis of P. hermala leaves cell wall was examined by analyzing the control and enzyme-treated leave residues using scanning electron microscope (SEM). GC/MS characterization authenticated the presence of quercetin (1.44), gallic acid (0.23), caffeic acid (0.04), cinnamic acid (0.05), m-coumaric acid (0.23) and p-coumaric acid (0.37 μg/g) as the potent phenolics in Zympex-014 based extract. It can be concluded from the findings of the current work that pre-treatment of P. hermala leaves with Zympex-014 significantly enhanced the recovery of phenolics that supports its potential uses in the nutra-pharamaceutical industry.

Microwave Pretreatment of Vegetable Materials to Increase the Extraction Yield of Natural Products

2018 ◽  
Vol 69 (8) ◽  
pp. 1976-1979
Author(s):  
Ioana Asofiei ◽  
Ioan Calinescu ◽  
Adina Ionuta Gavrila ◽  
Daniel Ighigeanu ◽  
Diana Martin
Keyword(s):  
Natural Products ◽  
Chemical Composition ◽  
Phenolic Compounds ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Extraction Yield ◽  
Total Phenolic ◽  
Microwave Pretreatment ◽  
Pretreatment Time ◽  
Conventional Extraction

It was designed and built a laboratory experimental installation (LEI) for the microwave pretreatment of vegetable materials. To study the influence of microwave pretreatment on the total phenolic content (TPC), a conventional extraction of polyphenols from treated and untreated fresh sea buckthorn leaves was performed. For short extraction times, the amount of phenolic compounds was higher for the extracts obtained from treated leaves, but a long pretreatment time (28 s) led to a decrease in TPC. The qualitative analysis showed that the chemical composition is not affected by the microwave pretreatment.

scholarly journals Mathematical Modelling of Ultrasound-Assisted Extraction Kinetics of Bioactive Compounds from Artichoke By-Products

Foods ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 931
Author(s):  
Cristina Reche ◽  
Carmen Rosselló ◽  
Mónica M. Umaña ◽  
Valeria Eim ◽  
Susana Simal
Keyword(s):  
Power Density ◽  
Bioactive Compounds ◽  
Extraction Yield ◽  
Total Phenolic ◽  
Model Parameters ◽  
Ultrasound Assisted Extraction ◽  
Yield Curves ◽  
Assisted Extraction ◽  
Ultrasound Assisted ◽  
Ultrasound Power

Valorization of an artichoke by-product, rich in bioactive compounds, by ultrasound-assisted extraction, is proposed. The extraction yield curves of total phenolic content (TPC) and chlorogenic acid content (CAC) in 20% ethanol (v/v) with agitation (100 rpm) and ultrasound (200 and 335 W/L) were determined at 25, 40, and 60 °C. A mathematical model considering simultaneous diffusion and convection is proposed to simulate the extraction curves and to quantify both temperature and ultrasound power density effects in terms of the model parameters variation. The effective diffusion coefficient exhibited temperature dependence (72% increase for TPC from 25 °C to 60 °C), whereas the external mass transfer coefficient and the equilibrium extraction yield depended on both temperature (72% and 90% increases for TPC from 25 to 60 °C) and ultrasound power density (26 and 51% increases for TPC from 0 (agitation) to 335 W/L). The model allowed the accurate curves simulation, the average mean relative error being 5.3 ± 2.6%. Thus, the need of considering two resistances in series to satisfactorily simulate the extraction yield curves could be related to the diffusion of the bioactive compound from inside the vegetable cells toward the intercellular volume and from there, to the liquid phase.

Characterization of water soluble pentosans of enzyme supplemented dough and breads/ Caracterización de las pentosanas solubles en agua extraídas de masa y pan conteniendo enzimas comerciales

2000 ◽  
Vol 6 (3) ◽  
pp. 197-205 ◽  
Author(s):  
T. Jimenez ◽  
M.A. Martinez-Anaya
Keyword(s):  
Molecular Weight ◽  
Ferulic Acid ◽  
Synergistic Effects ◽  
Extraction Yield ◽  
Water Soluble ◽  
Sugar Composition ◽  
Enzyme Preparations ◽  
Processing Enzyme ◽  
Enzyme Source

Water soluble pentosans (WSP) from doughs and breads made with different enzyme preparations are characterized according to extraction yield, sugar composition, xylose/arabinose ratio and molecular weight (MW) distribution. Extraction yield was greater for dough than for bread samples, ranging from 0.94 to 1.64%, but bread extracts had a higher purity. Percent of pentoses in purified WSP was greater in pentosanase supplemented samples (28-55%) than in control and amylase containing samples (23-32%). Major sugars were xylose and arabinose, but glucose and mannose also appeared in the extracts. The xylose/arabinose (Xyl/Ara) ratio was 1.3-1.6 and underwent small changes during processing. Enzyme addition caused an increase in Xyl/Ara ratio, attributable to a debranching of arabinoxylans (AX) with higher degree of Ara substitution by arabinofuranosidase. Addition of pentosanases had a significant effect in increasing WSP with MW over 39 000, whereas those of low MW changed only slightly. MW distribution depended on enzyme source, and whereas some enzymes showed activity during fermentation others increased their activity during baking. No synergistic effects were observed in studied variables due to the combination of amylases with pentosanases. Protein in WSP extracts eluted together with ferulic acid suggesting they were linked, but not associated with a determined carbohydrate fraction.

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