scholarly journals Cytotoxic effects of cladribine and tezacitabine toward HL-60.

2005 ◽  
Vol 52 (2) ◽  
pp. 561-565 ◽  
Author(s):  
Krzysztof Stachnik ◽  
Paweł Grieb ◽  
Janusz S Skierski
Keyword(s):  
Flow Cytometry ◽  
Cell Death ◽  
Growth Inhibition ◽  
Microscopic Observation ◽  
Cell Counting ◽  
Cytotoxic Effects ◽  
Massive Cell Death ◽  
Induction Of Apoptosis ◽  
Massive Cell ◽  
Direct Microscopic Observation

The aim of the study was to determine the relation between the cytotoxic and cytostatic effects of tezacitabine and cladribine on a HL-60 cell line and the time of exposure of cells to these drugs. Cell viability and induction of apoptosis were assessed using flow cytometry methods. Apoptosis was confirmed by direct microscopic observation. Growth inhibition was examined by cell counting. After 24 h incubation tezacitabine was equally or less toxic compared to cladribine. However, toxicity of tezacitabine strongly rose after 48 h incubation leading to massive cell death at doses much lower than those of cladribine. Assessment of the effect of increased exposure time on the clinical efficacy of tezacitabine is indicated.

scholarly journals Cytotoxicity of Environmentally Relevant Concentrations of Aluminum in Murine Thymocytes and Lymphocytes

2011 ◽  
Vol 2011 ◽  
pp. 1-7 ◽  
Author(s):  
Jamal Kamalov ◽  
David O. Carpenter ◽  
Irina Birman
Keyword(s):  
Flow Cytometry ◽  
Cell Death ◽  
Immune System ◽  
Dna Binding ◽  
Aluminum Chloride ◽  
Cell Swelling ◽  
Cytotoxic Effects ◽  
Minute Exposure ◽  
Low Concentrations ◽  
Dose Dependent

The effects of low concentrations of aluminum chloride on thymocytes and lymphocytes acutely dissociated from young mice were studied using flow cytometry with a DNA-binding dye. We demonstrate a rapid and dose-dependent injury in murine thymocytes and lymphocytes resulting from exposure to aluminum, as indicated by an increase in the entry into the cell of the DNA-binding dye, propidium iodine. A 60-minute exposure to 10 μM AlCl3caused damage of about 5% of thymocytes, while 50% were injured after 10 minutes at 20 μM. Nearly all thymocytes showed evidence of damage at 30 μM AlCl3after only 5 minutes of incubation. In lymphocytes, injury was observed at 15 μM AlCl3and less than 50% of cells were injured after a 60-minute exposure to 20 μM. Injury only rarely proceeded to rapid cell death and was associated with cell swelling. These results suggest that aluminum has cytotoxic effects on cells of the immune system.

scholarly journals Loss of Drosophila E3 Ubiquitin Ligase Hyd Promotes Extra Mitosis in Germline Cysts and Massive Cell Death During Oogenesis

2020 ◽  
Vol 8 ◽  
Author(s):  
Natalia V. Dorogova ◽  
Yuliya A. Galimova ◽  
Elena Us. Bolobolova ◽  
Elina M. Baricheva ◽  
Svetlana A. Fedorova
Keyword(s):  
Cell Death ◽  
Ubiquitin Ligase ◽  
E3 Ubiquitin Ligase ◽  
Massive Cell Death ◽  
Massive Cell

55Reintroduction of bladder cancer-downregulated miR-145 results in massive cell death of bladder cancer cell lines

Apmis ◽  
2008 ◽  
Vol 116 (5) ◽  
pp. 417-417
Author(s):  
Marie S. Ostenfeld ◽  
Jesper B. Bramsen ◽  
Jørgen Kjems ◽  
Torben Ørntoft ◽  
Lars Dyrskjøt
Keyword(s):  
Bladder Cancer ◽  
Cell Death ◽  
Cell Lines ◽  
Cancer Cell ◽  
Cancer Cell Lines ◽  
Bladder Cancer Cell ◽  
Massive Cell Death ◽  
Massive Cell

scholarly journals The Bispidinone Derivative 3,7-Bis-[2-(S)-amino-3-(1H-indol-3-yl)-propionyl]-1,5-diphenyl-3,7-diazabicyclo[3.3.1]nonan-9-one Dihydrochloride Induces an Apoptosis-Mediated Cytotoxic Effect on Pancreatic Cancer Cells In Vitro

Molecules ◽  
2019 ◽  
Vol 24 (3) ◽  
pp. 524 ◽  
Author(s):  
Melanie Predebon ◽  
Danielle Bond ◽  
Joshua Brzozowski ◽  
Helen Jankowski ◽  
Fiona Deane ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Flow Cytometry ◽  
Cell Death ◽  
Cell Lines ◽  
Cytotoxic Effect ◽  
Treatment Time ◽  
Apoptotic Cell ◽  
Apoptotic Cell Death ◽  
Cytotoxic Agents ◽  
Cell Counting

Pancreatic cancer (PC) is a complex, heterogeneous disease with a dismal prognosis. Current therapies have failed to improve survival outcomes, urging the need for discovery of novel targeted treatments. Bispidinone derivatives have yet to be investigated as cytotoxic agents against PC cells. The cytotoxic effect of four bispidinone derivatives (BisP1: 1,5-diphenyl-3,7-bis(2-hydroxyethyl)-3,7-diazabicyclo[3.3.1]nonan-9-one; BisP2: 3,7-bis-(2-(S)-amino-4-methylsulfanylbutyryl)-1,5-diphenyl-3,7-diazabicyclo[3.3.1]nonan-9-one dihydrochloride; BisP3: [2-{7-[2-(S)-tert-butoxycarbonylamino-3-(1H-indol-3-yl)-propionyl]-9-oxo-1,5-diphenyl-3,7-diazabicyclo[3.3.1]non-3-yl}-1-(S)-(1H-indol-3-ylmethyl)-2-oxoethyl]-carbamic acid tertbutyl ester; BisP4: 3,7-bis-[2-(S)-amino-3-(1H-indol-3-yl)-propionyl]-1,5-diphenyl-3,7-diazabicyclo[3.3.1]nonan-9-one dihydrochloride) was assessed against PC cell lines (MiaPaca-2, CFPAC-1 and BxPC-3). Cell viability was assessed using a Cell Counting Kit-8 (CCK-8) colorimetric assay, while apoptotic cell death was confirmed using fluorescence microscopy and flow cytometry. Initial viability screening revealed significant cytotoxic activity from BisP4 treatment (1 µM–100 µM) on all three cell lines, with IC50 values for MiaPaca-2, BxPC-3, and CFPAC-1 16.9 µM, 23.7 µM, and 36.3 µM, respectively. Cytotoxic treatment time-response (4 h, 24 h, and 48 h) revealed a 24 h treatment time was sufficient to produce a cytotoxic effect on all cell lines. Light microscopy evaluation (DAPI staining) of BisP4 treated MiaPaca-2 PC cells revealed dose-dependent characteristic apoptotic morphological changes. In addition, flow cytometry confirmed BisP4 induced apoptotic cell death induction of activated caspase-3/-7. The bispidinone derivative BisP4 induced an apoptosis-mediated cytotoxic effect on MiaPaca-2 cell lines and significant cytotoxicity on CFPAC-1 and BxPC-3 cell lines. Further investigations into the precise cellular mechanisms of action of this class of compounds are necessary for potential development into pre-clinical trials.

scholarly journals Methylene blue photodynamic therapy induces selective and massive cell death in human breast cancer cells

BMC Cancer ◽  
2017 ◽  
Vol 17 (1) ◽  
Author(s):  
Ancély F. dos Santos ◽  
Letícia F. Terra ◽  
Rosangela A. M. Wailemann ◽  
Talita C. Oliveira ◽  
Vinícius de Morais Gomes ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Methylene Blue ◽  
Cell Death ◽  
Photodynamic Therapy ◽  
Human Breast Cancer ◽  
Breast Cancer Cells ◽  
Human Breast Cancer Cells ◽  
Human Breast ◽  
Massive Cell Death ◽  
Massive Cell

55 Reintroduction of bladder cancer-downregulated miR-145 results in massive cell death of bladder cancer cell lines

Apmis ◽  
2008 ◽  
Vol 116 (5) ◽  
pp. 417-417
Author(s):  
Marie S. Ostenfeld ◽  
Jesper B. Bramsen ◽  
Jørgen Kjems ◽  
Torben Ørntoft ◽  
Lars Dyrskjøt
Keyword(s):  
Bladder Cancer ◽  
Cell Death ◽  
Cell Lines ◽  
Cancer Cell ◽  
Cancer Cell Lines ◽  
Bladder Cancer Cell ◽  
Massive Cell Death ◽  
Massive Cell

scholarly journals Dexamethasone Treatment Limits Efficacy of Radiation, but Does Not Interfere With Glioma Cell Death Induced by Tumor Treating Fields

2021 ◽  
Vol 11 ◽  
Author(s):  
Benedikt Linder ◽  
Abigail Schiesl ◽  
Martin Voss ◽  
Franz Rödel ◽  
Stephanie Hehlgans ◽  
...  
Keyword(s):  
Cell Death ◽  
Cell Lines ◽  
Clinical Analysis ◽  
Concomitant Treatment ◽  
Tumor Treating Fields ◽  
Cell Counts ◽  
Anti Cancer ◽  
Massive Cell Death ◽  
Human Gbm ◽  
Massive Cell

PurposeDexamethasone (Dex) is the most common corticosteroid to treat edema in glioblastoma (GBM) patients. Recent studies identified the addition of Dex to radiation therapy (RT) to be associated with poor survival. Independently, Tumor Treating Fields (TTFields) provides a novel anti-cancer modality for patients with primary and recurrent GBM. Whether Dex influences the efficacy of TTFields, however, remains elusive.MethodsHuman GBM cell lines MZ54 and U251 were treated with RT or TTFields in combination with Dex and the effects on cell counts and cell death were determined via flow cytometry. We further performed a retrospective analysis of GBM patients with TTFields treatment +/- concomitant Dex and analysed its impact on progression-free (PFS) and overall survival (OS).ResultsThe addition of Dex significantly reduced the efficacy of RT in U251, but not in MZ54 cells. TTFields (200 kHz/250 kHz) induced massive cell death in both cell lines. Concomitant treatment of TTFields and Dex did not reduce the overall efficacy of TTFields. Further, in our retrospective clinical analysis, we found that the addition of Dex to TTFields therapy did not influence PFS nor OS.ConclusionOur translational investigation indicates that the efficacy of TTFields therapy in patients with GBM and GBM cell lines is not affected by the addition of Dex.

scholarly journals Theranostic Role of 32P-ATP as Radiopharmaceutical for the Induction of Massive Cell Death within Avascular Tumor Core

Theranostics ◽  
2017 ◽  
Vol 7 (18) ◽  
pp. 4399-4409 ◽  
Author(s):  
Mirco Galiè ◽  
Federico Boschi ◽  
Ilaria Scambi ◽  
Flavia Merigo ◽  
Pasquina Marzola ◽  
...  
Keyword(s):  
Cell Death ◽  
Massive Cell Death ◽  
Massive Cell ◽  
Avascular Tumor
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